FastQCFastQC Report
Wed 25 May 2016
SRR1033196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944878
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53450.18150157663577235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43040.14615206470352932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.053.394631
GTATCAA75150.042.880841
TCAACGC85200.036.911664
ATCAACG85750.036.7297253
CAACGCA88250.035.742495
TATCAAC92200.034.5177272
AACGCAG92400.034.2804836
ACGCAGA106750.029.4521687
CGCAGAG108650.028.8058978
GTACATG74300.026.5926861
TACATGG72550.026.0479222
GCAGAGT120350.025.7707679
ACATGGG77900.023.59483
GTGGTAT20550.022.4344771
GAGTACT84550.022.39860512-13
CAGAGTA120900.021.16416410-11
AGAGTAC111450.020.55531110-11
TACACCG2601.6820195E-819.888245
GGTCGCG1909.056041E-619.7877047
ATACCGC2151.1963166E-619.672666
Produced by FastQC (version 0.11.5)