FastQCFastQC Report
Wed 25 May 2016
SRR1033195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197151
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG144200.028.2564011
GTATCAA78100.027.9251791
TACATGG147500.026.776872
TCAACGC79200.026.3979824
CAACGCA81350.025.7006155
ACATGGG148200.025.398513
AACGCAG83300.025.2576486
ATCAACG83200.025.1904243
TATCAAC87550.024.3284592
ACGCAGA97500.021.1455927
CATGGGG120650.021.028214
GGTATCA60650.020.426361
CGCAGAG102700.019.8462838
GAGTACA97050.019.7544481
TATACTG15750.019.091525
GTATAGG12200.018.9191781
AGTACAT100400.018.2174852
GCAGAGT114400.017.159729
GTATATA18200.017.0819931
GTATAGA15200.016.4247321
Produced by FastQC (version 0.11.5)