##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033195_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4197151
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.824378965636452	33.0	31.0	34.0	30.0	34.0
2	31.920109378957296	34.0	31.0	34.0	30.0	34.0
3	32.071183285995666	34.0	31.0	34.0	30.0	34.0
4	35.475498260605825	37.0	35.0	37.0	35.0	37.0
5	35.54819590717608	37.0	35.0	37.0	35.0	37.0
6	35.674333136930265	37.0	37.0	37.0	35.0	37.0
7	35.604903897905984	37.0	37.0	37.0	35.0	37.0
8	35.61543008578914	37.0	37.0	37.0	35.0	37.0
9	37.33771789482913	39.0	38.0	39.0	35.0	39.0
10-11	37.31876206026421	39.0	38.0	39.0	35.0	39.0
12-13	37.28807576853919	39.0	38.0	39.0	35.0	39.0
14-15	38.76702029543374	41.0	39.0	41.0	36.0	41.0
16-17	38.763321596006435	41.0	39.0	41.0	36.0	41.0
18-19	38.73445415711753	41.0	39.0	41.0	36.0	41.0
20-21	38.752802794085795	41.0	39.0	41.0	35.5	41.0
22-23	38.70447727518024	41.0	39.0	41.0	35.0	41.0
24-25	38.679672234808805	41.0	39.0	41.0	35.0	41.0
26-27	38.62488173525327	41.0	39.0	41.0	35.0	41.0
28-29	38.58531572964613	40.5	39.0	41.0	34.5	41.0
30-31	38.57730851236946	41.0	39.0	41.0	35.0	41.0
32-33	38.64341835688066	41.0	39.0	41.0	35.0	41.0
34-35	38.60385699728221	41.0	39.0	41.0	35.0	41.0
36-37	38.51917657954169	41.0	39.0	41.0	34.5	41.0
38-39	38.43555652393731	41.0	39.0	41.0	34.0	41.0
40-41	38.35951506152626	41.0	38.5	41.0	34.0	41.0
42-43	38.2289271937083	40.0	38.0	41.0	34.0	41.0
44-45	38.07751400890747	40.0	38.0	41.0	33.0	41.0
46-47	37.928027726426805	40.0	38.0	41.0	33.0	41.0
48-49	37.78990438990639	40.0	37.5	41.0	33.0	41.0
50-51	37.63845153533909	40.0	37.0	41.0	33.0	41.0
52-53	37.465663017604086	40.0	37.0	41.0	33.0	41.0
54-55	37.28234581028893	40.0	36.0	41.0	32.0	41.0
56-57	37.08411562986416	40.0	36.0	41.0	32.0	41.0
58-59	36.85782379523634	39.0	35.0	41.0	31.5	41.0
60-61	36.602529191825596	39.0	35.0	41.0	31.0	41.0
62-63	36.31147044745352	38.5	35.0	41.0	31.0	41.0
64-65	36.00915859353166	38.0	35.0	40.0	31.0	41.0
66-67	35.68807317153946	37.0	35.0	40.0	30.5	41.0
68-69	35.357357407441384	37.0	35.0	39.5	30.0	41.0
70-71	34.99857093537973	36.0	34.5	39.0	30.0	41.0
72-73	34.627940834151545	36.0	34.0	39.0	30.0	41.0
74-75	34.25750658005872	35.0	34.0	38.0	29.0	40.0
76-77	33.94426111903051	35.0	34.0	37.0	29.0	39.0
78-79	33.60127488860896	35.0	34.0	37.0	29.0	39.0
80-81	33.27443651657994	35.0	34.0	36.0	29.0	39.0
82-83	33.11411228712048	35.0	34.0	36.0	29.0	37.0
84-85	32.88937936709925	35.0	34.0	35.5	29.0	37.0
86-87	32.69130846138249	35.0	34.0	35.0	29.0	36.5
88-89	32.39082916006596	35.0	34.0	35.0	27.5	36.0
90-91	32.24206825058236	35.0	34.0	35.0	27.0	36.0
92-93	32.10054046185138	35.0	33.5	35.0	27.0	36.0
94-95	31.964648400784245	35.0	33.0	35.0	27.0	35.5
96-97	31.80935770478594	35.0	33.0	35.0	27.0	35.0
98-99	31.634602614964294	35.0	33.0	35.0	26.0	35.0
100	31.47781483201343	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1972186992545346
1101	2	0.16210148537127367
1101	3	0.20484596687880696
1101	4	0.15447006672443564
1101	5	0.12194522573931721
1101	6	0.16363733555710525
1101	7	0.18015290911232995
1101	8	0.16117094557338874
1101	9	0.2574560413942706
1101	10-11	0.24465945814969814
1101	12-13	0.26302407374463144
1101	14-15	0.26651165277552025
1101	16-17	0.21306281982499087
1101	18-19	0.2372679032202072
1101	20-21	0.23325471932253095
1101	22-23	0.2284444088500095
1101	24-25	0.25480784719076155
1101	26-27	0.22933453233116552
1101	28-29	0.2839480728310235
1101	30-31	0.2931538990111875
1101	32-33	0.3016123910312416
1101	34-35	0.3422344756342852
1101	36-37	0.2911245805637961
1101	38-39	0.28783979443768004
1101	40-41	0.2675508087598999
1101	42-43	0.22817906337434835
1101	44-45	0.24689855989566212
1101	46-47	0.2589109479937477
1101	48-49	0.26260213646774844
1101	50-51	0.30604961042879353
1101	52-53	0.26415264926237114
1101	54-55	0.23905534351828805
1101	56-57	0.2881856554457656
1101	58-59	0.3224588618801718
1101	60-61	0.30141756135088826
1101	62-63	0.29874673362826343
1101	64-65	0.2769024722408062
1101	66-67	0.2529827009504544
1101	68-69	0.23738014128764462
1101	70-71	0.33396331858383377
1101	72-73	0.294700168892021
1101	74-75	0.2581267249891255
1101	76-77	0.2694065955751199
1101	78-79	0.27152615800773106
1101	80-81	0.24885567531894282
1101	82-83	0.25414936714749103
1101	84-85	0.15333653393192748
1101	86-87	0.25615969160666907
1101	88-89	0.39182980955026636
1101	90-91	0.4002720900972534
1101	92-93	0.3790711930764772
1101	94-95	0.3514411506028132
1101	96-97	0.371809935606084
1101	98-99	0.4778505803651605
1101	100	0.5038219556297499
1102	1	0.12128967179994632
1102	2	0.13536432912541585
1102	3	0.19005950480932654
1102	4	0.12291380801072904
1102	5	0.06607760340118318
1102	6	0.12327555594609407
1102	7	0.14557766852809095
1102	8	0.14628663339383507
1102	9	0.17101452476083523
1102	10-11	0.15379198570828834
1102	12-13	0.11448995554997765
1102	14-15	0.18875485882232113
1102	16-17	0.15661687428416826
1102	18-19	0.18802821959192784
1102	20-21	0.19354069445945754
1102	22-23	0.19521857876983972
1102	24-25	0.21905896160338045
1102	26-27	0.2659637289372512
1102	28-29	0.2406143356522108
1102	30-31	0.2333078784911038
1102	32-33	0.28773981993769837
1102	34-35	0.29685976318101837
1102	36-37	0.33387588396991674
1102	38-39	0.2711771198659392
1102	40-41	0.3419743785828757
1102	42-43	0.271362100257285
1102	44-45	0.24625282952618477
1102	46-47	0.2856871855611942
1102	48-49	0.2413414030122496
1102	50-51	0.2789414675132349
1102	52-53	0.2579105323620894
1102	54-55	0.2548308149125873
1102	56-57	0.18775313385213366
1102	58-59	0.1584600066584656
1102	60-61	0.07356129469420125
1102	62-63	0.21603631648576282
1102	64-65	0.2234810099349005
1102	66-67	0.18492377701114293
1102	68-69	0.18634681198246028
1102	70-71	0.1648985876144593
1102	72-73	0.1920260576637034
1102	74-75	0.13844615027581142
1102	76-77	0.13197152635528653
1102	78-79	0.12588656104139062
1102	80-81	0.14433353835412532
1102	82-83	0.11350822330875587
1102	84-85	0.1761611847420923
1102	86-87	0.1464795001344683
1102	88-89	0.2262664961094103
1102	90-91	0.27838043050560657
1102	92-93	0.2939367449853947
1102	94-95	0.29528819907446646
1102	96-97	0.307061967633917
1102	98-99	0.2908940062192862
1102	100	0.2516433998862482
1103	1	-0.08208592509017265
1103	2	-0.11849076928346491
1103	3	-0.0965083430388951
1103	4	-0.09211087943337049
1103	5	-0.09791250822939901
1103	6	-0.04354486320523421
1103	7	-0.08473382213178837
1103	8	-0.10266252127104991
1103	9	-0.11506479104670575
1103	10-11	-0.06430566826360007
1103	12-13	-0.07297225173812905
1103	14-15	-0.048220454685889536
1103	16-17	-0.06934394036323965
1103	18-19	-0.09436137881812812
1103	20-21	-0.0762703942834122
1103	22-23	-0.06368129147573853
1103	24-25	-0.062116895378871106
1103	26-27	-0.024803780313263246
1103	28-29	0.020352510928503875
1103	30-31	0.012488509433687511
1103	32-33	-0.05341658801738447
1103	34-35	-0.016070036615772665
1103	36-37	0.026560691019255955
1103	38-39	-0.026384469588052184
1103	40-41	0.027698184362940026
1103	42-43	-0.05639375121290158
1103	44-45	-0.17981714893894463
1103	46-47	-0.1363737708932362
1103	48-49	-0.11631632333356379
1103	50-51	-0.14377422781953442
1103	52-53	-0.2157355507557952
1103	54-55	-0.1806908122276596
1103	56-57	-0.2391083432984118
1103	58-59	-0.19275283752025985
1103	60-61	-0.18108909673703977
1103	62-63	-0.1364243139949508
1103	64-65	-0.12864241559011447
1103	66-67	-0.1802019930569756
1103	68-69	-0.151720871786722
1103	70-71	-0.12373864696088788
1103	72-73	-0.09012466119649787
1103	74-75	-0.08412202123679435
1103	76-77	-0.10775758298527194
1103	78-79	-0.10890342270664632
1103	80-81	-0.04006881146832342
1103	82-83	-0.024215211429790884
1103	84-85	-0.01840308387089351
1103	86-87	-0.035989627577826866
1103	88-89	-0.004301952999860248
1103	90-91	0.05820907467830949
1103	92-93	0.03379136503999547
1103	94-95	0.01776460715216288
1103	96-97	0.014154645786735642
1103	98-99	0.1072224159317301
1103	100	0.13431604392813412
1104	1	0.11298349574521538
1104	2	0.05260204000696689
1104	3	0.1390908568580116
1104	4	0.09502029974856896
1104	5	0.036373170715421566
1104	6	0.06826343336938834
1104	7	-0.06736956483284473
1104	8	-0.001408990053860748
1104	9	-0.022152975299981392
1104	10-11	-0.01008320364476134
1104	12-13	0.031139418981986466
1104	14-15	-0.03204473686800213
1104	16-17	0.03489265183816315
1104	18-19	0.005780556894642075
1104	20-21	-2.967944516854004E-4
1104	22-23	0.0437754354289126
1104	24-25	0.06354124557523733
1104	26-27	0.08990187762429969
1104	28-29	0.1277714871499711
1104	30-31	0.13661269858314995
1104	32-33	0.11570380163936989
1104	34-35	0.07003810048042425
1104	36-37	0.13270791342739585
1104	38-39	0.06698098282067377
1104	40-41	0.1540881286664728
1104	42-43	0.1117776151210137
1104	44-45	0.054611808346052726
1104	46-47	0.033407748491917744
1104	48-49	-0.021685044668650733
1104	50-51	-0.0037351333663053765
1104	52-53	-0.005206346738731327
1104	54-55	-0.0012720561970809285
1104	56-57	0.014768043396443886
1104	58-59	-0.025421670273416908
1104	60-61	0.02698404183361447
1104	62-63	-5.345095389159837E-4
1104	64-65	0.023200801866195775
1104	66-67	-0.010789753339942365
1104	68-69	-0.054931990889805604
1104	70-71	-0.033830088742462294
1104	72-73	-0.004928110971903266
1104	74-75	-0.05272716477379902
1104	76-77	-0.12860483000920198
1104	78-79	-0.08596593335303737
1104	80-81	0.015891698603262228
1104	82-83	0.041427813253783086
1104	84-85	-0.003641966851738232
1104	86-87	0.07446467211357799
1104	88-89	0.16945658586045198
1104	90-91	0.12123651835373295
1104	92-93	0.05372159754963235
1104	94-95	0.10512065928370262
1104	96-97	0.10355271992770909
1104	98-99	0.11203536379616352
1104	100	0.11484177625353453
1105	1	0.15679557620400786
1105	2	0.15072102496088746
1105	3	0.1290988480930224
1105	4	0.10427306162239347
1105	5	0.09832528326433732
1105	6	0.15067289340152
1105	7	0.09343311996735082
1105	8	0.12992712752809865
1105	9	0.14691550937539688
1105	10-11	0.13778915336983744
1105	12-13	0.16218087555890293
1105	14-15	0.17173003999622694
1105	16-17	0.18092407077819672
1105	18-19	0.1715243111410487
1105	20-21	0.19088160193575732
1105	22-23	0.20282968753573982
1105	24-25	0.1821845788679468
1105	26-27	0.22915629935131676
1105	28-29	0.2691506774556842
1105	30-31	0.19155558272166218
1105	32-33	0.19498276646201163
1105	34-35	0.22200755775399728
1105	36-37	0.20458127272318194
1105	38-39	0.17425657291962438
1105	40-41	0.20715260037358263
1105	42-43	0.21519278366447026
1105	44-45	0.19512790793373114
1105	46-47	0.24903552715370836
1105	48-49	0.24236173068628375
1105	50-51	0.23305390670485338
1105	52-53	0.16225929243323378
1105	54-55	0.13968075005296754
1105	56-57	0.2321934655828244
1105	58-59	0.2167596689354525
1105	60-61	0.1725585618845713
1105	62-63	0.20472768357355875
1105	64-65	0.23027468109191318
1105	66-67	0.24123038903076832
1105	68-69	0.16497641629135273
1105	70-71	0.21406186200728428
1105	72-73	0.21037372314665959
1105	74-75	0.19775169710828777
1105	76-77	0.2043760521937088
1105	78-79	0.1950929371319603
1105	80-81	0.1736069082065299
1105	82-83	0.1526105786296057
1105	84-85	0.14199694594935153
1105	86-87	0.14878507945682173
1105	88-89	0.17945261659854594
1105	90-91	0.18386986803852068
1105	92-93	0.15505426254909338
1105	94-95	0.2433752981149091
1105	96-97	0.2932363424642439
1105	98-99	0.3166325586607357
1105	100	0.2945766708481905
1106	1	0.0840498573056685
1106	2	0.03281056640772917
1106	3	0.06505905412998203
1106	4	0.09027369624003256
1106	5	0.04309059944036164
1106	6	0.04932390127618902
1106	7	0.0031092470057316746
1106	8	0.07313042083315224
1106	9	0.050714978585226334
1106	10-11	0.08415961414038975
1106	12-13	0.0389683836514223
1106	14-15	0.11847133187066561
1106	16-17	0.04225100009021787
1106	18-19	0.0938760766795923
1106	20-21	0.07318043830940724
1106	22-23	0.02961603768161325
1106	24-25	0.05821090732616341
1106	26-27	0.14284293006609516
1106	28-29	0.19016173988063656
1106	30-31	0.1641542947915724
1106	32-33	0.15350443641179368
1106	34-35	0.10439373788607043
1106	36-37	0.11342878676367718
1106	38-39	0.09903565137014425
1106	40-41	0.06964504752247791
1106	42-43	0.08834931688328851
1106	44-45	0.08024498210330222
1106	46-47	0.0864936131527827
1106	48-49	0.11786845023495829
1106	50-51	0.10815641904038387
1106	52-53	0.05544502791378392
1106	54-55	0.10279648163318456
1106	56-57	0.1317510251720222
1106	58-59	0.10738673529386489
1106	60-61	0.1021573705722929
1106	62-63	0.12956953216359324
1106	64-65	0.1427809659652297
1106	66-67	0.16209586979240242
1106	68-69	0.1085315734294241
1106	70-71	0.13798620816563556
1106	72-73	0.23252338245885085
1106	74-75	0.18071397927547395
1106	76-77	0.16696300560359845
1106	78-79	0.20444078891563322
1106	80-81	0.17222135430878183
1106	82-83	0.1231890929662427
1106	84-85	0.150614793585369
1106	86-87	0.1858997844989858
1106	88-89	0.30036281191532765
1106	90-91	0.2738933560169343
1106	92-93	0.2761898382958847
1106	94-95	0.23077099646362242
1106	96-97	0.29559234472522533
1106	98-99	0.2564232626860097
1106	100	0.27470384377960855
1107	1	0.1622619054809178
1107	2	0.09604522695472184
1107	3	0.08503578513267485
1107	4	0.04814881307929397
1107	5	0.03498243157110892
1107	6	0.07525971472022519
1107	7	0.05369068060285542
1107	8	0.057153478276013914
1107	9	0.1113838940305314
1107	10-11	0.10444359212299048
1107	12-13	0.09023146779357205
1107	14-15	0.07978140719610849
1107	16-17	0.05885223494468761
1107	18-19	0.11836829744702015
1107	20-21	0.037246560628418024
1107	22-23	0.06104727424376222
1107	24-25	0.05515012830080934
1107	26-27	0.12169732089411411
1107	28-29	0.14795762340357044
1107	30-31	0.11477706068968985
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2216	18-19	-0.18588131692945353
2216	20-21	-0.22201969640566688
2216	22-23	-0.18474387953887828
2216	24-25	-0.15901673542057893
2216	26-27	-0.3009875433035347
2216	28-29	-0.20793930015312156
2216	30-31	-0.21324369468906923
2216	32-33	-0.1534231261663308
2216	34-35	-0.18773025806923727
2216	36-37	-0.19984472044382784
2216	38-39	-0.17419862100661732
2216	40-41	-0.18641313471818677
2216	42-43	-0.184730476156588
2216	44-45	-0.1191931966696913
2216	46-47	-0.05417799712311222
2216	48-49	-0.04866228299909636
2216	50-51	-0.0828993490549692
2216	52-53	-0.11531695034016565
2216	54-55	-0.07956260282684724
2216	56-57	-0.12871730279118765
2216	58-59	-0.13243730349213223
2216	60-61	-0.0701713478260757
2216	62-63	-0.02818810939128724
2216	64-65	-0.03676641606841713
2216	66-67	-0.054970072682060334
2216	68-69	-0.06588679442335632
2216	70-71	-0.18034866766226543
2216	72-73	-0.02332214729128168
2216	74-75	-0.016360702153491502
2216	76-77	-0.049834240996950996
2216	78-79	-0.12173784826344303
2216	80-81	-0.016955388470059063
2216	82-83	-0.0370086973788446
2216	84-85	-0.05829487924702903
2216	86-87	-0.07416003324298259
2216	88-89	-0.06355507047154418
2216	90-91	-0.04718178365745729
2216	92-93	-0.01532177771456844
2216	94-95	0.023845194495589084
2216	96-97	-0.040133951154793124
2216	98-99	-0.033670210294264535
2216	100	-0.016430600057862677
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32062.0
3	5219.0
4	3560.0
5	3113.0
6	3270.0
7	4383.0
8	4877.0
9	4699.0
10	4949.0
11	5500.0
12	5852.0
13	6415.0
14	7025.0
15	7093.0
16	7145.0
17	7409.0
18	7340.0
19	7059.0
20	7413.0
21	7622.0
22	9022.0
23	11921.0
24	17635.0
25	23508.0
26	24608.0
27	23468.0
28	26841.0
29	34209.0
30	42984.0
31	54903.0
32	74031.0
33	102987.0
34	148587.0
35	242297.0
36	483519.0
37	979577.0
38	1331986.0
39	422794.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11351159340678	18.364072032636233	12.834811343892397	24.687605030064592
2	15.92535122066999	20.009350776985162	38.8329419285739	25.23235607377095
3	18.941169935268498	24.565069212471954	29.287386071390852	27.206374780868696
4	12.99037805388757	16.36884452509145	36.72123051459976	33.91954690642122
5	14.119799024775807	37.36967394561064	34.33836041534625	14.172166614267296
6	32.50587183214745	36.10738929895674	17.668871319129103	13.71786754976671
7	28.721418472026905	31.73751100228886	21.570943378063664	17.970127147620573
8	26.52187174580782	34.94460873440073	19.86553111990678	18.667988399884667
9	26.370115513352165	16.181591927171826	19.58277074767455	37.86552181180146
10-11	25.243877474908054	25.797333677766453	27.819738353160083	21.13905049416541
12-13	25.729179077802932	23.09627070177529	27.817788633384964	23.356761587036818
14-15	23.046986936442785	24.641961257692216	24.80793810171819	27.503113704146813
16-17	23.42173866263521	28.053614410386018	25.259437730714744	23.265209196264024
18-19	23.41816607291732	27.26599307422834	26.625806607662287	22.69003424519205
20-21	24.345987966371617	26.444324361037445	26.150662838561235	23.059024834029703
22-23	23.9985201962825	26.271317603170736	26.04119740188407	23.688964798662695
24-25	23.898286059911047	26.297918958962335	26.215819324561902	23.587975656564716
26-27	23.694190971040644	26.47418098144012	26.076276986457803	23.755351061061432
28-29	23.565228477503744	26.615523196862046	26.14280454631568	23.67644377931853
30-31	23.83422683350632	26.72201589748362	25.84624949762584	23.59750777138422
32-33	23.7054589486764	26.547108851765007	26.148429220364335	23.599002979194257
34-35	23.693086616182768	26.434322537998455	26.27029375176262	23.60229709405616
36-37	23.95287920689029	26.465647432558466	25.834861104293662	23.746612256257585
38-39	23.81123267792103	26.688756003378927	25.88492575528494	23.615085563415104
40-41	23.74108819218302	26.538550847507103	26.124146795037444	23.596214165272432
42-43	23.744307850597206	26.5396115935302	25.98522346489185	23.73085709098075
44-45	23.669032494882668	26.516628210491	26.06375595890028	23.75058333572605
46-47	23.737402017678694	26.52752583335458	26.09705530614613	23.638016842820594
48-49	23.78792481692084	26.469833486420512	25.951207482567717	23.79103421409093
50-51	23.58953219899592	26.584573741860005	26.083676644737626	23.74221741440645
52-53	23.804741302538755	26.365916757657693	26.059734193027257	23.7696077467763
54-55	23.693362730490282	26.502008754100693	26.018735505287165	23.78589301012186
56-57	23.474019561007886	26.501808543817845	26.095949438447203	23.928222456727067
58-59	23.537093697697184	26.25082097067355	26.255860268259802	23.956225063369466
60-61	23.575589121678764	26.51467200477866	26.111371019632045	23.798367853910523
62-63	23.546348825769865	26.430879099762656	26.104671962400044	23.918100112067435
64-65	23.637536105742917	26.423185158517406	26.024595388138373	23.9146833476013
66-67	23.47911415492258	26.343381358026075	26.03135467850602	24.146149808545328
68-69	23.571259573204774	26.321699708911193	26.151161269678042	23.955879448205994
70-71	23.45549703291315	26.326528229616734	26.170037211886648	24.04793752558347
72-73	23.59291405586684	26.290260382869523	26.150388979739315	23.966436581524324
74-75	23.59272517254985	26.40042477474644	26.031796634205755	23.975053418497954
76-77	23.607604459821093	26.24870308670428	26.121314005329303	24.02237844814533
78-79	23.698027081451286	26.237611554237894	26.18730187511072	23.877059489200096
80-81	23.644393190842496	26.113671805660953	26.346874370829603	23.895060632666944
82-83	23.612794670154198	26.069809362278622	26.343609175456685	23.973786792110495
84-85	23.652356154170835	26.096924257797703	26.123395423823975	24.12732416420748
86-87	23.579048893412704	26.224527334450308	26.268272366672957	23.928151405464032
88-89	23.488740298624805	26.083463795574545	26.24662199371073	24.18117391208992
90-91	23.59198677727158	26.01676093493991	26.20143805561648	24.18981423217203
92-93	23.645663227851703	26.030556812359357	26.28262204896641	24.041157910822527
94-95	23.501971749698818	25.94751180371447	26.339752599849074	24.210763846737642
96-97	23.54966604891558	25.798136201232268	26.451502345476847	24.20069540437531
98-99	23.404204836687413	26.006850322150644	26.405759418593792	24.183185422568148
100	23.6908108403482	25.78667827935105	26.153369400081345	24.36914148021941
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	578.0
1	492.0
2	427.0
3	419.5
4	346.5
5	264.5
6	220.0
7	237.0
8	251.0
9	236.5
10	222.5
11	221.0
12	232.0
13	232.0
14	255.5
15	302.5
16	349.5
17	417.5
18	534.0
19	688.5
20	891.0
21	1313.5
22	2009.5
23	2945.0
24	4370.5
25	6417.0
26	9466.5
27	13776.5
28	19757.5
29	27797.5
30	36145.5
31	43967.0
32	52533.0
33	62704.0
34	72145.5
35	80483.5
36	90562.5
37	102511.5
38	113290.0
39	121234.0
40	129715.5
41	135427.0
42	137341.0
43	142757.0
44	148184.0
45	149914.5
46	148495.0
47	146676.0
48	149234.5
49	152531.5
50	152737.0
51	150486.5
52	148049.5
53	148566.5
54	147006.0
55	142562.0
56	133103.0
57	120822.5
58	109819.5
59	96906.0
60	85820.5
61	77613.5
62	69107.0
63	58905.5
64	47914.5
65	39562.0
66	33495.0
67	27432.5
68	22939.5
69	19094.0
70	14808.0
71	10483.0
72	7808.0
73	6338.0
74	4866.0
75	3302.5
76	2102.5
77	1356.0
78	907.0
79	606.0
80	435.0
81	290.5
82	186.5
83	122.5
84	73.5
85	59.0
86	51.0
87	37.0
88	25.0
89	21.5
90	13.5
91	10.0
92	13.0
93	10.5
94	8.0
95	8.0
96	9.0
97	4.5
98	4.0
99	5.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3293901029531699
2	0.11879486823323726
3	0.06547298393600802
4	0.0390264729574895
5	0.04307683950374909
6	0.01000678793781782
7	0.007076228613171172
8	0.015272264447955292
9	0.01794074123137338
10-11	0.021061906040549888
12-13	0.015510521303617622
14-15	0.01864359895557725
16-17	0.019453672264829167
18-19	0.018953332867938277
20-21	0.02367081861005239
22-23	0.015450957089702039
24-25	0.02454045613321989
26-27	0.018750814540625296
28-29	0.018726988855059065
30-31	0.019691929120491496
32-33	0.02501696984454455
34-35	0.015045920435076078
36-37	0.009649402654324325
38-39	0.013247081174825493
40-41	0.009566012754842512
42-43	0.008624898174976312
44-45	0.009113324729084086
46-47	0.00829133857704905
48-49	0.004669834370981649
50-51	0.006695017644111446
52-53	0.007350223997182851
54-55	0.003490462935453121
56-57	0.0030496877524778115
58-59	0.0032760317653570243
60-61	0.0038478482189466135
62-63	0.002966297852995997
64-65	0.002477871298888222
66-67	0.0017631007319012348
68-69	0.003573852834934935
70-71	0.002501696984454455
72-73	0.0019417933736479815
74-75	0.0024540456133219893
76-77	0.0039669766467777786
78-79	0.003466637249886888
80-81	0.0037168069483323333
82-83	0.002537435512803804
84-85	0.003133077651959627
86-87	0.0024540456133219893
88-89	0.598656088379951
90-91	0.6086509634749858
92-93	0.60549406013746
94-95	0.5918657679935746
96-97	0.5898882360915774
98-99	0.5872197593081593
100	0.5885420848570853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4197151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.6152124660278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.56267218249698	19.147017485600333
2	14.146889087752246	8.94513808485235
3	6.841457735881362	6.488824196917163
4	4.118558952995457	5.208364654112494
5	2.7450955764037874	4.33933899437794
6	2.0086187561809785	3.8101745243946072
7	1.5418407001428507	3.4121934926649695
8	1.1597849806186198	2.9333478861732476
9	0.901439877263561	2.5649291920533748
>10	5.603393538202699	31.76938104654843
>50	0.2764882977643869	5.9034122364280055
>100	0.09096084667481402	4.815926868882107
>500	0.0022698386501012226	0.4587469021121297
>1k	5.296289720661816E-4	0.20320443488272547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	7.147705669869871E-5	0.0	0.0	0.0
6	0.0	7.147705669869871E-5	0.0	0.0	0.0
7	0.0	7.147705669869871E-5	0.0	0.0	0.0
8	0.0	7.147705669869871E-5	0.0	0.0	0.0
9	0.0	7.147705669869871E-5	0.0	0.0	0.0
10-11	0.0	7.147705669869871E-5	0.0	0.0	0.0
12-13	0.0	7.147705669869871E-5	0.0	2.3825685566232903E-5	0.0
14-15	0.0	7.147705669869871E-5	0.0	2.3825685566232903E-5	0.0
16-17	0.0	9.530274226493161E-5	0.0	1.1912842783116453E-4	0.0
18-19	0.0	1.1912842783116453E-4	0.0	1.1912842783116453E-4	0.0
20-21	0.0	1.1912842783116453E-4	0.0	1.3104127061428096E-4	0.0
22-23	0.0	1.5486695618051387E-4	0.0	2.0251832731297967E-4	0.0
24-25	0.0	1.9060548452986322E-4	0.0	4.2886234019219225E-4	0.0
26-27	0.0	1.9060548452986322E-4	0.0	8.338989948181517E-4	0.0
28-29	0.0	2.0251832731297967E-4	0.0	0.0025136098272375714	0.0
30-31	0.0	2.1443117009609612E-4	0.0	0.009458797169794463	0.0
32-33	0.0	2.1443117009609612E-4	0.0	0.025171836800725066	0.0
34-35	0.0	2.1443117009609612E-4	0.0	0.04737737574845413	0.0
36-37	0.0	2.1443117009609612E-4	0.0	0.08641576154872674	0.0
38-39	0.0	2.3825685566232905E-4	0.0	0.14713552121427131	0.0
40-41	0.0	2.3825685566232905E-4	0.0	0.2168137386527194	0.0
42-43	0.0	2.3825685566232905E-4	0.0	0.28938677688746484	0.0
44-45	0.0	2.501696984454455E-4	0.0	0.3734080570367852	0.0
46-47	0.0	2.6208254122856193E-4	0.0	0.474154968453601	0.0
48-49	0.0	2.978210695779113E-4	0.0	0.5821329754397686	0.0
50-51	0.0	3.0973391236102773E-4	0.0	0.6948999452247488	0.0
52-53	0.0	3.0973391236102773E-4	0.0	0.8109667724606524	0.0
54-55	0.0	3.0973391236102773E-4	0.0	0.9400066854873699	0.0
56-57	0.0	3.454724407103771E-4	0.0	1.0890959129180722	0.0
58-59	0.0	4.0503665462595935E-4	0.0	1.2560782302090154	0.0
60-61	0.0	4.0503665462595935E-4	0.0	1.4284570652807105	0.0
62-63	0.0	4.169494974090758E-4	0.0	1.6058512071640978	0.0
64-65	0.0	4.2886234019219225E-4	0.0	1.7987439575083193	0.0
66-67	0.0	5.479907680233568E-4	0.0	2.0145927558956065	0.0
68-69	0.0	7.266834097701036E-4	0.0	2.254648450818186	0.0
70-71	0.0	7.3859625255322E-4	0.0	2.510703093598491	0.0
72-73	0.0	7.3859625255322E-4	0.0	2.774512997030605	0.0
74-75	0.0	7.981604664688022E-4	0.0	3.051045816555087	0.0
76-77	0.0	8.100733092519187E-4	0.0	3.356610233942024	0.0
78-79	0.0	8.100733092519187E-4	0.0	3.6990568125854892	0.0
80-81	0.0	8.45811837601268E-4	0.0	4.053964224780095	0.0
82-83	0.0	8.577246803843845E-4	0.0	4.427312717602964	0.0
84-85	0.0	8.577246803843845E-4	0.0	4.808106737165282	0.0
86-87	0.0	9.292017370830833E-4	0.0	5.22055318000234	0.0
88	0.0	9.292017370830833E-4	0.0	5.550241104025088	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	14420	0.0	28.256401	1
GTATCAA	7810	0.0	27.925179	1
TACATGG	14750	0.0	26.77687	2
TCAACGC	7920	0.0	26.397982	4
CAACGCA	8135	0.0	25.700615	5
ACATGGG	14820	0.0	25.39851	3
AACGCAG	8330	0.0	25.257648	6
ATCAACG	8320	0.0	25.190424	3
TATCAAC	8755	0.0	24.328459	2
ACGCAGA	9750	0.0	21.145592	7
CATGGGG	12065	0.0	21.02821	4
GGTATCA	6065	0.0	20.42636	1
CGCAGAG	10270	0.0	19.846283	8
GAGTACA	9705	0.0	19.754448	1
TATACTG	1575	0.0	19.09152	5
GTATAGG	1220	0.0	18.919178	1
AGTACAT	10040	0.0	18.217485	2
GCAGAGT	11440	0.0	17.15972	9
GTATATA	1820	0.0	17.081993	1
GTATAGA	1520	0.0	16.424732	1
>>END_MODULE