FastQCFastQC Report
Wed 25 May 2016
SRR1033195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197151
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50640.12065327170740342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42930.10228366813583786No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110600.037.269021
GGTATCA74300.034.1982541
TCAACGC126850.031.7656654
CAACGCA129000.031.236615
ATCAACG130850.030.8664763
AACGCAG132750.030.3061566
TATCAAC135300.030.0948962
GTACATG140300.027.7361931
TACATGG143400.026.2309252
ACGCAGA152700.025.9773647
CGCAGAG158150.025.050058
ACATGGG145100.024.8539563
GCAGAGT174400.022.3117759
GAGTACT81950.021.27502312-13
TATACTG16450.021.1513755
CATGGGG115650.020.8157424
AGAGTAC146600.019.18582210-11
GAGTACA95500.018.7231641
GTACTTT92150.018.63962214-15
AGTACTT89100.018.59201612-13
Produced by FastQC (version 0.11.5)