##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033195_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4197151
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73843495266193	34.0	31.0	34.0	31.0	34.0
2	32.83074733313145	34.0	31.0	34.0	31.0	34.0
3	32.92879812996959	34.0	31.0	34.0	31.0	34.0
4	36.35326939631193	37.0	37.0	37.0	35.0	37.0
5	36.29111556863215	37.0	37.0	37.0	35.0	37.0
6	36.364904193344486	37.0	37.0	37.0	35.0	37.0
7	36.33613563105068	37.0	37.0	37.0	35.0	37.0
8	36.33644822404531	37.0	37.0	37.0	35.0	37.0
9	38.15787066035985	39.0	39.0	39.0	37.0	39.0
10-11	38.1304189437073	39.0	39.0	39.0	37.0	39.0
12-13	38.09406666569775	39.0	39.0	39.0	37.0	39.0
14-15	39.624557348544286	41.0	40.0	41.0	37.0	41.0
16-17	39.63137637888177	41.0	40.0	41.0	37.0	41.0
18-19	39.62984879505169	41.0	40.0	41.0	37.0	41.0
20-21	39.615332876992035	41.0	40.0	41.0	37.0	41.0
22-23	39.54579058508974	41.0	40.0	41.0	37.0	41.0
24-25	39.49174571036401	41.0	39.0	41.0	36.5	41.0
26-27	39.38884031096332	41.0	39.0	41.0	36.0	41.0
28-29	39.30153978258109	41.0	39.0	41.0	36.0	41.0
30-31	39.22276420362289	41.0	39.0	41.0	36.0	41.0
32-33	39.28019518478129	41.0	39.0	41.0	35.5	41.0
34-35	39.224608788199426	41.0	39.0	41.0	35.0	41.0
36-37	39.14679338436954	41.0	39.0	41.0	35.0	41.0
38-39	39.05696614203301	41.0	39.0	41.0	35.0	41.0
40-41	38.944193096698214	41.0	39.0	41.0	35.0	41.0
42-43	38.82708508700307	41.0	39.0	41.0	35.0	41.0
44-45	38.687950111873505	41.0	38.0	41.0	35.0	41.0
46-47	38.491504713554505	41.0	38.0	41.0	35.0	41.0
48-49	38.314383852284564	40.0	38.0	41.0	34.5	41.0
50-51	38.1690530076235	40.0	38.0	41.0	34.0	41.0
52-53	38.011622407676064	40.0	37.0	41.0	34.0	41.0
54-55	37.846011496846316	40.0	37.0	41.0	34.0	41.0
56-57	37.65017841864636	40.0	36.5	41.0	33.5	41.0
58-59	37.427707985726514	39.5	36.0	41.0	33.0	41.0
60-61	37.20032338603019	39.0	35.0	41.0	33.0	41.0
62-63	36.9296264299283	39.0	35.0	41.0	33.0	41.0
64-65	36.64913961875567	38.5	35.0	41.0	33.0	41.0
66-67	36.33550401212632	37.5	35.0	40.0	32.0	41.0
68-69	35.988824085671446	37.0	35.0	40.0	32.0	41.0
70-71	35.6397451509369	36.5	35.0	39.0	32.0	41.0
72-73	35.28210719604799	36.0	35.0	39.0	31.0	41.0
74-75	34.950100675434356	35.5	35.0	38.5	31.0	40.5
76-77	34.62906755082197	35.0	35.0	37.0	31.0	39.0
78-79	34.31941142932432	35.0	35.0	37.0	31.0	39.0
80-81	34.01739834949946	35.0	34.0	36.5	31.0	39.0
82-83	33.747610819815634	35.0	34.0	36.0	30.5	37.0
84-85	33.52082507872602	35.0	34.0	36.0	30.0	37.0
86-87	33.33717788566578	35.0	34.0	35.0	30.0	37.0
88-89	33.1640595013141	35.0	34.0	35.0	30.0	36.0
90-91	32.99964714159677	35.0	34.0	35.0	30.0	36.0
92-93	32.865704974636365	35.0	34.0	35.0	30.0	36.0
94-95	32.74312861271848	35.0	34.0	35.0	29.5	35.5
96-97	32.63750899121809	35.0	34.0	35.0	29.5	35.0
98-99	32.5479429975238	35.0	34.0	35.0	29.0	35.0
100	32.45705098529931	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.05232242209666538
1101	2	0.09402472512478965
1101	3	0.1394302894225632
1101	4	0.050331503476954254
1101	5	0.07848585956766385
1101	6	0.08978498678625613
1101	7	0.09051040190683324
1101	8	0.1026963610311995
1101	9	0.16537100219910883
1101	10-11	0.1402051529620536
1101	12-13	0.15343272501496585
1101	14-15	0.13440967757751565
1101	16-17	0.16093406373374108
1101	18-19	0.16331871210927318
1101	20-21	0.18720121748197727
1101	22-23	0.22663374927959268
1101	24-25	0.1431558618913229
1101	26-27	0.14474541353048664
1101	28-29	0.19204291002307627
1101	30-31	0.14998222762456948
1101	32-33	0.17006368755214396
1101	34-35	0.19286601785150026
1101	36-37	0.20944891053571268
1101	38-39	0.21081436517887653
1101	40-41	0.2642884097445304
1101	42-43	0.21789397268481991
1101	44-45	0.15521760138275198
1101	46-47	0.22784000499650858
1101	48-49	0.18120022256132984
1101	50-51	0.2267158002509504
1101	52-53	0.2971174098531151
1101	54-55	0.3115590641676391
1101	56-57	0.30908803550141073
1101	58-59	0.30418661988750273
1101	60-61	0.29148700623362345
1101	62-63	0.2766847426319785
1101	64-65	0.310293958180587
1101	66-67	0.29085061420808955
1101	68-69	0.31262741187978804
1101	70-71	0.2692768410725819
1101	72-73	0.32234411515567274
1101	74-75	0.2890025386871997
1101	76-77	0.21904957042728057
1101	78-79	0.2253755116695686
1101	80-81	0.21457178949416544
1101	82-83	0.2409410616175265
1101	84-85	0.23566976989161503
1101	86-87	0.2078386783214654
1101	88-89	0.1860102843050413
1101	90-91	0.24630799446821072
1101	92-93	0.18048109734565543
1101	94-95	0.20959311645994916
1101	96-97	0.2561232073392006
1101	98-99	0.25110669857706114
1101	100	0.2509994182625306
1102	1	0.08140365406849526
1102	2	0.09766492847747088
1102	3	0.08798010814024337
1102	4	0.05998800508517377
1102	5	0.02079030074494881
1102	6	0.04746394879624205
1102	7	0.08427599618863013
1102	8	0.10294030636246987
1102	9	0.15191364503600369
1102	10-11	0.0758436558368274
1102	12-13	0.12351726367765536
1102	14-15	0.16221351128672978
1102	16-17	0.13597669158096437
1102	18-19	0.15673884852456865
1102	20-21	0.1783016634324639
1102	22-23	0.18770436423631764
1102	24-25	0.14754650697574334
1102	26-27	0.146575266220637
1102	28-29	0.10424980397438333
1102	30-31	0.10411256236404398
1102	32-33	0.11869126712798561
1102	34-35	0.1071297266797373
1102	36-37	0.0812418082743136
1102	38-39	0.11479545222992726
1102	40-41	0.06865831653967547
1102	42-43	0.0353278581841181
1102	44-45	0.0749662013936927
1102	46-47	0.11067139034204843
1102	48-49	0.09472320976509963
1102	50-51	0.06893765914960426
1102	52-53	0.06471261204525547
1102	54-55	0.061340446747045974
1102	56-57	0.0706008921590282
1102	58-59	0.09428611113492025
1102	60-61	0.06952631688175614
1102	62-63	0.0356595386565246
1102	64-65	-0.013299570113268544
1102	66-67	0.012720299248798028
1102	68-69	0.028319791037532127
1102	70-71	0.018595336381437733
1102	72-73	0.04138504808817345
1102	74-75	0.08427311154922279
1102	76-77	-0.021216429811936166
1102	78-79	0.02263717664822451
1102	80-81	-0.002697410499280295
1102	82-83	-0.03003352362441092
1102	84-85	-3.711370601422459E-5
1102	86-87	0.05936762492454761
1102	88-89	0.07080805198737039
1102	90-91	0.05017432553104584
1102	92-93	0.049808116678619285
1102	94-95	-0.018668226781606734
1102	96-97	-0.0411135778736309
1102	98-99	-0.014562407955011736
1102	100	-0.029272689109269834
1103	1	0.04084626924574053
1103	2	0.062014290905359815
1103	3	0.07649865288019697
1103	4	0.038708351709608735
1103	5	-8.156358478430548E-4
1103	6	0.045392984815329385
1103	7	0.06911010584330057
1103	8	0.05608209025852773
1103	9	0.05814287197124912
1103	10-11	0.06924223867132184
1103	12-13	0.07571291849822614
1103	14-15	0.11016776699867137
1103	16-17	0.1126927801558466
1103	18-19	0.08913026672837532
1103	20-21	0.0535734458354824
1103	22-23	0.07459318620600186
1103	24-25	0.07382462342165752
1103	26-27	0.13926814702379176
1103	28-29	0.06355025799433633
1103	30-31	0.09414964538613191
1103	32-33	0.09456365884497586
1103	34-35	0.11154059129187033
1103	36-37	0.098336412164727
1103	38-39	0.06045441680924313
1103	40-41	0.11000285516005448
1103	42-43	0.07206709846381898
1103	44-45	0.07489744205646076
1103	46-47	0.05378718198848986
1103	48-49	0.06291632479043585
1103	50-51	0.06556081831216432
1103	52-53	0.06443355716309895
1103	54-55	0.030347737200919767
1103	56-57	0.02846479533422297
1103	58-59	0.0700286770096028
1103	60-61	0.06165338020870337
1103	62-63	-0.021997598451079625
1103	64-65	-0.019472881913813467
1103	66-67	-0.00815733296829535
1103	68-69	-0.01648540606726101
1103	70-71	0.01905552249321829
1103	72-73	0.04421657574863502
1103	74-75	0.019254499508150502
1103	76-77	-0.056104301501086695
1103	78-79	-0.04513325221809339
1103	80-81	-0.04882093881361982
1103	82-83	-0.04818833965427416
1103	84-85	-0.030030543700007684
1103	86-87	0.03869707422579438
1103	88-89	7.474952626083109E-4
1103	90-91	-0.0672176829525597
1103	92-93	-0.05573419242508493
1103	94-95	-0.0721672616362028
1103	96-97	-0.03183905777466833
1103	98-99	-0.05940051907278132
1103	100	-0.05223164161515115
1104	1	0.040893604003230166
1104	2	0.09487918153878638
1104	3	0.05782861901827374
1104	4	0.044986648831851994
1104	5	-0.025241399107954976
1104	6	0.0036922061285764585
1104	7	0.0430500222387451
1104	8	0.05451004497221845
1104	9	0.10256229974402231
1104	10-11	0.0703256390003304
1104	12-13	0.12289444506500757
1104	14-15	0.14112656950529612
1104	16-17	0.13368197431916684
1104	18-19	0.18572870878354308
1104	20-21	0.1237524514907733
1104	22-23	0.1061332035441751
1104	24-25	0.13426365286887432
1104	26-27	0.04949169477157511
1104	28-29	0.1043953545291032
1104	30-31	0.10158915809351754
1104	32-33	0.07034018954091437
1104	34-35	0.14278978696521705
1104	36-37	0.11487888151191328
1104	38-39	0.14054175557861726
1104	40-41	0.1498307971730597
1104	42-43	0.15533026523223015
1104	44-45	0.09712005373584987
1104	46-47	0.11692749206514463
1104	48-49	0.12481513302008551
1104	50-51	0.10590332941790592
1104	52-53	0.10554843610567843
1104	54-55	0.07835823620438731
1104	56-57	0.14202440651960302
1104	58-59	0.06483902217653537
1104	60-61	0.0452939224027844
1104	62-63	0.044196189131952224
1104	64-65	0.0761227031730769
1104	66-67	0.035023349628609424
1104	68-69	0.07032039736254347
1104	70-71	0.08920794698263279
1104	72-73	0.12219918895233661
1104	74-75	0.11474026053035402
1104	76-77	0.018423702835541178
1104	78-79	0.02038080881872162
1104	80-81	0.06342214050631867
1104	82-83	0.1352307318651711
1104	84-85	0.105309169170269
1104	86-87	0.11360752740567648
1104	88-89	0.054732257698546505
1104	90-91	0.09494823957001586
1104	92-93	0.13222767318596595
1104	94-95	0.12017572573089552
1104	96-97	0.1492735084207908
1104	98-99	0.14974287921830154
1104	100	0.18743377677940032
1105	1	0.05392526354785332
1105	2	0.03713007205579544
1105	3	0.03151149009100607
1105	4	0.031887645259899955
1105	5	0.003046743720027223
1105	6	0.03104266642349529
1105	7	-0.055136334196248526
1105	8	-0.03949601264088187
1105	9	-0.014387453430366293
1105	10-11	-0.0293456765730582
1105	12-13	-0.004285244723092774
1105	14-15	-0.02272232751868586
1105	16-17	0.03949078240181336
1105	18-19	-0.0029128383687577752
1105	20-21	0.028082491253670128
1105	22-23	0.08662501425788349
1105	24-25	0.01423047392439969
1105	26-27	0.03722412775397288
1105	28-29	0.033110795622320666
1105	30-31	0.04390493552801189
1105	32-33	0.03835106275985822
1105	34-35	0.06338275043955122
1105	36-37	0.002443113657669471
1105	38-39	-0.004451253051627191
1105	40-41	0.007815764492683286
1105	42-43	0.023237838258907573
1105	44-45	0.08410494879721853
1105	46-47	0.08251826308369914
1105	48-49	0.029550582180313256
1105	50-51	0.009005280972594676
1105	52-53	0.01321218367093735
1105	54-55	-0.0012832278179786272
1105	56-57	0.010936841384236118
1105	58-59	-0.030051979332654355
1105	60-61	-0.02649791300829918
1105	62-63	-0.05335076966887442
1105	64-65	-0.05065021825750904
1105	66-67	-0.023100050435978403
1105	68-69	-0.03749202319319522
1105	70-71	-3.917302492197905E-4
1105	72-73	0.021730795181788665
1105	74-75	-0.0651494868919329
1105	76-77	-0.03975262804222268
1105	78-79	-0.0534106472914786
1105	80-81	0.043286976403777544
1105	82-83	0.026094093826166898
1105	84-85	-0.013029757039234369
1105	86-87	-0.012687863649261999
1105	88-89	0.009153625077154004
1105	90-91	-0.017641735948934922
1105	92-93	0.050330499965923536
1105	94-95	-0.03613878026593653
1105	96-97	-0.08303357111940102
1105	98-99	-0.045181676253463365
1105	100	0.02291959324323045
1106	1	0.04440290763256627
1106	2	0.06375550953432452
1106	3	0.05028390107424485
1106	4	0.0163850974629014
1106	5	-0.019595200104575383
1106	6	0.002849023372441195
1106	7	0.012524793817739521
1106	8	0.06292683091140816
1106	9	0.06040845978294129
1106	10-11	0.017036033570576592
1106	12-13	0.027681549139558115
1106	14-15	0.053631836069726546
1106	16-17	0.06657308484825819
1106	18-19	0.04750153886448061
1106	20-21	0.04993433297567407
1106	22-23	0.1014511325880818
1106	24-25	0.09318035044851314
1106	26-27	0.031868650708247515
1106	28-29	0.01826746527973455
1106	30-31	0.06453904702244984
1106	32-33	0.06502046029437025
1106	34-35	0.08903043034410985
1106	36-37	0.08227748327465889
1106	38-39	0.07120478921876838
1106	40-41	0.04023829681405999
1106	42-43	0.11175303523454261
1106	44-45	0.11826971926075913
1106	46-47	0.14569253122473924
1106	48-49	0.1067390699061761
1106	50-51	0.078908502830771
1106	52-53	0.08471122367553363
1106	54-55	0.04970357326691044
1106	56-57	0.040205829141861216
1106	58-59	0.019516258515665186
1106	60-61	0.10223683169957098
1106	62-63	0.04747510302993163
1106	64-65	0.01924386053777738
1106	66-67	0.056652896145273246
1106	68-69	0.07215889623740424
1106	70-71	0.029377139408325093
1106	72-73	0.06664482104895342
1106	74-75	0.026439455880776563
1106	76-77	0.05242812501921179
1106	78-79	0.062076611418113714
1106	80-81	0.08404452396290196
1106	82-83	0.1056842205338242
1106	84-85	0.0919451537674405
1106	86-87	0.1107748704838869
1106	88-89	0.05402780395675677
1106	90-91	0.007604042156629021
1106	92-93	0.07219048923107607
1106	94-95	0.007889364020293499
1106	96-97	0.051601147537290615
1106	98-99	0.0992393682374626
1106	100	0.10805277387431289
1107	1	0.017081009162467353
1107	2	0.06506091701643157
1107	3	0.038068573904645575
1107	4	0.025237067466591157
1107	5	-0.035206827770537075
1107	6	-0.06164281243361813
1107	7	-0.04127223751756759
1107	8	-0.002382163832947981
1107	9	0.004577881230829917
1107	10-11	-0.017895462977449483
1107	12-13	0.0062701896318060335
1107	14-15	0.044538617799169344
1107	16-17	0.06609505795006498
1107	18-19	0.054257947014562546
1107	20-21	0.019481212032118833
1107	22-23	0.04204566732192916
1107	24-25	0.017804416749122254
1107	26-27	0.04941236859939835
1107	28-29	0.003870893716793944
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2216	9	-0.1527927001426832
2216	10-11	-0.1454593529413586
2216	12-13	-0.0959766854488322
2216	14-15	-0.1933954305879766
2216	16-17	-0.17505819585441174
2216	18-19	-0.1341549850924295
2216	20-21	-0.13430270177398285
2216	22-23	-0.1424524101569773
2216	24-25	-0.21378648103667786
2216	26-27	-0.263942545522454
2216	28-29	-0.30451856956103285
2216	30-31	-0.20508755678583412
2216	32-33	-0.22785808284216102
2216	34-35	-0.1954633289421892
2216	36-37	-0.23439323063163897
2216	38-39	-0.2401349677180562
2216	40-41	-0.2851011094611593
2216	42-43	-0.24613171506673837
2216	44-45	-0.1426619452954725
2216	46-47	-0.20753585162729848
2216	48-49	-0.25030786933808713
2216	50-51	-0.174645191274017
2216	52-53	-0.10847824969353326
2216	54-55	-0.12558566779087244
2216	56-57	-0.1470188067749305
2216	58-59	-0.10113056204562554
2216	60-61	-0.09584628581028198
2216	62-63	-0.14077851617997084
2216	64-65	-0.12843992707487928
2216	66-67	-0.12587472413543566
2216	68-69	-0.08989516130760933
2216	70-71	-0.1214471871603422
2216	72-73	-0.14977225141670658
2216	74-75	-0.11769473489942328
2216	76-77	-0.08130881127420508
2216	78-79	-0.1159878622856283
2216	80-81	-0.10529602857496201
2216	82-83	-0.12506050617526654
2216	84-85	-0.09417419223001389
2216	86-87	-0.13312723213080346
2216	88-89	-0.13069378395452702
2216	90-91	-0.16223093837483304
2216	92-93	-0.21191626037195022
2216	94-95	-0.24000571302992313
2216	96-97	-0.19447504485211198
2216	98-99	-0.221321847729115
2216	100	-0.21507536292232743
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25.0
3	7.0
4	26.0
5	53.0
6	93.0
7	258.0
8	494.0
9	873.0
10	1550.0
11	2361.0
12	3584.0
13	5245.0
14	5761.0
15	7719.0
16	9717.0
17	14004.0
18	16131.0
19	8953.0
20	6731.0
21	6944.0
22	7494.0
23	8529.0
24	9986.0
25	12301.0
26	15526.0
27	19459.0
28	25388.0
29	32889.0
30	42297.0
31	53798.0
32	69372.0
33	91258.0
34	129291.0
35	210470.0
36	429124.0
37	958220.0
38	1468435.0
39	522101.0
40	684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80113910599924	18.526163572645434	12.98346665191355	24.689230669441777
2	16.168062862153096	20.09520814272644	38.46246950289722	25.274259492223237
3	18.88759780146104	24.6606805425871	29.5825906668595	26.869130989092362
4	13.166689579110713	16.51592502222348	36.551368230296035	33.76601716836977
5	13.942437867295144	37.23090446608014	34.47597637107631	14.350681295548412
6	32.466720226897145	36.2562256620216	17.357326791742196	13.919727319339056
7	28.568113240275324	31.822284362556445	21.60160516489109	18.007997232277138
8	26.653055798048957	34.547391364015844	19.84972609893985	18.949826738995355
9	26.51687599144752	15.497030601270481	19.799573329114594	38.186520078167405
10-11	25.31895559257551	25.40887405125781	27.905898212495376	21.366272143671303
12-13	26.09914439580563	22.706974326156004	28.068373046383126	23.125508231655235
14-15	23.168994755486345	24.21105950993389	25.369060133214266	27.2508856013655
16-17	23.12802184148247	27.75128891002492	25.772375118264744	23.348314130227863
18-19	23.094197621504787	26.65190982594258	27.75052449287308	22.503368059679552
20-21	23.97634728891098	25.8380267948425	27.402397483435788	22.78322843281073
22-23	23.600823702190155	25.67248045577616	27.320039703150805	23.40665613888288
24-25	23.512466084112354	25.727589305881043	27.467842449205985	23.29210216080062
26-27	23.318709331250624	25.895276864679396	27.40271218512898	23.383301618941
28-29	23.217791991749923	26.002812374495438	27.422593281872814	23.35680235188183
30-31	23.5125462978411	26.0902575518512	27.088956207128316	23.30823994317938
32-33	23.40460138464074	25.936853604645165	27.3611739835488	23.2973710271653
34-35	23.36556078082297	25.833722100378758	27.492473410419432	23.30824370837884
36-37	23.628520585090197	25.92487114731853	27.01584410749961	23.430764160091666
38-39	23.529060599918566	26.07854709571546	27.035526514437926	23.35686578992805
40-41	23.507166553857594	25.981117191149412	27.189099783597243	23.322616471395747
42-43	23.539714503598493	26.011748725036938	26.955876745626995	23.49266002573757
44-45	23.53733270912469	26.0224107425529	26.929685851391238	23.510570696931175
46-47	23.62093524086965	26.118985705392273	26.797072569186913	23.463006484551162
48-49	23.733530915878013	26.08053847677011	26.537632779068073	23.648297828283805
50-51	23.569622623841184	26.236303110406457	26.57616539273412	23.617908873018244
52-53	23.73861225248365	26.122322308250407	26.49485518633196	23.644210252933977
54-55	23.620633970785978	26.22471358352763	26.468114124129155	23.68653832155724
56-57	23.351603677482064	26.24455972894337	26.542578113971064	23.861258479603503
58-59	23.400066521932224	25.981817973882993	26.753336580010256	23.86477892417453
60-61	23.444639551312445	26.22913752969291	26.624288501904875	23.701934417089774
62-63	23.389636548699386	26.09312486538311	26.642635765300582	23.87460282061692
64-65	23.419658848502305	26.089183256409726	26.59979542632739	23.89136246876058
66-67	23.338636557220912	26.050333173899087	26.5405726409638	24.070457627916202
68-69	23.416395636950707	26.05313822449993	26.585182829949318	23.94528330860005
70-71	23.28665113147922	26.079363356725064	26.558380191647235	24.075605320148476
72-73	23.492139810248975	25.954047425009204	26.5233529137698	24.030459850972026
74-75	23.44000189653474	26.13980010380907	26.32403320508295	24.09616479457324
76-77	23.459051511041213	25.999103198631257	26.393824459947595	24.14802083037993
78-79	23.511480827642668	25.97558176090702	26.42613754882927	24.086799862621046
80-81	23.45261265871884	25.906626991870386	26.556750898174776	24.084009451236003
82-83	23.423288839839504	25.85705876908045	26.54979396042129	24.169858430658756
84-85	23.440991589747206	25.859567672169064	26.297568415056066	24.40187232302766
86-87	23.332607840257147	25.9327797904552	26.47557727031384	24.259035098973815
88-89	23.158923755661874	25.83656151518018	26.484894157965726	24.51962057119222
90-91	23.260012539462796	25.72843783620288	26.41404600908307	24.597503615251256
92-93	23.281429064960747	25.7430518327913	26.446445802508844	24.52907329973911
94-95	23.174160281581482	25.682730976321793	26.478592264133454	24.664516477963268
96-97	23.17775796010198	25.498760945222127	26.61630472670628	24.707176367969605
98-99	22.984865209757764	25.72537895348535	26.55058157307183	24.739174263685058
100	23.26497188211718	25.445308019654284	26.287498353049486	25.00222174517905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	72.5
2	143.5
3	260.0
4	184.0
5	133.5
6	303.5
7	670.0
8	1135.5
9	1367.0
10	1075.0
11	755.5
12	789.5
13	931.5
14	1102.0
15	1293.0
16	1464.0
17	1639.0
18	1914.5
19	2160.0
20	2442.0
21	2939.5
22	3562.0
23	4383.0
24	5821.5
25	7816.5
26	10643.5
27	14796.0
28	20808.0
29	28397.5
30	36081.0
31	43684.5
32	52359.5
33	62387.0
34	71294.5
35	79654.5
36	89561.0
37	100918.0
38	111543.0
39	119322.0
40	127816.5
41	133845.0
42	136030.0
43	141738.0
44	146657.0
45	148722.0
46	147789.5
47	145237.0
48	148259.5
49	152492.5
50	152233.0
51	149575.5
52	147745.5
53	148745.0
54	147629.0
55	143225.5
56	133762.5
57	121777.5
58	110992.0
59	98241.0
60	86633.0
61	78133.0
62	69212.5
63	58309.0
64	47260.0
65	39038.5
66	33109.0
67	26962.5
68	22511.5
69	18599.5
70	13948.5
71	9739.0
72	7332.5
73	5874.5
74	4445.0
75	2940.5
76	1767.5
77	1113.0
78	759.0
79	463.0
80	289.5
81	162.5
82	81.5
83	37.5
84	22.0
85	14.0
86	10.0
87	8.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08784530268270072
2	7.385962525532201E-4
3	0.0
4	3.335595979272607E-4
5	0.038478482189466146
6	1.6677979896363035E-4
7	0.004741311427680348
8	3.5738528349349354E-4
9	1.1912842783116453E-4
10-11	3.5738528349349354E-4
12-13	0.0
14-15	7.147705669869871E-5
16-17	0.0
18-19	3.5738528349349354E-5
20-21	0.0
22-23	7.147705669869871E-5
24-25	7.147705669869871E-5
26-27	0.0013223255489259263
28-29	0.008136471620868536
30-31	0.03552409717925326
32-33	0.02488592857393027
34-35	0.02843595572329897
36-37	0.025731740411531534
38-39	0.02457619466156924
40-41	0.03028244635468202
42-43	0.022658226973487492
44-45	0.021550332594657662
46-47	0.015212700234039709
48-49	0.010102090680082752
50-51	0.007243008412134802
52-53	0.004908091226643979
54-55	0.0038001968478141484
56-57	0.002251527286009009
58-59	9.292017370830833E-4
60-61	5.00339396890891E-4
62-63	0.0013104127061428098
64-65	0.0030973391236102776
66-67	4.407751829753087E-4
68-69	9.649402654324325E-4
70-71	2.978210695779113E-4
72-73	4.6460086854154166E-4
74-75	5.360779252402404E-4
76-77	2.8590822679479483E-4
78-79	3.5738528349349354E-5
80-81	6.671191958545214E-4
82-83	4.884265541077746E-4
84-85	2.8590822679479483E-4
86-87	1.3104127061428096E-4
88-89	0.0
90-91	3.5738528349349354E-5
92-93	2.3825685566232903E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4197151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.622553130579288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.58601214214457	17.058447042588618
2	15.087404981314952	8.938549113231376
3	7.346150297957833	6.528351825194304
4	4.3610130953978405	5.167373684862984
5	2.9548721724963403	4.376542896192155
6	2.201197567537237	3.9123055137164213
7	1.633916351101148	3.38805417449912
8	1.2403928669180684	2.939488288245764
9	1.0151901369179483	2.706527139164279
>10	6.160532077043968	32.64306304982515
>50	0.3050639910882216	6.094295915862942
>100	0.10447015856969001	5.193556396567714
>500	0.002898506738947446	0.5497976840002408
>1k	8.051407028912187E-4	0.38286923911598814
>5k	8.051407028912105E-5	0.12077803693286934
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5064	0.12065327170740342	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4293	0.10228366813583786	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.7651371132465805E-5	0.0	0.0	0.0
6	0.0	4.7651371132465805E-5	0.0	0.0	0.0
7	0.0	4.7651371132465805E-5	0.0	0.0	0.0
8	0.0	4.7651371132465805E-5	0.0	0.0	0.0
9	0.0	4.7651371132465805E-5	0.0	0.0	0.0
10-11	0.0	5.956421391558226E-5	0.0	0.0	0.0
12-13	0.0	7.147705669869871E-5	0.0	2.3825685566232903E-5	0.0
14-15	0.0	7.147705669869871E-5	0.0	2.3825685566232903E-5	0.0
16-17	0.0	7.147705669869871E-5	0.0	1.1912842783116453E-4	0.0
18-19	0.0	8.338989948181516E-5	0.0	1.1912842783116453E-4	0.0
20-21	0.0	9.530274226493161E-5	0.0	1.3104127061428096E-4	0.0
22-23	0.0	9.530274226493161E-5	0.0	2.2634401287921258E-4	0.0
24-25	0.0	1.1912842783116453E-4	0.0	4.526880257584252E-4	0.0
26-27	0.0	1.1912842783116453E-4	0.0	8.815503659506175E-4	0.0
28-29	0.0	1.1912842783116453E-4	0.0	0.002549348355586921	0.0
30-31	0.0	1.1912842783116453E-4	0.0	0.009518361383710046	0.0
32-33	0.0	1.3104127061428096E-4	0.0	0.025338616599688692	0.0
34-35	0.0	1.4295411339739742E-4	0.0	0.04755606839020088	0.0
36-37	0.0	1.5486695618051387E-4	0.0	0.08665401840438908	0.0
38-39	0.0	1.6677979896363032E-4	0.0	0.1474929064977648	0.0
40-41	0.0	1.6677979896363032E-4	0.0	0.21754042206248952	0.0
42-43	0.0	1.6677979896363032E-4	0.0	0.2907448409647401	0.0
44-45	0.0	1.6677979896363032E-4	0.0	0.37570723569392667	0.0
46-47	0.0	1.9060548452986322E-4	0.0	0.47725230757721127	0.0
48-49	0.0	2.1443117009609612E-4	0.0	0.5857187411174866	0.0
50-51	0.0	2.1443117009609612E-4	0.0	0.699212394312237	0.0
52-53	0.0	2.1443117009609612E-4	0.0	0.8157914737878147	0.0
54-55	0.0	2.1443117009609612E-4	0.0	0.9458678041366633	0.0
56-57	0.0	2.501696984454455E-4	0.0	1.0960768387889783	0.0
58-59	0.0	2.8590822679479483E-4	0.0	1.264190876144318	0.0
60-61	0.0	2.8590822679479483E-4	0.0	1.4378682110793726	0.0
62-63	0.0	3.216467551441442E-4	0.0	1.6161796418570598	0.0
64-65	0.0	3.5738528349349354E-4	0.0	1.8100611581522799	0.0
66-67	0.0	3.8121096905972644E-4	0.0	2.0268034197483007	0.0
68-69	0.0	4.0503665462595935E-4	0.0	2.2681695273770233	0.0
70-71	0.0	4.0503665462595935E-4	0.0	2.525808578247483	0.0
72-73	0.0	4.0503665462595935E-4	0.0	2.7912386282981005	0.0
74-75	0.0	5.479907680233568E-4	0.0	3.0689627321008945	0.0
76-77	0.0	6.432935102882884E-4	0.0	3.376135383263552	0.0
78-79	0.0	6.432935102882884E-4	0.0	3.7223940715976145	0.0
80-81	0.0	6.552063530714048E-4	0.0	4.081828364049804	0.0
82-83	0.0	6.671191958545213E-4	0.0	4.45959652154521	0.0
84-85	0.0	6.671191958545213E-4	0.0	4.845667930460448	0.0
86-87	0.0	6.671191958545213E-4	0.0	5.26413035890298	0.0
88	0.0	6.671191958545213E-4	0.0	5.599298190605961	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11060	0.0	37.26902	1
GGTATCA	7430	0.0	34.198254	1
TCAACGC	12685	0.0	31.765665	4
CAACGCA	12900	0.0	31.23661	5
ATCAACG	13085	0.0	30.866476	3
AACGCAG	13275	0.0	30.306156	6
TATCAAC	13530	0.0	30.094896	2
GTACATG	14030	0.0	27.736193	1
TACATGG	14340	0.0	26.230925	2
ACGCAGA	15270	0.0	25.977364	7
CGCAGAG	15815	0.0	25.05005	8
ACATGGG	14510	0.0	24.853956	3
GCAGAGT	17440	0.0	22.311775	9
GAGTACT	8195	0.0	21.275023	12-13
TATACTG	1645	0.0	21.151375	5
CATGGGG	11565	0.0	20.815742	4
AGAGTAC	14660	0.0	19.185822	10-11
GAGTACA	9550	0.0	18.723164	1
GTACTTT	9215	0.0	18.639622	14-15
AGTACTT	8910	0.0	18.592016	12-13
>>END_MODULE