FastQCFastQC Report
Wed 25 May 2016
SRR1033194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894217
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97481.0901157101687846No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19810.2215345939520273No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13400.14985176976058384No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11400.12748583397542207No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9560.10690917305307326No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9530.10657368401629581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA8900.040.2321741
GTACAAG8650.039.7609441
GAGAACA16900.035.6839751
CGCAGAA1850.035.5839462
TACATGA4800.035.2662352
TACAAGA7050.034.015662
ACGCAAA4000.031.8019451
GTACATG85400.030.563411
TCTCGCT4800.030.3681472
GTACATA4100.029.8771671
TCTGTCG5050.029.7592268
CTGTCGC5450.029.2985089
TACATGG84450.028.8421692
CGCAGGA1503.6794518E-828.2129862
CTCGCTC5100.027.638123
CGCAAAG1555.0582457E-827.3028932
CCGACAC350.00861702726.99651192-93
CGCTCTG4750.026.7041655
GTATCAA45950.026.5560911
GTCTCGC5850.024.9663931
Produced by FastQC (version 0.11.5)