FastQCFastQC Report
Wed 25 May 2016
SRR1033194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894217
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150331.6811355632916845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122991.375393221108523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69310.775091504634781No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.30428855635712587No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.2824817689665931No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.24759090914174076No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.22802071532972423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG17310.1935771742205751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16600.18563726701684266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15970.17859199724451671No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14630.16360682026845833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG14190.15868631439572276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12400.13866880186800296No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11460.12815681204897691No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10460.11697384415639603No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.10948125566836685No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.10344245300637318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67750.051.046081
TATAACG652.7777824E-643.3992272
GTATCAA120850.041.426611
ACGTGCG1550.039.412799
TATCAAC148400.033.519372
ATCAACG152600.032.5352023
AACACGT1900.032.1579326
TCAACGC154250.032.1566964
CAACGCA156200.031.7552535
AACGCAG158600.031.2938586
TCTGTCG6050.031.0692068
CTGTCGC6200.030.317539
GTCCTAA4250.029.8922941
ACGCAGA175450.028.1544937
CGCAGAG175650.027.95728
TACAGCG856.814848E-427.647097
CCCGTAC1406.3036714E-726.8872491
GCGAGCT1755.5606506E-926.8526699
GCAGAGT185500.026.4220149
GTCTAAC1807.405106E-926.1403791
Produced by FastQC (version 0.11.5)