##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033194_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 894217 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65296119398312 34.0 31.0 34.0 31.0 34.0 2 32.69169228498228 34.0 31.0 34.0 31.0 34.0 3 32.758957836856155 34.0 31.0 34.0 31.0 34.0 4 36.25923014212434 37.0 35.0 37.0 35.0 37.0 5 36.2388089244557 37.0 37.0 37.0 35.0 37.0 6 36.3660576795118 37.0 37.0 37.0 35.0 37.0 7 36.32877590115151 37.0 37.0 37.0 35.0 37.0 8 36.34419833217217 37.0 37.0 37.0 35.0 37.0 9 38.12937128236211 39.0 39.0 39.0 37.0 39.0 10-11 38.116522052253536 39.0 39.0 39.0 37.0 39.0 12-13 38.067730204189814 39.0 39.0 39.0 36.0 39.0 14-15 39.41710233645748 41.0 39.0 41.0 36.0 41.0 16-17 39.476468798960425 41.0 39.0 41.0 36.5 41.0 18-19 39.622769417266724 41.0 40.0 41.0 37.0 41.0 20-21 39.64503806123122 41.0 40.0 41.0 37.0 41.0 22-23 39.51947234284296 41.0 40.0 41.0 36.0 41.0 24-25 39.42251489291749 41.0 39.0 41.0 36.0 41.0 26-27 39.22223856178086 41.0 39.0 41.0 35.0 41.0 28-29 39.055115257258585 41.0 39.0 41.0 35.0 41.0 30-31 38.67700066091341 40.0 38.0 41.0 35.0 41.0 32-33 38.30788667627657 40.0 36.5 41.0 35.0 41.0 34-35 38.04266022676822 40.0 36.0 41.0 35.0 41.0 36-37 37.78814314646221 40.0 36.0 41.0 34.5 41.0 38-39 37.544316424313116 40.0 35.5 41.0 34.0 41.0 40-41 37.22492806555903 40.0 35.0 41.0 33.0 41.0 42-43 36.82405445210726 40.0 35.0 41.0 33.0 41.0 44-45 36.23147457496334 40.0 35.0 41.0 30.5 41.0 46-47 35.28530770495305 40.0 35.0 41.0 18.5 41.0 48-49 34.45221014585945 40.0 35.0 41.0 2.0 41.0 50-51 34.051309134136346 40.0 35.0 41.0 2.0 41.0 52-53 33.82951900936797 40.0 35.0 41.0 2.0 41.0 54-55 33.64329072249801 40.0 35.0 41.0 2.0 41.0 56-57 33.461111229153545 39.5 35.0 41.0 2.0 41.0 58-59 33.25641091591862 39.0 35.0 41.0 2.0 41.0 60-61 33.04980782069677 39.0 35.0 41.0 2.0 41.0 62-63 32.79395884891475 38.5 34.5 41.0 2.0 41.0 64-65 32.539042536654975 38.0 34.0 41.0 2.0 41.0 66-67 32.26560107893274 37.0 34.0 40.5 2.0 41.0 68-69 31.946751739231082 37.0 34.0 40.0 2.0 41.0 70-71 31.59866564827106 36.0 33.5 39.5 2.0 41.0 72-73 31.2572457244718 36.0 33.0 39.0 2.0 41.0 74-75 30.961018969668437 35.0 33.0 39.0 2.0 41.0 76-77 30.649365310657256 35.0 33.0 37.5 2.0 40.0 78-79 30.32953075148426 35.0 33.0 37.0 2.0 39.0 80-81 30.029927858674125 35.0 32.5 37.0 2.0 39.0 82-83 29.744424451782955 35.0 32.0 36.0 2.0 39.0 84-85 29.495540232404437 35.0 32.0 36.0 2.0 37.0 86-87 29.28034246720874 35.0 32.0 35.5 2.0 37.0 88-89 29.077183726097804 35.0 32.0 35.0 2.0 36.5 90-91 28.878999169105484 35.0 31.5 35.0 2.0 36.0 92-93 28.70987467247883 35.0 31.0 35.0 2.0 36.0 94-95 28.56242724081515 35.0 31.0 35.0 2.0 36.0 96-97 28.443547818929858 35.0 31.0 35.0 2.0 35.5 98-99 28.338319445951036 35.0 31.0 35.0 2.0 35.0 100 28.235030199604793 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.051162817744057065 1101 2 0.1353833271567666 1101 3 0.10919985501037388 1101 4 0.11107407280612591 1101 5 0.010196713619357922 1101 6 0.05726723929222288 1101 7 0.11157805044078373 1101 8 0.12370665598306374 1101 9 0.18954537687267958 1101 10-11 0.25468758222248056 1101 12-13 0.18713609984742163 1101 14-15 0.19598449473628676 1101 16-17 0.2380919448039478 1101 18-19 0.18627921386477198 1101 20-21 0.15307098591036095 1101 22-23 0.21607474429661977 1101 24-25 0.2748713581915041 1101 26-27 0.2383800763853685 1101 28-29 0.2897173622286715 1101 30-31 0.2398488280954183 1101 32-33 0.19827269542657433 1101 34-35 0.31845667141549683 1101 36-37 0.5132623802033507 1101 38-39 0.4945992929708325 1101 40-41 0.6093600508716861 1101 42-43 0.5612423596048473 1101 44-45 0.3721878798764564 1101 46-47 0.6177102599900195 1101 48-49 0.7321908529154513 1101 50-51 0.5728251384911829 1101 52-53 0.43502072211865084 1101 54-55 0.34992957406132774 1101 56-57 0.31471578041808357 1101 58-59 0.4367743499272905 1101 60-61 0.5111056214622565 1101 62-63 0.5406825966069704 1101 64-65 0.5850534653426394 1101 66-67 0.6578856242370037 1101 68-69 0.6065944590036807 1101 70-71 0.5443034350873006 1101 72-73 0.5193489033552119 1101 74-75 0.42730444330445394 1101 76-77 0.5564450637672209 1101 78-79 0.45852726735511595 1101 80-81 0.38327192553024503 1101 82-83 0.3883022161471459 1101 84-85 0.5465271419645887 1101 86-87 0.3598534229085395 1101 88-89 0.3631929819178872 1101 90-91 0.4750932457946213 1101 92-93 0.449816289408588 1101 94-95 0.44640219779976675 1101 96-97 0.3665695972609626 1101 98-99 0.39314110227755705 1101 100 0.3376511386069083 1102 1 0.05148036011380697 1102 2 0.10776637187116478 1102 3 0.11110141687574782 1102 4 -0.023851895513487875 1102 5 -0.021880604255322567 1102 6 2.8869186394331336E-4 1102 7 0.015043323859529778 1102 8 0.07321926536902623 1102 9 0.04955903857928945 1102 10-11 0.13901354315738246 1102 12-13 0.03891396788272061 1102 14-15 0.10413905663858714 1102 16-17 0.022525907068100537 1102 18-19 0.11236753495180096 1102 20-21 0.11677423148876898 1102 22-23 0.09185660007330654 1102 24-25 0.07773847040211734 1102 26-27 0.2046790462188426 1102 28-29 0.2097188391699234 1102 30-31 0.06993781380602115 1102 32-33 0.03184357014502126 1102 34-35 0.08115282767587928 1102 36-37 0.10575305701167537 1102 38-39 0.12720000190262937 1102 40-41 0.06379364390052444 1102 42-43 0.04327075380047063 1102 44-45 0.01514287009171511 1102 46-47 -0.38160203923828107 1102 48-49 -0.37659417828493247 1102 50-51 -0.4584555541942663 1102 52-53 -0.32068765583061776 1102 54-55 -0.4750288869658874 1102 56-57 -0.46176669936627945 1102 58-59 -0.6121456367802409 1102 60-61 -0.6084823119276876 1102 62-63 -0.5681125885645457 1102 64-65 -0.6809787319767153 1102 66-67 -0.8359296842590993 1102 68-69 -0.8186501593916198 1102 70-71 -0.8090279905302467 1102 72-73 -0.7083382066399899 1102 74-75 -0.7047285663325873 1102 76-77 -0.7844735936931784 1102 78-79 -0.7540304256626449 1102 80-81 -0.6988700085243273 1102 82-83 -0.7730794623965913 1102 84-85 -0.6684591963288042 1102 86-87 -0.6112954527699337 1102 88-89 -0.6878205690181254 1102 90-91 -0.6418241931892439 1102 92-93 -0.7014391106578728 1102 94-95 -0.6683173679795047 1102 96-97 -0.6201185465931154 1102 98-99 -0.5194793607435244 1102 100 -0.49297693066163717 1103 1 0.0831414019589829 1103 2 0.18134165996111307 1103 3 0.1425858451549047 1103 4 0.026243405866196667 1103 5 0.05097249172803231 1103 6 0.036325728253331135 1103 7 0.136610288379444 1103 8 0.06089391170473135 1103 9 0.08938015176934755 1103 10-11 0.10160906311821094 1103 12-13 0.11028420937117289 1103 14-15 0.10582300578786175 1103 16-17 0.1794728530523244 1103 18-19 0.1063259520745774 1103 20-21 0.0680739149832732 1103 22-23 0.05758832231787636 1103 24-25 0.14054207961400067 1103 26-27 0.21304676965249314 1103 28-29 0.21492806504294037 1103 30-31 0.20239886264579354 1103 32-33 0.21229279811641533 1103 34-35 0.49703580807352665 1103 36-37 0.5543283451016308 1103 38-39 0.6339274924162055 1103 40-41 0.5306292009327223 1103 42-43 0.45250755587086644 1103 44-45 0.5436878191185954 1103 46-47 0.6701601619612916 1103 48-49 0.817252762453343 1103 50-51 0.7644504384264366 1103 52-53 0.7403253366327149 1103 54-55 0.7253383293595022 1103 56-57 0.8153115701705005 1103 58-59 0.82135935561314 1103 60-61 0.8001280365780943 1103 62-63 0.7682225925080814 1103 64-65 0.6795936008961476 1103 66-67 0.6074534272872256 1103 68-69 0.680608413360936 1103 70-71 0.7277631508130682 1103 72-73 0.7811445429359694 1103 74-75 0.6928906478244734 1103 76-77 0.6884555685290081 1103 78-79 0.729179045021251 1103 80-81 0.714035896663912 1103 82-83 0.6477119762170815 1103 84-85 0.7607734089835922 1103 86-87 0.6493560064777988 1103 88-89 0.5558606872114389 1103 90-91 0.5145754256052371 1103 92-93 0.5895326136850727 1103 94-95 0.5326819850203037 1103 96-97 0.5759448069662589 1103 98-99 0.5478523754902334 1103 100 0.5659193219831948 1104 1 0.10310805078614749 1104 2 0.15930769528333855 1104 3 0.17702288910535202 1104 4 0.0476369431790431 1104 5 0.10262112308988236 1104 6 0.08174630547011219 1104 7 0.06934175596158099 1104 8 0.14522962944024442 1104 9 0.15155545101617207 1104 10-11 0.10253666300856423 1104 12-13 0.21588048396031922 1104 14-15 0.27074814513181167 1104 16-17 0.21432433917014038 1104 18-19 0.20208983099220035 1104 20-21 0.24512760645555431 1104 22-23 0.14608029127713706 1104 24-25 0.23580218286557653 1104 26-27 0.16252684053189626 1104 28-29 0.22818980648323617 1104 30-31 0.18455215077120357 1104 32-33 0.23546933416309912 1104 34-35 0.29677865813030024 1104 36-37 0.31618522549427297 1104 38-39 0.18180902395950227 1104 40-41 0.08523929896297489 1104 42-43 0.015335927863716847 1104 44-45 -0.21399445385194582 1104 46-47 -0.0027238170751147095 1104 48-49 0.13849683952000902 1104 50-51 0.2651908050935319 1104 52-53 0.2755553683522223 1104 54-55 0.3342007835098002 1104 56-57 0.36382292327522947 1104 58-59 0.30335396460164077 1104 60-61 0.3053048924305344 1104 62-63 0.33296368036981505 1104 64-65 0.3297872791845933 1104 66-67 0.27378995432712827 1104 68-69 0.3162970237069729 1104 70-71 0.41657990210860163 1104 72-73 0.4964219581221698 1104 74-75 0.5132507719613244 1104 76-77 0.3952467092565968 1104 78-79 0.35823372528075126 1104 80-81 0.3289485537929693 1104 82-83 0.2675330807870395 1104 84-85 0.22552665272780104 1104 86-87 0.18269371810437107 1104 88-89 0.15128885165686867 1104 90-91 0.14208492187852073 1104 92-93 0.229752757532399 1104 94-95 0.2691942583824414 1104 96-97 0.20092849034550042 1104 98-99 0.2618619892431866 1104 100 0.22990707146432854 1105 1 -0.060197268833015016 1105 2 0.008714569096191838 1105 3 -0.03364068948237531 1105 4 0.013480669660069111 1105 5 0.03706365615084195 1105 6 0.06935665800089907 1105 7 -0.0848486536717914 1105 8 0.08171943340397547 1105 9 -0.01342606232169885 1105 10-11 -0.08241731446100431 1105 12-13 0.07996170581220952 1105 14-15 0.06392749263327602 1105 16-17 0.09835487949436583 1105 18-19 0.04923440326041373 1105 20-21 0.03259632248369826 1105 22-23 0.0684165245375965 1105 24-25 -0.022009477170975345 1105 26-27 -0.020247338469175702 1105 28-29 0.041082162741226114 1105 30-31 0.19384438637311519 1105 32-33 0.23821209479815764 1105 34-35 0.2470289299051487 1105 36-37 0.03437300996188952 1105 38-39 0.0837785702448457 1105 40-41 0.2207130831438917 1105 42-43 0.24953005573527065 1105 44-45 0.5463557623733166 1105 46-47 0.5105375257912428 1105 48-49 0.30309159635618954 1105 50-51 0.17496939819582735 1105 52-53 0.252730015044925 1105 54-55 0.2445751906822693 1105 56-57 0.16622432276632537 1105 58-59 0.1608767518766001 1105 60-61 0.25384425199760585 1105 62-63 0.18153133737204996 1105 64-65 0.08757392697635424 1105 66-67 0.19200812680560375 1105 68-69 0.1522573893785264 1105 70-71 0.13660030015421398 1105 72-73 0.09343736267842928 1105 74-75 -0.03965983203585566 1105 76-77 0.023908949875881547 1105 78-79 0.09296654970823326 1105 80-81 0.15752172095540118 1105 82-83 0.08661615429167924 1105 84-85 0.19448869683297687 1105 86-87 0.15574209103918335 1105 88-89 0.20220295587505532 1105 90-91 0.25386651047711695 1105 92-93 0.22633466628773036 1105 94-95 0.17646714719965217 1105 96-97 0.08204790402861661 1105 98-99 0.22349917879269654 1105 100 0.20611408026863742 1106 1 0.17301872891241032 1106 2 0.12872001585211734 1106 3 0.13180790912964824 1106 4 0.13829470765728757 1106 5 0.07046974838799258 1106 6 -0.008424913537226075 1106 7 0.08931543188292324 1106 8 0.13802425662829165 1106 9 0.09368466399372011 1106 10-11 0.06620324717812309 1106 12-13 0.05314930455159583 1106 14-15 0.1097662794971015 1106 16-17 0.21053603426081935 1106 18-19 0.1872740995427975 1106 20-21 0.1486748885506799 1106 22-23 0.2491505900666766 1106 24-25 0.18602408134912451 1106 26-27 0.20056035310895481 1106 28-29 0.16306442962142853 1106 30-31 0.2238259324388423 1106 32-33 0.2073860983263458 1106 34-35 0.15865599242359707 1106 36-37 0.13070031121626613 1106 38-39 0.20075476710849216 1106 40-41 0.3401047536720654 1106 42-43 0.269530624799323 1106 44-45 0.2914208253632822 1106 46-47 0.1979536904621213 1106 48-49 0.050612102985788 1106 50-51 0.03263191340474236 1106 52-53 -0.013455453769005032 1106 54-55 0.004569039370849737 1106 56-57 -0.03723538343403732 1106 58-59 0.15029182177663358 1106 60-61 0.16016106621444948 1106 62-63 0.10753844525304856 1106 64-65 0.1913749361166026 1106 66-67 0.10424151352255961 1106 68-69 0.24852236457004295 1106 70-71 0.16641959521934524 1106 72-73 0.20122385255586295 1106 74-75 0.211152439152972 1106 76-77 0.1939940655991208 1106 78-79 0.11901647288458506 1106 80-81 0.1162002187346971 1106 82-83 0.1728395200616646 1106 84-85 0.1503469402677382 1106 86-87 0.14068628211463263 1106 88-89 0.12042133826983914 1106 90-91 0.1580537379699578 1106 92-93 0.14115085859886278 1106 94-95 0.18501767331291674 1106 96-97 0.12687795711415717 1106 98-99 0.23789751549977112 1106 100 0.3136238288778195 1107 1 -0.02985299252668483 1107 2 -0.056218771267310785 1107 3 -0.09671363981490799 1107 4 0.010475274008229007 1107 5 -0.05270569584367735 1107 6 -0.09565173588624276 1107 7 -0.12412970210377239 1107 8 -0.052752005573211136 1107 9 -0.08212184298574954 1107 10-11 -0.10622386253919558 1107 12-13 -0.030821330919749812 1107 14-15 -0.044848041350093126 1107 16-17 -0.10553813961661973 1107 18-19 -0.12799215748510306 1107 20-21 -0.10191449843781442 1107 22-23 -0.06129360482395185 1107 24-25 -0.11903747480415205 1107 26-27 -0.12090596080413718 1107 28-29 -0.17837974320167405 1107 30-31 -0.2196211140672517 1107 32-33 -0.2070688091248698 1107 34-35 -0.28931734999827796 1107 36-37 -0.11032341383209854 1107 38-39 -0.08779261310772313 1107 40-41 0.02674887059075104 1107 42-43 0.06218103341645076 1107 44-45 -0.01662996183460308 1107 46-47 -0.07009846982661827 1107 48-49 -0.13132672581675564 1107 50-51 -0.09289096449560219 1107 52-53 -0.09952097227017731 1107 54-55 -0.1622412705798979 1107 56-57 -0.1540924642919208 1107 58-59 -0.1722616245588 1107 60-61 -0.09412647126969631 1107 62-63 -0.06845287730315874 1107 64-65 -0.2258555764140766 1107 66-67 -0.1579912548002369 1107 68-69 -0.1721421010646793 1107 70-71 -0.09567310557680031 1107 72-73 -0.1281625597293754 1107 74-75 -0.19040675454846578 1107 76-77 -0.25530578066373977 1107 78-79 -0.24978622867166678 1107 80-81 -0.32442279671992935 1107 82-83 -0.3183291764092502 1107 84-85 -0.18657522754848443 1107 86-87 -0.21984937043453812 1107 88-89 -0.12418223078559976 1107 90-91 -0.14794323632627382 1107 92-93 -0.16553358060386358 1107 94-95 -0.23912855841087577 1107 96-97 -0.20549952517664494 1107 98-99 -0.19138309523142638 1107 100 -0.138878261407811 1108 1 0.09749176211126098 1108 2 0.0017326981587899581 1108 3 -0.05461274877538358 1108 4 -0.0036056459862336965 1108 5 -0.025841187522118503 1108 6 0.05040775449088386 1108 7 0.029031781788404487 1108 8 0.08631545396422524 1108 9 0.12311522913552153 1108 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-0.2836166258966344 2214 72-73 -0.31884171695153185 2214 74-75 -0.19300741670555865 2214 76-77 -0.1758513909726851 2214 78-79 -0.40630115844017567 2214 80-81 -0.2820918163347699 2214 82-83 -0.18016590381827058 2214 84-85 -0.22083962634196652 2214 86-87 -0.07982880072956178 2214 88-89 -0.1227755931515162 2214 90-91 0.01918940926797319 2214 92-93 -0.027920023338875666 2214 94-95 -0.06366072032702874 2214 96-97 -0.24116944611044744 2214 98-99 -0.34784158101557594 2214 100 -0.42653331504075 2215 1 -0.12522779356140745 2215 2 -0.11587663598792375 2215 3 -0.13952802361027494 2215 4 -0.11945345713749589 2215 5 -0.09147837394635872 2215 6 -0.1316680270946975 2215 7 -0.11532526833438794 2215 8 -0.12856508373344866 2215 9 -0.19079803510857118 2215 10-11 -0.14738391665526507 2215 12-13 -0.10437369916110129 2215 14-15 -0.24472693114976352 2215 16-17 -0.22152447875088654 2215 18-19 -0.186899561875002 2215 20-21 -0.17169528524364353 2215 22-23 -0.16901513677469637 2215 24-25 -0.2245820498674007 2215 26-27 -0.15755019243849233 2215 28-29 -0.21586855367229418 2215 30-31 -0.2229181640947573 2215 32-33 -0.2494714762857697 2215 34-35 -0.31015630660136395 2215 36-37 -0.22317088007485353 2215 38-39 -0.30358167653737667 2215 40-41 -0.35189879160093795 2215 42-43 -0.2790412550066037 2215 44-45 -0.34779608182616784 2215 46-47 -0.592328265755306 2215 48-49 -0.7420088445030899 2215 50-51 -0.639031836265147 2215 52-53 -0.626143656156799 2215 54-55 -0.7735100751048805 2215 56-57 -0.8290289135697222 2215 58-59 -0.850857500841478 2215 60-61 -0.8220812844361731 2215 62-63 -0.8769818382307051 2215 64-65 -0.7033842649369717 2215 66-67 -0.84469852924855 2215 68-69 -0.8095739843511005 2215 70-71 -0.833443400864283 2215 72-73 -0.7065854569789174 2215 74-75 -0.7028341288536133 2215 76-77 -0.7384291302211139 2215 78-79 -0.7461693001109282 2215 80-81 -0.7532602922057663 2215 82-83 -0.7689745930246055 2215 84-85 -0.7696617589415666 2215 86-87 -0.7999657788557819 2215 88-89 -0.7503252834509162 2215 90-91 -0.7953442180610644 2215 92-93 -0.7687948490144976 2215 94-95 -0.8354891160402182 2215 96-97 -0.8538068639738263 2215 98-99 -0.8814203819151523 2215 100 -0.8399500099762349 2216 1 -0.024331900122625427 2216 2 -0.10034252971812663 2216 3 -0.07091337996133262 2216 4 -0.0835031129252144 2216 5 -0.026352928689675537 2216 6 -0.07859645649587321 2216 7 -0.025925908114160734 2216 8 -0.009838747627135547 2216 9 -0.15129432398236276 2216 10-11 -0.21048931443284857 2216 12-13 -0.1324152030246637 2216 14-15 -0.17052135986497774 2216 16-17 -0.17477112573546805 2216 18-19 -0.09447013043612884 2216 20-21 -0.13941851884396783 2216 22-23 -0.24706045151989287 2216 24-25 -0.1497374553788262 2216 26-27 -0.15805093107701396 2216 28-29 -0.22497445919381676 2216 30-31 -0.23798123418583828 2216 32-33 -0.22997139110135123 2216 34-35 -0.21917599092585505 2216 36-37 -0.34768779478508094 2216 38-39 -0.24551294728706807 2216 40-41 -0.22896647510049206 2216 42-43 -0.2820805732174634 2216 44-45 -0.11758534567591283 2216 46-47 -0.09975752346282007 2216 48-49 -0.18876340242961476 2216 50-51 -0.10878828730317025 2216 52-53 -0.1213384426465538 2216 54-55 -0.0670706939023944 2216 56-57 -0.01986483606086864 2216 58-59 0.021375266688018257 2216 60-61 0.04590487197585702 2216 62-63 -0.016201330441944606 2216 64-65 0.022243886045679062 2216 66-67 -0.014401852113167024 2216 68-69 0.062262703678918285 2216 70-71 -0.014816567998344965 2216 72-73 -0.030833900682107895 2216 74-75 -0.024584903919688372 2216 76-77 -0.005449480329048129 2216 78-79 0.0405470032890527 2216 80-81 -0.02120814365311574 2216 82-83 -0.06394593855724295 2216 84-85 -0.07672957133858915 2216 86-87 -0.15770682832775051 2216 88-89 -0.14343201503707093 2216 90-91 -0.15580094101669317 2216 92-93 -0.16223923592210454 2216 94-95 -0.1931490441714736 2216 96-97 -0.26080299817066077 2216 98-99 -0.14615770568989817 2216 100 -0.11375709193933048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 7.0 4 20.0 5 65.0 6 142.0 7 310.0 8 476.0 9 825.0 10 1397.0 11 2480.0 12 4571.0 13 7511.0 14 7910.0 15 10331.0 16 14325.0 17 24142.0 18 29631.0 19 12687.0 20 5453.0 21 3706.0 22 3230.0 23 3278.0 24 3441.0 25 3950.0 26 4484.0 27 5196.0 28 5600.0 29 6675.0 30 8447.0 31 10476.0 32 13081.0 33 16967.0 34 24779.0 35 43125.0 36 67024.0 37 148881.0 38 260969.0 39 137900.0 40 719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.32124549504175 18.604414299465002 13.639894343339376 19.434445862153872 2 16.324538193660075 21.482410093423663 41.08505199025728 21.107999722658985 3 18.69503565134151 29.348725587553794 30.50169086663625 21.45454789446845 4 11.09856130796285 17.759599201534307 39.65355087982197 31.488288610680875 5 10.378965952024274 38.76745723725061 34.29377273230726 16.55980407841786 6 27.64544584306253 39.272614222961785 17.07752936650653 16.004410567469158 7 22.962351593883923 37.101752690769516 21.992623310160955 17.943272405185603 8 26.160992962526823 32.291523384249615 20.63333903667247 20.914144616551088 9 27.768632564464436 13.452708188420221 21.81804355556947 36.960615691545875 10-11 23.74676322068139 26.961987308414255 27.22456412862909 22.06668534227526 12-13 27.15465038128329 26.157297389783462 28.494258105135557 18.19379412379769 14-15 23.4839661312445 24.76313062888014 30.71196964046179 21.040933599413563 16-17 17.91002631352345 28.671452231393495 30.313559236740073 23.104962218342976 18-19 17.803676288865006 23.847287627052495 38.77313895844073 19.57589712564176 20-21 20.656171824065076 22.55503977222531 38.87563085917624 17.913157544533373 22-23 19.48632011670578 22.68197395591326 39.47621068546752 18.355495241913435 24-25 18.81580131880814 23.47680902779137 39.349027892014966 18.358361761385524 26-27 18.785345642218505 22.967384436454726 39.56788432182777 18.679385599498996 28-29 18.383324945478947 22.88631661354359 39.71514846502265 19.015209975954818 30-31 19.058368827449655 23.86619720279329 38.91344656026355 18.1619874094935 32-33 18.322563469860974 23.21324105963376 39.76065064367505 18.703544826830214 34-35 18.681863840658984 22.627580868518603 39.660026711947225 19.030528578875188 36-37 19.2810698773726 24.002554784124097 38.085410773269615 18.630964565233686 38-39 19.42234891382827 23.80294049055154 37.89339225723392 18.881318338386272 40-41 18.898923297458165 23.424306709493898 38.11267484321508 19.564095149832855 42-43 19.13156350893441 23.471564862336116 37.48089438601142 19.915977242718057 44-45 20.007012999214812 23.53677742060323 36.33745836381247 20.118751216369482 46-47 20.245394999158382 24.35009358473431 35.15805390726751 20.246457508839804 48-49 20.990931494393767 24.873271870073822 33.043650396443056 21.092146239089356 50-51 21.655524893545042 25.020144661315257 31.79244148821656 21.531888956923137 52-53 21.80528823840577 25.06478052904751 31.35752253758501 21.772408694961715 54-55 21.499509889167292 25.59094281891174 31.249779827563746 21.659767464357227 56-57 21.101366811377346 24.543445635083057 31.654566438305608 22.700621115233986 58-59 20.10910194982272 23.821598853512516 32.82754209164631 23.241757105018454 60-61 20.554710927535762 24.514279867168188 31.987327411183124 22.943681794112926 62-63 20.204181139468982 24.33113846250193 32.15131636334357 23.31336403468552 64-65 19.747984913762977 24.016025632064682 32.79959292881566 23.436396525356678 66-67 19.97054398021951 24.17764417821451 31.86103557927097 23.990776262295007 68-69 21.364632416380243 24.357828097715135 30.958503664960695 23.319035820943927 70-71 21.317424398732072 24.315530557824772 30.661658919644 23.705386123799155 72-73 21.188775841129754 24.55625834531779 30.759527361459337 23.495438452093122 74-75 21.96822453735803 24.693473136124318 30.06378800552889 23.27451432098876 76-77 22.214077271169277 24.604778831029915 30.05179406059732 23.12934983720349 78-79 22.514234528215482 25.04779323575443 29.982112832854234 22.455859403175854 80-81 22.509739059738305 24.64374919272183 30.332858611833817 22.513653135706047 82-83 21.81323993261102 24.93420180796154 30.393368906573 22.859189352854443 84-85 21.4998311366183 24.78400025049932 30.117958341068245 23.59821027181413 86-87 21.780252198573947 24.810839886559847 30.075060164434543 23.333847750431662 88-89 21.276546967906 24.429584765219182 30.388093717744123 23.905774549130694 90-91 20.633090532326342 24.300490988479858 30.654880557448895 24.411537921744902 92-93 20.415861259549562 24.944574384391252 30.234680303930872 24.404884052128313 94-95 19.506394980189373 24.543706952562967 30.860462281526747 25.089435785720916 96-97 19.27395699254208 24.438922543409486 30.81768742933762 25.469433034710814 98-99 19.499349710417047 24.451223808091324 30.203407002998155 25.846019478493474 100 19.69924526149693 24.27665767928814 30.133625283348447 25.890471775866487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1174.0 1 747.5 2 998.0 3 1662.0 4 973.0 5 554.5 6 1407.0 7 2885.5 8 4788.5 9 5349.0 10 3272.0 11 1282.0 12 895.5 13 869.5 14 906.5 15 988.5 16 1059.5 17 1191.5 18 1438.0 19 1715.0 20 2035.5 21 2465.0 22 3095.0 23 3879.5 24 4843.0 25 6037.0 26 7329.0 27 8755.5 28 10527.0 29 12797.0 30 15198.0 31 17624.5 32 20450.0 33 22717.5 34 24362.0 35 26029.5 36 28012.5 37 29184.5 38 29717.5 39 29733.0 40 29522.0 41 28917.0 42 27747.5 43 27013.5 44 25795.0 45 24763.5 46 24564.5 47 24863.5 48 25793.0 49 25924.5 50 26827.5 51 26170.0 52 23078.5 53 22070.5 54 22445.0 55 23223.0 56 23995.0 57 23548.0 58 21015.0 59 17691.0 60 14628.5 61 11636.0 62 8668.5 63 6471.0 64 4715.5 65 3310.0 66 2435.5 67 1852.5 68 1345.5 69 1053.0 70 909.0 71 623.0 72 419.0 73 370.0 74 293.0 75 375.5 76 462.5 77 280.0 78 159.5 79 250.0 80 246.0 81 173.5 82 139.5 83 59.0 84 8.0 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08465506694683729 2 0.001230126468183897 3 1.1182967892580885E-4 4 2.236593578516177E-4 5 0.04361357478106545 6 1.1182967892580885E-4 7 0.005479654267364633 8 4.473187157032354E-4 9 3.354890367774265E-4 10-11 2.795741973145221E-4 12-13 0.0 14-15 5.591483946290442E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.795741973145221E-4 24-25 5.591483946290442E-5 26-27 0.001341956147109706 28-29 0.007492588488029191 30-31 0.03237469204902166 32-33 0.023484232574419856 34-35 0.026279974547565074 36-37 0.02326057321656824 38-39 0.02040891640396011 40-41 0.026559548744879598 42-43 0.019402449293627833 44-45 0.01822823766490684 46-47 0.01202169048452445 48-49 0.008778629795675993 50-51 0.006653865896085625 52-53 0.004920505872735588 54-55 0.0035226348861629782 56-57 0.002124763899590368 58-59 8.387225919435663E-4 60-61 3.9140387624033096E-4 62-63 0.001230126468183897 64-65 0.0027398271336823163 66-67 2.795741973145221E-4 68-69 6.150632340919485E-4 70-71 2.795741973145221E-4 72-73 3.354890367774265E-4 74-75 5.591483946290442E-4 76-77 2.795741973145221E-4 78-79 5.591483946290442E-5 80-81 9.505522708693752E-4 82-83 6.150632340919485E-4 84-85 3.354890367774265E-4 86-87 1.1182967892580885E-4 88-89 0.0 90-91 5.591483946290442E-5 92-93 5.591483946290442E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 894217.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.254245700836286 #Duplication Level Percentage of deduplicated Percentage of total 1 74.60338564945675 35.9993010124313 2 12.023535059020741 11.603732298612119 3 4.591110220359637 6.646216818385635 4 2.487040073585737 4.800409711145284 5 1.566351941347427 3.779156571588032 6 1.0431550655112194 3.0201996501150217 7 0.723969455242649 2.4454219981225567 8 0.5232523050286095 2.019931623230357 9 0.41271747241171786 1.7923833286904838 >10 1.9228945330536877 15.641185120027085 >50 0.06568508253271184 2.1788231706029086 >100 0.030172380628022456 2.83595777830058 >500 0.0032493332929780655 1.0702319131483844 >1k 0.0027851428225526276 2.3297367368472885 >5k 2.3209523521271894E-4 0.7762429248672511 >10k+ 4.641904704254379E-4 3.061069343885688 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15033 1.6811355632916845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12299 1.375393221108523 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6931 0.775091504634781 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2721 0.30428855635712587 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2526 0.2824817689665931 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2214 0.24759090914174076 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2039 0.22802071532972423 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1731 0.1935771742205751 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 1660 0.18563726701684266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1597 0.17859199724451671 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1463 0.16360682026845833 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 1419 0.15868631439572276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 1240 0.13866880186800296 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1146 0.12815681204897691 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1046 0.11697384415639603 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 979 0.10948125566836685 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 925 0.10344245300637318 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1182967892580883E-4 0.0 4 0.0 0.0 0.0 0.0010064671103322796 0.0 5 0.0 0.0 0.0 0.0010064671103322796 0.0 6 0.0 0.0 0.0 0.0011182967892580883 0.0 7 0.0 0.0 0.0 0.0011182967892580883 0.0 8 0.0 0.0 0.0 0.0011182967892580883 0.0 9 0.0 0.0 0.0 0.001453785826035515 0.0 10-11 0.0 0.0 0.0 0.001733360023350037 0.0 12-13 0.0 0.0 0.0 0.0019011045417387502 0.0 14-15 0.0 0.0 0.0 0.0019011045417387502 0.0 16-17 0.0 0.0 0.0 0.002683912294219412 0.0 18-19 0.0 0.0 0.0 0.003354890367774265 0.0 20-21 0.0 0.0 0.0 0.004081783280792022 0.0 22-23 0.0 0.0 0.0 0.007157099451251765 0.0 24-25 0.0 0.0 0.0 0.012133520163450259 0.0 26-27 0.0 0.0 0.0 0.01834006734383265 0.0 28-29 0.0 0.0 0.0 0.029746694594265148 0.0 30-31 0.0 0.0 0.0 0.05843100723873512 0.0 32-33 0.0 0.0 0.0 0.0974036503443795 0.0 34-35 0.0 0.0 0.0 0.14828615425562253 0.0 36-37 0.0 0.0 0.0 0.2364638560886228 0.0 38-39 0.0 0.0 0.0 0.379661759953121 0.0 40-41 0.0 0.0 0.0 0.5691012360534411 0.0 42-43 0.0 0.0 0.0 0.7308628666196236 0.0 44-45 0.0 0.0 0.0 0.8901083294099754 0.0 46-47 0.0 0.0 0.0 1.0959308534729266 0.0 48-49 0.0 0.0 0.0 1.3428507845411124 0.0 50-51 0.0 0.0 0.0 1.6113538436419796 0.0 52-53 0.0 0.0 0.0 1.8738740149203157 0.0 54-55 0.0 0.0 0.0 2.108157192269885 0.0 56-57 0.0 0.0 0.0 2.3818603314408024 0.0 58-59 0.0 0.0 0.0 2.719194557920505 0.0 60-61 0.0 0.0 0.0 3.080907654406033 0.0 62-63 0.0 0.0 0.0 3.423945194510952 0.0 64-65 0.0 0.0 0.0 3.7571417228703994 0.0 66-67 0.0 0.0 0.0 4.114605291556748 0.0 68-69 0.0 0.0 0.0 4.516017924060939 0.0 70-71 0.0 0.0 0.0 4.981956281305321 0.0 72-73 0.0 0.0 0.0 5.455107652840418 0.0 74-75 0.0 0.0 0.0 5.8894541257882596 0.0 76-77 0.0 0.0 0.0 6.332523313692315 0.0 78-79 0.0 0.0 0.0 6.84453549865413 0.0 80-81 0.0 0.0 0.0 7.386350293049674 1.1182967892580883E-4 82-83 0.0 0.0 0.0 7.960875268531017 1.1182967892580883E-4 84-85 0.0 0.0 0.0 8.516668772792286 1.1182967892580883E-4 86-87 0.0 0.0 0.0 9.06826866409384 1.1182967892580883E-4 88 0.0 0.0 0.0 9.515028231402445 1.1182967892580883E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6775 0.0 51.04608 1 TATAACG 65 2.7777824E-6 43.399227 2 GTATCAA 12085 0.0 41.42661 1 ACGTGCG 155 0.0 39.41279 9 TATCAAC 14840 0.0 33.51937 2 ATCAACG 15260 0.0 32.535202 3 AACACGT 190 0.0 32.157932 6 TCAACGC 15425 0.0 32.156696 4 CAACGCA 15620 0.0 31.755253 5 AACGCAG 15860 0.0 31.293858 6 TCTGTCG 605 0.0 31.069206 8 CTGTCGC 620 0.0 30.31753 9 GTCCTAA 425 0.0 29.892294 1 ACGCAGA 17545 0.0 28.154493 7 CGCAGAG 17565 0.0 27.9572 8 TACAGCG 85 6.814848E-4 27.64709 7 CCCGTAC 140 6.3036714E-7 26.887249 1 GCGAGCT 175 5.5606506E-9 26.852669 9 GCAGAGT 18550 0.0 26.422014 9 GTCTAAC 180 7.405106E-9 26.140379 1 >>END_MODULE