FastQCFastQC Report
Wed 25 May 2016
SRR1033193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343392
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.035.0930371
GGTATCA17400.034.930571
TCAACGC29100.033.253664
ATCAACG29650.032.802253
CAACGCA30550.031.828425
AACGCAG32450.030.099296
GTACATG69300.028.1470151
TATCAAC34900.028.019192
TACATGG67850.027.5079562
ACATGGG70250.025.749713
ACGCAGA38800.024.4470967
CGCAGAG40100.023.5374438
CATGGGG47000.020.8888054
CCGATCG1354.1036066E-420.8701299
GAGTACA49450.019.9133911
CTGTCGC5200.018.0606869
AATACCG3209.507858E-917.6151855
AGTACAT50950.017.439552
GCAGAGT51000.017.4020029
TCTGTCG5700.017.3002388
Produced by FastQC (version 0.11.5)