##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033193_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2343392
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.540897980363507	33.0	31.0	34.0	30.0	34.0
2	31.60240753574306	34.0	31.0	34.0	30.0	34.0
3	31.792147024484166	34.0	31.0	34.0	30.0	34.0
4	35.14249131173956	37.0	35.0	37.0	33.0	37.0
5	35.25102927721866	37.0	35.0	37.0	33.0	37.0
6	35.30230878999331	37.0	36.0	37.0	33.0	37.0
7	35.25420885622209	37.0	36.0	37.0	33.0	37.0
8	35.273670815638184	37.0	36.0	37.0	33.0	37.0
9	36.918293652961175	39.0	38.0	39.0	34.0	39.0
10-11	36.90383853832394	39.0	38.0	39.0	34.0	39.0
12-13	36.85007651302044	39.0	38.0	39.0	34.0	39.0
14-15	38.290067133454414	41.0	39.0	41.0	34.0	41.0
16-17	38.29360153998989	41.0	39.0	41.0	34.0	41.0
18-19	38.27007666664391	41.0	39.0	41.0	34.0	41.0
20-21	38.29762839507859	41.0	39.0	41.0	34.0	41.0
22-23	38.256330140241154	41.0	39.0	41.0	34.0	41.0
24-25	38.24636915206675	41.0	39.0	41.0	34.0	41.0
26-27	38.20491642030015	40.0	39.0	41.0	33.5	41.0
28-29	38.1669697174011	40.0	38.5	41.0	33.0	41.0
30-31	38.161955191448975	40.0	38.0	41.0	33.0	41.0
32-33	38.232763233808086	41.0	39.0	41.0	33.5	41.0
34-35	38.20087697662192	41.0	39.0	41.0	33.0	41.0
36-37	38.116157902732446	41.0	38.0	41.0	33.0	41.0
38-39	38.03310286968633	41.0	38.0	41.0	33.0	41.0
40-41	37.96572212416872	40.5	38.0	41.0	33.0	41.0
42-43	37.84364395713564	40.0	38.0	41.0	33.0	41.0
44-45	37.70316511279376	40.0	38.0	41.0	32.5	41.0
46-47	37.568935116275895	40.0	38.0	41.0	32.0	41.0
48-49	37.44497036774044	40.0	37.5	41.0	32.0	41.0
50-51	37.31144127828379	40.0	37.0	41.0	31.5	41.0
52-53	37.15142579645232	40.0	37.0	41.0	31.0	41.0
54-55	36.99054703609127	40.0	36.5	41.0	31.0	41.0
56-57	36.82223290000137	40.0	36.0	41.0	31.0	41.0
58-59	36.61548772036433	39.5	35.5	41.0	30.5	41.0
60-61	36.386185921945625	39.0	35.0	41.0	30.5	41.0
62-63	36.11413049972006	39.0	35.0	41.0	30.0	41.0
64-65	35.837180463191814	38.5	35.0	41.0	30.0	41.0
66-67	35.53705141948082	38.0	35.0	40.0	29.5	41.0
68-69	35.224355336196425	37.0	35.0	40.0	29.0	41.0
70-71	34.87628958364627	37.0	34.5	39.5	29.0	41.0
72-73	34.511561659338255	36.0	34.0	39.0	29.0	41.0
74-75	34.146924628913986	36.0	34.0	39.0	28.0	40.5
76-77	33.82254654791004	35.0	34.0	38.5	28.0	40.0
78-79	33.462917642460155	35.0	34.0	37.0	28.0	39.0
80-81	33.11364253185127	35.0	34.0	37.0	27.0	39.0
82-83	32.907454023910645	35.0	34.0	36.0	27.0	39.0
84-85	32.64708145286832	35.0	34.0	36.0	27.0	37.0
86-87	32.40262768670372	35.0	34.0	36.0	27.0	37.0
88-89	32.05928798937609	35.0	33.0	35.0	26.0	37.0
90-91	31.88128106607857	35.0	33.0	35.0	26.0	36.0
92-93	31.71251416749737	35.0	33.0	35.0	25.5	36.0
94-95	31.55211974778441	35.0	33.0	35.0	25.0	36.0
96-97	31.383740535087597	35.0	33.0	35.0	25.0	36.0
98-99	31.197868090357908	35.0	33.0	35.0	24.0	35.0
100	31.035897109830536	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15672252547848586
1101	2	0.13289902797299646
1101	3	0.18409315222167777
1101	4	0.09870201508054777
1101	5	0.09399157386146584
1101	6	0.0901751901854837
1101	7	0.14730103890219226
1101	8	0.09487654722489225
1101	9	0.13555206215851
1101	10-11	0.1861119254872392
1101	12-13	0.1611350705294825
1101	14-15	0.16535760747867556
1101	16-17	0.14587609378052946
1101	18-19	0.13185481568101665
1101	20-21	0.11655901264843038
1101	22-23	0.16557375764786997
1101	24-25	0.17368767992292788
1101	26-27	0.18066285265634008
1101	28-29	0.22817127535075343
1101	30-31	0.2731909313277825
1101	32-33	0.27875244354741113
1101	34-35	0.23784976328725804
1101	36-37	0.26711036177456293
1101	38-39	0.24224314704083127
1101	40-41	0.2894221644640069
1101	42-43	0.22644895323201553
1101	44-45	0.1837970274007077
1101	46-47	0.27109124995178746
1101	48-49	0.18910279636605054
1101	50-51	0.20826287301228774
1101	52-53	0.20629322550379925
1101	54-55	0.23103404167769526
1101	56-57	0.19367500511423685
1101	58-59	0.24019194832668944
1101	60-61	0.2180108621612007
1101	62-63	0.14172358614308678
1101	64-65	0.16245828647067384
1101	66-67	0.18949117932380233
1101	68-69	0.2409690849473023
1101	70-71	0.24420858693447656
1101	72-73	0.2397939512026781
1101	74-75	0.24903917638142303
1101	76-77	0.24747781292078486
1101	78-79	0.12357075122972105
1101	80-81	0.14485085055942903
1101	82-83	0.205993546784903
1101	84-85	0.08766449546025257
1101	86-87	0.2120336379722474
1101	88-89	0.3889433586696356
1101	90-91	0.408536632223786
1101	92-93	0.4209740636109629
1101	94-95	0.4781438965189828
1101	96-97	0.5644908412087766
1101	98-99	0.5220474877930172
1101	100	0.5488465052462423
1102	1	0.16403846718593584
1102	2	0.12958841052882875
1102	3	0.11323524347309188
1102	4	0.1954327579894226
1102	5	0.1413277573913021
1102	6	0.1424617934766772
1102	7	0.11266569437820806
1102	8	0.2315779313405102
1102	9	0.2567940575982348
1102	10-11	0.21049579687790754
1102	12-13	0.19790199902576688
1102	14-15	0.21889481226430263
1102	16-17	0.2111842999787399
1102	18-19	0.19292192074158265
1102	20-21	0.1729029761193175
1102	22-23	0.22968881556975873
1102	24-25	0.2945292423363526
1102	26-27	0.3283337757317426
1102	28-29	0.2821257117679892
1102	30-31	0.34720916467313856
1102	32-33	0.3469184006469632
1102	34-35	0.3622996462387533
1102	36-37	0.3048139367662728
1102	38-39	0.2773372724095182
1102	40-41	0.3036947167655697
1102	42-43	0.3253620735292131
1102	44-45	0.23043070120896658
1102	46-47	0.23941857306222403
1102	48-49	0.28008309836123857
1102	50-51	0.2478383493943923
1102	52-53	0.24300697498331658
1102	54-55	0.24519849644516967
1102	56-57	0.16859349460352036
1102	58-59	0.20422494158270155
1102	60-61	0.14520302299761312
1102	62-63	0.13604043905061758
1102	64-65	0.20156862040607137
1102	66-67	0.08599308348049561
1102	68-69	0.04009638249205949
1102	70-71	0.10516315127954812
1102	72-73	0.1279507257273238
1102	74-75	0.02940536410891781
1102	76-77	-0.04227899396851598
1102	78-79	0.03574257332871156
1102	80-81	0.036350646938466014
1102	82-83	0.06193467174873746
1102	84-85	0.10194181896332566
1102	86-87	0.16898984599623645
1102	88-89	0.19321346473643075
1102	90-91	0.18806969858852085
1102	92-93	0.1733549180118814
1102	94-95	0.12659471224037233
1102	96-97	0.15500281995625542
1102	98-99	0.1815823513103112
1102	100	0.24889866044269127
1103	1	-0.11230502068004
1103	2	-0.14467578755271404
1103	3	-0.05468979403054419
1103	4	-0.07417485871771134
1103	5	-0.0992902409342733
1103	6	-0.1147149538063914
1103	7	-0.07993914884048792
1103	8	-0.08425609841131632
1103	9	-0.03352663686112578
1103	10-11	-0.050467256621288925
1103	12-13	-0.06563390636598143
1103	14-15	-0.06887537048213943
1103	16-17	-0.13730337376810553
1103	18-19	-0.07964333737911744
1103	20-21	-0.18294765243320654
1103	22-23	-0.1530734640429543
1103	24-25	-0.2010585958021025
1103	26-27	-0.13159219278588097
1103	28-29	-0.12814833375138335
1103	30-31	-0.11121750857562063
1103	32-33	-0.10444853968454737
1103	34-35	-0.16078196286251512
1103	36-37	-0.0845920666681721
1103	38-39	-0.05966240084763541
1103	40-41	-0.05059332721426557
1103	42-43	-0.11468075727098892
1103	44-45	-0.18901689274424172
1103	46-47	-0.07201335499351558
1103	48-49	-0.04839273367348795
1103	50-51	-0.1267540367890092
1103	52-53	-0.16663479149860905
1103	54-55	-0.06473881219324795
1103	56-57	-0.08458946145471202
1103	58-59	-0.03959242551236031
1103	60-61	-0.15195121596409678
1103	62-63	-0.18899887824859718
1103	64-65	-0.19218748358751014
1103	66-67	-0.19493179428848606
1103	68-69	-0.298804992652272
1103	70-71	-0.274494268441984
1103	72-73	-0.19359299107591
1103	74-75	-0.2230297390861793
1103	76-77	-0.28851566554813246
1103	78-79	-0.36823716200297696
1103	80-81	-0.2813120658470254
1103	82-83	-0.3088265389359748
1103	84-85	-0.3405428454243662
1103	86-87	-0.29282115192778235
1103	88-89	-0.21147100171631905
1103	90-91	-0.20409652141625045
1103	92-93	-0.20617266417518465
1103	94-95	-0.25143843945265587
1103	96-97	-0.2595079721452791
1103	98-99	-0.2232416349957731
1103	100	-0.2247327957393388
1104	1	0.1114872836593932
1104	2	0.014438233277552115
1104	3	0.11868077937415933
1104	4	0.11980338485616926
1104	5	0.13584162313819093
1104	6	0.1217546604026083
1104	7	0.07009155586678162
1104	8	0.05485950741364576
1104	9	0.07817230398624986
1104	10-11	0.04869064190215511
1104	12-13	0.07819564352291763
1104	14-15	-0.015448038563256716
1104	16-17	0.0481720488621491
1104	18-19	0.07926908965497859
1104	20-21	0.08893613933427957
1104	22-23	0.005882787729440508
1104	24-25	0.07798045185712255
1104	26-27	0.16581138320319866
1104	28-29	0.18778881536539416
1104	30-31	0.11922615846665963
1104	32-33	0.11968970618282526
1104	34-35	0.15082466299966768
1104	36-37	0.17762522040158046
1104	38-39	0.10008557221256353
1104	40-41	0.09855307644554046
1104	42-43	0.07474957408602734
1104	44-45	0.06793466941594772
1104	46-47	0.11047623343558399
1104	48-49	0.0027494120477840056
1104	50-51	0.06623134820857501
1104	52-53	-0.0067196909728792775
1104	54-55	0.010800245783052276
1104	56-57	0.002630532705644839
1104	58-59	-0.04076327643721811
1104	60-61	-0.020702424568206368
1104	62-63	-0.005806678832897205
1104	64-65	-0.1056453120461569
1104	66-67	0.03538272672812326
1104	68-69	-0.007138531414064175
1104	70-71	0.005164899264151757
1104	72-73	0.030062178353269076
1104	74-75	-0.01826978817718583
1104	76-77	-0.10769210193588918
1104	78-79	-0.018026419792683157
1104	80-81	-0.06208442755669097
1104	82-83	-0.013822276067159578
1104	84-85	-0.04032777552116329
1104	86-87	-0.016557168868963856
1104	88-89	0.038234048664783415
1104	90-91	0.12484904259808616
1104	92-93	0.18211570406364075
1104	94-95	0.18039697345581018
1104	96-97	0.1988455160025957
1104	98-99	0.09937773674373318
1104	100	0.09585922534620295
1105	1	0.13608302130321093
1105	2	0.13349219915733457
1105	3	0.15076657232533108
1105	4	0.14443288412108046
1105	5	0.0733740735213857
1105	6	0.11091911934133947
1105	7	0.09958740054287318
1105	8	0.10828555386082428
1105	9	0.014213777713628417
1105	10-11	0.051294473872602
1105	12-13	0.10660807513359316
1105	14-15	0.09949313599913268
1105	16-17	0.22260714994691
1105	18-19	0.11825644553119474
1105	20-21	0.15543009283069154
1105	22-23	0.258754685208352
1105	24-25	0.2638517511471079
1105	26-27	0.3231010901752498
1105	28-29	0.32082154686036546
1105	30-31	0.365198476405574
1105	32-33	0.33793231772090593
1105	34-35	0.2692968800063227
1105	36-37	0.36270695614029336
1105	38-39	0.3123188355720714
1105	40-41	0.30872908467949856
1105	42-43	0.3092653751417558
1105	44-45	0.2719401023042778
1105	46-47	0.24109941654468514
1105	48-49	0.2973977014619251
1105	50-51	0.25829852202240033
1105	52-53	0.258258965159456
1105	54-55	0.3393069895566754
1105	56-57	0.33367389373407974
1105	58-59	0.2677830327829085
1105	60-61	0.24338370829108413
1105	62-63	0.30040536248286287
1105	64-65	0.35700710668750446
1105	66-67	0.34723159243461765
1105	68-69	0.22816229344493166
1105	70-71	0.3271397830862668
1105	72-73	0.3616754320197586
1105	74-75	0.4250258502621236
1105	76-77	0.4480647745482358
1105	78-79	0.38464742126253526
1105	80-81	0.3168570213298878
1105	82-83	0.30901405799553316
1105	84-85	0.32343535647125776
1105	86-87	0.416931952748854
1105	88-89	0.38008518336377506
1105	90-91	0.35448756758724187
1105	92-93	0.313109119048967
1105	94-95	0.3038826589692185
1105	96-97	0.3201574114998884
1105	98-99	0.34393017136211057
1105	100	0.384483357306717
1106	1	0.09584732388089989
1106	2	0.0442813267870541
1106	3	0.09202389315937154
1106	4	0.023086881185577113
1106	5	0.031128929926147464
1106	6	0.07589942758593082
1106	7	-0.05227148515957225
1106	8	0.062387121897515385
1106	9	-0.00624887265091445
1106	10-11	0.016073470645281418
1106	12-13	-0.0022682475023714233
1106	14-15	3.995753982550809E-4
1106	16-17	-0.008499587071355563
1106	18-19	0.01844651791162022
1106	20-21	0.02740108263446217
1106	22-23	0.06118950764904696
1106	24-25	0.06362671085145877
1106	26-27	0.03990670858707546
1106	28-29	0.034946917091716045
1106	30-31	0.03946667648008173
1106	32-33	-0.02198199504532994
1106	34-35	0.01388656645568176
1106	36-37	-0.03505570593024032
1106	38-39	-0.07433371775274367
1106	40-41	-0.08706244251293782
1106	42-43	-0.07803046609423347
1106	44-45	-0.09263193010032467
1106	46-47	-0.07175186759655361
1106	48-49	-0.07989626548047113
1106	50-51	-0.07893258604863718
1106	52-53	-0.09091963901208544
1106	54-55	-0.1266613248764159
1106	56-57	-0.1437574185749284
1106	58-59	-0.15588410934957864
1106	60-61	-0.23276511362200836
1106	62-63	-0.12582117424611994
1106	64-65	-0.14351028338867877
1106	66-67	-0.06292258488890212
1106	68-69	-0.13086769125056463
1106	70-71	-0.09047475809699534
1106	72-73	-0.0722956975222786
1106	74-75	-0.028366145903916617
1106	76-77	-0.04650035765449445
1106	78-79	-0.03781018451748963
1106	80-81	-0.03923946043426696
1106	82-83	-0.06352843294066446
1106	84-85	-0.09729186277407109
1106	86-87	-0.09561164041870995
1106	88-89	0.014045815861159383
1106	90-91	0.02385917564109974
1106	92-93	-0.010199647809251644
1106	94-95	-0.05318923919994134
1106	96-97	-0.06101054021404195
1106	98-99	-0.009329356936223832
1106	100	0.10342361103004194
1107	1	0.20472830841422152
1107	2	0.17872261035408243
1107	3	0.18014199927923613
1107	4	0.10629860073316166
1107	5	0.13426423061380177
1107	6	0.12478395351269
1107	7	0.15116243412519736
1107	8	0.1466362881133989
1107	9	0.19555509760138534
1107	10-11	0.23789134054252514
1107	12-13	0.15871338005979396
1107	14-15	0.18042121940909084
1107	16-17	0.1853326806009079
1107	18-19	0.24426013035344596
1107	20-21	0.16127272722903996
1107	22-23	0.1778862048132126
1107	24-25	0.14520864351828067
1107	26-27	0.2363859408441158
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2216	4	-0.06176631853806214
2216	5	-1.2047965062578214E-4
2216	6	-0.010276192492490566
2216	7	0.025711230822984987
2216	8	-0.015511849732043004
2216	9	-0.07655889526699156
2216	10-11	-0.03356988819774642
2216	12-13	-0.09527867061049733
2216	14-15	-0.09266625174829102
2216	16-17	-0.06541140682082869
2216	18-19	-0.10819607140251009
2216	20-21	-0.14561143152931066
2216	22-23	-0.08528517423729909
2216	24-25	-0.16068732587657308
2216	26-27	-0.2769176468239536
2216	28-29	-0.14878040618854982
2216	30-31	-0.1589364616863591
2216	32-33	-0.10478881980159116
2216	34-35	-0.16294123064400878
2216	36-37	-0.1739330133041861
2216	38-39	-0.08742777287389458
2216	40-41	-0.1612141096638524
2216	42-43	-0.17073482657077932
2216	44-45	-0.16988469149376328
2216	46-47	-0.015751278322269968
2216	48-49	0.09335050308536808
2216	50-51	0.07693812288304969
2216	52-53	0.01931952515799651
2216	54-55	-0.022360286395375795
2216	56-57	-0.03745857940442221
2216	58-59	-0.04242940457650235
2216	60-61	-0.031264647155744285
2216	62-63	0.09246039673666928
2216	64-65	0.12106385104449657
2216	66-67	0.1448889632531305
2216	68-69	0.1272698726364183
2216	70-71	0.013171006353623227
2216	72-73	0.07630042286737648
2216	74-75	0.08266488750650325
2216	76-77	0.07223036901670099
2216	78-79	-0.007424516240106982
2216	80-81	-0.016581698073160567
2216	82-83	-0.05750771183029002
2216	84-85	-0.09286962476751626
2216	86-87	-0.06777915363525722
2216	88-89	0.014463261035416508
2216	90-91	-0.0040002472071165585
2216	92-93	0.02934587822528556
2216	94-95	0.036163751418133216
2216	96-97	-0.04217099694757209
2216	98-99	-0.03400132417788271
2216	100	0.024415537359523398
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28474.0
3	4699.0
4	3076.0
5	2548.0
6	2191.0
7	2364.0
8	2552.0
9	3034.0
10	3311.0
11	3660.0
12	4026.0
13	4417.0
14	4821.0
15	5087.0
16	5139.0
17	5249.0
18	5070.0
19	5177.0
20	5370.0
21	5546.0
22	6189.0
23	8253.0
24	12029.0
25	16303.0
26	17442.0
27	16168.0
28	17200.0
29	21233.0
30	26075.0
31	31460.0
32	41386.0
33	56640.0
34	79050.0
35	121377.0
36	231049.0
37	481786.0
38	743460.0
39	309729.0
40	752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.835390629348474	19.097101802970556	12.90825872691697	24.159248840764004
2	15.659239365649563	19.52760810373755	40.48126142068761	24.33189110992528
3	18.663372995454477	24.65961957670489	29.31048544642838	27.36652198141225
4	12.207040755166755	16.111456424740318	38.403637860110415	33.27786495998251
5	12.993581355834518	37.816209477906156	35.541272927922066	13.648936238337258
6	31.789440620561564	37.488861234021634	17.542532161586365	13.179165983830437
7	28.262569188683994	31.88031051155883	21.964211793123166	17.892908506634004
8	25.576911996619767	36.61221449957	19.820062440381815	17.990811063428417
9	25.199180359004803	16.862905611109287	20.554704548956305	37.3832094809296
10-11	24.26180179199652	26.662910665868516	28.55779172762165	20.517495814513314
12-13	24.782609066783213	23.90655796337538	28.670165863285497	22.640667106555913
14-15	21.992516783672393	26.030500092510533	25.485530904812748	26.49145221900433
16-17	21.977741266282123	29.39697060815115	26.02139059572662	22.603897529840108
18-19	21.869101318658384	28.406971639807004	27.875632829474128	21.84829421206048
20-21	22.95457159802385	27.70530046753076	27.425832018867695	21.914295915577693
22-23	22.556248176774403	27.43663497481788	27.52685996752498	22.480256880882738
24-25	22.441743432931	27.6307117984861	27.423141966155402	22.504402802427496
26-27	22.236022485323524	27.569831287515463	27.53545155990278	22.658694667258228
28-29	22.18892615193863	27.581075587319848	27.68265681869351	22.547341442048012
30-31	22.428819163606768	28.034850216031902	27.23623855658337	22.30009206377796
32-33	22.0866574783606	27.713285582028252	27.753407946703838	22.446648992907306
34-35	22.273134379000556	27.475215356502584	27.74341468015716	22.508235584339705
36-37	22.592855690304418	27.798420555440917	27.295425781917665	22.313297972336997
38-39	22.34338059384745	27.995052658331897	27.384402515240115	22.27716423258054
40-41	22.077669894625792	27.743534088178723	27.67834470608871	22.50045131110678
42-43	22.161180250994743	27.58618408656722	27.687562694806005	22.565072967632034
44-45	22.193410499056412	27.796453711235408	27.663621519796582	22.346514269911598
46-47	22.23626759827057	27.962691175610775	27.50197486427742	22.299066361841227
48-49	22.183070191572778	27.759275752393965	27.56235151733441	22.495302538698844
50-51	22.219685376072977	27.61722860891022	27.611744753636202	22.5513412613806
52-53	22.19244353477193	27.716954334477634	27.62622417433847	22.46437795641197
54-55	22.345030069548834	28.059556590176104	27.412930599459955	22.182482740815104
56-57	22.153189230341177	27.825644255766598	27.434362469722494	22.586804044169735
58-59	22.004718078185416	27.461959001027168	27.840032569078065	22.693290351709344
60-61	22.37936264351531	27.839238954911526	27.41515825518157	22.36624014639159
62-63	22.14206080882847	27.870538795793586	27.53957780122497	22.44782259415297
64-65	22.196658013764132	27.618007994582083	27.59618014822452	22.589153843429262
66-67	22.04009435220342	27.66162508974875	27.516554843018376	22.78172571502946
68-69	22.449418965151565	27.770290771337574	27.44308393351481	22.337206329996043
70-71	22.4319038501012	27.558195755547317	27.49565684208999	22.514243552261494
72-73	22.13604210727557	27.683148213581593	27.61687551315468	22.56393416598815
74-75	22.25033024436696	27.75642293554863	27.46916207866505	22.524084741419355
76-77	22.30359078835095	27.69383806832669	27.38549290524473	22.617078238077628
78-79	22.44060012469525	27.7749067239417	27.449491382499907	22.335001768863144
80-81	22.264365846679667	27.61696651680028	27.59151106586646	22.5271565706536
82-83	22.164623887616607	27.655728341076212	27.66191610206904	22.517731669238145
84-85	22.20728612630859	27.580247909319976	27.500297121622282	22.712168842749154
86-87	22.338995491468356	27.69787503707323	27.500016002833565	22.463113468624847
88-89	22.157399544764925	27.67841273902632	27.51104816545073	22.653139550758024
90-91	22.117529315135073	27.54207979349848	27.606675920650453	22.733714970715994
92-93	22.264156694915727	27.732537396481057	27.463041444501734	22.54026446410148
94-95	22.05377131094937	27.518453570409037	27.677822875773504	22.74995224286809
96-97	22.120839889582427	27.461660028319134	27.665242696941277	22.752257385157158
98-99	22.12819039577574	27.586040373207382	27.52980729008265	22.75596194093423
100	22.33313071892058	27.4058744443128	27.42879734609461	22.83219749067201
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	276.0
1	262.5
2	260.5
3	232.5
4	176.0
5	149.5
6	139.5
7	138.5
8	145.5
9	167.5
10	169.0
11	164.0
12	174.5
13	179.0
14	203.0
15	250.0
16	335.0
17	461.0
18	654.5
19	970.0
20	1424.5
21	2090.5
22	3151.0
23	4883.0
24	7031.0
25	9527.5
26	13040.0
27	17558.5
28	23045.0
29	28943.5
30	34445.0
31	40530.5
32	46849.5
33	52580.5
34	58416.5
35	64338.5
36	69221.0
37	73476.0
38	77217.5
39	79227.0
40	80829.0
41	82115.5
42	82070.5
43	82158.5
44	81614.0
45	80720.0
46	80510.5
47	78639.0
48	76711.5
49	75610.0
50	73672.0
51	71775.5
52	68237.5
53	64723.0
54	62464.5
55	59854.5
56	56570.5
57	52176.5
58	47743.0
59	43930.5
60	39848.0
61	34717.0
62	30107.5
63	25832.0
64	21745.0
65	18221.5
66	14671.0
67	11045.0
68	8114.0
69	6219.5
70	4648.5
71	3461.5
72	2701.5
73	2055.0
74	1578.5
75	1152.0
76	783.0
77	596.0
78	432.5
79	289.0
80	210.0
81	126.0
82	82.5
83	55.5
84	48.0
85	37.5
86	24.0
87	22.5
88	16.0
89	12.0
90	11.0
91	8.5
92	5.5
93	4.0
94	2.5
95	2.0
96	2.0
97	3.5
98	3.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3329361882262976
2	0.11918620529557154
3	0.06516195327115566
4	0.03908863732572271
5	0.04254516529884885
6	0.009558793407163632
7	0.0069130559462522705
8	0.015234326992667038
9	0.017709371714164767
10-11	0.020248426212942604
12-13	0.016130463874588632
14-15	0.017730708306591472
16-17	0.019053577037047152
18-19	0.018882884297633514
20-21	0.023534261446655106
22-23	0.015383683139653971
24-25	0.024153022627029536
26-27	0.018840211112780107
28-29	0.01875486474307329
30-31	0.019245606368887492
32-33	0.023854310333055673
34-35	0.015298336769947154
36-37	0.009665476369297156
38-39	0.013463389821250565
40-41	0.009345427482896587
42-43	0.008342607638841475
44-45	0.008961368819215906
46-47	0.00823592467670795
48-49	0.004480684409607953
50-51	0.006400977728011361
52-53	0.007681173273613634
54-55	0.003179152271578976
56-57	0.002901776570031817
58-59	0.0027737570154715897
60-61	0.0037552402670999988
62-63	0.0027524204230448854
64-65	0.002347025166937499
66-67	0.001898956725976704
68-69	0.0031151424942988627
70-71	0.0021763324275238627
72-73	0.0018776201335499994
74-75	0.0021976690199505674
76-77	0.0032644986412857947
78-79	0.003371181603419317
80-81	0.0035205377504062487
82-83	0.002411034944217613
84-85	0.003221825456432385
86-87	0.0024323715366443175
88-89	0.5993875544509838
90-91	0.6104399093280168
92-93	0.6071967472791577
94-95	0.5930292499078259
96-97	0.591941083694064
98-99	0.5889966339391788
100	0.5907675711105952
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2343392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.67648070332363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4354420487708	56.74135006477808
2	11.877627153870888	16.551825183759156
3	3.2261150398826253	6.743530269692517
4	1.2312642112614303	3.4316062822660025
5	0.639487897558999	2.22786330771393
6	0.38981088533900665	1.6296390378161278
7	0.23375121859264175	1.1400873590154013
8	0.18435330596400598	1.0276071652475984
9	0.12312790961344984	0.7721207476399903
>10	0.621676280784883	7.596905286365351
>50	0.030152164940381216	1.4039957180762375
>100	0.007191883420981882	0.7334695776295367
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.267318485340908E-5	0.0
8	0.0	0.0	0.0	4.267318485340908E-5	0.0
9	0.0	0.0	0.0	4.267318485340908E-5	0.0
10-11	0.0	0.0	0.0	8.534636970681816E-5	0.0
12-13	0.0	0.0	0.0	8.534636970681816E-5	0.0
14-15	0.0	0.0	0.0	8.534636970681816E-5	0.0
16-17	0.0	0.0	0.0	2.347025166937499E-4	0.0
18-19	0.0	0.0	0.0	2.5603910912045443E-4	0.0
20-21	0.0	0.0	0.0	2.987122939738635E-4	0.0
22-23	0.0	0.0	0.0	4.0539525610738624E-4	0.0
24-25	0.0	0.0	0.0	4.907416258142043E-4	0.0
26-27	0.0	0.0	0.0	7.041075500812498E-4	0.0
28-29	0.0	0.0	0.0	0.0014508882850159084	0.0
30-31	0.0	0.0	0.0	0.004672713741448294	0.0
32-33	0.0	0.0	0.0	0.01086032554519261	0.0
34-35	0.0	0.0	0.0	0.02026976280536931	0.0
36-37	0.0	0.0	0.0	0.03753106607857328	0.0
38-39	0.0	0.0	0.0	0.06689021725771872	0.0
40-41	0.0	0.0	0.0	0.1025223266103153	0.0
42-43	0.0	0.0	0.0	0.13834646529475222	0.0
44-45	0.0	0.0	0.0	0.1800595034889596	0.0
46-47	0.0	0.0	0.0	0.2309046032417965	0.0
48-49	0.0	0.0	0.0	0.28902548101213965	0.0
50-51	0.0	0.0	0.0	0.3535900096953476	0.0
52-53	0.0	0.0	0.0	0.4217177493138152	0.0
54-55	0.0	0.0	0.0	0.4919578115825265	0.0
56-57	0.0	0.0	0.0	0.5733995848752578	0.0
58-59	0.0	0.0	0.0	0.6691582116863077	0.0
60-61	0.0	0.0	0.0	0.7707203916374212	0.0
62-63	0.0	4.267318485340908E-5	0.0	0.8778727588043316	0.0
64-65	0.0	4.267318485340908E-5	0.0	0.9860919555925769	0.0
66-67	0.0	4.267318485340908E-5	0.0	1.1087560254537014	0.0
68-69	0.0	4.267318485340908E-5	0.0	1.2507937212382734	0.0
70-71	0.0	4.267318485340908E-5	0.0	1.40652950936079	0.0
72-73	0.0	4.267318485340908E-5	0.0	1.570501222160014	0.0
74-75	0.0	4.267318485340908E-5	0.0	1.7368412967186027	0.0
76-77	0.0	4.267318485340908E-5	0.0	1.9120787303191271	0.0
78-79	0.0	4.267318485340908E-5	0.0	2.1170593737624777	0.0
80-81	0.0	4.267318485340908E-5	0.0	2.3409442380958883	0.0
82-83	0.0	4.267318485340908E-5	0.0	2.5790179363930577	0.0
84-85	0.0	4.267318485340908E-5	0.0	2.8121842184320847	0.0
86-87	0.0	4.267318485340908E-5	0.0	3.053010337152299	0.0
88	0.0	4.267318485340908E-5	0.0	3.2542144037361225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2900	0.0	35.093037	1
GGTATCA	1740	0.0	34.93057	1
TCAACGC	2910	0.0	33.25366	4
ATCAACG	2965	0.0	32.80225	3
CAACGCA	3055	0.0	31.82842	5
AACGCAG	3245	0.0	30.09929	6
GTACATG	6930	0.0	28.147015	1
TATCAAC	3490	0.0	28.01919	2
TACATGG	6785	0.0	27.507956	2
ACATGGG	7025	0.0	25.74971	3
ACGCAGA	3880	0.0	24.447096	7
CGCAGAG	4010	0.0	23.537443	8
CATGGGG	4700	0.0	20.888805	4
CCGATCG	135	4.1036066E-4	20.870129	9
GAGTACA	4945	0.0	19.913391	1
CTGTCGC	520	0.0	18.060686	9
AATACCG	320	9.507858E-9	17.615185	5
AGTACAT	5095	0.0	17.43955	2
GCAGAGT	5100	0.0	17.402002	9
TCTGTCG	570	0.0	17.300238	8
>>END_MODULE