FastQCFastQC Report
Wed 25 May 2016
SRR1033193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343392
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.1711194712621704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.14457675028334993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.051.0987551
GTATCAA58400.042.3026121
TCAACGC66050.036.9432374
ATCAACG66250.036.7607463
CAACGCA67500.036.1504175
AACGCAG69950.035.005976
TATCAAC72600.033.8052062
ACGCAGA82100.029.7109367
CGCAGAG84050.028.8520268
TAGCGCG1258.0081245E-626.3286424
GTACATG72550.025.425491
GCAGAGT97300.025.1162539
TACATGG71550.024.9045832
ACATGGG73450.023.2996833
GAGTACT61000.020.33898212-13
AGAGTAC88500.020.23233810-11
TATACCG1405.2004715E-420.1499025
CAGAGTA97000.019.9855710-11
GTATAAC6200.018.9744421
CTAGTAC4600.018.3973433
Produced by FastQC (version 0.11.5)