##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033193_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2343392 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74727830427005 34.0 31.0 34.0 31.0 34.0 2 32.84296609359424 34.0 31.0 34.0 31.0 34.0 3 32.937457753546994 34.0 31.0 34.0 31.0 34.0 4 36.35685493506848 37.0 37.0 37.0 35.0 37.0 5 36.29289679234204 37.0 37.0 37.0 35.0 37.0 6 36.3776781690814 37.0 37.0 37.0 35.0 37.0 7 36.349925236580134 37.0 37.0 37.0 35.0 37.0 8 36.350878128797916 37.0 37.0 37.0 35.0 37.0 9 38.17361713277164 39.0 39.0 39.0 37.0 39.0 10-11 38.14735072066475 39.0 39.0 39.0 37.0 39.0 12-13 38.110453351381246 39.0 39.0 39.0 37.0 39.0 14-15 39.63908684505196 41.0 40.0 41.0 37.0 41.0 16-17 39.65424926772815 41.0 40.0 41.0 37.0 41.0 18-19 39.665076094823235 41.0 40.0 41.0 37.0 41.0 20-21 39.658734859554016 41.0 40.0 41.0 37.0 41.0 22-23 39.58916113906679 41.0 40.0 41.0 37.0 41.0 24-25 39.540232278679795 41.0 40.0 41.0 37.0 41.0 26-27 39.43965606266472 41.0 39.0 41.0 36.0 41.0 28-29 39.348780315030524 41.0 39.0 41.0 36.0 41.0 30-31 39.26362170733706 41.0 39.0 41.0 36.0 41.0 32-33 39.306225761631 41.0 39.0 41.0 35.5 41.0 34-35 39.2519194398547 41.0 39.0 41.0 35.0 41.0 36-37 39.1704040553181 41.0 39.0 41.0 35.0 41.0 38-39 39.08250156183857 41.0 39.0 41.0 35.0 41.0 40-41 38.97184572619519 41.0 39.0 41.0 35.0 41.0 42-43 38.860180456364105 41.0 39.0 41.0 35.0 41.0 44-45 38.721412380003 41.0 39.0 41.0 35.0 41.0 46-47 38.51671316621376 41.0 38.5 41.0 35.0 41.0 48-49 38.33412314286299 41.0 38.0 41.0 34.5 41.0 50-51 38.19817405709331 40.0 38.0 41.0 34.0 41.0 52-53 38.055745261569555 40.0 38.0 41.0 34.0 41.0 54-55 37.90803544605427 40.0 37.0 41.0 34.0 41.0 56-57 37.73936861609155 40.0 37.0 41.0 34.0 41.0 58-59 37.54471317645533 40.0 36.5 41.0 33.0 41.0 60-61 37.34064253867898 40.0 36.0 41.0 33.0 41.0 62-63 37.097500546216764 39.0 35.5 41.0 33.0 41.0 64-65 36.8391875964414 39.0 35.0 41.0 33.0 41.0 66-67 36.549797473064686 38.5 35.0 41.0 32.0 41.0 68-69 36.22349205766683 37.5 35.0 40.5 32.0 41.0 70-71 35.893228917739755 37.0 35.0 40.0 32.0 41.0 72-73 35.544219874438426 36.5 35.0 39.0 31.0 41.0 74-75 35.21010868006718 36.0 35.0 39.0 31.0 41.0 76-77 34.87492169470579 36.0 35.0 39.0 31.0 40.5 78-79 34.54468501215332 35.0 35.0 37.0 31.0 39.5 80-81 34.21484668378146 35.0 35.0 37.0 31.0 39.0 82-83 33.90476241277601 35.0 34.0 36.5 30.5 39.0 84-85 33.64482617504882 35.0 34.0 36.0 30.0 37.0 86-87 33.42160359854434 35.0 34.0 36.0 30.0 37.0 88-89 33.21446753253404 35.0 34.0 35.5 30.0 37.0 90-91 33.026020827928065 35.0 34.0 35.0 30.0 36.0 92-93 32.86948854481025 35.0 34.0 35.0 30.0 36.0 94-95 32.72426252201936 35.0 34.0 35.0 29.0 36.0 96-97 32.60554081434092 35.0 34.0 35.0 29.0 36.0 98-99 32.499874114104685 35.0 34.0 35.0 29.0 35.5 100 32.40322703158498 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08889423159398291 1101 2 0.12516785626171156 1101 3 0.12283365087409948 1101 4 0.08426839321246149 1101 5 0.08322133750699123 1101 6 0.08260696813434265 1101 7 0.08159475743501332 1101 8 0.12316019383753485 1101 9 0.1276803741253687 1101 10-11 0.14876007518132184 1101 12-13 0.09372524786881087 1101 14-15 0.17053733599652077 1101 16-17 0.12507149040487064 1101 18-19 0.18131195695230673 1101 20-21 0.20205871906937745 1101 22-23 0.16364143789587615 1101 24-25 0.15028572498456327 1101 26-27 0.16965643911644435 1101 28-29 0.2268301443319629 1101 30-31 0.1969630022751403 1101 32-33 0.11293365823944868 1101 34-35 0.12801334560782607 1101 36-37 0.24554565246890547 1101 38-39 0.1753673826172104 1101 40-41 0.22190838745728314 1101 42-43 0.17111816111169276 1101 44-45 0.22845622068982152 1101 46-47 0.27628888877379865 1101 48-49 0.1234062470312054 1101 50-51 0.18399823150609507 1101 52-53 0.23736888680155488 1101 54-55 0.26421194503689804 1101 56-57 0.21929121033431187 1101 58-59 0.22729064512301278 1101 60-61 0.22413562907893692 1101 62-63 0.15497458834995825 1101 64-65 0.25851134447081847 1101 66-67 0.20773450593813436 1101 68-69 0.2461178363941201 1101 70-71 0.2418084034799648 1101 72-73 0.2660870041746861 1101 74-75 0.2660339650984085 1101 76-77 0.24193842703739676 1101 78-79 0.2592393523583709 1101 80-81 0.151185052510165 1101 82-83 0.183339560187477 1101 84-85 0.1984459098797018 1101 86-87 0.18356452436167103 1101 88-89 0.1824848444336311 1101 90-91 0.10933034116622053 1101 92-93 0.0953971416823407 1101 94-95 0.11858267821550328 1101 96-97 0.19850563848138592 1101 98-99 0.21853792397180172 1101 100 0.21595707706698164 1102 1 0.04175436312607417 1102 2 0.1100779375023464 1102 3 0.09409819521093965 1102 4 0.06481908727815267 1102 5 0.04772270371072551 1102 6 0.0846161409680306 1102 7 0.12836913198765387 1102 8 0.10790676202660165 1102 9 0.16342750800001937 1102 10-11 0.09553603019445944 1102 12-13 0.12547997948424694 1102 14-15 0.19542238234526366 1102 16-17 0.17493934303319492 1102 18-19 0.1465843691992248 1102 20-21 0.17939397122091805 1102 22-23 0.17261502422614683 1102 24-25 0.18468111783286645 1102 26-27 0.2466394771968936 1102 28-29 0.16347748465052803 1102 30-31 0.10770848362426477 1102 32-33 0.1271104386107993 1102 34-35 0.22123571471016135 1102 36-37 0.24600244531297477 1102 38-39 0.2366324428402109 1102 40-41 0.23704412963109434 1102 42-43 0.19352342517832 1102 44-45 0.18346438689939504 1102 46-47 0.243049667390153 1102 48-49 0.23197712407446147 1102 50-51 0.25841411467686726 1102 52-53 0.16350288460422036 1102 54-55 0.12507217875221244 1102 56-57 0.18063533334225212 1102 58-59 0.1834126383838921 1102 60-61 0.24020453874707215 1102 62-63 0.22253579565875725 1102 64-65 0.1657358282094208 1102 66-67 0.13911066259417737 1102 68-69 0.23020662487572707 1102 70-71 0.16570778917783002 1102 72-73 0.18256379193146444 1102 74-75 0.18198293868871218 1102 76-77 0.19212986652777886 1102 78-79 0.15658173348015936 1102 80-81 0.2094537981881217 1102 82-83 0.12520147585073005 1102 84-85 0.15576413281787183 1102 86-87 0.1968371123478505 1102 88-89 0.1941000859970785 1102 90-91 0.19645879254653664 1102 92-93 0.2510233971668612 1102 94-95 0.21423324098369534 1102 96-97 0.26443713257617674 1102 98-99 0.2872835323541665 1102 100 0.3550336249317283 1103 1 0.12444571148768802 1103 2 0.09449960598409746 1103 3 0.09615188588890788 1103 4 0.08350964669608629 1103 5 0.06962184190877707 1103 6 0.044959157659313576 1103 7 0.0392997331878604 1103 8 0.07757871656183113 1103 9 0.0969673156024271 1103 10-11 0.06505349270522487 1103 12-13 0.05070078594678762 1103 14-15 0.07460640752042735 1103 16-17 0.0638168807807773 1103 18-19 0.006534160944354994 1103 20-21 0.053815854130604635 1103 22-23 0.055534110173681484 1103 24-25 0.03443419980942508 1103 26-27 0.04776729259087631 1103 28-29 0.05335801617110292 1103 30-31 0.10849095668120867 1103 32-33 0.11978980262320249 1103 34-35 0.19026339357872502 1103 36-37 0.11138392348095039 1103 38-39 0.12581631971058727 1103 40-41 0.24966453635082786 1103 42-43 0.17206594241307727 1103 44-45 0.22492757136967612 1103 46-47 0.24419860440850982 1103 48-49 0.24628518258816 1103 50-51 0.18528738596967287 1103 52-53 0.2264298807701195 1103 54-55 0.15788796616514134 1103 56-57 0.19028709386648046 1103 58-59 0.2462651987715745 1103 60-61 0.2004129948215052 1103 62-63 0.11591145536709746 1103 64-65 0.18061245137933213 1103 66-67 0.1898543260832497 1103 68-69 0.2454969428307976 1103 70-71 0.26991258883081315 1103 72-73 0.2885396530131459 1103 74-75 0.25201453705441423 1103 76-77 0.21695589887314526 1103 78-79 0.23912609951828045 1103 80-81 0.20695746023004347 1103 82-83 0.17421847488542141 1103 84-85 0.2182001173401389 1103 86-87 0.21695999372659003 1103 88-89 0.21590334562293378 1103 90-91 0.14206083299368544 1103 92-93 0.13388901782192875 1103 94-95 0.07703346017250112 1103 96-97 0.14589187406440374 1103 98-99 0.12778886627278752 1103 100 0.08356010730182817 1104 1 0.04398276156055658 1104 2 0.11556999234846899 1104 3 0.10615844316537704 1104 4 0.042639700076442466 1104 5 0.04482133120449561 1104 6 0.05000238072047125 1104 7 0.10174450716999672 1104 8 0.11903071048699587 1104 9 0.11718795855150432 1104 10-11 0.14511845922769595 1104 12-13 0.14278911507203418 1104 14-15 0.15330427046183814 1104 16-17 0.21090694554825973 1104 18-19 0.18911692259310797 1104 20-21 0.168692499361498 1104 22-23 0.16336748084934527 1104 24-25 0.08488656241019754 1104 26-27 0.04591629085778948 1104 28-29 0.09565117116697763 1104 30-31 0.09667968755147882 1104 32-33 0.08289685970255078 1104 34-35 0.05004472822096062 1104 36-37 -0.007517065296447356 1104 38-39 0.024768251094194227 1104 40-41 0.060850946883810764 1104 42-43 -0.006439246892448125 1104 44-45 -0.017321247599277 1104 46-47 9.561964509643417E-4 1104 48-49 -0.05225546275667625 1104 50-51 -0.05590763064628845 1104 52-53 0.017415917295487304 1104 54-55 0.016868266762678275 1104 56-57 0.02678082994186326 1104 58-59 -0.027162273751685007 1104 60-61 -0.048660066312720573 1104 62-63 -0.0255254920645811 1104 64-65 -0.04015448519294296 1104 66-67 -0.11249875193811931 1104 68-69 -0.09510937972767408 1104 70-71 -0.07488579819968777 1104 72-73 0.013279941222883451 1104 74-75 -0.017585027181610258 1104 76-77 -0.12547837178004073 1104 78-79 -0.1283945926058081 1104 80-81 -0.042414430838448425 1104 82-83 -0.04591747675689817 1104 84-85 -0.02121465623478258 1104 86-87 -0.017855008357820168 1104 88-89 0.01957157665015785 1104 90-91 0.029117178277111577 1104 92-93 -0.0026055034466736515 1104 94-95 -0.03596397115003214 1104 96-97 3.335752019637539E-4 1104 98-99 0.010142406279932459 1104 100 -0.02326040976069521 1105 1 0.041979630809287016 1105 2 0.06914926190213322 1105 3 0.07698232015953721 1105 4 0.06250995672294835 1105 5 0.04135428598454638 1105 6 0.029329249805250868 1105 7 -0.007865284299548136 1105 8 0.0021774205095752563 1105 9 0.08700339443519312 1105 10-11 0.04379436899760236 1105 12-13 0.09179967919786947 1105 14-15 0.0477169161018125 1105 16-17 0.04745142335470831 1105 18-19 0.029890374005731246 1105 20-21 0.08126563558337807 1105 22-23 0.10663010694635489 1105 24-25 0.06964936101763186 1105 26-27 0.08111533721099562 1105 28-29 0.0459648192714468 1105 30-31 0.09651815185786461 1105 32-33 0.06482064094769413 1105 34-35 0.07816538185299038 1105 36-37 0.07780701365039278 1105 38-39 0.030256145305195048 1105 40-41 0.08422139331822365 1105 42-43 0.10072472228240059 1105 44-45 0.1557514001809821 1105 46-47 0.14525772845283313 1105 48-49 0.05066851400616201 1105 50-51 0.02483218431886769 1105 52-53 0.03740861491247216 1105 54-55 0.027662465264434388 1105 56-57 0.017091861741072023 1105 58-59 -0.021888849577557323 1105 60-61 -0.018778739733122052 1105 62-63 -0.032316602479895096 1105 64-65 -0.11377280186847827 1105 66-67 -0.0238646237169462 1105 68-69 -0.13646974562959713 1105 70-71 0.004598622178505707 1105 72-73 -0.027295444951001002 1105 74-75 -0.0673031132379549 1105 76-77 -0.09593633626986531 1105 78-79 -0.08955052528072827 1105 80-81 -0.01795488563482195 1105 82-83 -0.02095397544936617 1105 84-85 -0.08869439617685515 1105 86-87 -0.07703221032512175 1105 88-89 -0.03448117539797124 1105 90-91 0.030906015292408995 1105 92-93 0.09566179374250794 1105 94-95 0.026679791209765824 1105 96-97 -0.06805213095627494 1105 98-99 -0.09839375682378204 1105 100 -0.08242919336034049 1106 1 0.030558696150428943 1106 2 0.06146065610642637 1106 3 -0.0035401842035298614 1106 4 0.0012348134245314668 1106 5 0.007031534628268332 1106 6 0.021731857407878863 1106 7 -0.0504666103789404 1106 8 -0.042393998247298725 1106 9 -0.014709132949668913 1106 10-11 -0.030246314512581307 1106 12-13 -0.058458803231296486 1106 14-15 -0.017825371730324946 1106 16-17 0.07104038376542832 1106 18-19 0.04577367821858047 1106 20-21 -0.012247149133322921 1106 22-23 0.04741422701053466 1106 24-25 0.051118658787849824 1106 26-27 0.03088460233116308 1106 28-29 0.008511589066507952 1106 30-31 0.07358273461242959 1106 32-33 0.07610759029474679 1106 34-35 0.03182176347251442 1106 36-37 0.0074653085834981425 1106 38-39 -0.004456347395233706 1106 40-41 0.02432107939072381 1106 42-43 -0.0494278725636903 1106 44-45 -0.03740201708647106 1106 46-47 -0.028391946397242407 1106 48-49 0.023802499651473852 1106 50-51 0.04728335346786139 1106 52-53 0.08347554987215489 1106 54-55 0.04298260594985237 1106 56-57 0.04490757098944442 1106 58-59 0.008177512755416672 1106 60-61 -0.010606427785305073 1106 62-63 -0.020240748950939746 1106 64-65 -0.03408122321635432 1106 66-67 -0.04581632864042717 1106 68-69 -0.038857777431225315 1106 70-71 -0.0324047525504767 1106 72-73 0.03746602617604111 1106 74-75 0.017894369605755855 1106 76-77 -0.055325279871972555 1106 78-79 -0.03512547484655215 1106 80-81 -0.040134874182285785 1106 82-83 -0.056449160816001154 1106 84-85 -0.03369959550731494 1106 86-87 -0.012901979270083075 1106 88-89 -0.0685768937710236 1106 90-91 -0.10039571329320296 1106 92-93 -0.07242069551949726 1106 94-95 -0.08005410045405625 1106 96-97 0.028777644832189253 1106 98-99 0.028265968508826234 1106 100 0.06541488542269036 1107 1 0.005730349955221925 1107 2 0.011885363876608324 1107 3 0.05488469998770995 1107 4 0.01519348160115186 1107 5 -0.05993615048088685 1107 6 -0.07475100704755988 1107 7 -0.009649132240234337 1107 8 0.006652679393795324 1107 9 0.008778867174861205 1107 10-11 0.03430239132215718 1107 12-13 0.015626693530208513 1107 14-15 0.05922620418975555 1107 16-17 0.09879378473548428 1107 18-19 0.10910835416811437 1107 20-21 0.07911285621119646 1107 22-23 0.043372757682504925 1107 24-25 0.059256891178499416 1107 26-27 0.06318506167453819 1107 28-29 0.06595869842033153 1107 30-31 0.07205575191855473 1107 32-33 0.12072136436940895 1107 34-35 0.08177130185901405 1107 36-37 0.06789536087418213 1107 38-39 0.1274448295667696 1107 40-41 0.10493204759212205 1107 42-43 0.07440739167225985 1107 44-45 0.1383479021619749 1107 46-47 0.12719002907763155 1107 48-49 0.15805925456758985 1107 50-51 0.12291221024639043 1107 52-53 0.15437622481244517 1107 54-55 0.1404680544550061 1107 56-57 0.10879469782970119 1107 58-59 0.16657979299768044 1107 60-61 0.10451107383296687 1107 62-63 0.11138801313099123 1107 64-65 0.05721002980128986 1107 66-67 0.07105095212720869 1107 68-69 0.12125260896982581 1107 70-71 0.1353960032062318 1107 72-73 0.1332492059841357 1107 74-75 0.10424064394311472 1107 76-77 0.10163709314701919 1107 78-79 0.16805208257785154 1107 80-81 0.08884319382543993 1107 82-83 0.09178215314474159 1107 84-85 0.11576390933063863 1107 86-87 0.05965426467355428 1107 88-89 0.0842330981473367 1107 90-91 0.041643999607451576 1107 92-93 -0.006161451250264349 1107 94-95 -0.013869932428562493 1107 96-97 0.08021022279797307 1107 98-99 0.07866126195802536 1107 100 0.08281473728088429 1108 1 -0.03131283789836914 1108 2 -0.034075468282736665 1108 3 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-0.05374202898838831 2214 10-11 -0.12478975205988974 2214 12-13 -0.10286805859816184 2214 14-15 -0.12245437345232801 2214 16-17 -0.1672237785074131 2214 18-19 -0.15950521262521988 2214 20-21 -0.1958935222454059 2214 22-23 -0.16920189571696653 2214 24-25 -0.1968733571881316 2214 26-27 -0.21938997075518074 2214 28-29 -0.21557484943281224 2214 30-31 -0.14417583612195983 2214 32-33 -0.2114644124926599 2214 34-35 -0.2265694423640312 2214 36-37 -0.2563760383527125 2214 38-39 -0.2795069244097945 2214 40-41 -0.1406753031053185 2214 42-43 -0.18238219959172142 2214 44-45 -0.11869018019393707 2214 46-47 -0.12220472469132204 2214 48-49 -0.2010225361975344 2214 50-51 -0.1664695970942347 2214 52-53 -0.2816288332612089 2214 54-55 -0.2166816721057998 2214 56-57 -0.18183444427777573 2214 58-59 -0.21119709849048718 2214 60-61 -0.28024492403767454 2214 62-63 -0.32408032115557717 2214 64-65 -0.1929342986900764 2214 66-67 -0.3076464312630094 2214 68-69 -0.1961696573028746 2214 70-71 -0.20633709275234935 2214 72-73 -0.261307552096703 2214 74-75 -0.2231530168610334 2214 76-77 -0.22435910580198737 2214 78-79 -0.37692190576498774 2214 80-81 -0.31766284604967154 2214 82-83 -0.2716658396638678 2214 84-85 -0.281678428573116 2214 86-87 -0.2735811996582882 2214 88-89 -0.3650436753888826 2214 90-91 -0.27905129176213705 2214 92-93 -0.17913699135419847 2214 94-95 -0.20506600759874516 2214 96-97 -0.3610101074787906 2214 98-99 -0.37350604142286414 2214 100 -0.5150354541293822 2215 1 -8.477009914287237E-4 2215 2 -0.025521452540445466 2215 3 -0.004194635542134506 2215 4 -0.0039016629206116704 2215 5 0.015510744931880538 2215 6 -0.03712872539797729 2215 7 -0.05406616129890551 2215 8 -0.03862785223486043 2215 9 -0.04695193343648896 2215 10-11 -0.01991645897178529 2215 12-13 -0.019948873615327045 2215 14-15 -0.05553365673051047 2215 16-17 -0.06686778792267489 2215 18-19 -0.06858212940974084 2215 20-21 -0.026762654954417542 2215 22-23 -0.030699469546718205 2215 24-25 -0.02078741668150741 2215 26-27 0.00841195272054307 2215 28-29 -0.008815740261965743 2215 30-31 -0.018311392629470902 2215 32-33 -0.03679767454895 2215 34-35 -0.062494162798948594 2215 36-37 -0.03994806608187673 2215 38-39 -0.024960501166205518 2215 40-41 -0.08638999782548495 2215 42-43 -0.029553146588618517 2215 44-45 -0.056766331348164556 2215 46-47 -0.16352161359646544 2215 48-49 -0.10846097782295061 2215 50-51 -0.09418522272135021 2215 52-53 -0.10942360511365479 2215 54-55 -0.1348599862313975 2215 56-57 -0.21105564207183392 2215 58-59 -0.20028895953659998 2215 60-61 -0.16372877631813765 2215 62-63 -0.1438166008620172 2215 64-65 -0.14982107270762413 2215 66-67 -0.14699227060884823 2215 68-69 -0.12113059245774593 2215 70-71 -0.03331122181498358 2215 72-73 -0.02675117159304108 2215 74-75 -0.09704143575193314 2215 76-77 -0.09161654677897246 2215 78-79 -0.07586051159071161 2215 80-81 -0.054670810183438334 2215 82-83 -0.04382756011002442 2215 84-85 -0.08880740104272178 2215 86-87 -0.09037191948232248 2215 88-89 -0.06512103968803018 2215 90-91 -0.08645993097088223 2215 92-93 -0.03628923649749538 2215 94-95 -0.00954852068394274 2215 96-97 0.007734285134326058 2215 98-99 -0.045976346041349814 2215 100 -0.01552482607574035 2216 1 -0.07087473653570697 2216 2 -0.09012479300585596 2216 3 -0.12590434345874257 2216 4 -0.07351404287781804 2216 5 -0.026643142049593393 2216 6 -0.026463783698552845 2216 7 -0.009863875717307735 2216 8 -0.016327674762266042 2216 9 -0.05805641005820661 2216 10-11 -0.09906591743158089 2216 12-13 -0.09355024032014114 2216 14-15 -0.17392197339469107 2216 16-17 -0.13928323964847067 2216 18-19 -0.0968326145567815 2216 20-21 -0.11929304920894879 2216 22-23 -0.07750164565923967 2216 24-25 -0.17852654855856542 2216 26-27 -0.22702119650571007 2216 28-29 -0.3082509793523798 2216 30-31 -0.19982687586909265 2216 32-33 -0.1792152824837956 2216 34-35 -0.18642642466224402 2216 36-37 -0.21006052067708936 2216 38-39 -0.19457536905693473 2216 40-41 -0.21797680182608303 2216 42-43 -0.14364563137076658 2216 44-45 -0.09980758128596534 2216 46-47 -0.19495250709632472 2216 48-49 -0.27045447602334605 2216 50-51 -0.22883633151039362 2216 52-53 -0.15606406554721985 2216 54-55 -0.14936049211121372 2216 56-57 -0.1303387854929028 2216 58-59 -0.16597620970660643 2216 60-61 -0.2312595852282584 2216 62-63 -0.17429793306749275 2216 64-65 -0.15158335895267783 2216 66-67 -0.14183810166157684 2216 68-69 -0.09681518883199658 2216 70-71 -0.026975284444574754 2216 72-73 -0.03933182210472097 2216 74-75 -0.061663021063182555 2216 76-77 -0.024040125754282826 2216 78-79 0.007800399656403556 2216 80-81 -0.03872315630475498 2216 82-83 -0.09497118021916151 2216 84-85 -0.06775948344307636 2216 86-87 -0.06960182112602098 2216 88-89 -0.09524576808861696 2216 90-91 -0.05588322798038092 2216 92-93 0.0010753784533221733 2216 94-95 -0.0067786213623222125 2216 96-97 -0.08413680673676538 2216 98-99 -0.012181915695194334 2216 100 -0.0028114857761920575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 12.0 4 8.0 5 19.0 6 41.0 7 126.0 8 239.0 9 452.0 10 906.0 11 1406.0 12 1976.0 13 3266.0 14 3900.0 15 5157.0 16 6327.0 17 9241.0 18 11158.0 19 6309.0 20 4405.0 21 4174.0 22 4363.0 23 4734.0 24 5330.0 25 6591.0 26 8086.0 27 10558.0 28 13579.0 29 17622.0 30 22250.0 31 28695.0 32 36977.0 33 48640.0 34 68431.0 35 106635.0 36 205527.0 37 474405.0 38 825008.0 39 395252.0 40 1574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60301595321881 19.25734736040026 13.12840011224615 24.01123657413478 2 15.923062676615904 19.6086648157647 40.11458750291248 24.35368500470691 3 18.686331608198714 24.709523630702847 29.60418060657372 26.999964154524726 4 12.399418959931449 16.229947801982092 38.13310153180187 33.237531706284585 5 12.785128676902191 37.651649203093285 35.48916860014643 14.0740535198581 6 31.806243009589956 37.58565052579832 17.16437177096931 13.443734693642412 7 28.047447677353745 32.110270273038374 21.82565846326778 18.016623586340103 8 25.791303924802605 36.03109014802983 19.681707483083187 18.495898444084375 9 25.443119107889945 16.016383117093714 20.693798892885003 37.84669888213134 10-11 24.40273834937318 26.25698109463564 28.46413250931079 20.876148046680388 12-13 25.2860383580724 23.521779540085483 28.83821827504745 22.35396382679466 14-15 22.239848629581797 25.491547396816706 26.118118171085868 26.15048580251563 16-17 21.609316751102675 29.049898608512787 26.61421990004233 22.726564740342205 18-19 21.40858359227094 27.583037305578095 29.285805880755206 21.72257322139576 20-21 22.483050210976227 26.90512300118802 29.0303329532575 21.581493834578254 22-23 22.024386502183912 26.670995160209383 29.24212459191004 22.062493745696663 24-25 21.957766730626727 26.76309252047088 29.136086851972326 22.143053896930066 26-27 21.734452096079885 26.81181421995103 29.191215614314903 22.262518069654185 28-29 21.731658264650182 26.83050332079699 29.28626070256862 22.15157771198421 30-31 21.944289462911083 27.273365653919857 28.8815972864026 21.90074759676646 32-33 21.671362981260202 26.894761789819803 29.39922814399641 22.03464708492358 34-35 21.837073518668273 26.740943210948416 29.333408459313414 22.0885748110699 36-37 22.18743470671457 27.11296274598562 28.759500673234463 21.940101874065352 38-39 21.951540161669385 27.252085746749838 28.848846212618547 21.947527878962237 40-41 21.791318731158125 27.03992743376998 29.019107328655657 22.149646506416243 42-43 21.87800515013106 26.95726108296167 28.91325234660476 22.25148142030251 44-45 21.950020754437126 27.152139094210202 28.759823677985036 22.138016473367628 46-47 22.11669599847547 27.39593076370043 28.44400142636457 22.04337181145953 48-49 22.10676451835103 27.39652978185664 28.20224416999767 22.29446152979466 50-51 22.11860115615077 27.324736424211864 28.127631943663523 22.42903047597384 52-53 22.11260558173576 27.41372890632418 28.128521391603815 22.34514412033624 54-55 22.287178156598095 27.69103630865558 27.9663320670269 22.055453467719428 56-57 21.99434226119226 27.448482699259475 28.05208721401385 22.50508782553442 58-59 21.859124769589457 27.096514573126797 28.44482831680764 22.599532340476106 60-61 22.19488755994541 27.45053943403167 28.047539837721718 22.3070331683012 62-63 21.98580758260347 27.42002522868833 28.14462456536842 22.44954262333978 64-65 22.036847175650863 27.155252520187638 28.278185614260064 22.529714689901432 66-67 21.902304936430593 27.23841402826298 28.142171351808187 22.71710968349824 68-69 22.248075264143765 27.399708155784815 28.03170301277197 22.320513567299454 70-71 22.269691550831713 27.20099923849458 28.05484972696542 22.474459483708287 72-73 22.029933659983733 27.340975564376613 28.072567767440393 22.55652300819926 74-75 22.1377573685201 27.39676134610633 27.939033388083285 22.526447897290282 76-77 22.12459281667485 27.363764946057746 27.854315903209265 22.65732633405814 78-79 22.307409843732763 27.521758546754604 27.80628231030309 22.364549299209543 80-81 22.154715182405564 27.375386034769477 27.944415260540662 22.525483522284294 82-83 22.062222969481248 27.41743866442432 28.03927576375823 22.4810626023362 84-85 22.06104285400637 27.315191070700457 27.87410442110168 22.74966165419149 86-87 22.167690540362926 27.467495779614815 27.810567434842206 22.554246245180057 88-89 21.94057202989769 27.441936185225558 27.812382181978556 22.805109602898195 90-91 21.920306939814996 27.24809901548653 27.94650999342406 22.885084051274415 92-93 22.009451261014487 27.447425546995785 27.781065985147162 22.762057206842563 94-95 21.803010337152298 27.239467404514485 27.984541212055003 22.972981046278214 96-97 21.8026902882659 27.230228659993717 27.909543089675136 23.057537962065243 98-99 21.800385936283813 27.317751362128064 27.82957354125985 23.052289160328275 100 21.948141838838744 27.204027324493723 27.70253546995125 23.145295366716283 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 49.0 1 42.0 2 112.5 3 209.0 4 142.0 5 96.0 6 249.5 7 587.5 8 1028.0 9 1241.5 10 1016.5 11 770.5 12 800.5 13 928.0 14 1104.0 15 1231.0 16 1391.5 17 1600.5 18 1840.5 19 2215.0 20 2614.5 21 3251.5 22 4340.0 23 5997.5 24 7944.0 25 10197.5 26 13430.5 27 17715.5 28 23108.0 29 28649.0 30 33800.5 31 39885.5 32 46195.5 33 52195.5 34 58299.0 35 63906.0 36 68515.0 37 72847.0 38 76605.5 39 78575.5 40 79873.0 41 81083.0 42 81358.5 43 81299.0 44 80716.0 45 79821.5 46 79758.0 47 78928.5 48 77168.5 49 75598.5 50 73596.5 51 71321.0 52 67491.5 53 64264.0 54 62136.5 55 59460.0 56 56451.0 57 52167.0 58 47683.0 59 43798.5 60 39715.0 61 34704.0 62 30067.5 63 25761.0 64 21537.0 65 17603.5 66 14023.5 67 10685.0 68 7950.5 69 6066.0 70 4432.5 71 3224.0 72 2503.5 73 1917.5 74 1439.0 75 1068.5 76 711.5 77 498.0 78 361.5 79 205.5 80 121.5 81 60.0 82 28.5 83 14.0 84 6.0 85 4.0 86 2.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08999774685583974 2 0.0012801955456022722 3 0.0 4 3.413854788272726E-4 5 0.03827784681350794 6 1.2801955456022722E-4 7 0.004053952561073862 8 5.547514030943179E-4 9 1.706927394136363E-4 10-11 4.053952561073862E-4 12-13 0.0 14-15 6.400977728011361E-5 16-17 0.0 18-19 1.066829621335227E-4 20-21 0.0 22-23 2.3470251669374992E-4 24-25 1.066829621335227E-4 26-27 0.0012588589531755676 28-29 0.008726666302522156 30-31 0.034949338394942034 32-33 0.025497227949911924 34-35 0.028185638595676694 36-37 0.025497227949911924 38-39 0.025006486324097715 40-41 0.030063258729226695 42-43 0.023000846635987494 44-45 0.022168719531346016 46-47 0.01589576135789488 48-49 0.010838988952765905 50-51 0.007915875790307385 52-53 0.00531281151424943 54-55 0.004011279376220453 56-57 0.0022616787972306812 58-59 8.748002894948861E-4 60-61 4.267318485340908E-4 62-63 0.001194849175895454 64-65 0.0027524204230448854 66-67 3.627220712539771E-4 68-69 7.894539197880678E-4 70-71 3.2004888640056804E-4 72-73 4.267318485340908E-4 74-75 4.6940503338749985E-4 76-77 2.7737570154715897E-4 78-79 1.066829621335227E-4 80-81 6.400977728011361E-4 82-83 5.547514030943179E-4 84-85 2.3470251669374992E-4 86-87 1.706927394136363E-4 88-89 2.1336592426704536E-5 90-91 4.267318485340907E-5 92-93 4.267318485340907E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2343392.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.66720224239845 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2321004829326 54.29081769711035 2 12.706012050179707 17.195605741877245 3 3.356649685324885 6.8140527934128645 4 1.319899657346776 3.5725566821342687 5 0.6752747711956963 2.284697725584426 6 0.4054403003569558 1.646100648088374 7 0.26865972603842003 1.272561641136039 8 0.18695091967105978 1.0120356552627192 9 0.1284973758600335 0.7825552127944538 >10 0.6760961245512984 8.055938219729292 >50 0.03441683628536093 1.5689150988639193 >100 0.00968633471950214 1.0414837327293596 >500 6.314710755987932E-5 0.022988675968729413 >1k 2.525884302369041E-4 0.4396904753081381 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4010 0.1711194712621704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3388 0.14457675028334993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.267318485340908E-5 0.0 5 0.0 0.0 0.0 4.267318485340908E-5 0.0 6 0.0 0.0 0.0 4.267318485340908E-5 0.0 7 0.0 0.0 0.0 4.267318485340908E-5 0.0 8 0.0 0.0 0.0 4.267318485340908E-5 0.0 9 0.0 0.0 0.0 1.2801955456022722E-4 0.0 10-11 0.0 0.0 0.0 1.2801955456022722E-4 0.0 12-13 0.0 0.0 0.0 1.4935614698693175E-4 0.0 14-15 0.0 0.0 0.0 2.5603910912045443E-4 0.0 16-17 0.0 0.0 0.0 2.7737570154715897E-4 0.0 18-19 0.0 0.0 0.0 2.987122939738635E-4 0.0 20-21 0.0 0.0 0.0 3.840586636806817E-4 0.0 22-23 0.0 0.0 0.0 4.694050333874998E-4 0.0 24-25 0.0 0.0 0.0 5.334148106676134E-4 0.0 26-27 0.0 0.0 0.0 7.467807349346589E-4 0.0 28-29 0.0 0.0 0.0 0.0015362346547227266 0.0 30-31 0.0 0.0 0.0 0.00484340648086193 0.0 32-33 0.0 0.0 0.0 0.01109502806188636 0.0 34-35 0.0 0.0 0.0 0.020504465322063063 0.0 36-37 0.0 0.0 0.0 0.037616412448280095 0.0 38-39 0.0 0.0 0.0 0.06714625636683919 0.0 40-41 0.0 0.0 0.0 0.10297039505127609 0.0 42-43 0.0 0.0 0.0 0.13902923625240676 0.0 44-45 0.0 0.0 0.0 0.18080628422389425 0.0 46-47 0.0 0.0 0.0 0.23148069123731752 0.0 48-49 0.0 0.0 0.0 0.2897295885622209 0.0 50-51 0.0 0.0 0.0 0.35484886864852316 0.0 52-53 0.0 0.0 0.0 0.4232753205609646 0.0 54-55 0.0 0.0 0.0 0.493728748753943 0.0 56-57 0.0 2.133659242670454E-5 0.0 0.5754052245633681 0.0 58-59 0.0 4.267318485340908E-5 0.0 0.6712918709289781 0.0 60-61 0.0 4.267318485340908E-5 0.0 0.7737288511695866 0.0 62-63 0.0 4.267318485340908E-5 0.0 0.8814573063320179 0.0 64-65 0.0 4.267318485340908E-5 0.0 0.9904659570400514 0.0 66-67 0.0 4.267318485340908E-5 0.0 1.114687598148325 0.0 68-69 0.0 4.267318485340908E-5 0.0 1.257813460146659 0.0 70-71 0.0 4.267318485340908E-5 0.0 1.4150001365541915 0.0 72-73 0.0 4.267318485340908E-5 0.0 1.579974669197471 0.0 74-75 0.0 6.400977728011362E-5 0.0 1.7476802856713687 0.0 76-77 0.0 8.534636970681816E-5 0.0 1.9241979148174952 0.0 78-79 0.0 8.534636970681816E-5 0.0 2.1325070666794117 0.0 80-81 0.0 8.534636970681816E-5 0.0 2.359677766246535 0.0 82-83 0.0 8.534636970681816E-5 0.0 2.6017627439199247 0.0 84-85 0.0 8.534636970681816E-5 0.0 2.838428227116931 0.0 86-87 0.0 8.534636970681816E-5 0.0 3.082305478554164 0.0 88 0.0 8.534636970681816E-5 0.0 3.2860485996367657 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3085 0.0 51.098755 1 GTATCAA 5840 0.0 42.302612 1 TCAACGC 6605 0.0 36.943237 4 ATCAACG 6625 0.0 36.760746 3 CAACGCA 6750 0.0 36.150417 5 AACGCAG 6995 0.0 35.00597 6 TATCAAC 7260 0.0 33.805206 2 ACGCAGA 8210 0.0 29.710936 7 CGCAGAG 8405 0.0 28.852026 8 TAGCGCG 125 8.0081245E-6 26.328642 4 GTACATG 7255 0.0 25.42549 1 GCAGAGT 9730 0.0 25.116253 9 TACATGG 7155 0.0 24.904583 2 ACATGGG 7345 0.0 23.299683 3 GAGTACT 6100 0.0 20.338982 12-13 AGAGTAC 8850 0.0 20.232338 10-11 TATACCG 140 5.2004715E-4 20.149902 5 CAGAGTA 9700 0.0 19.98557 10-11 GTATAAC 620 0.0 18.974442 1 CTAGTAC 460 0.0 18.397343 3 >>END_MODULE