FastQCFastQC Report
Wed 25 May 2016
SRR1033192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4122200
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.033.826851
TCAACGC45350.032.2111324
ATCAACG46000.031.865433
GTATCAA49150.031.7375831
CAACGCA46700.031.2799725
AACGCAG52100.028.2990916
TATCAAC60350.024.8466842
ACGCAGA59550.024.4436347
GTACATG93300.024.34641
CGCAGAG59950.023.9672438
TACATGG91750.023.0548062
ACATGGG96500.021.2266863
TATACCG3052.4374458E-1020.0201075
CTGTCGC10700.019.3087779
GCAGAGT77400.017.775129
GAGTACA72150.017.505231
GTGTACG4103.6379788E-1117.2415681
CATGGGG58500.016.5399514
AGAGTAC64600.015.95467110-11
TCTGTCG11650.015.71898658
Produced by FastQC (version 0.11.5)