##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033192_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4122200
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.793840910193587	33.0	31.0	34.0	30.0	34.0
2	31.880752995973022	34.0	31.0	34.0	30.0	34.0
3	31.963509048566298	34.0	31.0	34.0	30.0	34.0
4	35.32865896851196	37.0	35.0	37.0	33.0	37.0
5	35.400267090388624	37.0	35.0	37.0	33.0	37.0
6	35.49271481247877	37.0	37.0	37.0	35.0	37.0
7	35.396947746349035	37.0	36.0	37.0	33.0	37.0
8	35.41973533550046	37.0	36.0	37.0	35.0	37.0
9	37.095697443112904	39.0	38.0	39.0	35.0	39.0
10-11	37.08645395662511	39.0	38.0	39.0	34.5	39.0
12-13	37.06202270632187	39.0	38.0	39.0	35.0	39.0
14-15	38.54669460482266	41.0	39.0	41.0	35.0	41.0
16-17	38.57471059143176	41.0	39.0	41.0	35.0	41.0
18-19	38.57653461743729	41.0	39.0	41.0	35.0	41.0
20-21	38.59519334336034	41.0	39.0	41.0	35.0	41.0
22-23	38.57154128863229	41.0	39.0	41.0	34.0	41.0
24-25	38.576441584590754	41.0	39.0	41.0	34.0	41.0
26-27	38.53665748871961	41.0	39.0	41.0	34.0	41.0
28-29	38.51036351947989	40.5	39.0	41.0	34.0	41.0
30-31	38.51530978603658	41.0	39.0	41.0	34.0	41.0
32-33	38.57738295085149	41.0	39.0	41.0	34.0	41.0
34-35	38.55084214739702	41.0	39.0	41.0	34.0	41.0
36-37	38.4824805928873	41.0	39.0	41.0	34.0	41.0
38-39	38.41105368492552	41.0	39.0	41.0	34.0	41.0
40-41	38.35603524816845	41.0	39.0	41.0	34.0	41.0
42-43	38.26271711707341	41.0	39.0	41.0	33.5	41.0
44-45	38.167139027703655	40.5	38.0	41.0	33.0	41.0
46-47	38.06268351850953	40.0	38.0	41.0	33.0	41.0
48-49	37.96255045849304	40.0	38.0	41.0	33.0	41.0
50-51	37.86322861578769	40.0	38.0	41.0	33.0	41.0
52-53	37.736504900295955	40.0	38.0	41.0	33.0	41.0
54-55	37.60983188588618	40.0	38.0	41.0	32.5	41.0
56-57	37.47672395808064	40.0	37.0	41.0	32.0	41.0
58-59	37.303058682257046	40.0	37.0	41.0	32.0	41.0
60-61	37.109747222356994	40.0	36.5	41.0	31.0	41.0
62-63	36.86861700063073	39.5	36.0	41.0	31.0	41.0
64-65	36.6165814370967	39.0	35.5	41.0	31.0	41.0
66-67	36.34790973266702	39.0	35.0	41.0	31.0	41.0
68-69	36.055014191451164	38.5	35.0	40.5	30.5	41.0
70-71	35.71278237348989	37.5	35.0	40.0	30.0	41.0
72-73	35.3480463102227	37.0	35.0	40.0	30.0	41.0
74-75	34.97375940032022	36.5	35.0	39.0	30.0	41.0
76-77	34.625969870457524	36.0	34.0	39.0	29.0	40.5
78-79	34.234361627286404	36.0	34.0	38.5	29.0	40.0
80-81	33.85185580515259	35.0	34.0	37.0	29.0	39.0
82-83	33.59510916500898	35.0	34.0	37.0	29.0	39.0
84-85	33.29507884139537	35.0	34.0	36.5	29.0	38.5
86-87	33.01418065596041	35.0	34.0	36.0	29.0	37.0
88-89	32.6235952889234	35.0	34.0	36.0	28.0	37.0
90-91	32.41819441075154	35.0	34.0	35.5	27.5	36.5
92-93	32.23362791713163	35.0	34.0	35.0	27.0	36.0
94-95	32.06239216923002	35.0	34.0	35.0	27.0	36.0
96-97	31.89015295230702	35.0	34.0	35.0	27.0	36.0
98-99	31.701974795012372	35.0	33.5	35.0	26.0	36.0
100	31.54349449323177	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2442908187318764
1101	2	0.18507480530412934
1101	3	0.14885601946416216
1101	4	0.07861853955740372
1101	5	0.1433537501590436
1101	6	0.09229724189808053
1101	7	0.15550402367733085
1101	8	0.06903766299200953
1101	9	0.13227193388848946
1101	10-11	0.10979089385693186
1101	12-13	0.14396977138216727
1101	14-15	0.17746736632436466
1101	16-17	0.13125540127448687
1101	18-19	0.1649056452377522
1101	20-21	0.12483710806425563
1101	22-23	0.11892990926245517
1101	24-25	0.11764916869310582
1101	26-27	0.1583878271103316
1101	28-29	0.2130960256443828
1101	30-31	0.18937049738971723
1101	32-33	0.13224551802804285
1101	34-35	0.21762313999552418
1101	36-37	0.20599241021012205
1101	38-39	0.19231738140685906
1101	40-41	0.2149199078394659
1101	42-43	0.17151769155677954
1101	44-45	0.2321468366366375
1101	46-47	0.26747630226539343
1101	48-49	0.15931463722635186
1101	50-51	0.15096463240919178
1101	52-53	0.20430398233858682
1101	54-55	0.16250660844679032
1101	56-57	0.1725830224870819
1101	58-59	0.21942546870090496
1101	60-61	0.12923747530679464
1101	62-63	0.1479273838127
1101	64-65	0.07690043728032236
1101	66-67	0.14079529469182006
1101	68-69	0.17510648741661328
1101	70-71	0.18238209349146217
1101	72-73	0.1648266926124009
1101	74-75	0.15358345556695951
1101	76-77	0.16624232395489713
1101	78-79	0.2096846503681391
1101	80-81	0.19603873430823882
1101	82-83	0.1606305755886055
1101	84-85	0.09410595948715184
1101	86-87	0.15575482616693392
1101	88-89	0.24890136567574217
1101	90-91	0.3185302771885645
1101	92-93	0.26109824241634527
1101	94-95	0.29857004787048425
1101	96-97	0.2761034504659392
1101	98-99	0.3362305020376084
1101	100	0.37284597549277265
1102	1	0.17167803085131084
1102	2	0.1586729676284726
1102	3	0.11731981231890387
1102	4	0.08353141196185021
1102	5	-0.042565036417038016
1102	6	0.013836406504154297
1102	7	0.03580392220366946
1102	8	0.007698788232104903
1102	9	0.07034550441368026
1102	10-11	0.06284554713278112
1102	12-13	0.07897328883556298
1102	14-15	0.13762442549332832
1102	16-17	0.08176768198677564
1102	18-19	0.10592280176042834
1102	20-21	0.1101969895458268
1102	22-23	0.10310490019383423
1102	24-25	0.1324713785488285
1102	26-27	0.16075607698103056
1102	28-29	0.17514624722894467
1102	30-31	0.18999242664916238
1102	32-33	0.13814928355314748
1102	34-35	0.16103420229894994
1102	36-37	0.15808407624554377
1102	38-39	0.06750512891920124
1102	40-41	0.12458432353595583
1102	42-43	0.09658740313309266
1102	44-45	0.11694792552221145
1102	46-47	0.15301641260893462
1102	48-49	0.12487559354367761
1102	50-51	0.08527362781499903
1102	52-53	0.07994381761077562
1102	54-55	0.09099864967724614
1102	56-57	0.026632300084152405
1102	58-59	0.03983031708665408
1102	60-61	-0.0017073834238203744
1102	62-63	0.09730155460493961
1102	64-65	0.13321169115621245
1102	66-67	0.06161050014034686
1102	68-69	0.053840375442590016
1102	70-71	0.041225526315976424
1102	72-73	0.07671176638516641
1102	74-75	0.06404886065034532
1102	76-77	-0.005533462515934673
1102	78-79	0.1338894046551431
1102	80-81	0.11999277316722612
1102	82-83	0.05956491470860925
1102	84-85	0.018172340607605975
1102	86-87	0.06018993615172974
1102	88-89	0.26710806193701586
1102	90-91	0.22432583789738914
1102	92-93	0.22621414213067226
1102	94-95	0.24138633680465205
1102	96-97	0.2683811465364627
1102	98-99	0.32083673725499295
1102	100	0.3217997768549061
1103	1	0.20779957831021534
1103	2	0.21530875442943298
1103	3	0.21410667810629747
1103	4	0.22815556038222695
1103	5	0.20490012926666878
1103	6	0.24016526669159077
1103	7	0.24606955601094427
1103	8	0.2308556271328115
1103	9	0.22660878794509642
1103	10-11	0.27584392893380993
1103	12-13	0.29668767514834116
1103	14-15	0.3495514023936721
1103	16-17	0.28772916489155875
1103	18-19	0.2902441028677316
1103	20-21	0.27455247516668635
1103	22-23	0.30669559907493493
1103	24-25	0.27300474255437734
1103	26-27	0.2910357167084072
1103	28-29	0.31412059773708023
1103	30-31	0.30145964321052077
1103	32-33	0.3187653659687797
1103	34-35	0.34831682924771457
1103	36-37	0.36157526747975055
1103	38-39	0.3445167165952512
1103	40-41	0.4017131956268969
1103	42-43	0.3237874607672495
1103	44-45	0.27756434415718445
1103	46-47	0.26425610766703755
1103	48-49	0.3156172354747824
1103	50-51	0.3180291367116581
1103	52-53	0.2533253741747572
1103	54-55	0.288257861393177
1103	56-57	0.2783096996128194
1103	58-59	0.20104530575721924
1103	60-61	0.2476381206474514
1103	62-63	0.2898745658131716
1103	64-65	0.2509714308632809
1103	66-67	0.2374790989797262
1103	68-69	0.24354687526880525
1103	70-71	0.25154258900718673
1103	72-73	0.23596448961097138
1103	74-75	0.3099347159325987
1103	76-77	0.2213180165413462
1103	78-79	0.21881690260492803
1103	80-81	0.2285150674073222
1103	82-83	0.2431890888434296
1103	84-85	0.22085242530349092
1103	86-87	0.2535335204527769
1103	88-89	0.289134684714476
1103	90-91	0.31194176264606455
1103	92-93	0.3468922899133773
1103	94-95	0.3123466522979186
1103	96-97	0.35051846153584876
1103	98-99	0.4217456552528418
1103	100	0.43823621878258123
1104	1	0.13678219530865832
1104	2	0.1413469454377072
1104	3	0.09779153937837748
1104	4	0.061203841479219534
1104	5	0.03421939940850649
1104	6	0.024014009955735105
1104	7	0.06258489381455234
1104	8	0.05129746155571979
1104	9	0.07668136450276819
1104	10-11	0.08758428171879018
1104	12-13	0.12936712526713023
1104	14-15	0.04138942589631256
1104	16-17	0.12275688259214235
1104	18-19	0.1076243910101482
1104	20-21	0.09660966800590387
1104	22-23	0.08233180766819004
1104	24-25	0.07941227669007844
1104	26-27	0.043951654757826475
1104	28-29	0.133424023514209
1104	30-31	0.11801064134846229
1104	32-33	0.14310747024650539
1104	34-35	0.08209653318282761
1104	36-37	0.1109473085793482
1104	38-39	0.07088038160655685
1104	40-41	0.05071795832667192
1104	42-43	0.1158549173304877
1104	44-45	0.06803221292854289
1104	46-47	0.0430824471389073
1104	48-49	0.012080646301072306
1104	50-51	0.00946488687085889
1104	52-53	-0.015298048201110248
1104	54-55	0.005919540682683078
1104	56-57	0.005492342512532389
1104	58-59	0.013678088653989562
1104	60-61	0.07177450109010408
1104	62-63	0.10707877025299695
1104	64-65	-0.012600638902853234
1104	66-67	0.018623720243347464
1104	68-69	-0.018609078837549475
1104	70-71	0.0220764317014428
1104	72-73	0.08760106563817516
1104	74-75	0.04341345298297483
1104	76-77	-0.047340936547279
1104	78-79	-0.036801112304658545
1104	80-81	0.04541371059785604
1104	82-83	0.1036652510909235
1104	84-85	0.07778363922619747
1104	86-87	0.0737132168525676
1104	88-89	0.20175017321324162
1104	90-91	0.11538907136236531
1104	92-93	0.09405641478745963
1104	94-95	0.1057596815816737
1104	96-97	0.17150986261367507
1104	98-99	0.16878658202518082
1104	100	0.2166952683326997
1105	1	0.1979682672850558
1105	2	0.17972786722691225
1105	3	0.14982810183691697
1105	4	0.1488633300583544
1105	5	0.09651583543667641
1105	6	0.08834039131445337
1105	7	0.05551146663770368
1105	8	0.0580915727773359
1105	9	0.07343356835553294
1105	10-11	0.03019994096928258
1105	12-13	0.04916175654845745
1105	14-15	0.12461493994574369
1105	16-17	0.14925956086638337
1105	18-19	0.06696122510345504
1105	20-21	0.11698306522278301
1105	22-23	0.15995362849564998
1105	24-25	0.1619213785662481
1105	26-27	0.16121015535652816
1105	28-29	0.21637092821126913
1105	30-31	0.2653606977270542
1105	32-33	0.2403509459744555
1105	34-35	0.22239161718876232
1105	36-37	0.20351506846871814
1105	38-39	0.1663534430790179
1105	40-41	0.2091568165628459
1105	42-43	0.25737038761043607
1105	44-45	0.24066364688012243
1105	46-47	0.2707616108669342
1105	48-49	0.2553611024639295
1105	50-51	0.22315751196533995
1105	52-53	0.2509607174152322
1105	54-55	0.204871515629371
1105	56-57	0.19292818712906268
1105	58-59	0.21963050285826569
1105	60-61	0.20595900823211366
1105	62-63	0.23515477877479896
1105	64-65	0.22693266071305374
1105	66-67	0.25849764400405917
1105	68-69	0.2111115436912101
1105	70-71	0.20866888345376822
1105	72-73	0.23853347383715828
1105	74-75	0.1583721275223553
1105	76-77	0.18554185848767446
1105	78-79	0.14389390725909124
1105	80-81	0.11991625386937699
1105	82-83	0.17857093207818764
1105	84-85	0.15989467430130588
1105	86-87	0.24542631647159396
1105	88-89	0.31496919150411173
1105	90-91	0.28637902529850834
1105	92-93	0.2754869663666426
1105	94-95	0.35329754468826025
1105	96-97	0.39981890044668944
1105	98-99	0.3779165477391473
1105	100	0.44246120089718133
1106	1	0.09460566071929932
1106	2	0.12215279946607538
1106	3	0.026567978854714625
1106	4	0.10598550808893492
1106	5	0.03204593998272287
1106	6	0.07171906974950559
1106	7	0.054994923790410155
1106	8	0.0665720096237905
1106	9	0.06242058891255908
1106	10-11	-0.024442654006421094
1106	12-13	0.0209318559560856
1106	14-15	0.06175964464553374
1106	16-17	0.07734836398073242
1106	18-19	0.0750241650683563
1106	20-21	0.07633945654167462
1106	22-23	0.008681156565160109
1106	24-25	0.027987364942546833
1106	26-27	0.08298307708437846
1106	28-29	0.11052751276113071
1106	30-31	0.14443254992262666
1106	32-33	0.13739410751108494
1106	34-35	0.08471101318470176
1106	36-37	0.10438709393250178
1106	38-39	0.0951932190069158
1106	40-41	0.031341177509325746
1106	42-43	0.1312101320321304
1106	44-45	0.08839354035050917
1106	46-47	0.07088517077539791
1106	48-49	0.09323745793881955
1106	50-51	0.10362132931705759
1106	52-53	0.03661048727963845
1106	54-55	0.0549416182511564
1106	56-57	0.07666381758748031
1106	58-59	0.01988211297077669
1106	60-61	0.039271703679773395
1106	62-63	0.10535729110576852
1106	64-65	0.10513009668014206
1106	66-67	0.12869682038429886
1106	68-69	0.0756466993432312
1106	70-71	0.10378509659263813
1106	72-73	0.11496607272652426
1106	74-75	0.13342927685926753
1106	76-77	0.12375680766658803
1106	78-79	0.07281356067393574
1106	80-81	0.06569077187612749
1106	82-83	0.007990030269034776
1106	84-85	0.05302988869782865
1106	86-87	0.040287440577913
1106	88-89	0.054669112675071574
1106	90-91	0.08929689403266394
1106	92-93	0.12541053867636975
1106	94-95	0.12889319590053105
1106	96-97	0.11105259803936463
1106	98-99	0.12235625919317528
1106	100	0.15646826495818544
1107	1	0.11487410710978097
1107	2	0.08901205312368177
1107	3	0.0819981926695732
1107	4	0.08259037890289989
1107	5	0.03142160581831632
1107	6	-0.020952549764665207
1107	7	-0.06305755864516271
1107	8	-0.12378679018307537
1107	9	-0.014141402814175308
1107	10-11	-0.06450688206557231
1107	12-13	-0.0992026816959779
1107	14-15	-0.11648908224959342
1107	16-17	-0.0816901425793759
1107	18-19	-0.042728336758720786
1107	20-21	-0.023592528178987493
1107	22-23	-0.01370982245237684
1107	24-25	-0.0550160177070822
1107	26-27	0.021466737312863415
1107	28-29	0.015588496775883698
1107	30-31	0.015119753061775043
1107	32-33	0.02466108445199211
1107	34-35	-0.008284565542282962
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2216	3	0.024549833808674038
2216	4	0.08481564250418927
2216	5	0.0658509932405309
2216	6	0.10387787565766615
2216	7	0.10186048561207883
2216	8	0.09990397511688087
2216	9	0.04121747362629691
2216	10-11	0.02280511808076824
2216	12-13	0.0013391574873011791
2216	14-15	-0.071836580700392
2216	16-17	-0.03605631340489168
2216	18-19	-0.0859685669254091
2216	20-21	-0.06602120516438248
2216	22-23	-0.07604691141769848
2216	24-25	-0.05644731636293443
2216	26-27	-0.12204175502291292
2216	28-29	-0.09451769715482072
2216	30-31	-0.1596636606050268
2216	32-33	-0.12112131095189227
2216	34-35	-0.106067354155158
2216	36-37	-0.11599303532324257
2216	38-39	-0.1604827265632025
2216	40-41	-0.15425888217667705
2216	42-43	-0.1662704094725953
2216	44-45	-0.1348673228280859
2216	46-47	-0.09613606976193267
2216	48-49	-0.07093179267700123
2216	50-51	-0.08186990534329652
2216	52-53	-0.03934554164613502
2216	54-55	-0.017793856629637617
2216	56-57	-0.07994945986570912
2216	58-59	-0.12677150243530377
2216	60-61	-0.06629552870067101
2216	62-63	-0.020468902682182488
2216	64-65	-0.03825361704827657
2216	66-67	-0.07113759567200617
2216	68-69	-0.07795957704765044
2216	70-71	-0.12274463593136176
2216	72-73	-0.01626596119729129
2216	74-75	-0.021724218258597716
2216	76-77	-0.021040125910879226
2216	78-79	-0.05689242808766437
2216	80-81	-0.04420549206611213
2216	82-83	-0.06607580904354649
2216	84-85	-0.04911991527303172
2216	86-87	-0.09181326868166195
2216	88-89	-0.029161643505297263
2216	90-91	-0.051287758309449316
2216	92-93	-0.06090790597399831
2216	94-95	-0.04998421933219532
2216	96-97	-0.08483556665410674
2216	98-99	-0.06027455483221189
2216	100	-0.006665975786599887
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36857.0
3	6194.0
4	3805.0
5	2974.0
6	2774.0
7	3178.0
8	3867.0
9	4282.0
10	4744.0
11	5246.0
12	5914.0
13	6662.0
14	7685.0
15	8083.0
16	8006.0
17	7895.0
18	8078.0
19	7906.0
20	7942.0
21	8460.0
22	9586.0
23	12888.0
24	19554.0
25	28179.0
26	29513.0
27	25392.0
28	25977.0
29	32357.0
30	39945.0
31	48988.0
32	64298.0
33	88119.0
34	122289.0
35	183695.0
36	336711.0
37	751489.0
38	1420975.0
39	729846.0
40	1847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.807801514249796	20.713165161788133	13.749563384412793	23.72946993954928
2	14.688010896866205	20.539514377310308	41.64386163525373	23.128613090569754
3	17.429164230039014	26.212457424255774	30.764357047091305	25.594021298613907
4	11.610828651514968	17.15909283954812	40.91249954496366	30.317578963973258
5	12.058487397731634	38.682349252222295	36.927159415485214	12.332003934560865
6	29.741449270861665	39.69425367613967	18.31277871736576	12.251518335632905
7	25.89232579077061	33.20245508318136	23.846471660985223	17.058747465062808
8	23.823571942941772	37.76689027218926	21.215034948068006	17.194502836800964
9	24.260399679821347	17.217536842018426	22.056005060356203	36.46605841780403
10-11	22.801352131239664	27.567429132063786	30.25555610418976	19.37566263250679
12-13	23.538205603227244	25.012825923736433	29.993299827212454	21.45566864582387
14-15	20.851903910260468	27.184109724783017	26.908271601253837	25.05571476370268
16-17	20.50577569046725	30.504072390724346	27.500429464465093	21.48972245434332
18-19	20.417996152059526	29.557540224856666	29.404170959780785	20.620292663303026
20-21	21.702349448779998	28.88934398583721	28.734341416211016	20.673965149171774
22-23	21.20401489535981	28.716888686591847	28.85499200301246	21.22410441503589
24-25	21.18041877068506	28.838319787662837	28.759968470206903	21.2212929714452
26-27	20.897083592095235	28.695110883140472	28.888407389888144	21.51939813487615
28-29	20.900213956526404	28.781398933080478	28.963800632697932	21.35458647769519
30-31	21.183252041436788	29.292549404784147	28.50735994557139	21.016838608207674
32-33	20.784970922801442	28.953738906860394	29.08430816431538	21.176982006022783
34-35	20.970496915131108	28.65601667714407	29.115914352034384	21.257572055690442
36-37	21.30267848792192	29.03485128831942	28.537263411029855	21.12520681272881
38-39	21.086818886689255	29.16893467375332	28.719150503919128	21.025095935638298
40-41	20.887297457880628	28.86043393209321	29.01838688319682	21.233881726829342
42-43	20.858635532366353	28.72333534376136	29.014734189228992	21.403294934643295
44-45	21.023131371492713	28.900852571062853	28.915845924130966	21.16017013331347
46-47	21.09876454402568	29.125789377725496	28.733913276805968	21.04153280144285
48-49	21.020438562527232	28.897004666653082	28.82910093420398	21.25345583661571
50-51	21.006937431623097	28.804867252826327	28.820102891508302	21.368092424042274
52-53	21.0871246329802	28.81631147903901	28.81488009422879	21.281683793752006
54-55	21.20876528287148	29.174071443690437	28.59107375382318	21.026089519614903
56-57	21.058687547086414	28.892823530282257	28.66986545976657	21.378623462864763
58-59	20.874652571486518	28.536165437228036	29.11713553696673	21.472046454318715
60-61	21.18324793660802	28.948477032766945	28.663957406480367	21.20431762414467
62-63	20.99262396306511	28.944185613786487	28.717830591927907	21.345359831220495
64-65	20.975797856113648	28.668078489728583	28.84100033150631	21.515123322651462
66-67	20.865079763626312	28.710732858868393	28.755879563826063	21.668307813679235
68-69	21.370861850146884	28.835994848687786	28.621975462975826	21.171167838189504
70-71	21.274152498890732	28.676806832652087	28.683793581361293	21.365247087095888
72-73	21.04647542207516	28.785303425787344	28.835083835231213	21.333137316906285
74-75	21.072652074802317	28.839971165123334	28.682405521565933	21.404971238508413
76-77	21.119113386585518	28.86321324825535	28.602309740219727	21.41536362493941
78-79	21.30085198167799	28.914296499521956	28.634034580475625	21.150816938324425
80-81	21.229574374555437	28.66145014056188	28.721178022080394	21.38779746280229
82-83	21.074707820338695	28.737007313179504	28.87742191227072	21.310862954211085
84-85	21.091575439031036	28.679491725072936	28.719993314023412	21.508939521872616
86-87	21.19588969397106	28.837424817946776	28.614527504638133	21.352157983444027
88-89	21.05422249492376	28.64729257283285	28.731751135602185	21.56673379664121
90-91	21.075806789797852	28.553161803670797	28.774821367679298	21.596210038852057
92-93	21.198725211703994	28.72975894033528	28.598382130898898	21.473133717061827
94-95	20.975449218268572	28.450604233256954	28.84132961822884	21.732616930245634
96-97	21.018746722185032	28.5616075466191	28.756391205340265	21.6632545258556
98-99	21.06437874024539	28.57130480990448	28.688698716427396	21.675617733422733
100	21.170815110450132	28.53771230813046	28.54913351364846	21.742339067770942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	270.0
2	249.5
3	213.5
4	169.5
5	142.0
6	131.0
7	139.0
8	155.0
9	171.0
10	172.5
11	189.5
12	216.0
13	241.0
14	284.5
15	370.5
16	544.5
17	767.0
18	1103.5
19	1725.0
20	2750.5
21	4356.5
22	6944.0
23	11185.5
24	16466.5
25	23041.5
26	32087.5
27	43703.5
28	57743.5
29	72280.5
30	85837.5
31	99186.5
32	112548.5
33	125824.5
34	137840.0
35	146486.5
36	151903.5
37	157233.0
38	162457.5
39	163614.5
40	163098.5
41	161576.0
42	156000.0
43	150089.5
44	144605.5
45	138668.5
46	134002.5
47	128374.0
48	122100.5
49	117540.5
50	112999.0
51	106944.5
52	99458.5
53	92748.0
54	87511.5
55	82489.5
56	75894.0
57	68385.0
58	61626.5
59	54773.5
60	47221.0
61	39353.5
62	32470.5
63	26734.5
64	21896.5
65	17571.0
66	13885.5
67	10609.0
68	8048.0
69	6266.5
70	4611.0
71	3357.0
72	2589.0
73	2073.0
74	1624.5
75	1182.5
76	818.5
77	614.5
78	458.0
79	316.0
80	228.0
81	145.5
82	93.0
83	55.0
84	33.5
85	28.0
86	24.5
87	17.0
88	17.0
89	15.5
90	12.0
91	10.5
92	7.0
93	3.5
94	5.0
95	5.5
96	4.0
97	5.0
98	8.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33707728882635485
2	0.12258017563437
3	0.06736693998350396
4	0.04002716995778953
5	0.04344767357236427
6	0.010115957498423173
7	0.0073504439377031685
8	0.015913832419581775
9	0.018218427053515115
10-11	0.020729222259958272
12-13	0.016120032992091603
14-15	0.0184610159623502
16-17	0.019116006016204938
18-19	0.01878851098927757
20-21	0.02372519528407161
22-23	0.015719761292513706
24-25	0.02470768036485372
26-27	0.019686089951967394
28-29	0.01945563048857406
30-31	0.02032895056038038
32-33	0.024634903692203194
34-35	0.015307360147494056
36-37	0.010079569162097908
38-39	0.01350007277667265
40-41	0.00977633302605405
42-43	0.008684682936296153
44-45	0.009060695744990539
46-47	0.008769589054388433
48-49	0.004560671486099655
50-51	0.0069259133472417645
52-53	0.007702197855514046
54-55	0.0034568919509000047
56-57	0.0030202319149968465
58-59	0.0031293969239726362
60-61	0.003990587550337199
62-63	0.003287079714715443
64-65	0.002583571879093688
66-67	0.002013487943331231
68-69	0.0034811508417835137
70-71	0.002692736888069477
72-73	0.0023409829702585997
74-75	0.0026320896608607054
76-77	0.004318082577264568
78-79	0.003954199214011935
80-81	0.004172529231963515
82-83	0.00294745524234632
84-85	0.003384115278249478
86-87	0.002971714133229829
88-89	0.6046407258260152
90-91	0.6156178739508029
92-93	0.6114696036097229
94-95	0.599024792586483
96-97	0.596210761243996
98-99	0.5933118237834166
100	0.5957741012080927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4122200.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.894272859548714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.86085677049493	43.75914620103467
2	15.931768047790722	19.40306861274416
3	5.799886265732182	10.595395704595337
4	2.54945495790364	6.20988823398854
5	1.351070025519089	4.113621339315843
6	0.7916089632359534	2.8922671323212765
7	0.46514358337999206	1.9827206199646636
8	0.297630318117935	1.4499185442198252
9	0.19724435727988687	1.080994654098697
>10	0.7266855031005837	6.903718242140385
>50	0.021601136541757944	0.8946655544174035
>100	0.007010192722496153	0.6995393736345729
>500	3.9878180862182415E-5	0.015055787524697849
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.8517781767017614E-5	0.0
5	0.0	0.0	0.0	4.8517781767017614E-5	0.0
6	0.0	0.0	0.0	7.277667265052642E-5	0.0
7	0.0	0.0	0.0	7.277667265052642E-5	0.0
8	0.0	0.0	0.0	7.277667265052642E-5	0.0
9	0.0	0.0	0.0	1.2129445441754403E-4	0.0
10-11	0.0	0.0	0.0	1.3342389985929843E-4	0.0
12-13	0.0	1.2129445441754403E-5	0.0	1.4555334530105284E-4	0.0
14-15	0.0	4.8517781767017614E-5	0.0	1.6981223618456163E-4	0.0
16-17	0.0	7.277667265052642E-5	0.0	1.6981223618456163E-4	0.0
18-19	0.0	7.277667265052642E-5	0.0	1.6981223618456163E-4	0.0
20-21	0.0	7.277667265052642E-5	0.0	1.6981223618456163E-4	0.0
22-23	0.0	7.277667265052642E-5	0.0	1.6981223618456163E-4	0.0
24-25	0.0	7.277667265052642E-5	0.0	1.9407112706807045E-4	0.0
26-27	0.0	7.277667265052642E-5	0.0	2.911066906021057E-4	0.0
28-29	0.0	7.277667265052642E-5	0.0	8.975789626898259E-4	0.0
30-31	0.0	8.490611809228082E-5	0.0	0.003796516423269128	0.0
32-33	0.0	9.703556353403523E-5	0.0	0.010758818106836154	0.0
34-35	0.0	9.703556353403523E-5	0.0	0.02102032895056038	0.0
36-37	0.0	1.3342389985929843E-4	0.0	0.03875357818640532	0.0
38-39	0.0	1.6981223618456163E-4	0.0	0.06952598127213624	0.0
40-41	0.0	1.6981223618456163E-4	0.0	0.10589005870651594	0.0
42-43	0.0	1.9407112706807045E-4	0.0	0.14353985735772162	0.0
44-45	0.0	1.9407112706807045E-4	0.0	0.1874848381931978	0.0
46-47	0.0	1.9407112706807045E-4	0.0	0.23962932414729998	0.0
48-49	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.30076172917374216	0.0
50-51	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.36754645577604195	0.0
52-53	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.4345252535054097	0.0
54-55	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.5061132405026443	0.0
56-57	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.5928266459657465	0.0
58-59	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.6930279947600796	0.0
60-61	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.7979234389403717	0.0
62-63	2.4258890883508807E-5	1.9407112706807045E-4	0.0	0.9063485517442142	0.0
64-65	2.4258890883508807E-5	1.9407112706807045E-4	0.0	1.0249745281645724	0.0
66-67	2.4258890883508807E-5	1.9407112706807045E-4	0.0	1.1592596186502353	0.0
68-69	2.4258890883508807E-5	1.9407112706807045E-4	0.0	1.30945854155548	0.0
70-71	2.4258890883508807E-5	1.9407112706807045E-4	0.0	1.472538935519868	0.0
72-73	2.4258890883508807E-5	1.9407112706807045E-4	0.0	1.6429940323128427	0.0
74-75	2.4258890883508807E-5	2.0620057250982484E-4	0.0	1.8155475231672407	0.0
76-77	2.4258890883508807E-5	2.911066906021057E-4	0.0	2.001297850662268	0.0
78-79	2.4258890883508807E-5	2.911066906021057E-4	0.0	2.216268012226481	0.0
80-81	2.4258890883508807E-5	2.911066906021057E-4	0.0	2.451263888215031	0.0
82-83	2.4258890883508807E-5	2.911066906021057E-4	0.0	2.6947382465673666	0.0
84-85	2.4258890883508807E-5	2.911066906021057E-4	0.0	2.939255737227694	0.0
86-87	2.4258890883508807E-5	2.911066906021057E-4	0.0	3.196460627820096	0.0
88	2.4258890883508807E-5	2.911066906021057E-4	0.0	3.4086652758235894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3065	0.0	33.82685	1
TCAACGC	4535	0.0	32.211132	4
ATCAACG	4600	0.0	31.86543	3
GTATCAA	4915	0.0	31.737583	1
CAACGCA	4670	0.0	31.279972	5
AACGCAG	5210	0.0	28.299091	6
TATCAAC	6035	0.0	24.846684	2
ACGCAGA	5955	0.0	24.443634	7
GTACATG	9330	0.0	24.3464	1
CGCAGAG	5995	0.0	23.967243	8
TACATGG	9175	0.0	23.054806	2
ACATGGG	9650	0.0	21.226686	3
TATACCG	305	2.4374458E-10	20.020107	5
CTGTCGC	1070	0.0	19.308777	9
GCAGAGT	7740	0.0	17.77512	9
GAGTACA	7215	0.0	17.50523	1
GTGTACG	410	3.6379788E-11	17.241568	1
CATGGGG	5850	0.0	16.539951	4
AGAGTAC	6460	0.0	15.954671	10-11
TCTGTCG	1165	0.0	15.7189865	8
>>END_MODULE