FastQCFastQC Report
Wed 25 May 2016
SRR1033192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4122200
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58430.14174469943234194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49500.1200815098733686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.052.779541
GTATCAA96450.040.6862221
TCAACGC110350.034.5912744
ATCAACG111000.034.4310653
CAACGCA112600.033.9000635
AACGCAG118200.032.284966
TATCAAC121100.031.676282
ACGCAGA133600.028.3876087
CGCAGAG135650.027.8882968
GTACATG90450.025.8542181
TACATGG87200.025.3917482
GCAGAGT156950.024.1026449
ACATGGG91850.023.3894463
GAGTACT108650.021.69058612-13
AGAGTAC138700.021.53124610-11
CAGAGTA153050.020.41824710-11
GGTACCG2401.5837395E-719.5817036
GTATACG3456.002665E-1119.0938051
AGTACTT121700.018.88201712-13
GTACTTT125700.018.59892714-15
Produced by FastQC (version 0.11.5)