##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033192_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4122200 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76779122798506 34.0 31.0 34.0 31.0 34.0 2 32.867158070932994 34.0 31.0 34.0 31.0 34.0 3 32.96362476347581 34.0 31.0 34.0 31.0 34.0 4 36.37418004948814 37.0 37.0 37.0 35.0 37.0 5 36.31481150841783 37.0 37.0 37.0 35.0 37.0 6 36.39660254233176 37.0 37.0 37.0 35.0 37.0 7 36.36615448061715 37.0 37.0 37.0 35.0 37.0 8 36.3665596040949 37.0 37.0 37.0 35.0 37.0 9 38.1906341274077 39.0 39.0 39.0 37.0 39.0 10-11 38.16730350298384 39.0 39.0 39.0 37.0 39.0 12-13 38.13376109844258 39.0 39.0 39.0 37.0 39.0 14-15 39.66799488137403 41.0 40.0 41.0 37.0 41.0 16-17 39.69050288680802 41.0 40.0 41.0 37.0 41.0 18-19 39.7075329920916 41.0 40.0 41.0 37.0 41.0 20-21 39.70566724079375 41.0 40.0 41.0 37.0 41.0 22-23 39.63462617049149 41.0 40.0 41.0 37.0 41.0 24-25 39.59244238513415 41.0 40.0 41.0 37.0 41.0 26-27 39.4933347484353 41.0 39.5 41.0 36.5 41.0 28-29 39.406758163116784 41.0 39.0 41.0 36.0 41.0 30-31 39.319216195235555 41.0 39.0 41.0 36.0 41.0 32-33 39.354358594925046 41.0 39.0 41.0 36.0 41.0 34-35 39.30236342244433 41.0 39.0 41.0 36.0 41.0 36-37 39.21990696715346 41.0 39.0 41.0 35.0 41.0 38-39 39.13356435883752 41.0 39.0 41.0 35.0 41.0 40-41 39.02868249963612 41.0 39.0 41.0 35.0 41.0 42-43 38.92690141186745 41.0 39.0 41.0 35.0 41.0 44-45 38.80078380476445 41.0 39.0 41.0 35.0 41.0 46-47 38.61108582795595 41.0 39.0 41.0 35.0 41.0 48-49 38.448327106884676 41.0 39.0 41.0 35.0 41.0 50-51 38.34076670224637 41.0 39.0 41.0 35.0 41.0 52-53 38.22452598127214 41.0 38.0 41.0 34.0 41.0 54-55 38.10648015622726 40.0 38.0 41.0 34.0 41.0 56-57 37.97039165979331 40.0 38.0 41.0 34.0 41.0 58-59 37.80534241424482 40.0 37.5 41.0 34.0 41.0 60-61 37.63787989423123 40.0 37.0 41.0 33.5 41.0 62-63 37.426460870409 40.0 36.5 41.0 33.0 41.0 64-65 37.19439340643346 39.5 36.0 41.0 33.0 41.0 66-67 36.93446399980593 39.0 35.5 41.0 33.0 41.0 68-69 36.62876219009267 39.0 35.0 41.0 32.5 41.0 70-71 36.3046019116006 38.0 35.0 40.5 32.0 41.0 72-73 35.95771444859541 37.0 35.0 40.0 32.0 41.0 74-75 35.61506283052739 37.0 35.0 39.0 31.5 41.0 76-77 35.26351183833876 36.0 35.0 39.0 31.0 41.0 78-79 34.89969858328077 36.0 35.0 39.0 31.0 40.0 80-81 34.53754439377032 35.5 35.0 37.0 31.0 39.0 82-83 34.188296661976615 35.0 35.0 37.0 31.0 39.0 84-85 33.8884034010965 35.0 34.0 36.5 31.0 38.5 86-87 33.62796031245452 35.0 34.0 36.0 30.5 37.0 88-89 33.38657549366843 35.0 34.0 36.0 30.0 37.0 90-91 33.16795861433215 35.0 34.0 35.5 30.0 36.5 92-93 32.990431808257725 35.0 34.0 35.0 30.0 36.0 94-95 32.83132174566978 35.0 34.0 35.0 30.0 36.0 96-97 32.70131313376352 35.0 34.0 35.0 30.0 36.0 98-99 32.589047353355 35.0 34.0 35.0 30.0 36.0 100 32.4919028674009 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10304393518793376 1101 2 0.15312107076238135 1101 3 0.11269038080698834 1101 4 0.0682447048962942 1101 5 0.03199282097833134 1101 6 0.0913739909593616 1101 7 0.09533774406864381 1101 8 0.07705465517658894 1101 9 0.15775518148856804 1101 10-11 0.15034660752105822 1101 12-13 0.15706143102024583 1101 14-15 0.22338658131027955 1101 16-17 0.20509036367577949 1101 18-19 0.18865779566515073 1101 20-21 0.19248617473508034 1101 22-23 0.1963945009568846 1101 24-25 0.13711623391254335 1101 26-27 0.21353939993930027 1101 28-29 0.23208017184035157 1101 30-31 0.18885427825107115 1101 32-33 0.16257390268639682 1101 34-35 0.14775939457067722 1101 36-37 0.16173415121790669 1101 38-39 0.18903264787599028 1101 40-41 0.19452893382255354 1101 42-43 0.1201484451218704 1101 44-45 0.16181929225299996 1101 46-47 0.1702519490031591 1101 48-49 0.16377606440788384 1101 50-51 0.16151799412149614 1101 52-53 0.19657609130472764 1101 54-55 0.18591864612899656 1101 56-57 0.21078971441428962 1101 58-59 0.16897495211754432 1101 60-61 0.19092400048332792 1101 62-63 0.17317535409368645 1101 64-65 0.23290716582812365 1101 66-67 0.23969488945355266 1101 68-69 0.23951725445250815 1101 70-71 0.2446683104433518 1101 72-73 0.2562798328505451 1101 74-75 0.2419545918365955 1101 76-77 0.25755792591532156 1101 78-79 0.20792279726117613 1101 80-81 0.19703742311670425 1101 82-83 0.19501768967072763 1101 84-85 0.24325923921219328 1101 86-87 0.11884649943038994 1101 88-89 0.18073701363103822 1101 90-91 0.17451685362755143 1101 92-93 0.1045586088759336 1101 94-95 0.18009214181816446 1101 96-97 0.18848706731206022 1101 98-99 0.17668112330636632 1101 100 0.133160179795361 1102 1 0.15474456265462067 1102 2 0.14304932921295688 1102 3 0.1666407909640526 1102 4 0.10577077017592984 1102 5 0.0730897139444977 1102 6 0.09102918302806984 1102 7 0.06933715107650329 1102 8 0.0976383824422058 1102 9 0.17091813207947126 1102 10-11 0.11488057744595181 1102 12-13 0.15857247349894266 1102 14-15 0.17440751153294798 1102 16-17 0.14983837701460345 1102 18-19 0.15316382846021526 1102 20-21 0.19421996426237342 1102 22-23 0.1824662480062358 1102 24-25 0.1686218477131547 1102 26-27 0.184714719366319 1102 28-29 0.20306105634362837 1102 30-31 0.1556949051127674 1102 32-33 0.14442720563013722 1102 34-35 0.1476388649176883 1102 36-37 0.1141237820552945 1102 38-39 0.15861698838347849 1102 40-41 0.13338324400294255 1102 42-43 0.09859257448715653 1102 44-45 0.1032228811554532 1102 46-47 0.08096438206342782 1102 48-49 0.06682044281987487 1102 50-51 0.04279050476072399 1102 52-53 0.07926780188955718 1102 54-55 0.05503813029582716 1102 56-57 0.020997416662233093 1102 58-59 0.062061837466309555 1102 60-61 0.05798838688021135 1102 62-63 0.08457905924459652 1102 64-65 0.003942199613923947 1102 66-67 -0.002228873806586762 1102 68-69 0.02498450326145729 1102 70-71 0.07148444887952365 1102 72-73 0.10289580268894838 1102 74-75 0.09246766628836411 1102 76-77 0.0581777552025855 1102 78-79 0.1226192712829004 1102 80-81 0.08442104028377173 1102 82-83 0.08785332834851545 1102 84-85 0.130208330886731 1102 86-87 0.0655340495703598 1102 88-89 0.10128262890162887 1102 90-91 0.09237572324752819 1102 92-93 0.11353464619344322 1102 94-95 0.09639887575393402 1102 96-97 0.10202074424084628 1102 98-99 0.1316462512684211 1102 100 0.1551204513217641 1103 1 0.08698806441594087 1103 2 0.08979532352462627 1103 3 0.101899552496171 1103 4 0.06287271272697126 1103 5 0.04156353646148858 1103 6 0.07653224383137314 1103 7 0.0778491444491749 1103 8 0.087961844769886 1103 9 0.13983921083531925 1103 10-11 0.1051613030954286 1103 12-13 0.11644756964137315 1103 14-15 0.14214260219688413 1103 16-17 0.14418927327155018 1103 18-19 0.13598446853734458 1103 20-21 0.11451080788077661 1103 22-23 0.06127622588577708 1103 24-25 0.08913272787987125 1103 26-27 0.1422005374893942 1103 28-29 0.17361038443009136 1103 30-31 0.2122256007318839 1103 32-33 0.18026622622137012 1103 34-35 0.21999778045879026 1103 36-37 0.2464835903583591 1103 38-39 0.2097051861427488 1103 40-41 0.24348539190734186 1103 42-43 0.2618030279993633 1103 44-45 0.32213140450301836 1103 46-47 0.3344131354139961 1103 48-49 0.3534822802821509 1103 50-51 0.3045749626864023 1103 52-53 0.3317061919578421 1103 54-55 0.3079695766800725 1103 56-57 0.3459376909471956 1103 58-59 0.41148031381197825 1103 60-61 0.38697467166608845 1103 62-63 0.2921884545773423 1103 64-65 0.31535875993186124 1103 66-67 0.3705929137624224 1103 68-69 0.392209566681359 1103 70-71 0.38389881707145435 1103 72-73 0.3412889917179811 1103 74-75 0.3495697869088943 1103 76-77 0.29805903174474935 1103 78-79 0.26945864946181075 1103 80-81 0.3203348983518808 1103 82-83 0.34625967218585885 1103 84-85 0.34119977896353504 1103 86-87 0.3063500249511293 1103 88-89 0.3047814409064884 1103 90-91 0.2903415388547046 1103 92-93 0.34514252739224105 1103 94-95 0.3748303261824688 1103 96-97 0.33791991912113417 1103 98-99 0.35980169700171416 1103 100 0.34891489852343227 1104 1 0.04678195920539707 1104 2 0.05446653236157317 1104 3 0.06643025155048576 1104 4 0.036484238147025394 1104 5 0.010947123098411282 1104 6 0.024670855032454142 1104 7 0.01968281842799513 1104 8 0.07729802217330928 1104 9 0.09512665329342695 1104 10-11 0.07300770752556218 1104 12-13 0.07220105925569698 1104 14-15 0.11696281995881463 1104 16-17 0.11350501021742332 1104 18-19 0.10022762374067895 1104 20-21 0.08611726043103829 1104 22-23 0.06282252480971096 1104 24-25 0.08633019430241262 1104 26-27 0.02162609155823958 1104 28-29 0.08030890747006225 1104 30-31 0.041383362396643975 1104 32-33 0.09245432359677608 1104 34-35 0.10663735103231886 1104 36-37 0.14130349184365087 1104 38-39 0.15081508207563843 1104 40-41 0.11273552305556223 1104 42-43 0.11964533754006368 1104 44-45 0.13845883423631022 1104 46-47 0.20106086868990758 1104 48-49 0.15608942518443314 1104 50-51 0.1952381020332794 1104 52-53 0.2481119160003189 1104 54-55 0.27647774243575896 1104 56-57 0.312236889102401 1104 58-59 0.3126012062628334 1104 60-61 0.2863897154418851 1104 62-63 0.2887659986368618 1104 64-65 0.31081064146673043 1104 66-67 0.3174680026972254 1104 68-69 0.2991987156852218 1104 70-71 0.28134362737518614 1104 72-73 0.3120353907443345 1104 74-75 0.29561987775345244 1104 76-77 0.22132576954773242 1104 78-79 0.2053638648692413 1104 80-81 0.3003136081529192 1104 82-83 0.27487060653155027 1104 84-85 0.24622912183056656 1104 86-87 0.262169707980874 1104 88-89 0.246687501331337 1104 90-91 0.2402560951957753 1104 92-93 0.24897589523371266 1104 94-95 0.19908067279055075 1104 96-97 0.21956190123198382 1104 98-99 0.19050750028041108 1104 100 0.22102371819401867 1105 1 0.1436561310252742 1105 2 0.12960934095665522 1105 3 0.12355701477401482 1105 4 0.07792125888324364 1105 5 0.07137754566022636 1105 6 0.04489058690908365 1105 7 0.026057540068038065 1105 8 0.03472810922345815 1105 9 0.0827786435692417 1105 10-11 0.02769305810200251 1105 12-13 0.03310143922514186 1105 14-15 0.04723878245998492 1105 16-17 0.10285446839434798 1105 18-19 0.08687985409520849 1105 20-21 0.08267477441263793 1105 22-23 0.14213911182145011 1105 24-25 0.08708684127398669 1105 26-27 0.09448100321293396 1105 28-29 0.1297940101203423 1105 30-31 0.12957762464839107 1105 32-33 0.09092614942864685 1105 34-35 0.14970047278048781 1105 36-37 0.08604008193452017 1105 38-39 0.1532049792175343 1105 40-41 0.20958853835443136 1105 42-43 0.1981164534438733 1105 44-45 0.25957243536048935 1105 46-47 0.29915401357807525 1105 48-49 0.2177557377183632 1105 50-51 0.21855258819001477 1105 52-53 0.2153832352062537 1105 54-55 0.23168099134584708 1105 56-57 0.2521884477353069 1105 58-59 0.23597045829793473 1105 60-61 0.2291721745328985 1105 62-63 0.24544804264441922 1105 64-65 0.1886164367407659 1105 66-67 0.1912143645237805 1105 68-69 0.20396195052309452 1105 70-71 0.2718229019650451 1105 72-73 0.20778594763051927 1105 74-75 0.2116116971211497 1105 76-77 0.1434385154330542 1105 78-79 0.20670233695111762 1105 80-81 0.23226692115604664 1105 82-83 0.20106456585849486 1105 84-85 0.19422626848507463 1105 86-87 0.2318798461857483 1105 88-89 0.2101203043966393 1105 90-91 0.18353355037471886 1105 92-93 0.217531194971329 1105 94-95 0.18084075900607388 1105 96-97 0.1706296945394996 1105 98-99 0.1817293729143259 1105 100 0.16841547245450528 1106 1 0.010819790808923813 1106 2 0.01941031231137913 1106 3 0.034929487917096935 1106 4 0.03121670428853207 1106 5 0.021882918367730042 1106 6 0.020776561147812345 1106 7 0.03890635144496457 1106 8 0.025951063995748314 1106 9 0.016757742831828182 1106 10-11 -0.00490591862953238 1106 12-13 0.003567883505688485 1106 14-15 0.0197565716820165 1106 16-17 0.00517396552783822 1106 18-19 0.049566318517477725 1106 20-21 0.01592557738392486 1106 22-23 0.06429773299570485 1106 24-25 0.035774107595266 1106 26-27 -0.006807674554401899 1106 28-29 -0.044204030458686816 1106 30-31 0.009354222911653665 1106 32-33 0.028275326727602135 1106 34-35 0.06924666063338947 1106 36-37 0.04341379329779471 1106 38-39 0.03751738496700341 1106 40-41 0.08014117989525005 1106 42-43 0.1342201032334387 1106 44-45 0.08748625197353732 1106 46-47 0.16279235447527896 1106 48-49 0.084462504630892 1106 50-51 0.06586628209302603 1106 52-53 0.08352705556138318 1106 54-55 0.05508243155520631 1106 56-57 0.07729401601424968 1106 58-59 0.03469871709950212 1106 60-61 0.07771601350581392 1106 62-63 0.06975184428858228 1106 64-65 0.0035586869327275394 1106 66-67 0.05715578226365636 1106 68-69 0.049228747418929686 1106 70-71 0.06032314906368441 1106 72-73 0.05798400779105606 1106 74-75 0.11912230425306802 1106 76-77 -0.012869587363574908 1106 78-79 0.021330690064786495 1106 80-81 0.0743026776763358 1106 82-83 0.04195933456352918 1106 84-85 0.03676366197756664 1106 86-87 0.052677762180259435 1106 88-89 -0.0030243766867386057 1106 90-91 -0.005460568344147987 1106 92-93 0.04390221379874504 1106 94-95 0.04876545240704644 1106 96-97 0.05518933130424841 1106 98-99 0.047018321052526346 1106 100 0.05165854713992957 1107 1 -0.045146856822420034 1107 2 -0.036247195775075625 1107 3 -0.02706353633164582 1107 4 -0.006997711195531053 1107 5 -0.10216772607284241 1107 6 -0.08317576414585659 1107 7 -0.06571826522174717 1107 8 -0.05888368614872519 1107 9 -0.029764107820824393 1107 10-11 -0.04896379913868998 1107 12-13 0.010796942958734235 1107 14-15 0.017767161651335073 1107 16-17 0.02440645988733081 1107 18-19 -0.009790153240402333 1107 20-21 0.01270417592112949 1107 22-23 0.011921757333645644 1107 24-25 1.3540127874733798E-4 1107 26-27 0.028429018058005795 1107 28-29 -0.027957133111996768 1107 30-31 -0.04527450764399532 1107 32-33 -0.023005227420618723 1107 34-35 0.0061259932237263115 1107 36-37 0.03077315083300647 1107 38-39 -0.0028557918258655945 1107 40-41 -0.006599392264533321 1107 42-43 -0.026026174460326956 1107 44-45 0.019079504422272464 1107 46-47 -0.04919327647647265 1107 48-49 -0.05188943096021603 1107 50-51 -0.07100290443111135 1107 52-53 -0.013427944090828703 1107 54-55 -0.026492093412741724 1107 56-57 -0.05619907206718722 1107 58-59 -0.09837657083328821 1107 60-61 -0.08670689183397684 1107 62-63 -0.007195853147180742 1107 64-65 -0.114778345847661 1107 66-67 -0.0938205959743641 1107 68-69 -0.11743000694435324 1107 70-71 -0.1444732638083437 1107 72-73 -0.11221033771567335 1107 74-75 -0.05240035416689892 1107 76-77 -0.12164998237526703 1107 78-79 -0.10483311181378241 1107 80-81 -0.11094575363955528 1107 82-83 -0.0718524585542113 1107 84-85 -0.08098903066068885 1107 86-87 -0.09556232020538857 1107 88-89 -0.05837161308199512 1107 90-91 -0.023229095765771035 1107 92-93 -0.0825047705619113 1107 94-95 -0.13590390515513207 1107 96-97 -0.011335432907841891 1107 98-99 0.005225369189915341 1107 100 0.014642425834495043 1108 1 0.0674711943534092 1108 2 0.05553230538225051 1108 3 0.0059339818338415284 1108 4 0.016433569722273944 1108 5 0.0021939961204751057 1108 6 0.01111280449740093 1108 7 0.04565453450353374 1108 8 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-0.111876492092712 2214 5 -0.11568633432707287 2214 6 -0.07114517285144473 2214 7 -0.10555400431540818 2214 8 -0.08047610714851317 2214 9 -0.0967713347298158 2214 10-11 -0.08118432531819053 2214 12-13 -0.07894430556279275 2214 14-15 -0.13744477157230506 2214 16-17 -0.09505249266960902 2214 18-19 -0.09996447033590528 2214 20-21 -0.1536931665626824 2214 22-23 -0.1725684228735389 2214 24-25 -0.18527427799021012 2214 26-27 -0.21438249443226454 2214 28-29 -0.1519720105630995 2214 30-31 -0.10052720705908769 2214 32-33 -0.16822884340331967 2214 34-35 -0.20854638093629063 2214 36-37 -0.2037081376097163 2214 38-39 -0.24947926603872617 2214 40-41 -0.1887791522525788 2214 42-43 -0.2253866470445729 2214 44-45 -0.23124309105772767 2214 46-47 -0.2256744122300418 2214 48-49 -0.32201668173765086 2214 50-51 -0.23460194634065346 2214 52-53 -0.3319824399594964 2214 54-55 -0.2789539681319724 2214 56-57 -0.3253979546725674 2214 58-59 -0.33412599479419924 2214 60-61 -0.42930889849566256 2214 62-63 -0.3652772097336623 2214 64-65 -0.26526442519573123 2214 66-67 -0.3740522553225958 2214 68-69 -0.31260041959244944 2214 70-71 -0.2619557073875498 2214 72-73 -0.3457864332093763 2214 74-75 -0.30528579403285505 2214 76-77 -0.2479387954934893 2214 78-79 -0.43266038141683794 2214 80-81 -0.3855227605107956 2214 82-83 -0.34048589290252096 2214 84-85 -0.332704178398771 2214 86-87 -0.26767438271642163 2214 88-89 -0.39118686804229696 2214 90-91 -0.29491797825107824 2214 92-93 -0.28915007887053434 2214 94-95 -0.31104972442871315 2214 96-97 -0.413308000058791 2214 98-99 -0.42375259667794296 2214 100 -0.5714759780337495 2215 1 -0.031626489839162275 2215 2 -0.059959645270623696 2215 3 -0.08727891574363156 2215 4 -0.07281641961680663 2215 5 -0.06737548416094086 2215 6 -0.06866429102858973 2215 7 -0.04924444693302377 2215 8 -0.06583278171334683 2215 9 -0.08087625260314013 2215 10-11 -0.08862704923237885 2215 12-13 -0.11084066556855277 2215 14-15 -0.1225190026974019 2215 16-17 -0.15591673978585163 2215 18-19 -0.14958075360615908 2215 20-21 -0.12490425312467579 2215 22-23 -0.1789146731927076 2215 24-25 -0.13930844184034896 2215 26-27 -0.11447186360007322 2215 28-29 -0.09146569118040304 2215 30-31 -0.052228810134465675 2215 32-33 -0.11204358309919371 2215 34-35 -0.1536003652479465 2215 36-37 -0.10214514793747043 2215 38-39 -0.17384500381778167 2215 40-41 -0.1703836161219101 2215 42-43 -0.1209879268063574 2215 44-45 -0.1356547548225535 2215 46-47 -0.1902932822444825 2215 48-49 -0.18072008553214403 2215 50-51 -0.11835636920253023 2215 52-53 -0.18281121789055277 2215 54-55 -0.16522745622020807 2215 56-57 -0.2198986424445053 2215 58-59 -0.22499906311994522 2215 60-61 -0.19216652715530103 2215 62-63 -0.18028169878402878 2215 64-65 -0.12684519456679055 2215 66-67 -0.14860720392699278 2215 68-69 -0.10819265849700344 2215 70-71 -0.06628796457778208 2215 72-73 -0.034215194980625085 2215 74-75 -0.06939515977258992 2215 76-77 -0.13431416482524838 2215 78-79 -0.13194866395598126 2215 80-81 -0.13837553338681374 2215 82-83 -0.03814387943105402 2215 84-85 -0.03487716117956552 2215 86-87 -0.09729666315401175 2215 88-89 -0.14796835424940014 2215 90-91 -0.1274238410802937 2215 92-93 -0.10142683735649172 2215 94-95 -0.07095499350306511 2215 96-97 -0.1020580162381961 2215 98-99 -0.12124139562457259 2215 100 -0.07022865351953556 2216 1 -0.057231637747513275 2216 2 -0.07506660686849642 2216 3 -0.1358479903266101 2216 4 -0.07829613424708981 2216 5 -0.0613749149156817 2216 6 -0.03837833553314596 2216 7 -0.10001455766639822 2216 8 -0.09383496449688522 2216 9 -0.14355992989208488 2216 10-11 -0.12096175429840628 2216 12-13 -0.11115815766483905 2216 14-15 -0.19799070168116373 2216 16-17 -0.18740546674017367 2216 18-19 -0.17852925772802308 2216 20-21 -0.16229650042649268 2216 22-23 -0.16582834402255742 2216 24-25 -0.21133835154104474 2216 26-27 -0.2532184380978819 2216 28-29 -0.2531077219885276 2216 30-31 -0.19925100630719328 2216 32-33 -0.14817855948503933 2216 34-35 -0.13664871791188915 2216 36-37 -0.1555840895177809 2216 38-39 -0.20238054490142332 2216 40-41 -0.18635803817758756 2216 42-43 -0.16438854085448895 2216 44-45 -0.04695412006352484 2216 46-47 -0.12595131178994734 2216 48-49 -0.1699658458970177 2216 50-51 -0.046603672199175605 2216 52-53 -0.03737614999803185 2216 54-55 -0.013761376530965208 2216 56-57 -0.04146850881065234 2216 58-59 -0.07235148207825404 2216 60-61 -0.08881479393414793 2216 62-63 -0.030781133802022964 2216 64-65 -0.008603122632159454 2216 66-67 0.011062243669982763 2216 68-69 0.04394832489909817 2216 70-71 0.02775298025714079 2216 72-73 -0.005596335983327094 2216 74-75 0.034617090996668765 2216 76-77 0.03935314442932025 2216 78-79 0.08648445875069655 2216 80-81 0.05277980822622652 2216 82-83 0.0904780044429856 2216 84-85 0.06993012095188078 2216 86-87 0.0330482008470554 2216 88-89 0.060970771093558085 2216 90-91 0.03722528447563889 2216 92-93 0.020270902490302944 2216 94-95 -0.017460823468518072 2216 96-97 0.003953476890799834 2216 98-99 0.06160733732118473 2216 100 0.044630751722884554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30.0 3 6.0 4 16.0 5 44.0 6 90.0 7 200.0 8 433.0 9 863.0 10 1567.0 11 2627.0 12 4224.0 13 6508.0 14 7640.0 15 10114.0 16 12235.0 17 17160.0 18 19819.0 19 10810.0 20 7364.0 21 6926.0 22 6804.0 23 7679.0 24 8714.0 25 10623.0 26 13363.0 27 17068.0 28 22012.0 29 28519.0 30 36983.0 31 47152.0 32 60807.0 33 79446.0 34 109849.0 35 166873.0 36 297999.0 37 709060.0 38 1505065.0 39 881784.0 40 3724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.611226972161056 20.791129843127457 13.93702808272746 23.66061510198403 2 14.925040385449059 20.82557343213607 41.09444748896996 23.154938693444908 3 17.452889233904227 26.27951094075979 31.05288438212605 25.214715443209933 4 11.6590667438748 17.431943352455043 40.537617009983286 30.371372893686875 5 11.782807050667724 38.423702583415015 37.010399620834775 12.78309074508249 6 29.509919103817694 39.85762931757022 18.003215275737674 12.629236302874414 7 25.56144509566601 33.367782654863646 23.84931381560928 17.221458433861063 8 24.02143523398392 37.15730856329834 21.151064302063105 17.67019190065463 9 24.503559630944373 16.364210638162124 22.246486521629123 36.88574320926438 10-11 22.925170191797438 27.159463270411642 30.179595178530818 19.735771359260102 12-13 23.971362379312016 24.61049924797438 30.21751734510698 21.200621027606616 14-15 21.01534691330861 26.635347607113392 27.617760626190353 24.731544853387646 16-17 20.12174324389889 30.1330478870506 28.12216777448935 21.623041094561156 18-19 19.915074402687985 28.729016193187295 30.8680699719191 20.48783943220562 20-21 21.164875551889768 28.044211828635195 30.424045412643736 20.366867206831305 22-23 20.65720421843831 27.919462208064694 30.548829760110465 20.87450381338653 24-25 20.60135437552081 28.10170309750024 30.428812804828496 20.868129722150456 26-27 20.378271053742083 27.974271028027182 30.564744117928537 21.0827138003022 28-29 20.42282660026253 28.054640273383324 30.605314651993904 20.917218474360244 30-31 20.728683916312338 28.52618837125607 30.123767716333838 20.621359996097755 32-33 20.338071873637737 28.149438425404842 30.692936449189794 20.819553251767626 34-35 20.54155695440459 27.996894933689752 30.58929528632201 20.87225282558365 36-37 20.903873643659338 28.360243982988067 29.98754946183149 20.748332911521103 38-39 20.743026956514143 28.47864773864421 30.09838744274665 20.679937862095006 40-41 20.619198885491997 28.231100738026676 30.243041513894404 20.906658862586923 42-43 20.630818778879306 28.100281298354602 30.139748964330455 21.129150958435638 44-45 20.791878726850307 28.34355872879145 29.946691254526815 20.917871289831425 46-47 20.92440977130382 28.62311350762749 29.567457585008317 20.88501913606037 48-49 20.899915375381028 28.502605215477377 29.465646965887153 21.131832443254435 50-51 20.900876268406922 28.517947083757345 29.323349313603696 21.257827334232037 52-53 20.991392664004334 28.47102183942596 29.291919914584096 21.24566558198561 54-55 21.075545199208396 28.81906306461483 29.10044329182236 21.00494844435441 56-57 20.91034303739199 28.486932241002556 29.198188532588887 21.404536189016568 58-59 20.703751853705434 28.17549778040532 29.659505272613874 21.461245093275377 60-61 21.011578852885737 28.574027757224957 29.200991225495308 21.213402164393997 62-63 20.803711190398648 28.566025674848728 29.243467117404787 21.386796017347837 64-65 20.836910118526685 28.2665434275397 29.357391648729674 21.539154805203943 66-67 20.741523049223005 28.322236448789933 29.24917361647168 21.687066885515378 68-69 21.242527557248096 28.47505886496985 29.092613410587592 21.189800167194466 70-71 21.11922250904436 28.326071404399077 29.0909452588592 21.463760827697364 72-73 20.92272382353398 28.43721374342095 29.24705301277772 21.393009420267354 74-75 20.94760146097595 28.488460948780446 29.056437667872505 21.5074999223711 76-77 21.02206780024902 28.47928251599729 28.963710364594487 21.534939319159207 78-79 21.13223077442777 28.511739548689857 29.043809996049436 21.312219680832932 80-81 21.019401165646777 28.358439416084956 29.099492379629687 21.52266703863858 82-83 20.838446928943682 28.43129644234448 29.25178443159095 21.478472197120894 84-85 20.80058978415821 28.335232810940603 29.125747843498573 21.738429561402615 86-87 20.885045789335216 28.50620670766668 28.9887410671042 21.6200064358939 88-89 20.68756974431129 28.308330503129397 29.131859201397315 21.872240551162 90-91 20.6692739323446 28.257154525673617 29.13326468873345 21.940306853248334 92-93 20.748998289505188 28.405773229288545 28.976924694804886 21.868303786401384 94-95 20.462568531366745 28.122070738925814 29.24685847363059 22.168502256076852 96-97 20.470840813158024 28.174724661588474 29.162619475037598 22.191815050215904 98-99 20.45173693658726 28.23704575226821 29.06101111057203 22.25020620057251 100 20.52753384115278 28.131555965261267 28.950196497016158 22.39071369656979 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 57.0 1 45.5 2 94.5 3 156.0 4 116.5 5 103.0 6 209.0 7 487.5 8 921.0 9 1179.0 10 1084.5 11 1010.0 12 1174.0 13 1411.5 14 1623.0 15 1890.5 16 2188.5 17 2531.5 18 3128.5 19 3889.5 20 4897.5 21 6442.0 22 9055.5 23 13098.0 24 18293.5 25 24639.5 26 33099.0 27 44137.0 28 57534.0 29 71364.0 30 84797.0 31 98242.0 32 110889.0 33 124044.5 34 136959.5 35 145680.5 36 151014.0 37 156537.5 38 160952.0 39 162451.5 40 162861.5 41 161185.0 42 155676.5 43 150032.0 44 145023.5 45 139199.5 46 133869.5 47 128483.5 48 122519.5 49 117338.5 50 112750.5 51 106260.0 52 98174.5 53 91507.0 54 86660.5 55 81453.0 56 74509.5 57 67558.0 58 60927.5 59 53725.5 60 46134.5 61 38588.5 62 32042.5 63 26481.0 64 21509.5 65 16998.0 66 13333.5 67 10137.0 68 7628.5 69 5973.0 70 4283.0 71 3017.0 72 2374.5 73 1913.0 74 1467.5 75 1055.0 76 723.0 77 508.5 78 367.5 79 252.0 80 154.5 81 66.0 82 33.0 83 19.5 84 11.0 85 3.5 86 0.5 87 2.5 88 2.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09123768861287662 2 0.0011401678715249137 3 0.0 4 2.1833001795157925E-4 5 0.03806219979622532 6 7.277667265052642E-5 7 0.0049245548493522875 8 3.638833632526321E-4 9 7.277667265052642E-5 10-11 3.0323613604386006E-4 12-13 0.0 14-15 7.277667265052642E-5 16-17 0.0 18-19 8.490611809228083E-5 20-21 0.0 22-23 2.6684779971859686E-4 24-25 1.2129445441754403E-4 26-27 0.0018558051525884238 28-29 0.008939401290572996 30-31 0.03631555965261268 32-33 0.025823589345495123 34-35 0.028940856824026008 36-37 0.026514967735675125 38-39 0.025326282082383196 40-41 0.031014991994566008 42-43 0.023118723011983894 44-45 0.022439474067245647 46-47 0.01605938576488283 48-49 0.01135316093348212 50-51 0.0084057056911358 52-53 0.00608898161176071 54-55 0.004791130949492989 56-57 0.003093008587647373 58-59 0.001237203435058949 60-61 7.277667265052642E-4 62-63 0.0017587695890543885 64-65 0.0032143030420649166 66-67 5.336955994371937E-4 68-69 0.0013099801077094755 70-71 4.124011450196497E-4 72-73 5.822133812042114E-4 74-75 5.822133812042114E-4 76-77 4.245305904614041E-4 78-79 3.638833632526321E-5 80-81 6.064722720877201E-4 82-83 4.609189267866673E-4 84-85 3.396244723691233E-4 86-87 1.5768279074280725E-4 88-89 0.0 90-91 3.638833632526321E-5 92-93 2.4258890883508803E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4122200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.772166016222904 #Duplication Level Percentage of deduplicated Percentage of total 1 70.15667554752243 41.23259782425274 2 16.74650943778456 19.684572657394355 3 6.1433964887236305 10.831821550162381 4 2.805054523371716 6.594365205286381 5 1.4423203050049422 4.238414420715985 6 0.8245072069055543 2.9074844667495294 7 0.5287399811572299 2.17526357663911 8 0.3298743444477114 1.5509943789098877 9 0.21420117671941638 1.1330160407121457 >10 0.7727307237207747 7.162038478257031 >50 0.02599033922261181 1.0480455118685617 >100 0.009462633708470211 0.9502540959081712 >500 3.7197124695596823E-4 0.1338898747108564 >1k 1.239903478840956E-4 0.21531191711361736 >5k 4.133011596136516E-5 0.14193000131936664 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5843 0.14174469943234194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4950 0.1200815098733686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.4258890883508807E-5 0.0 3 0.0 0.0 0.0 2.4258890883508807E-5 0.0 4 0.0 0.0 0.0 7.277667265052642E-5 0.0 5 0.0 0.0 0.0 7.277667265052642E-5 0.0 6 0.0 0.0 0.0 7.277667265052642E-5 0.0 7 0.0 0.0 0.0 7.277667265052642E-5 0.0 8 0.0 0.0 0.0 7.277667265052642E-5 0.0 9 0.0 0.0 0.0 1.2129445441754403E-4 0.0 10-11 0.0 0.0 0.0 1.2129445441754403E-4 0.0 12-13 0.0 1.2129445441754403E-5 0.0 1.4555334530105284E-4 0.0 14-15 0.0 2.4258890883508807E-5 0.0 1.9407112706807045E-4 0.0 16-17 0.0 2.4258890883508807E-5 0.0 1.9407112706807045E-4 0.0 18-19 0.0 3.638833632526321E-5 0.0 1.9407112706807045E-4 0.0 20-21 0.0 7.277667265052642E-5 0.0 1.9407112706807045E-4 0.0 22-23 0.0 7.277667265052642E-5 0.0 2.3045946339333366E-4 0.0 24-25 0.0 7.277667265052642E-5 0.0 2.6684779971859686E-4 0.0 26-27 0.0 7.277667265052642E-5 0.0 3.881422541361409E-4 0.0 28-29 0.0 7.277667265052642E-5 0.0 0.0010310028625491242 0.0 30-31 0.0 7.277667265052642E-5 0.0 0.003917810877686672 0.0 32-33 0.0 7.277667265052642E-5 0.0 0.010977148124787735 0.0 34-35 0.0 8.490611809228082E-5 0.0 0.02123865896851196 0.0 36-37 0.0 1.2129445441754403E-4 0.0 0.03897190820435689 0.0 38-39 0.0 1.5768279074280725E-4 0.0 0.06991412352627238 0.0 40-41 0.0 1.8194168162631604E-4 0.0 0.10653291931492892 0.0 42-43 0.0 2.4258890883508807E-4 0.0 0.14429188297511036 0.0 44-45 0.0 2.4258890883508807E-4 0.0 0.1883702877104459 0.0 46-47 0.0 2.4258890883508807E-4 0.0 0.2410848576003105 0.0 48-49 0.0 2.4258890883508807E-4 0.0 0.3023628159720537 0.0 50-51 0.0 2.4258890883508807E-4 0.0 0.3694629081558391 0.0 52-53 0.0 2.4258890883508807E-4 0.0 0.43691475425743537 0.0 54-55 0.0 2.4258890883508807E-4 0.0 0.5090970840813158 0.0 56-57 0.0 2.4258890883508807E-4 0.0 0.5962714084712046 0.0 58-59 0.0 2.4258890883508807E-4 0.0 0.6970792295376256 0.0 60-61 0.0 2.4258890883508807E-4 0.0 0.802702440444423 0.0 62-63 0.0 2.4258890883508807E-4 0.0 0.9117340255203532 0.0 64-65 0.0 2.4258890883508807E-4 0.0 1.0309422153219154 0.0 66-67 0.0 2.6684779971859686E-4 0.0 1.1665130270244044 0.0 68-69 0.0 2.6684779971859686E-4 0.0 1.3176823055649896 0.0 70-71 0.0 2.6684779971859686E-4 0.0 1.4816845373829508 0.0 72-73 0.0 2.6684779971859686E-4 0.0 1.653255543156567 0.0 74-75 0.0 2.6684779971859686E-4 0.0 1.826900684100723 0.0 76-77 0.0 2.6684779971859686E-4 0.0 2.0138275678036 0.0 78-79 0.0 2.6684779971859686E-4 0.0 2.231854349619135 0.0 80-81 0.0 2.7897724516035124E-4 0.0 2.4704162825675606 0.0 82-83 0.0 2.911066906021057E-4 0.0 2.7174203095434475 0.0 84-85 0.0 2.911066906021057E-4 0.0 2.96563728106351 0.0 86-87 0.0 2.911066906021057E-4 0.0 3.2260686041434186 0.0 88 0.0 2.911066906021057E-4 0.0 3.4406142351171702 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5135 0.0 52.77954 1 GTATCAA 9645 0.0 40.686222 1 TCAACGC 11035 0.0 34.591274 4 ATCAACG 11100 0.0 34.431065 3 CAACGCA 11260 0.0 33.900063 5 AACGCAG 11820 0.0 32.28496 6 TATCAAC 12110 0.0 31.67628 2 ACGCAGA 13360 0.0 28.387608 7 CGCAGAG 13565 0.0 27.888296 8 GTACATG 9045 0.0 25.854218 1 TACATGG 8720 0.0 25.391748 2 GCAGAGT 15695 0.0 24.102644 9 ACATGGG 9185 0.0 23.389446 3 GAGTACT 10865 0.0 21.690586 12-13 AGAGTAC 13870 0.0 21.531246 10-11 CAGAGTA 15305 0.0 20.418247 10-11 GGTACCG 240 1.5837395E-7 19.581703 6 GTATACG 345 6.002665E-11 19.093805 1 AGTACTT 12170 0.0 18.882017 12-13 GTACTTT 12570 0.0 18.598927 14-15 >>END_MODULE