FastQCFastQC Report
Wed 25 May 2016
SRR1033191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677367
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33300.032.9682121
GGTATCA21050.032.232511
TCAACGC32500.031.9448184
ATCAACG33450.031.3260573
CAACGCA34000.030.5343465
AACGCAG36250.028.7574146
GTACATG75250.028.2392881
TACATGG76800.026.1415652
TATCAAC40950.025.7192922
TAGCGCG1301.0925507E-525.295674
ACATGGG80050.024.2429643
ACGCAGA44250.023.133867
CGCAGAG43900.022.9974068
CCGATCG1252.439497E-422.5395959
CATGGGG52150.020.9890654
CGCGCGT1656.911093E-519.9213587
GAGTACA57350.019.307151
GTATTAG11700.018.9276081
GTATAGA11500.017.6179081
AGTACAT56850.017.6162742
Produced by FastQC (version 0.11.5)