##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033191_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2677367
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.698654312240347	33.0	31.0	34.0	30.0	34.0
2	31.784279107048082	34.0	31.0	34.0	30.0	34.0
3	31.96685699046862	34.0	31.0	34.0	30.0	34.0
4	35.34766806343695	37.0	35.0	37.0	33.0	37.0
5	35.43059991402001	37.0	35.0	37.0	35.0	37.0
6	35.4880795199164	37.0	36.0	37.0	35.0	37.0
7	35.46256938253142	37.0	37.0	37.0	35.0	37.0
8	35.45241052123224	37.0	37.0	37.0	35.0	37.0
9	37.13860258978317	39.0	38.0	39.0	35.0	39.0
10-11	37.12656781830806	39.0	38.0	39.0	35.0	39.0
12-13	37.080515670806435	39.0	38.0	39.0	35.0	39.0
14-15	38.55238168693347	41.0	39.0	41.0	35.0	41.0
16-17	38.56041215119183	41.0	39.0	41.0	35.0	41.0
18-19	38.54664676153848	41.0	39.0	41.0	35.0	41.0
20-21	38.580465808385625	41.0	39.0	41.0	34.5	41.0
22-23	38.5423587801	41.0	39.0	41.0	34.0	41.0
24-25	38.5285282144734	41.0	39.0	41.0	34.0	41.0
26-27	38.48365483700964	41.0	39.0	41.0	34.0	41.0
28-29	38.45304939517071	40.5	39.0	41.0	34.0	41.0
30-31	38.451339506313474	41.0	39.0	41.0	34.0	41.0
32-33	38.51815384293599	41.0	39.0	41.0	34.0	41.0
34-35	38.48811257477962	41.0	39.0	41.0	34.0	41.0
36-37	38.41056343788506	41.0	39.0	41.0	34.0	41.0
38-39	38.335012906336715	41.0	39.0	41.0	34.0	41.0
40-41	38.27652148547435	41.0	39.0	41.0	33.5	41.0
42-43	38.16528608143747	40.5	38.0	41.0	33.0	41.0
44-45	38.034961774011556	40.0	38.0	41.0	33.0	41.0
46-47	37.910558582368424	40.0	38.0	41.0	33.0	41.0
48-49	37.79151588108765	40.0	38.0	41.0	33.0	41.0
50-51	37.668489041659214	40.0	38.0	41.0	32.5	41.0
52-53	37.521644959394806	40.0	37.0	41.0	32.0	41.0
54-55	37.370825516262805	40.0	37.0	41.0	32.0	41.0
56-57	37.2145320383795	40.0	36.5	41.0	32.0	41.0
58-59	37.016635746985756	40.0	36.0	41.0	31.0	41.0
60-61	36.79274339304249	39.5	35.5	41.0	31.0	41.0
62-63	36.53022615128968	39.0	35.0	41.0	31.0	41.0
64-65	36.25996828227135	39.0	35.0	41.0	31.0	41.0
66-67	35.96663718496568	38.0	35.0	40.5	30.0	41.0
68-69	35.65664232807829	37.5	35.0	40.0	30.0	41.0
70-71	35.30695847823627	37.0	35.0	40.0	30.0	41.0
72-73	34.94159429768126	36.5	34.5	39.0	29.5	41.0
74-75	34.57089595860411	36.0	34.0	39.0	29.0	41.0
76-77	34.239216551186296	35.5	34.0	38.5	29.0	40.0
78-79	33.86743524514944	35.0	34.0	37.0	29.0	39.0
80-81	33.507892082034324	35.0	34.0	37.0	29.0	39.0
82-83	33.29178909727355	35.0	34.0	36.5	29.0	39.0
84-85	33.02392611845892	35.0	34.0	36.0	29.0	37.0
86-87	32.77373012366254	35.0	34.0	36.0	28.0	37.0
88-89	32.420494276653145	35.0	34.0	35.5	27.0	37.0
90-91	32.24303093300246	35.0	34.0	35.0	27.0	36.0
92-93	32.07391963821172	35.0	34.0	35.0	27.0	36.0
94-95	31.912702479712344	35.0	33.5	35.0	27.0	36.0
96-97	31.751286431781672	35.0	33.0	35.0	26.0	36.0
98-99	31.57033159817089	35.0	33.0	35.0	25.5	35.0
100	31.415636705763536	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1306838546971818
1101	2	0.13828633982486593
1101	3	0.11410777348084977
1101	4	0.13926037546087855
1101	5	0.12150344890602582
1101	6	0.08461235492227104
1101	7	0.14429880183559618
1101	8	0.12794044486688705
1101	9	0.19850693522838725
1101	10-11	0.19806488664691102
1101	12-13	0.25788900747174637
1101	14-15	0.25595052738186297
1101	16-17	0.1853674976401507
1101	18-19	0.16427812281710885
1101	20-21	0.19905653981676608
1101	22-23	0.21849156911867595
1101	24-25	0.22051274890835515
1101	26-27	0.21352038093377956
1101	28-29	0.2982909512078038
1101	30-31	0.28656119221837884
1101	32-33	0.3295591962572857
1101	34-35	0.30316031593427084
1101	36-37	0.24077774631906834
1101	38-39	0.2982495832241341
1101	40-41	0.2784003559223649
1101	42-43	0.3149860145147798
1101	44-45	0.2960160375868739
1101	46-47	0.2936723073032539
1101	48-49	0.2888076409741487
1101	50-51	0.26141558028317036
1101	52-53	0.19179417849062474
1101	54-55	0.1302680719405629
1101	56-57	0.2021055909298468
1101	58-59	0.18690112677524695
1101	60-61	0.20315025113301743
1101	62-63	0.25333504086783165
1101	64-65	0.18674110783516085
1101	66-67	0.1406653414931469
1101	68-69	0.1453854142888602
1101	70-71	0.11858485758374826
1101	72-73	0.1659888561724827
1101	74-75	0.17342474919645667
1101	76-77	0.18094895299740443
1101	78-79	0.25456956279543164
1101	80-81	0.1820713569131911
1101	82-83	0.19666677714546665
1101	84-85	0.2263407019070769
1101	86-87	0.2349910401693336
1101	88-89	0.2982961134004185
1101	90-91	0.34781947592011875
1101	92-93	0.37238264094781925
1101	94-95	0.4420952952365944
1101	96-97	0.3760529358807503
1101	98-99	0.4646340134047442
1101	100	0.563588925733093
1102	1	0.2230941384651608
1102	2	0.19511855311143123
1102	3	0.23253877928393152
1102	4	0.22918744193186313
1102	5	0.16218733437940358
1102	6	0.1239315219948054
1102	7	0.173955869181313
1102	8	0.195604156539531
1102	9	0.284771810540164
1102	10-11	0.2632666552516909
1102	12-13	0.2380159977010834
1102	14-15	0.27063366915713516
1102	16-17	0.18150883483523472
1102	18-19	0.1628638005452956
1102	20-21	0.253935339705464
1102	22-23	0.24708496100473099
1102	24-25	0.25321807851671707
1102	26-27	0.30048939363316407
1102	28-29	0.33291096143815224
1102	30-31	0.25405514202199697
1102	32-33	0.2310720423279733
1102	34-35	0.31472147358351066
1102	36-37	0.26695300349066287
1102	38-39	0.18996300099923502
1102	40-41	0.24062088868522125
1102	42-43	0.2108996706620445
1102	44-45	0.1811587063111233
1102	46-47	0.17160632407569665
1102	48-49	0.15044491428752593
1102	50-51	0.1699355670794631
1102	52-53	0.17139838423653941
1102	54-55	0.20187197963128511
1102	56-57	0.1494454303616095
1102	58-59	0.20920397028655913
1102	60-61	0.13183939504867936
1102	62-63	0.1173391891625215
1102	64-65	0.14872683214348115
1102	66-67	0.11713104048835987
1102	68-69	0.034360288020373275
1102	70-71	0.05915488453469919
1102	72-73	0.16632758797645408
1102	74-75	0.16292298336344402
1102	76-77	0.09466525125273506
1102	78-79	0.0879656029090512
1102	80-81	0.15952801742077583
1102	82-83	0.07247815860607432
1102	84-85	0.06074199434349481
1102	86-87	0.08688536448577366
1102	88-89	0.2703920641334747
1102	90-91	0.2855216935875191
1102	92-93	0.25696339644573385
1102	94-95	0.19520657025406862
1102	96-97	0.19422400732076284
1102	98-99	0.23770163125623966
1102	100	0.261282489720827
1103	1	0.0637063877197157
1103	2	0.06029181972508013
1103	3	0.11949770518657132
1103	4	0.06660671333352752
1103	5	0.039975620009776947
1103	6	0.04557147377612836
1103	7	0.012189585515855583
1103	8	0.015962364467760892
1103	9	-0.0012558750607425395
1103	10-11	0.09475240965022635
1103	12-13	0.14756307609317787
1103	14-15	0.06837163453980821
1103	16-17	0.09946938016307882
1103	18-19	0.10457174732125907
1103	20-21	0.09545370989814472
1103	22-23	0.05205255004807441
1103	24-25	0.06546292543747256
1103	26-27	0.12429070223041805
1103	28-29	0.11426717771034589
1103	30-31	0.1357467045617824
1103	32-33	0.14229246688528718
1103	34-35	0.15529161543398828
1103	36-37	0.16200895176080365
1103	38-39	0.18612155530663443
1103	40-41	0.16984925789758165
1103	42-43	0.12364207580241526
1103	44-45	-4.1930095372322285E-4
1103	46-47	0.03567533351680652
1103	48-49	0.054938586052465155
1103	50-51	0.01277805796143383
1103	52-53	0.018956489863455772
1103	54-55	0.02699240024384153
1103	56-57	0.021099852029372812
1103	58-59	0.01620632450150339
1103	60-61	0.08926238461356917
1103	62-63	0.15470092644424227
1103	64-65	0.16686624585504717
1103	66-67	0.09940251075662587
1103	68-69	0.05526286627157617
1103	70-71	0.06970878239187783
1103	72-73	0.12472602543597588
1103	74-75	0.1533999396979553
1103	76-77	0.11236977389191338
1103	78-79	0.07310852870281792
1103	80-81	0.09629319745082654
1103	82-83	0.07330631694290446
1103	84-85	0.07128542579917507
1103	86-87	0.023218796818142096
1103	88-89	0.14099162451697822
1103	90-91	0.19743165711125243
1103	92-93	0.09676956007158566
1103	94-95	0.0920482662421982
1103	96-97	0.08865943269777432
1103	98-99	0.19662736118756996
1103	100	0.18682533844319238
1104	1	0.12652649138235716
1104	2	0.03915751834418302
1104	3	0.07786319733063962
1104	4	0.08546729448540447
1104	5	-0.02095217839554664
1104	6	-0.01873969431105138
1104	7	0.03061373160504388
1104	8	0.003744311664036104
1104	9	0.024187756856051124
1104	10-11	0.05618429055294882
1104	12-13	0.032807066911274774
1104	14-15	-0.010104778639913548
1104	16-17	0.038310823371340064
1104	18-19	-0.032104844916602815
1104	20-21	-0.0032690988169505886
1104	22-23	0.021325126564420316
1104	24-25	-0.04940581838067004
1104	26-27	0.0668749087498881
1104	28-29	0.046805760133331376
1104	30-31	0.11906560426907475
1104	32-33	0.023666755657473004
1104	34-35	-0.08377869671621596
1104	36-37	0.017174982369930092
1104	38-39	0.014758920548153753
1104	40-41	0.0024188631360786417
1104	42-43	0.029489294390458554
1104	44-45	0.017562721780407742
1104	46-47	0.022383690376713616
1104	48-49	-0.06876720269313807
1104	50-51	-0.04506814964074124
1104	52-53	-0.030803494137472853
1104	54-55	-0.0784439905765737
1104	56-57	-0.09727507536173619
1104	58-59	-0.10946232428556613
1104	60-61	-0.11356475656445042
1104	62-63	-0.060570501901942464
1104	64-65	-0.13490215442350717
1104	66-67	-0.06842356960476792
1104	68-69	-0.1933802098824131
1104	70-71	-0.17055274466645898
1104	72-73	-0.1233723385113521
1104	74-75	-0.10222017631259916
1104	76-77	-0.2246729580904514
1104	78-79	-0.19548338874601967
1104	80-81	-0.13667567268732483
1104	82-83	-0.133632528192706
1104	84-85	-0.10679777668680401
1104	86-87	-0.08833204257754801
1104	88-89	0.04805028305264614
1104	90-91	0.03765773712669329
1104	92-93	0.06366496262072019
1104	94-95	0.08434282558017614
1104	96-97	0.12438204175714418
1104	98-99	0.15367573089969255
1104	100	0.08087400747259466
1105	1	0.09821079030229285
1105	2	0.12849325274736145
1105	3	0.13665499595949981
1105	4	0.0823542665403636
1105	5	0.05733538655520931
1105	6	0.08277049143116955
1105	7	0.04074035673763632
1105	8	0.05925168463004127
1105	9	0.15582229830408068
1105	10-11	0.11879134574127193
1105	12-13	0.09507882491185882
1105	14-15	0.11928205841439876
1105	16-17	0.19300221962426178
1105	18-19	0.12519896664554153
1105	20-21	0.14902247193596452
1105	22-23	0.14019742483790765
1105	24-25	0.13725788159725028
1105	26-27	0.1545262406092789
1105	28-29	0.16195612767942436
1105	30-31	0.18536507991305484
1105	32-33	0.18811990522087285
1105	34-35	0.18870981986196256
1105	36-37	0.22814977197328545
1105	38-39	0.19826672706092552
1105	40-41	0.2372129474111091
1105	42-43	0.20713633519244468
1105	44-45	0.1778374163909362
1105	46-47	0.21961444905201688
1105	48-49	0.14725361810690885
1105	50-51	0.16257300905931515
1105	52-53	0.18533842497503628
1105	54-55	0.13343264837789803
1105	56-57	0.06999606379203271
1105	58-59	0.043011585834449306
1105	60-61	-0.02492256181893282
1105	62-63	0.028011835585765255
1105	64-65	0.024788996285934672
1105	66-67	0.07057122622502021
1105	68-69	-0.07085507417860981
1105	70-71	-0.0207835458854575
1105	72-73	0.038766201691132096
1105	74-75	-0.007084466714786686
1105	76-77	0.045305157967362675
1105	78-79	0.017877356207797845
1105	80-81	-0.013831266093838224
1105	82-83	-0.025342832194851894
1105	84-85	-0.029144666487596282
1105	86-87	0.07441395224783065
1105	88-89	0.15817338111241952
1105	90-91	0.1939473865259984
1105	92-93	0.1712190579738504
1105	94-95	0.2388735726766349
1105	96-97	0.1916634982425549
1105	98-99	0.20680345605421735
1105	100	0.20108469472209123
1106	1	0.15667071548591238
1106	2	0.10651906309729142
1106	3	0.032502303891394746
1106	4	0.15503965212334947
1106	5	0.07199654299796521
1106	6	0.06388151079155335
1106	7	0.07140145595096214
1106	8	0.07152394402638151
1106	9	0.08286081771949938
1106	10-11	0.12619139481797959
1106	12-13	0.06316947040988907
1106	14-15	0.13360397013887138
1106	16-17	0.08807668356241294
1106	18-19	0.10057563404627956
1106	20-21	0.10112812955238581
1106	22-23	0.07866761555066404
1106	24-25	0.07721400241128862
1106	26-27	0.11521507944571141
1106	28-29	0.19534401192525053
1106	30-31	0.15390767140598882
1106	32-33	0.07333481129214192
1106	34-35	0.08506528567037464
1106	36-37	0.09631391535446454
1106	38-39	0.11313686959736202
1106	40-41	0.14401460358643448
1106	42-43	0.17794747541916678
1106	44-45	0.18369504597134778
1106	46-47	0.17631677701328385
1106	48-49	0.18399577602523465
1106	50-51	0.144181517447592
1106	52-53	0.14673649556618784
1106	54-55	0.20051180068916352
1106	56-57	0.17982350410768788
1106	58-59	0.14322864448468664
1106	60-61	0.14745195941649314
1106	62-63	0.17257997631934785
1106	64-65	0.11255412102851636
1106	66-67	0.10617798430542535
1106	68-69	0.09436240721704081
1106	70-71	0.11166284022090878
1106	72-73	0.129286386998551
1106	74-75	0.1782193423170213
1106	76-77	0.08079915310150909
1106	78-79	0.07142470828899405
1106	80-81	0.12194805598778657
1106	82-83	0.10161967321589316
1106	84-85	0.04354932201088957
1106	86-87	0.10944650426170455
1106	88-89	0.17996552196983373
1106	90-91	0.23520413249140404
1106	92-93	0.2366677882051107
1106	94-95	0.21261457607752376
1106	96-97	0.11868465765285663
1106	98-99	0.17275675498863308
1106	100	0.2887463069651943
1107	1	0.09884614408336745
1107	2	0.04910700863740658
1107	3	0.1290262479836919
1107	4	0.07679848044612925
1107	5	-0.07585976148854456
1107	6	0.04279108901420159
1107	7	-0.0018161773971456796
1107	8	0.04995812964370572
1107	9	0.06340217478429366
1107	10-11	0.00565468529266866
1107	12-13	0.0014476837417518595
1107	14-15	0.01963188023910334
1107	16-17	-0.007232122604470703
1107	18-19	0.05665279993655048
1107	20-21	-0.013536309849520478
1107	22-23	0.05664505609760084
1107	24-25	0.07163405630505792
1107	26-27	0.143284610126706
1107	28-29	0.11132873863785875
1107	30-31	0.10609064030154514
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2216	20-21	-0.14096702099772074
2216	22-23	-0.12158530176657223
2216	24-25	-0.1433728469342057
2216	26-27	-0.2448433354119075
2216	28-29	-0.133263018714878
2216	30-31	-0.1936891041557942
2216	32-33	-0.09366535470788762
2216	34-35	-0.11740954262628378
2216	36-37	-0.15088053239940535
2216	38-39	-0.10625754093986473
2216	40-41	-0.10532814297042847
2216	42-43	-0.14570209547544977
2216	44-45	-0.09625512054645924
2216	46-47	-0.02198370480521561
2216	48-49	0.07481542499007787
2216	50-51	0.01583919776290088
2216	52-53	0.022292012535999106
2216	54-55	0.03899191086213705
2216	56-57	0.006212703164322875
2216	58-59	-0.05266985763434917
2216	60-61	0.10681895327511626
2216	62-63	0.10306364414397251
2216	64-65	0.10381971595768391
2216	66-67	0.07854046917635316
2216	68-69	0.032592115700275315
2216	70-71	-0.007746962190935847
2216	72-73	0.12150104292921782
2216	74-75	0.16127154425464596
2216	76-77	0.08888767996782576
2216	78-79	0.11937298656948059
2216	80-81	0.12178325885248853
2216	82-83	0.06334947411312442
2216	84-85	0.03121801418479464
2216	86-87	0.13341410590847858
2216	88-89	0.09397091451284467
2216	90-91	0.05710288418578813
2216	92-93	0.042016404489700676
2216	94-95	0.08394556476933346
2216	96-97	0.05643035626980364
2216	98-99	0.06965820797531919
2216	100	0.0669590169194123
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23901.0
3	4349.0
4	2805.0
5	2298.0
6	1978.0
7	2263.0
8	2716.0
9	2875.0
10	3394.0
11	3622.0
12	4018.0
13	4519.0
14	5004.0
15	5139.0
16	5131.0
17	5130.0
18	5287.0
19	5411.0
20	5346.0
21	5675.0
22	6501.0
23	8463.0
24	12931.0
25	18198.0
26	18992.0
27	16913.0
28	17869.0
29	22450.0
30	27574.0
31	33885.0
32	44713.0
33	61298.0
34	86205.0
35	131500.0
36	248942.0
37	539608.0
38	888972.0
39	390637.0
40	855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.025028826375845	19.59770108481606	13.431535998962737	23.94573408984536
2	15.282953954773737	19.870349387272775	40.96806040140458	23.87863625654891
3	18.035949082846894	25.33673299291887	29.84240320351388	26.78491472072036
4	12.05421131217053	16.49420013242321	39.28081940971532	32.17076914569094
5	12.793744803684362	38.160097900922025	35.91144076900801	13.134716526385606
6	31.147520163519495	38.12276064654747	17.790439847118837	12.939279342814197
7	27.525409294142694	32.35618208780167	22.67442859437391	17.443980023681725
8	24.846905021530237	37.14291691275052	20.27275243721079	17.737425628508447
9	24.876058466856964	17.03502639466212	21.086927366955475	37.00198777152545
10-11	23.810896280425958	26.945272033428164	29.079418033200277	20.164413652945605
12-13	24.412193040473184	24.24255781970365	29.04438140766601	22.300867732157155
14-15	21.702694216570997	26.32160735876148	25.909539279832533	26.066159144834987
16-17	21.551196140968585	29.609959513791928	26.543523841099802	22.295320504139678
18-19	21.459145778596984	28.702779800265017	28.310452558551162	21.527621862586845
20-21	22.62027179921983	27.999264771398263	27.84926767607427	21.531195753307642
22-23	22.251094444417465	27.746589468753186	27.85438089691583	22.147935189913518
24-25	22.156666460566075	27.884961499973944	27.771836559866088	22.186535479593893
26-27	21.86928544077405	27.852325049265453	27.918298730772463	22.36009077918803
28-29	21.896804016991748	27.921245623086122	27.953522619487458	22.228427740434668
30-31	22.104949745303607	28.282068107446367	27.62931462004558	21.983667527204446
32-33	21.79812182806543	27.980700852187873	28.096496486249762	22.12468083349694
34-35	22.001495364947683	27.785976405509505	28.060114193882303	22.152414035660513
36-37	22.25814639368613	28.080327534809722	27.634488873083995	22.02703719842015
38-39	21.99631004718343	28.274739736548277	27.78536712191525	21.94358309435304
40-41	21.79889978084519	27.965360316730436	28.05706392320355	22.17867597922082
42-43	21.84391213083965	27.82703329430536	28.058698472754223	22.27035610210077
44-45	21.90001230800493	28.121475339446	27.916310795988004	22.062201556561075
46-47	22.022279476789954	28.210871540485087	27.825084909105	21.94176407361995
48-49	21.960852206321512	27.98965650806671	27.789655686324604	22.259835599287175
50-51	21.887847559368762	27.96807394628652	27.886738559304703	22.25733993504001
52-53	21.926218026728346	27.951039871386	27.94719250787589	22.175549594009766
54-55	21.99064386068022	28.343865479864917	27.758121630558186	21.907369028896685
56-57	21.847511931805876	28.054030593531238	27.84038235564364	22.25807511901925
58-59	21.759258654032013	27.674880499609213	28.164516951360376	22.401343894998398
60-61	22.110078927149544	28.107884908520926	27.71586375570122	22.06617240862831
62-63	21.833474364747016	28.1214335564624	27.835996682477997	22.209095396312588
64-65	21.899406493831496	27.820442907246452	27.929395550011016	22.350755048911033
66-67	21.743295859907644	27.900685741839926	27.838197869174746	22.517820529077685
68-69	22.133197424327626	28.0446415515141	27.797543889202537	22.024617134955736
70-71	22.07709283721743	27.807968798468757	27.85538589278881	22.259552471524998
72-73	21.861533264022587	27.974150963586837	27.91457634133009	22.24973943106049
74-75	21.96520197997428	27.96664185940539	27.857278254365625	22.210877906254698
76-77	21.944999114768574	27.961864827775802	27.722105311911484	22.37103074554415
78-79	22.105488446344307	28.066412439987833	27.75703006365254	22.071069050015325
80-81	22.00093415722938	27.887749485998754	27.888048296787836	22.223268059984026
82-83	21.885566845117527	27.941553826035996	28.005480145863316	22.16739918298316
84-85	21.916662355714614	27.834046336789502	27.83003108180867	22.41926022568721
86-87	22.076621826799713	28.017073138846715	27.72153340766181	22.184771626691766
88-89	21.85622386136047	27.92775308215189	27.79278094457951	22.423242111908134
90-91	21.834567229904234	27.791806470802772	27.900731093348046	22.47289520594495
92-93	22.013558732241062	27.942239447414302	27.768476032091368	22.27572578825327
94-95	21.771474625721645	27.740765859276223	27.97652036198245	22.511239153019684
96-97	21.865473615195814	27.70175717094257	27.95749425375889	22.475274960102723
98-99	21.836666374860375	27.863925340671326	27.796521844330986	22.502886440137317
100	22.0497105608923	27.73182462548755	27.653749144757878	22.564715668862274
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	204.5
2	202.5
3	172.0
4	131.5
5	114.0
6	135.0
7	137.0
8	113.0
9	122.0
10	119.0
11	123.5
12	147.5
13	174.5
14	205.5
15	242.5
16	317.0
17	471.5
18	671.0
19	957.5
20	1419.0
21	2145.0
22	3318.0
23	5317.5
24	7854.0
25	10789.0
26	14869.0
27	20493.5
28	27676.0
29	34945.0
30	41964.0
31	49989.5
32	57641.5
33	64924.0
34	72531.5
35	79253.0
36	84851.5
37	89894.0
38	93658.5
39	95761.0
40	97239.0
41	97895.0
42	96847.5
43	95855.0
44	94670.0
45	93171.5
46	92811.0
47	90975.5
48	88061.5
49	86109.5
50	84598.0
51	82300.0
52	76942.0
53	72315.0
54	69595.5
55	65861.5
56	61299.0
57	55167.0
58	49408.0
59	44766.0
60	40050.5
61	34860.0
62	29931.5
63	25118.0
64	20522.5
65	16869.0
66	13214.5
67	9665.0
68	7145.5
69	5448.0
70	3942.5
71	2846.0
72	2151.5
73	1629.5
74	1271.5
75	914.5
76	592.5
77	388.0
78	255.0
79	164.5
80	106.5
81	55.5
82	36.0
83	29.0
84	24.0
85	20.0
86	14.0
87	11.0
88	7.5
89	3.0
90	2.0
91	2.0
92	3.0
93	2.5
94	3.0
95	3.5
96	2.5
97	2.0
98	2.0
99	1.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3288305264089682
2	0.12164936670990567
3	0.0674169809368682
4	0.041047790609206734
5	0.04452135250789302
6	0.009524282625430133
7	0.007357975204744063
8	0.016209955527202658
9	0.018675063971431635
10-11	0.021737774462746422
12-13	0.016676832126488447
14-15	0.018805789419231656
16-17	0.0197768927457461
18-19	0.0194220665302889
20-21	0.02422155797094683
22-23	0.01635935603897411
24-25	0.025416762065118455
26-27	0.020243769345031894
28-29	0.020094368833260438
30-31	0.020635945688431957
32-33	0.024968560529804094
34-35	0.01600452982351691
36-37	0.010364660504144557
38-39	0.013744847082973683
40-41	0.009860433776915903
42-43	0.008497154107001394
44-45	0.00924415666585866
46-47	0.008945355642315751
48-49	0.004780816376686498
50-51	0.0067416980936868195
52-53	0.007806176740058424
54-55	0.003268136195000536
56-57	0.0025771588280575656
58-59	0.0027825845317433137
60-61	0.0032868112589719677
62-63	0.0025211336361432705
64-65	0.0021289572927432063
66-67	0.0017741310772860055
68-69	0.00319343593911481
70-71	0.0023343829964289544
72-73	0.001960881717000322
74-75	0.0022223326126003646
76-77	0.00377236292222919
78-79	0.0036416374744291688
80-81	0.003268136195000536
82-83	0.002465108444228976
84-85	0.0028946349155719035
86-87	0.002315707932457523
88-89	0.6004780069374127
90-91	0.6107679671856716
92-93	0.6079667075899569
94-95	0.5943152358268403
96-97	0.5917941021906971
98-99	0.5895344194501538
100	0.5916260266149542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2677367.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.58917239238673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70688449199912	48.77727301644627
2	14.007185754646331	17.814106993687783
3	4.367040435588865	8.330894613095518
4	1.8661157006943856	4.746590119823793
5	0.9232722307568497	2.9355058523350386
6	0.5306030174214604	2.024436404804029
7	0.35037358381889006	1.5595976356238432
8	0.2360715098356116	1.200927354869418
9	0.163116538045124	0.9335201092020629
>10	0.7956715559762856	8.692926928785145
>50	0.0413541062162304	1.7992188319870928
>100	0.012252258954894377	1.156727257940571
>500	5.881604582133461E-5	0.028274881399415506
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.735012794286327E-5	0.0
16-17	0.0	0.0	0.0	5.60251919142949E-5	0.0
18-19	0.0	0.0	0.0	7.470025588572653E-5	0.0
20-21	0.0	0.0	0.0	7.470025588572653E-5	0.0
22-23	0.0	0.0	0.0	1.3072544780002145E-4	0.0
24-25	0.0	0.0	0.0	2.801259595714745E-4	0.0
26-27	0.0	0.0	0.0	4.668765992857909E-4	0.0
28-29	0.0	0.0	0.0	8.217028147429919E-4	0.0
30-31	0.0	0.0	0.0	0.0030813855552862196	0.0
32-33	0.0	0.0	0.0	0.009468257433515839	0.0
34-35	0.0	0.0	0.0	0.018320237755974432	0.0
36-37	0.0	0.0	0.0	0.03460489353906282	0.0
38-39	0.0	0.0	0.0	0.06455969614923916	0.0
40-41	0.0	0.0	0.0	0.09925796500815914	0.0
42-43	0.0	0.0	0.0	0.13453516085019349	0.0
44-45	0.0	0.0	0.0	0.1766847802337147	0.0
46-47	0.0	0.0	0.0	0.22789180564338024	0.0
48-49	0.0	0.0	0.0	0.28796948643947584	0.0
50-51	0.0	0.0	0.0	0.3549569409050011	0.0
52-53	0.0	0.0	0.0	0.4221684961381835	0.0
54-55	0.0	0.0	0.0	0.4919945603273664	0.0
56-57	0.0	0.0	0.0	0.578422756387152	0.0
58-59	0.0	0.0	0.0	0.6770084190923396	0.0
60-61	0.0	0.0	0.0	0.7834376086655285	0.0
62-63	0.0	0.0	0.0	0.8907071761174319	0.0
64-65	0.0	0.0	0.0	1.0047744668549363	0.0
66-67	0.0	0.0	0.0	1.1314847759010997	0.0
68-69	0.0	0.0	0.0	1.2782894537805238	0.0
70-71	0.0	0.0	0.0	1.4424619411533794	0.0
72-73	0.0	0.0	0.0	1.6116766957985216	0.0
74-75	0.0	0.0	0.0	1.7809474756355779	0.0
76-77	0.0	0.0	0.0	1.9610871427040073	0.0
78-79	0.0	0.0	0.0	2.1708828113590704	0.0
80-81	0.0	0.0	0.0	2.4031819320997085	0.0
82-83	0.0	0.0	0.0	2.6451734110415197	0.0
84-85	0.0	0.0	0.0	2.8902836256665596	0.0
86-87	0.0	0.0	0.0	3.140230681860201	0.0
88	0.0	0.0	0.0	3.3450027583069484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3330	0.0	32.968212	1
GGTATCA	2105	0.0	32.23251	1
TCAACGC	3250	0.0	31.944818	4
ATCAACG	3345	0.0	31.326057	3
CAACGCA	3400	0.0	30.534346	5
AACGCAG	3625	0.0	28.757414	6
GTACATG	7525	0.0	28.239288	1
TACATGG	7680	0.0	26.141565	2
TATCAAC	4095	0.0	25.719292	2
TAGCGCG	130	1.0925507E-5	25.29567	4
ACATGGG	8005	0.0	24.242964	3
ACGCAGA	4425	0.0	23.13386	7
CGCAGAG	4390	0.0	22.997406	8
CCGATCG	125	2.439497E-4	22.539595	9
CATGGGG	5215	0.0	20.989065	4
CGCGCGT	165	6.911093E-5	19.921358	7
GAGTACA	5735	0.0	19.30715	1
GTATTAG	1170	0.0	18.927608	1
GTATAGA	1150	0.0	17.617908	1
AGTACAT	5685	0.0	17.616274	2
>>END_MODULE