FastQCFastQC Report
Wed 25 May 2016
SRR1033191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677367
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.147234204350767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.13027724626470708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.052.33951
GTATCAA64250.041.9739041
ATCAACG74350.035.6016923
TCAACGC74450.035.553874
CAACGCA75700.034.9046825
AACGCAG80300.033.1251226
TATCAAC82850.032.0058822
ACGCAGA91300.029.1850727
CGCAGAG90850.029.0165568
GTACATG70750.026.8752691
CCGATCG1258.042043E-626.314559
TACATGG70450.025.6267452
GCAGAGT105800.024.9608069
ACATGGG75950.022.9043063
GACCGAT1452.5371259E-522.6870777
GAGTACT69100.021.79505312-13
CAGAGTA103200.020.78590810-11
AGAGTAC94550.020.74927310-11
GTGGTAT18750.019.8300461
CATGGGG48850.019.441514
Produced by FastQC (version 0.11.5)