##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2677367 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75780832437241 34.0 31.0 34.0 31.0 34.0 2 32.857208593368036 34.0 31.0 34.0 31.0 34.0 3 32.950739289757436 34.0 31.0 34.0 31.0 34.0 4 36.36662885588714 37.0 37.0 37.0 35.0 37.0 5 36.305206196983825 37.0 37.0 37.0 35.0 37.0 6 36.38712212408684 37.0 37.0 37.0 35.0 37.0 7 36.35814776233516 37.0 37.0 37.0 35.0 37.0 8 36.36020089886818 37.0 37.0 37.0 35.0 37.0 9 38.18277733310376 39.0 39.0 39.0 37.0 39.0 10-11 38.16203437929877 39.0 39.0 39.0 37.0 39.0 12-13 38.122077959428054 39.0 39.0 39.0 37.0 39.0 14-15 39.65608525092003 41.0 40.0 41.0 37.0 41.0 16-17 39.669062739624415 41.0 40.0 41.0 37.0 41.0 18-19 39.68151994104656 41.0 40.0 41.0 37.0 41.0 20-21 39.677747764874965 41.0 40.0 41.0 37.0 41.0 22-23 39.60888850874758 41.0 40.0 41.0 37.0 41.0 24-25 39.56075241085738 41.0 40.0 41.0 37.0 41.0 26-27 39.46050914947409 41.0 39.0 41.0 36.5 41.0 28-29 39.37311993462234 41.0 39.0 41.0 36.0 41.0 30-31 39.2870639325875 41.0 39.0 41.0 36.0 41.0 32-33 39.33149732554409 41.0 39.0 41.0 36.0 41.0 34-35 39.280221015647086 41.0 39.0 41.0 35.5 41.0 36-37 39.198858617440195 41.0 39.0 41.0 35.0 41.0 38-39 39.114183262884765 41.0 39.0 41.0 35.0 41.0 40-41 39.0084364601491 41.0 39.0 41.0 35.0 41.0 42-43 38.902184870434276 41.0 39.0 41.0 35.0 41.0 44-45 38.76843126101129 41.0 39.0 41.0 35.0 41.0 46-47 38.57696871590633 41.0 39.0 41.0 35.0 41.0 48-49 38.40916224783528 41.0 38.0 41.0 35.0 41.0 50-51 38.280642138339644 40.5 38.0 41.0 34.5 41.0 52-53 38.15025620320262 40.0 38.0 41.0 34.0 41.0 54-55 38.01461286405636 40.0 38.0 41.0 34.0 41.0 56-57 37.856702872635694 40.0 37.0 41.0 34.0 41.0 58-59 37.66586724942826 40.0 37.0 41.0 34.0 41.0 60-61 37.472292741338784 40.0 36.5 41.0 33.0 41.0 62-63 37.2347700184547 39.5 36.0 41.0 33.0 41.0 64-65 36.98231732892801 39.0 35.0 41.0 33.0 41.0 66-67 36.70163840071234 39.0 35.0 41.0 32.5 41.0 68-69 36.37926645842725 38.0 35.0 40.5 32.0 41.0 70-71 36.044465327315976 37.0 35.0 40.0 32.0 41.0 72-73 35.68851319225194 37.0 35.0 39.5 31.5 41.0 74-75 35.352830784871855 36.0 35.0 39.0 31.0 41.0 76-77 35.01201142764515 36.0 35.0 39.0 31.0 40.5 78-79 34.66954194176592 35.0 35.0 37.5 31.0 39.5 80-81 34.330188203559686 35.0 35.0 37.0 31.0 39.0 82-83 34.01028435772906 35.0 34.5 37.0 31.0 39.0 84-85 33.74208242650335 35.0 34.0 36.0 30.0 37.5 86-87 33.51329963355789 35.0 34.0 36.0 30.0 37.0 88-89 33.29759480116099 35.0 34.0 35.5 30.0 37.0 90-91 33.1038453077221 35.0 34.0 35.0 30.0 36.0 92-93 32.94290136540863 35.0 34.0 35.0 30.0 36.0 94-95 32.795023618353405 35.0 34.0 35.0 30.0 36.0 96-97 32.67504566986894 35.0 34.0 35.0 29.5 36.0 98-99 32.57054505415208 35.0 34.0 35.0 29.5 35.5 100 32.475463020198575 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1477606795478934 1101 2 0.18231917014746557 1101 3 0.14666663630222843 1101 4 0.08740889947237918 1101 5 0.03019796359767213 1101 6 0.11270625410516999 1101 7 0.1399298232845112 1101 8 0.15275068119165525 1101 9 0.21258682661562034 1101 10-11 0.20120858466425062 1101 12-13 0.18625918488357485 1101 14-15 0.23288065813790126 1101 16-17 0.2243275241272329 1101 18-19 0.2876305381799753 1101 20-21 0.27132327253548283 1101 22-23 0.2684920119877461 1101 24-25 0.25373787782481116 1101 26-27 0.2740756402441846 1101 28-29 0.28806026534452656 1101 30-31 0.24318174883836718 1101 32-33 0.24283932703855982 1101 34-35 0.2351279560493822 1101 36-37 0.24762684790711376 1101 38-39 0.2637006479628212 1101 40-41 0.34160999460771535 1101 42-43 0.2846336116856847 1101 44-45 0.22125164120675578 1101 46-47 0.22165163726625536 1101 48-49 0.15493039577697942 1101 50-51 0.1556125096997718 1101 52-53 0.2043728991278826 1101 54-55 0.20917272631546524 1101 56-57 0.20013491819910456 1101 58-59 0.209047796027086 1101 60-61 0.2012302748709871 1101 62-63 0.14459292473643615 1101 64-65 0.18430006083254113 1101 66-67 0.18084438337758968 1101 68-69 0.2009515900198906 1101 70-71 0.22860253082841098 1101 72-73 0.19953715631081792 1101 74-75 0.21902913335738816 1101 76-77 0.1774418398078268 1101 78-79 0.16744038601814992 1101 80-81 0.14149493703651217 1101 82-83 0.2086611174276385 1101 84-85 0.23979552997884923 1101 86-87 0.20426631384802363 1101 88-89 0.17025793048939164 1101 90-91 0.1584960188543647 1101 92-93 0.19761503475217523 1101 94-95 0.18618405272385985 1101 96-97 0.16365507225025766 1101 98-99 0.2393580716839665 1101 100 0.27886579227782704 1102 1 0.1397179410254452 1102 2 0.13689267944855033 1102 3 0.1390200088075062 1102 4 0.0844022137488949 1102 5 0.04962569043368603 1102 6 0.1014731731468288 1102 7 0.038806104822839416 1102 8 0.13325254421657462 1102 9 0.2021228576364038 1102 10-11 0.13326956790800182 1102 12-13 0.17061499510593592 1102 14-15 0.21835827898074456 1102 16-17 0.18843285999907522 1102 18-19 0.14261830099859907 1102 20-21 0.18731098171856786 1102 22-23 0.20878417806784455 1102 24-25 0.25544622362210134 1102 26-27 0.2799034831345466 1102 28-29 0.2690047835228313 1102 30-31 0.2605257531234457 1102 32-33 0.2328519223901182 1102 34-35 0.21745105147491728 1102 36-37 0.22994958336261107 1102 38-39 0.22617798334941597 1102 40-41 0.2685560556175943 1102 42-43 0.2767719380913931 1102 44-45 0.33578035583654753 1102 46-47 0.35324955505558364 1102 48-49 0.2793227573568089 1102 50-51 0.35215071385965757 1102 52-53 0.3666887879532936 1102 54-55 0.3195582938208261 1102 56-57 0.3860103419288379 1102 58-59 0.3147617443980195 1102 60-61 0.38850788988231955 1102 62-63 0.3279228203559299 1102 64-65 0.24775208708587115 1102 66-67 0.23310432925970304 1102 68-69 0.31222185588296014 1102 70-71 0.36859221408717957 1102 72-73 0.34334075809900355 1102 74-75 0.36710255572570816 1102 76-77 0.3425722983484576 1102 78-79 0.36507113523458656 1102 80-81 0.306369924842528 1102 82-83 0.2268833766067857 1102 84-85 0.26294721883226657 1102 86-87 0.2834286563746389 1102 88-89 0.25363976978508873 1102 90-91 0.25147141958200336 1102 92-93 0.2445983598601913 1102 94-95 0.24959097473166736 1102 96-97 0.31759942592226054 1102 98-99 0.34798327772182347 1102 100 0.3169008022435946 1103 1 0.14559598264635554 1103 2 0.1356555024311703 1103 3 0.14419386540840406 1103 4 0.05946958732253904 1103 5 0.028962941310020085 1103 6 0.09965264105155569 1103 7 0.13127921463390635 1103 8 0.15148294308180965 1103 9 0.13964417472560342 1103 10-11 0.12746575828983708 1103 12-13 0.14399852157028903 1103 14-15 0.20358227620794622 1103 16-17 0.17639611830109203 1103 18-19 0.13042010728533882 1103 20-21 0.14284687563803544 1103 22-23 0.1740877807414094 1103 24-25 0.16367121933709683 1103 26-27 0.1481919722552547 1103 28-29 0.1820909909541939 1103 30-31 0.21507749441832402 1103 32-33 0.2291177659485797 1103 34-35 0.16429002205355658 1103 36-37 0.09860491467465238 1103 38-39 0.07375394748979858 1103 40-41 0.16211599932425003 1103 42-43 0.13556805735697708 1103 44-45 0.17393365704666053 1103 46-47 0.11420333508112179 1103 48-49 0.041959935438100615 1103 50-51 0.021121854156483266 1103 52-53 0.017136159259564465 1103 54-55 9.855918125438734E-4 1103 56-57 -0.0018021311063662893 1103 58-59 0.07296769678908532 1103 60-61 0.06816412075220768 1103 62-63 -0.023878564787686685 1103 64-65 0.059070709287396994 1103 66-67 0.06428199839416493 1103 68-69 0.06584614965129276 1103 70-71 0.026276981134436994 1103 72-73 0.08191968606176658 1103 74-75 0.09459041418182323 1103 76-77 4.47878603338836E-4 1103 78-79 0.05452308889032764 1103 80-81 0.052128942407357215 1103 82-83 0.0557637224249774 1103 84-85 0.03539797821287749 1103 86-87 0.10909326078024151 1103 88-89 0.023636520710084596 1103 90-91 0.022827593712250405 1103 92-93 0.0019280706441549 1103 94-95 0.09204427437355633 1103 96-97 0.029442501195582338 1103 98-99 0.09540572773163092 1103 100 0.09926410741298497 1104 1 0.06233568232979536 1104 2 0.0824363048502974 1104 3 0.10070164775743962 1104 4 0.06593009031527686 1104 5 0.03307547688928736 1104 6 0.039630977639191656 1104 7 0.07379007212036015 1104 8 0.06497938470566567 1104 9 0.08649144035015865 1104 10-11 0.08911791914569989 1104 12-13 0.10818074172475889 1104 14-15 0.11262082639727566 1104 16-17 0.1371956673359378 1104 18-19 0.14818231577449126 1104 20-21 0.1470158284327283 1104 22-23 0.11382747516901048 1104 24-25 0.14563045084680226 1104 26-27 0.08356781502898514 1104 28-29 0.12856342805083187 1104 30-31 0.12965783848748913 1104 32-33 0.13447908461582614 1104 34-35 0.09694932268100587 1104 36-37 0.05507174797169512 1104 38-39 0.03871393351323604 1104 40-41 0.03857603594354231 1104 42-43 -0.0033716768820184484 1104 44-45 0.025277587635400778 1104 46-47 0.10174990007438112 1104 48-49 0.040758275361540086 1104 50-51 0.01225779815654704 1104 52-53 0.02484429437586755 1104 54-55 0.04013431590860961 1104 56-57 0.00607582115894445 1104 58-59 -0.01478951465166034 1104 60-61 6.45953311099845E-4 1104 62-63 -0.08208952166290828 1104 64-65 -0.04150295666001824 1104 66-67 -0.0853943377707509 1104 68-69 -0.03895092367904596 1104 70-71 -0.04356190979358843 1104 72-73 -0.009623200369375695 1104 74-75 -0.013159614708712297 1104 76-77 -0.06093514951485446 1104 78-79 -0.07695793020949537 1104 80-81 -0.06162097085449858 1104 82-83 -0.01671270658882662 1104 84-85 -0.005542325121467684 1104 86-87 -0.026669345955909307 1104 88-89 -0.05715186729638333 1104 90-91 -0.00397404315873473 1104 92-93 -9.173885060675957E-5 1104 94-95 -0.06579526029619132 1104 96-97 -0.0593102279373241 1104 98-99 -0.11571777121616833 1104 100 -0.12152925568708639 1105 1 0.03576131255109516 1105 2 0.01845661975997359 1105 3 0.02881726468978485 1105 4 0.012798921759362258 1105 5 0.03782125635725464 1105 6 -0.01802121603597584 1105 7 -0.04391716577420368 1105 8 -0.08832683092991545 1105 9 -1.4333011080225333E-5 1105 10-11 -0.032209588681922696 1105 12-13 0.013501620715075546 1105 14-15 0.03226963421126072 1105 16-17 -0.002921065902086184 1105 18-19 -0.05476001600553815 1105 20-21 -0.010812409943071088 1105 22-23 0.08013311675417611 1105 24-25 -0.04978759835625368 1105 26-27 -0.031608201030266514 1105 28-29 0.015170385922843366 1105 30-31 0.04552386069531167 1105 32-33 0.039975866708950036 1105 34-35 0.018826365189248406 1105 36-37 -0.004507559773330172 1105 38-39 -0.02923872879277667 1105 40-41 0.010749330042266081 1105 42-43 0.04557643479206064 1105 44-45 0.05610330305995603 1105 46-47 0.06475959508362905 1105 48-49 0.08890816153942893 1105 50-51 0.08134321079477758 1105 52-53 0.12115935285930846 1105 54-55 0.08631647518902952 1105 56-57 0.04278219035091979 1105 58-59 -0.01725128919536445 1105 60-61 0.062148481186078186 1105 62-63 0.06334124881649217 1105 64-65 -0.021041782877212256 1105 66-67 -0.02261427579853148 1105 68-69 -0.03483155363227297 1105 70-71 0.052916887253168454 1105 72-73 0.08766746705384776 1105 74-75 0.03375656833169671 1105 76-77 -0.03723328448499075 1105 78-79 0.013084312923858477 1105 80-81 0.04258642797884704 1105 82-83 0.08927979014300291 1105 84-85 0.13109393384367962 1105 86-87 0.08808429025987152 1105 88-89 0.05904278412938169 1105 90-91 0.040084856897856014 1105 92-93 0.06498240079946527 1105 94-95 0.029990511994057556 1105 96-97 0.06753705231944451 1105 98-99 -0.03287111064292958 1105 100 -0.03963680347489884 1106 1 0.0269895455560345 1106 2 0.06670374334896678 1106 3 0.021888371702466713 1106 4 0.012355763688901789 1106 5 -0.007309206702224458 1106 6 0.016246015680870585 1106 7 0.03731649600960907 1106 8 0.06022350380366959 1106 9 0.018070167106799317 1106 10-11 0.04863543099778411 1106 12-13 0.0773174929153484 1106 14-15 0.0748688050135442 1106 16-17 0.08563278276788111 1106 18-19 0.0881249442665748 1106 20-21 0.03443257364756391 1106 22-23 0.05605722559542414 1106 24-25 0.09824844237112274 1106 26-27 0.03220215173675456 1106 28-29 0.050527751958028944 1106 30-31 0.09856243820353683 1106 32-33 0.13354080573956395 1106 34-35 0.09979962689616428 1106 36-37 0.0925835570657938 1106 38-39 0.09812805197712038 1106 40-41 0.10825264678995694 1106 42-43 0.081841886352926 1106 44-45 0.10889510948288716 1106 46-47 0.0786599639482759 1106 48-49 0.013617510959818446 1106 50-51 0.011726321748405155 1106 52-53 0.00241158337328784 1106 54-55 0.01959105996268562 1106 56-57 0.07248189063027155 1106 58-59 0.08235083058767856 1106 60-61 0.10061134554498352 1106 62-63 0.04924103946655123 1106 64-65 0.0717020556622856 1106 66-67 0.12437171587988871 1106 68-69 0.07559916998762617 1106 70-71 0.05137138427462418 1106 72-73 0.09224884332668637 1106 74-75 0.06287805401758817 1106 76-77 0.02212402654087242 1106 78-79 0.012329568970990579 1106 80-81 0.11210356637410257 1106 82-83 0.06768187783316648 1106 84-85 0.11205099493481185 1106 86-87 0.08844339260390655 1106 88-89 0.048288430545355254 1106 90-91 0.05780458106433173 1106 92-93 0.04569691517598784 1106 94-95 -0.042514551546339874 1106 96-97 -0.04036355076651432 1106 98-99 -0.0025788575775962386 1106 100 0.09062817310636007 1107 1 0.002572869734947858 1107 2 0.00829649624748896 1107 3 0.019952673901549645 1107 4 0.037559222041601004 1107 5 -0.13525819182806487 1107 6 -0.1431361388911796 1107 7 -0.06030305052777152 1107 8 -0.06794166123837186 1107 9 -0.03464677275621142 1107 10-11 -0.02919062384007276 1107 12-13 -0.02083592896110531 1107 14-15 0.0624259086066985 1107 16-17 0.023476843235137324 1107 18-19 -0.0029303711355694873 1107 20-21 0.05736880254461596 1107 22-23 0.022789395713061822 1107 24-25 0.07816773679621747 1107 26-27 0.10902676820418122 1107 28-29 0.020328756462475894 1107 30-31 -0.022131490362298223 1107 32-33 -0.06374356928770197 1107 34-35 0.010157728897461027 1107 36-37 0.005572216615242098 1107 38-39 0.023975770708219102 1107 40-41 -0.004882216605487599 1107 42-43 -0.06943014448435036 1107 44-45 -0.05462120956065064 1107 46-47 -0.07692156002629247 1107 48-49 -0.09788542417413026 1107 50-51 -0.10172294984422336 1107 52-53 -0.07845031405881286 1107 54-55 -0.12353755650856613 1107 56-57 -0.09214180510315373 1107 58-59 -0.09880620592215905 1107 60-61 -0.11659633671344949 1107 62-63 -0.07235947606606885 1107 64-65 -0.13879577136199117 1107 66-67 -0.12459400337645121 1107 68-69 -0.09849391731140145 1107 70-71 -0.16013314068121787 1107 72-73 -0.1589227239161417 1107 74-75 -0.07083433429294672 1107 76-77 -0.12916055552184957 1107 78-79 -0.13794284641193855 1107 80-81 -0.10365361259812289 1107 82-83 -0.025288515499532593 1107 84-85 -0.05258704505626355 1107 86-87 -0.056233686479686185 1107 88-89 -0.07614540459393737 1107 90-91 -0.07694068344795824 1107 92-93 -0.04417532769333832 1107 94-95 -0.05612088678335425 1107 96-97 0.017101906169216363 1107 98-99 0.07406062831465476 1107 100 0.05604697701760841 1108 1 0.001908319536617853 1108 2 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-0.1923272277754009 2214 30-31 -0.1310988397819628 2214 32-33 -0.1746690659819663 2214 34-35 -0.15369870910883066 2214 36-37 -0.18748646123708568 2214 38-39 -0.21141309216825732 2214 40-41 -0.13666018567948157 2214 42-43 -0.09024604869664898 2214 44-45 -0.16115529480875068 2214 46-47 -0.14943488963495355 2214 48-49 -0.30029572418282413 2214 50-51 -0.24526921762380738 2214 52-53 -0.20460761038839337 2214 54-55 -0.20319578901554536 2214 56-57 -0.25575653775538854 2214 58-59 -0.3513639041892205 2214 60-61 -0.38568963250273924 2214 62-63 -0.3720896794658657 2214 64-65 -0.23690377530984819 2214 66-67 -0.3630792852545568 2214 68-69 -0.2727253476028437 2214 70-71 -0.27484338884820403 2214 72-73 -0.313788509047626 2214 74-75 -0.23376065369019727 2214 76-77 -0.2037877837161517 2214 78-79 -0.3289213664104196 2214 80-81 -0.2505213901830956 2214 82-83 -0.2816841479570229 2214 84-85 -0.2805993976647372 2214 86-87 -0.2672612562928407 2214 88-89 -0.3963703963028138 2214 90-91 -0.33076746946365176 2214 92-93 -0.26450642632926247 2214 94-95 -0.32833796037993324 2214 96-97 -0.3863738846806868 2214 98-99 -0.4535910727621797 2214 100 -0.6032438525800075 2215 1 -0.07669262104972319 2215 2 -0.14545518788890632 2215 3 -0.13990145973817647 2215 4 -0.0633719740507388 2215 5 -0.06056175833631272 2215 6 -0.12204724898021624 2215 7 -0.10199136007889109 2215 8 -0.11244147114668834 2215 9 -0.1802151258026825 2215 10-11 -0.14746220093437756 2215 12-13 -0.1678050214452469 2215 14-15 -0.2306590263000885 2215 16-17 -0.1886143699717806 2215 18-19 -0.2266289920530724 2215 20-21 -0.20089164121081637 2215 22-23 -0.1312436810844062 2215 24-25 -0.15627749928668777 2215 26-27 -0.15193298805650102 2215 28-29 -0.16550364258743855 2215 30-31 -0.1721962713306766 2215 32-33 -0.20580493113745746 2215 34-35 -0.16755732260873657 2215 36-37 -0.11123395611122078 2215 38-39 -0.25505799176957566 2215 40-41 -0.24727475150873346 2215 42-43 -0.21528542837922515 2215 44-45 -0.19468623948374386 2215 46-47 -0.16728795173163036 2215 48-49 -0.16379729041035063 2215 50-51 -0.12470088908154509 2215 52-53 -0.17424787421830246 2215 54-55 -0.19664727295759832 2215 56-57 -0.30386644305339416 2215 58-59 -0.23556256449963797 2215 60-61 -0.15444958339929116 2215 62-63 -0.16380196394218416 2215 64-65 -0.16228048111244675 2215 66-67 -0.1726359851571857 2215 68-69 -0.16093307636772636 2215 70-71 -0.1691673483237892 2215 72-73 -0.1319378797723374 2215 74-75 -0.14139901631237706 2215 76-77 -0.15219688307703905 2215 78-79 -0.1357001970106282 2215 80-81 -0.18152664485378978 2215 82-83 -0.10169445555882817 2215 84-85 -0.11049612510954887 2215 86-87 -0.1430631807767213 2215 88-89 -0.11874192412274454 2215 90-91 -0.13078684378577066 2215 92-93 -0.08277949983665422 2215 94-95 -0.09877278818547097 2215 96-97 -0.0696573322656775 2215 98-99 -0.12765444808701432 2215 100 -0.10114302479258441 2216 1 -0.09394419888278094 2216 2 -0.1065490906021509 2216 3 -0.13301439798770076 2216 4 -0.0904894162276264 2216 5 -0.051190484147277004 2216 6 -0.08577795045638226 2216 7 -0.113590505373935 2216 8 -0.11339705107995002 2216 9 -0.17749377224659924 2216 10-11 -0.2038066627615862 2216 12-13 -0.1837820465417721 2216 14-15 -0.32093045272467435 2216 16-17 -0.256775733379051 2216 18-19 -0.2035897032438143 2216 20-21 -0.18543666811511628 2216 22-23 -0.20879044191581642 2216 24-25 -0.3004579602617028 2216 26-27 -0.34368779724882614 2216 28-29 -0.3119423442393554 2216 30-31 -0.2794455802152527 2216 32-33 -0.28652706599459776 2216 34-35 -0.25529039752686344 2216 36-37 -0.31339938959484925 2216 38-39 -0.2284739555668267 2216 40-41 -0.33622196310211194 2216 42-43 -0.3076464935923795 2216 44-45 -0.21853258348587445 2216 46-47 -0.2800050757018795 2216 48-49 -0.2679832419083894 2216 50-51 -0.2424284383993367 2216 52-53 -0.19115800207327283 2216 54-55 -0.10035076366745699 2216 56-57 -0.12449992645609598 2216 58-59 -0.0656672001330989 2216 60-61 -0.08765923534739528 2216 62-63 -0.06826598624692082 2216 64-65 -0.07320472961787772 2216 66-67 -0.04514632650378303 2216 68-69 -0.12417311080996285 2216 70-71 -0.0908970255423256 2216 72-73 -0.0648916057771487 2216 74-75 -0.06130019612866988 2216 76-77 -0.049224006703902035 2216 78-79 -0.0315869847888095 2216 80-81 -0.04839106142755867 2216 82-83 -0.09346979421184898 2216 84-85 -0.021020076823965894 2216 86-87 -0.07168748906128997 2216 88-89 -0.06773798364784511 2216 90-91 -0.09979686592878068 2216 92-93 -0.09768435907876949 2216 94-95 -0.11487618392812493 2216 96-97 -0.18666888894896516 2216 98-99 -0.12137999319774195 2216 100 -0.1280622801344009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20.0 3 7.0 4 9.0 5 24.0 6 54.0 7 126.0 8 245.0 9 509.0 10 940.0 11 1593.0 12 2480.0 13 3662.0 14 4471.0 15 5875.0 16 7268.0 17 10370.0 18 12142.0 19 6816.0 20 4699.0 21 4686.0 22 4704.0 23 5121.0 24 5810.0 25 7183.0 26 9023.0 27 11783.0 28 14885.0 29 19544.0 30 24985.0 31 32119.0 32 41383.0 33 54024.0 34 75875.0 35 116475.0 36 221683.0 37 521595.0 38 962868.0 39 480551.0 40 1760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7365803288568 19.763410324796755 13.57665237797223 23.923356968374208 2 15.561453643876897 20.064041486153915 40.50480199094096 23.869702879028225 3 18.07992703279005 25.462329221208748 30.11133699638488 26.346406749616317 4 12.134323162633097 16.732017013749346 38.9200721306479 32.213587692969654 5 12.513385807942335 37.95662138844737 35.98508710770148 13.544905695908819 6 31.03882182448382 38.283682433441896 17.480914034720772 13.196581707353513 7 27.302612043015735 32.54585950599365 22.567049135811967 17.584479315178655 8 25.0805550689653 36.54643509037444 20.155145309130894 18.21786453152937 9 25.13630015802863 16.298649081204157 21.15693688155099 37.40811387921623 10-11 23.972395906185824 26.552328761597494 28.984952134689575 20.490323197527115 12-13 24.889602359332883 23.918741808650065 29.17121560099904 22.020440231018014 14-15 21.93626942908419 25.81917295549916 26.57080749169426 25.67375012372239 16-17 21.194180700665992 29.288793803763173 27.109208412593418 22.40781708297742 18-19 21.01008286320372 27.907313329955635 29.694892122458484 21.38771168438216 20-21 22.158523654022776 27.181686335866544 29.4452721647798 21.214517845330878 22-23 21.748505337905495 26.999739669024837 29.45631911423226 21.795435878837406 24-25 21.68166399364151 27.13687631799313 29.350544527718657 21.830915160646697 26-27 21.38396939037727 27.152332474765885 29.515808298422908 21.947889836433937 28-29 21.4355017975336 27.196518075909726 29.550074043555053 21.81790608300162 30-31 21.665433965351973 27.56837008614088 29.1974787506144 21.568717197892745 32-33 21.396660609357347 27.25206376912087 29.61755264778141 21.73372297374037 34-35 21.602652200886247 27.08775169111058 29.555051718253534 21.75454438974964 36-37 21.866353490061112 27.430773748457966 29.029334341555774 21.673538419925144 38-39 21.627325514604312 27.6379711154718 29.125366473735774 21.609336896188115 40-41 21.523676017465917 27.384479962279478 29.24588468563926 21.84595933461534 42-43 21.617340496275318 27.245698125331558 29.172089930288337 21.964871448104784 44-45 21.674211204469863 27.510627543409576 28.97255686048601 21.84260439163455 46-47 21.81942645387415 27.74910182045375 28.638773526140366 21.792698199531742 48-49 21.80942779254912 27.68920991548336 28.43720046946217 22.064161822505348 50-51 21.778679638014967 27.676316425944066 28.394851677089626 22.15015225895134 52-53 21.825491613625207 27.70233183221685 28.385220617059538 22.086955937098406 54-55 21.912904173449853 28.066225682547085 28.171650257846657 21.849219886156398 56-57 21.721430952552147 27.72951930250821 28.337536872398854 22.21151287254079 58-59 21.623144408092116 27.375500332138557 28.71512895323784 22.286226306531486 60-61 21.959456178684146 27.755411134143838 28.268997329448897 22.016135357723122 62-63 21.692857176205838 27.77529915447716 28.357127183255752 22.174716486061246 64-65 21.745344542686478 27.48792808244761 28.45612799220704 22.310599382658868 66-67 21.59530178192623 27.552448675568115 28.364649371366635 22.48760017113902 68-69 22.00090836291489 27.69395714558906 28.293357147979492 22.01177734351656 70-71 21.997151669668384 27.464388400804076 28.297018926867455 22.241441002660086 72-73 21.802103868222133 27.649556240975233 28.288171829350915 22.260168061451715 74-75 21.869521176504865 27.679268412021717 28.188670605381283 22.262539806092136 76-77 21.882486391435137 27.70272036836314 28.062160532883535 22.352632707318186 78-79 21.98778575659809 27.767944315420458 28.13619803941635 22.108071888565103 80-81 21.885958269471857 27.67617316337139 28.193476782685234 22.244391784471517 82-83 21.803030461486518 27.70062016592539 28.261454992029428 22.23489438055867 84-85 21.74693989144514 27.5994862095291 28.190778514325327 22.462795384700428 86-87 21.899130562098065 27.76313027524855 28.0306137160384 22.30712544661499 88-89 21.651327591622664 27.64944066315899 28.16377059999619 22.53546114522215 90-91 21.610373176333315 27.524952686725427 28.25361633276275 22.611057804178508 92-93 21.73284424585796 27.7205553067622 28.057995037662003 22.48860540971783 94-95 21.485119522276925 27.47538906694525 28.285513342025954 22.753978068751874 96-97 21.499312570895214 27.444705936840187 28.237742528387034 22.818238963877572 98-99 21.46444622646055 27.57897964679478 28.10522427444575 22.85134985229892 100 21.619748058446973 27.410511894708495 28.052187092766882 22.917552954077642 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10.0 1 13.5 2 71.0 3 133.5 4 100.5 5 70.5 6 174.5 7 473.0 8 809.0 9 965.5 10 861.5 11 734.5 12 782.0 13 901.0 14 1062.5 15 1240.0 16 1449.0 17 1649.5 18 1947.5 19 2322.0 20 2794.5 21 3560.5 22 4672.0 23 6519.5 24 8792.0 25 11603.0 26 15492.0 27 20737.0 28 27455.0 29 34622.0 30 41802.0 31 49403.0 32 57268.0 33 64675.5 34 71869.0 35 78620.5 36 84163.0 37 89273.5 38 92841.5 39 94595.0 40 96407.5 41 97307.5 42 96065.0 43 95290.0 44 94588.0 45 93382.5 46 93172.5 47 90948.0 48 87902.0 49 86094.0 50 84265.0 51 82230.5 52 76678.5 53 71344.5 54 68667.0 55 65066.5 56 60351.5 57 54594.0 58 49042.5 59 44530.0 60 40078.5 61 34605.5 62 29675.5 63 24926.5 64 20214.5 65 16417.0 66 12778.5 67 9322.5 68 6855.0 69 5213.5 70 3727.0 71 2555.5 72 1890.5 73 1453.5 74 1123.5 75 801.5 76 503.0 77 347.0 78 203.5 79 98.5 80 58.5 81 28.0 82 17.0 83 8.5 84 4.5 85 4.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09430907305572975 2 0.0014940051177145307 3 0.0 4 1.120503838285898E-4 5 0.03828388114143485 6 2.6145089560004284E-4 7 0.004257914585486413 8 2.241007676571796E-4 9 1.4940051177145307E-4 10-11 3.9217634340006435E-4 12-13 0.0 14-15 7.470025588572653E-5 16-17 0.0 18-19 9.337531985715817E-5 20-21 0.0 22-23 2.241007676571796E-4 24-25 3.735012794286327E-5 26-27 0.0014566549897716673 28-29 0.00845980397905853 30-31 0.03718205236712038 32-33 0.02655594096737578 34-35 0.02978672703443346 36-37 0.026481240711490057 38-39 0.025192661297461273 40-41 0.03137410747200515 42-43 0.02341853022017527 44-45 0.02226067625394651 46-47 0.015855129311745455 48-49 0.009748383393087312 50-51 0.006891098605458273 52-53 0.0051356425921437 54-55 0.0036416374744291688 56-57 0.0022223326126003646 58-59 7.65677622828697E-4 60-61 6.349521750286755E-4 62-63 0.0011018287743144665 64-65 0.0029506601074861982 66-67 4.295264713429276E-4 68-69 8.590529426858552E-4 70-71 3.3615115148576943E-4 72-73 4.8555166325722245E-4 74-75 4.8555166325722245E-4 76-77 3.3615115148576943E-4 78-79 3.735012794286327E-5 80-81 8.403778787144235E-4 82-83 7.096524309144021E-4 84-85 4.1085140737149597E-4 86-87 1.8675063971431634E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2677367.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.65241025215561 #Duplication Level Percentage of deduplicated Percentage of total 1 75.45672453306729 46.52088937196559 2 14.656747302711215 18.07247595337854 3 4.69260590168944 8.679313926079317 4 1.9380027867332184 4.779301715099889 5 0.9838805901302097 3.032930489092032 6 0.5681698472212181 2.1017424308272266 7 0.3606625611528857 1.556500132795361 8 0.2594048319066736 1.2794346494481368 9 0.17521701083404922 0.9722295931587478 >10 0.8472435654555682 9.168864333699908 >50 0.04553890761305136 1.9128857681034777 >100 0.01543808374968942 1.492762537657993 >500 1.2135924520800195E-4 0.04732421907771085 >1k 2.4271849030586953E-4 0.383344879616058 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3942 0.147234204350767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3488 0.13027724626470708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.735012794286327E-5 0.0 16-17 0.0 0.0 0.0 3.735012794286327E-5 0.0 18-19 0.0 0.0 0.0 3.735012794286327E-5 0.0 20-21 0.0 0.0 0.0 3.735012794286327E-5 0.0 22-23 0.0 0.0 0.0 9.337531985715817E-5 0.0 24-25 0.0 0.0 0.0 2.4277583162861125E-4 0.0 26-27 0.0 0.0 0.0 4.295264713429276E-4 0.0 28-29 0.0 0.0 0.0 7.843526868001286E-4 0.0 30-31 0.0 0.0 0.0 0.003062710491314788 0.0 32-33 0.0 0.0 0.0 0.00948693249748727 0.0 34-35 0.0 0.0 0.0 0.018394938011860158 0.0 36-37 0.0 0.0 0.0 0.03466091873097711 0.0 38-39 0.0 0.0 0.0 0.06469042159703918 0.0 40-41 0.0 0.0 0.0 0.09951941590375918 0.0 42-43 0.0 0.0 0.0 0.13485263693770783 0.0 44-45 0.0 0.0 0.0 0.1771516568330005 0.0 46-47 0.0 0.0 0.0 0.22852675781840892 0.0 48-49 0.0 0.0 0.0 0.2888285393821617 0.0 50-51 0.0 0.0 0.0 0.3559280442315155 0.0 52-53 0.0 0.0 0.0 0.42302754908086937 0.0 54-55 0.0 0.0 0.0 0.49318976442153806 0.0 56-57 0.0 0.0 0.0 0.5804396632960667 0.0 58-59 0.0 0.0 0.0 0.6796976283042258 0.0 60-61 0.0 0.0 0.0 0.7869111705642147 0.0 62-63 0.0 0.0 0.0 0.895133166278661 0.0 64-65 0.0 0.0 0.0 1.0097607089353084 0.0 66-67 0.0 0.0 0.0 1.1370125948366436 0.0 68-69 0.0 0.0 0.0 1.2843775246352107 0.0 70-71 0.0 0.0 0.0 1.4488301379676376 0.0 72-73 0.0 0.0 0.0 1.6189412956834084 0.0 74-75 0.0 0.0 0.0 1.7884735264160647 0.0 76-77 0.0 0.0 0.0 1.9697336973227801 0.0 78-79 0.0 0.0 0.0 2.1815275978227864 0.0 80-81 0.0 0.0 0.0 2.416198451687796 0.0 82-83 0.0 0.0 0.0 2.660711064265751 0.0 84-85 0.0 0.0 0.0 2.9083424125269337 0.0 86-87 0.0 0.0 0.0 3.16200580645089 0.0 88 0.0 0.0 0.0 3.3703634951801527 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3480 0.0 52.3395 1 GTATCAA 6425 0.0 41.973904 1 ATCAACG 7435 0.0 35.601692 3 TCAACGC 7445 0.0 35.55387 4 CAACGCA 7570 0.0 34.904682 5 AACGCAG 8030 0.0 33.125122 6 TATCAAC 8285 0.0 32.005882 2 ACGCAGA 9130 0.0 29.185072 7 CGCAGAG 9085 0.0 29.016556 8 GTACATG 7075 0.0 26.875269 1 CCGATCG 125 8.042043E-6 26.31455 9 TACATGG 7045 0.0 25.626745 2 GCAGAGT 10580 0.0 24.960806 9 ACATGGG 7595 0.0 22.904306 3 GACCGAT 145 2.5371259E-5 22.687077 7 GAGTACT 6910 0.0 21.795053 12-13 CAGAGTA 10320 0.0 20.785908 10-11 AGAGTAC 9455 0.0 20.749273 10-11 GTGGTAT 1875 0.0 19.830046 1 CATGGGG 4885 0.0 19.44151 4 >>END_MODULE