FastQCFastQC Report
Wed 25 May 2016
SRR1033190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666888
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG38300.032.7524833
TCAACGC39000.032.1589134
GGTATCA26400.031.9559781
GTATCAA42900.031.6401231
CAACGCA39650.031.5123885
AACGCAG43550.028.7884226
GTACATG88800.028.3951241
TACATGG89550.026.2466142
TATCAAC52600.024.8443682
ACATGGG96550.023.8438053
ACGCAGA52050.023.5458587
CGCAGAG52650.023.0099728
GTATAGG13400.021.8066961
CATGGGG66850.020.3775184
GAGTACA68400.019.7755581
GTCGTAC3004.101821E-918.8522421
GTACCGT1550.001040374318.176676
TACCGTC3157.863491E-917.8881517
TATACTG23500.017.589675
AGTACAT69600.017.2901842
Produced by FastQC (version 0.11.5)