##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033190_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3666888
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.667533887045362	33.0	31.0	34.0	30.0	34.0
2	31.82287678271057	34.0	31.0	34.0	30.0	34.0
3	31.943083890208808	34.0	31.0	34.0	30.0	34.0
4	35.32921130942641	37.0	35.0	37.0	33.0	37.0
5	35.416992283374896	37.0	35.0	37.0	33.0	37.0
6	35.5613752042604	37.0	37.0	37.0	35.0	37.0
7	35.520471309731846	37.0	37.0	37.0	35.0	37.0
8	35.53749500939216	37.0	37.0	37.0	35.0	37.0
9	37.23397987612384	39.0	38.0	39.0	35.0	39.0
10-11	37.21422538675847	39.0	38.0	39.0	35.0	39.0
12-13	37.168611640170084	39.0	38.0	39.0	35.0	39.0
14-15	38.65085598469329	41.0	39.0	41.0	35.5	41.0
16-17	38.66305311206669	41.0	39.0	41.0	35.5	41.0
18-19	38.65394143480793	41.0	39.0	41.0	35.0	41.0
20-21	38.6780522066668	41.0	39.0	41.0	35.0	41.0
22-23	38.64914731510753	41.0	39.0	41.0	35.0	41.0
24-25	38.64006331799608	41.0	39.0	41.0	34.5	41.0
26-27	38.59221334275822	41.0	39.0	41.0	34.0	41.0
28-29	38.56309914565157	40.5	39.0	41.0	34.0	41.0
30-31	38.56322595617864	41.0	39.0	41.0	34.0	41.0
32-33	38.62387970398878	41.0	39.0	41.0	35.0	41.0
34-35	38.59933409474192	41.0	39.0	41.0	34.5	41.0
36-37	38.52815861842521	41.0	39.0	41.0	34.0	41.0
38-39	38.453711157799205	41.0	39.0	41.0	34.0	41.0
40-41	38.3954927175305	41.0	39.0	41.0	34.0	41.0
42-43	38.29242616627506	41.0	38.5	41.0	34.0	41.0
44-45	38.1841981538569	40.0	38.0	41.0	33.0	41.0
46-47	38.06805416473042	40.0	38.0	41.0	33.0	41.0
48-49	37.95224479176893	40.0	38.0	41.0	33.0	41.0
50-51	37.836544639487215	40.0	38.0	41.0	33.0	41.0
52-53	37.69272650269111	40.0	37.5	41.0	33.0	41.0
54-55	37.54341351576596	40.0	37.0	41.0	32.0	41.0
56-57	37.390341892089424	40.0	37.0	41.0	32.0	41.0
58-59	37.19704392389404	40.0	36.5	41.0	32.0	41.0
60-61	36.978120547996014	39.5	36.0	41.0	31.0	41.0
62-63	36.71886269774261	39.0	35.0	41.0	31.0	41.0
64-65	36.449820256304534	39.0	35.0	41.0	31.0	41.0
66-67	36.15926106824097	38.5	35.0	41.0	31.0	41.0
68-69	35.849364911063546	37.5	35.0	40.0	30.5	41.0
70-71	35.49734366034632	37.0	35.0	40.0	30.0	41.0
72-73	35.132428506133806	36.5	35.0	39.0	30.0	41.0
74-75	34.760161204814544	36.0	34.5	39.0	29.5	41.0
76-77	34.420854550234424	36.0	34.0	39.0	29.0	40.0
78-79	34.05030314533741	35.0	34.0	37.5	29.0	39.5
80-81	33.681472272946436	35.0	34.0	37.0	29.0	39.0
82-83	33.45551404897013	35.0	34.0	37.0	29.0	39.0
84-85	33.18469462388816	35.0	34.0	36.0	29.0	37.5
86-87	32.93439109675562	35.0	34.0	36.0	29.0	37.0
88-89	32.572621525391554	35.0	34.0	35.5	28.0	37.0
90-91	32.387123631809864	35.0	34.0	35.0	27.5	36.0
92-93	32.21632226018357	35.0	34.0	35.0	27.0	36.0
94-95	32.058026042791596	35.0	34.0	35.0	27.0	36.0
96-97	31.89592946389418	35.0	34.0	35.0	27.0	36.0
98-99	31.71446155432072	35.0	33.5	35.0	26.5	35.5
100	31.559153156573093	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20351714526339748
1101	2	0.20522915433550892
1101	3	0.19289037765176076
1101	4	0.20283595521993902
1101	5	0.09576750860618688
1101	6	0.21706977096277313
1101	7	0.23167448758280074
1101	8	0.18699929911157653
1101	9	0.2769621616166731
1101	10-11	0.24974832514291023
1101	12-13	0.2845239444306529
1101	14-15	0.284600509774215
1101	16-17	0.22235022843609187
1101	18-19	0.22568637176006234
1101	20-21	0.28216496347764064
1101	22-23	0.2809595664883844
1101	24-25	0.2744209933811703
1101	26-27	0.2616221054646033
1101	28-29	0.37646100001023086
1101	30-31	0.36417429970558146
1101	32-33	0.30164644108829464
1101	34-35	0.29165883078438526
1101	36-37	0.26994776078733196
1101	38-39	0.2566218529816666
1101	40-41	0.2562487529737254
1101	42-43	0.2722367227066158
1101	44-45	0.22652565536614588
1101	46-47	0.2589779355134709
1101	48-49	0.23373919463729464
1101	50-51	0.19344081135503188
1101	52-53	0.16801831466757733
1101	54-55	0.14750321058739502
1101	56-57	0.2406719187419739
1101	58-59	0.24787530489954435
1101	60-61	0.209534868238606
1101	62-63	0.2184005437518053
1101	64-65	0.22351664809865213
1101	66-67	0.1799196329589634
1101	68-69	0.21849530635013537
1101	70-71	0.26098910143374354
1101	72-73	0.26436318817020776
1101	74-75	0.19359462971748087
1101	76-77	0.16299678427990472
1101	78-79	0.2334711532802487
1101	80-81	0.23201703921557737
1101	82-83	0.20227111697403188
1101	84-85	0.1168007358234675
1101	86-87	0.19959234024180716
1101	88-89	0.37022065195708365
1101	90-91	0.39815617996176655
1101	92-93	0.34510693839345663
1101	94-95	0.3729698684196663
1101	96-97	0.39423105189278473
1101	98-99	0.48052685246170057
1101	100	0.4577490779930784
1102	1	0.024181595113752508
1102	2	0.07091028215516815
1102	3	0.06987485977804297
1102	4	0.019849900774886464
1102	5	-0.07220777523196631
1102	6	-0.006638956666662921
1102	7	0.002297252480460088
1102	8	0.03767914830553565
1102	9	0.06356615249224973
1102	10-11	0.027090276078460818
1102	12-13	0.06871126891874013
1102	14-15	0.12057668187037507
1102	16-17	0.1377657482879684
1102	18-19	0.10848945829710033
1102	20-21	0.13174846890299108
1102	22-23	0.08312358664711894
1102	24-25	0.1316711680256475
1102	26-27	0.170978112030177
1102	28-29	0.1363642272678831
1102	30-31	0.21571510748754008
1102	32-33	0.18798371526887792
1102	34-35	0.20091451348994838
1102	36-37	0.18849866548480776
1102	38-39	0.12995967945198572
1102	40-41	0.18200259575046118
1102	42-43	0.14937391405968725
1102	44-45	0.11546191774938563
1102	46-47	0.14210508546715062
1102	48-49	0.15906404373473748
1102	50-51	0.13358449761618374
1102	52-53	0.11709421446353474
1102	54-55	0.16121371935917495
1102	56-57	0.11269349058534317
1102	58-59	0.05404360506438621
1102	60-61	0.06705434793765619
1102	62-63	0.09372263299589179
1102	64-65	0.18362522120820302
1102	66-67	0.08766658918369785
1102	68-69	0.06841232729104263
1102	70-71	0.12328079432300143
1102	72-73	0.12365431824081696
1102	74-75	0.11675678274767876
1102	76-77	0.005874424346849594
1102	78-79	0.05548791133315234
1102	80-81	0.03518236368419281
1102	82-83	0.056655949649552895
1102	84-85	0.054154278143109025
1102	86-87	0.07358128471594938
1102	88-89	0.2656738972494992
1102	90-91	0.2778582801144509
1102	92-93	0.32355146669703316
1102	94-95	0.2715292449097646
1102	96-97	0.22872771127375913
1102	98-99	0.20433209519399398
1102	100	0.20069155068448907
1103	1	0.005532029778347436
1103	2	0.011780770783552441
1103	3	0.001761352302448671
1103	4	0.027527762739914863
1103	5	-0.04921749550979371
1103	6	0.08238075935157241
1103	7	0.13974305977682633
1103	8	0.12056581954468726
1103	9	0.16510929962631593
1103	10-11	0.13168973333080203
1103	12-13	0.13401943476218747
1103	14-15	0.15381031118681676
1103	16-17	0.1592617538885932
1103	18-19	0.12783616471484294
1103	20-21	0.16775670705776236
1103	22-23	0.14642849646527623
1103	24-25	0.16826018111905228
1103	26-27	0.21763097293462863
1103	28-29	0.22379080977161436
1103	30-31	0.24368377347505543
1103	32-33	0.17620756518381597
1103	34-35	0.14163264347718751
1103	36-37	0.17746088948713634
1103	38-39	0.2412470610198696
1103	40-41	0.2111123102886907
1103	42-43	0.15756252396032977
1103	44-45	0.08821327447458316
1103	46-47	0.18414399614030685
1103	48-49	0.17933250361481612
1103	50-51	0.14158421889028716
1103	52-53	0.07903538295083479
1103	54-55	0.14603047147683412
1103	56-57	0.13596042303980482
1103	58-59	0.1071660357999491
1103	60-61	0.0794143443211226
1103	62-63	0.13994074292331504
1103	64-65	0.14825169874821142
1103	66-67	0.16430179552820334
1103	68-69	0.1349464647831553
1103	70-71	0.11375117115389344
1103	72-73	0.16607350164687773
1103	74-75	0.2550150798821633
1103	76-77	0.1852142125266809
1103	78-79	0.12711086967073726
1103	80-81	0.16725423196739797
1103	82-83	0.14900605625452812
1103	84-85	0.15357933878807017
1103	86-87	0.19329062274385223
1103	88-89	0.1939018435905453
1103	90-91	0.2543801532830301
1103	92-93	0.28449060405572624
1103	94-95	0.18696014037046638
1103	96-97	0.18382587097256575
1103	98-99	0.2686850105574905
1103	100	0.3369745411676881
1104	1	0.03752835433081714
1104	2	0.04675640787413471
1104	3	0.011807986993495945
1104	4	0.016210767915637803
1104	5	0.027394746772777978
1104	6	0.04914144810953047
1104	7	0.04540527837990993
1104	8	0.0059401777073233575
1104	9	0.057947568136199834
1104	10-11	0.10559512686126027
1104	12-13	0.09023951434484445
1104	14-15	0.010138414219873937
1104	16-17	0.07556686903767229
1104	18-19	0.06263126051191392
1104	20-21	0.07577612768103847
1104	22-23	0.10044269035820008
1104	24-25	0.17614810756072785
1104	26-27	0.1539191376051363
1104	28-29	0.24832395006287555
1104	30-31	0.2196664810099591
1104	32-33	0.15983712044340592
1104	34-35	0.1718081620705263
1104	36-37	0.2026866432506722
1104	38-39	0.1679766115780552
1104	40-41	0.16836032802842027
1104	42-43	0.18474647731365934
1104	44-45	0.13930606119097888
1104	46-47	0.09048093019480774
1104	48-49	0.09269121607547959
1104	50-51	0.13181602004290482
1104	52-53	0.058623724840799696
1104	54-55	0.0694740289063347
1104	56-57	0.09197863255157301
1104	58-59	0.039009519241275825
1104	60-61	0.07640137769512734
1104	62-63	0.061527171939602
1104	64-65	0.008413608673976114
1104	66-67	0.09733580594282643
1104	68-69	0.016457990763683483
1104	70-71	0.04242797555484401
1104	72-73	0.07993422999467725
1104	74-75	0.021106117638815647
1104	76-77	-0.030149187182409776
1104	78-79	0.020597587469588063
1104	80-81	0.03196830200820244
1104	82-83	0.014880193959285748
1104	84-85	0.044298888237157996
1104	86-87	0.13323557453083623
1104	88-89	0.2085861531798301
1104	90-91	0.13578478124652804
1104	92-93	0.11931844633313915
1104	94-95	0.16939555637873838
1104	96-97	0.14640357806183246
1104	98-99	0.1661227618399863
1104	100	0.2169704533032899
1105	1	0.08552909910349626
1105	2	0.1139069431430606
1105	3	0.1075684594397508
1105	4	0.15952159614144534
1105	5	0.09914809793752966
1105	6	0.10535162529075137
1105	7	0.07843187298248466
1105	8	0.1428450032263484
1105	9	0.18699900450233997
1105	10-11	0.17237350133312646
1105	12-13	0.190248928099372
1105	14-15	0.216062663787838
1105	16-17	0.22459760277755692
1105	18-19	0.22122727796285346
1105	20-21	0.2304954513674815
1105	22-23	0.31530757782410745
1105	24-25	0.3029227200006517
1105	26-27	0.2869741434948949
1105	28-29	0.29454706360962035
1105	30-31	0.3220129556161382
1105	32-33	0.3239502469161124
1105	34-35	0.31649465455069503
1105	36-37	0.2758783071541657
1105	38-39	0.24820171724597628
1105	40-41	0.19441819392297788
1105	42-43	0.2821955267985814
1105	44-45	0.2561821090046692
1105	46-47	0.31042421379844143
1105	48-49	0.3143654611673199
1105	50-51	0.34758581463916016
1105	52-53	0.31583260318066664
1105	54-55	0.29526643594473256
1105	56-57	0.3236888490811083
1105	58-59	0.26446033502828925
1105	60-61	0.29539160973637024
1105	62-63	0.3375981018908547
1105	64-65	0.3928695394642432
1105	66-67	0.428688757822826
1105	68-69	0.3306652790708
1105	70-71	0.3504507081932502
1105	72-73	0.3374286091539176
1105	74-75	0.322411340083093
1105	76-77	0.3327940590854368
1105	78-79	0.34406992907458545
1105	80-81	0.36867559135627204
1105	82-83	0.36285714931025836
1105	84-85	0.32875883366583736
1105	86-87	0.3538568878817969
1105	88-89	0.30411664271033345
1105	90-91	0.32933882646835855
1105	92-93	0.3335720990295101
1105	94-95	0.3978168575990466
1105	96-97	0.414993956281716
1105	98-99	0.44137674583944886
1105	100	0.4550818841204176
1106	1	-0.004893711131771283
1106	2	-0.03628789948878719
1106	3	-0.040029310354160685
1106	4	-0.09026460879862697
1106	5	-0.07968822032585621
1106	6	-0.04520170868537576
1106	7	-0.043116951535935755
1106	8	-0.05288427466757639
1106	9	-0.10133606116338001
1106	10-11	-0.11175487795840411
1106	12-13	-0.11846452365502103
1106	14-15	-0.03287338416804175
1106	16-17	-0.07643956851751454
1106	18-19	-0.018526607052805844
1106	20-21	-0.07464469182480826
1106	22-23	0.018932998932072564
1106	24-25	0.04673347238021108
1106	26-27	0.07418244155491038
1106	28-29	0.07898771366331658
1106	30-31	0.10323378177488962
1106	32-33	0.12273714894477905
1106	34-35	0.0660927941092666
1106	36-37	0.04168990741946743
1106	38-39	0.05539909080284389
1106	40-41	0.014831487492905637
1106	42-43	0.07632640147103587
1106	44-45	0.06114308941682367
1106	46-47	0.014527126013419434
1106	48-49	0.029849860162485697
1106	50-51	0.048420527184930506
1106	52-53	-0.0015823539323349678
1106	54-55	-0.0077593974481757755
1106	56-57	-0.02039792041320254
1106	58-59	-0.0319786177950121
1106	60-61	-0.0974872508261484
1106	62-63	-0.015786067009365468
1106	64-65	-0.06507702529874848
1106	66-67	0.022995489542253722
1106	68-69	-0.008382087543033379
1106	70-71	-0.09321432667245233
1106	72-73	-0.07310788978752214
1106	74-75	0.00924330512362559
1106	76-77	-0.0031520689361670406
1106	78-79	0.019873411228466864
1106	80-81	0.010575082876819408
1106	82-83	-0.05551222966681735
1106	84-85	-0.0027522405071280787
1106	86-87	0.023601577853092692
1106	88-89	0.1436820843569464
1106	90-91	0.13406638299374407
1106	92-93	0.1261866468284225
1106	94-95	0.13512662210484194
1106	96-97	0.14746269505399923
1106	98-99	0.11252463167554083
1106	100	0.1387675836314628
1107	1	0.028216329953092867
1107	2	0.07885347762107742
1107	3	0.030663202227490416
1107	4	0.005146708047099935
1107	5	-0.0697939067841915
1107	6	-0.03370349412757889
1107	7	-0.05672200767486402
1107	8	-0.08026718299942814
1107	9	-0.06985239032664481
1107	10-11	-0.073417692407169
1107	12-13	-0.07066683393890116
1107	14-15	-0.07396241230800626
1107	16-17	-0.12130577349678617
1107	18-19	-0.028845633762273337
1107	20-21	-0.04423569818276718
1107	22-23	-0.051880253855024705
1107	24-25	-0.024708424196589363
1107	26-27	0.003302770484040707
1107	28-29	-0.011707793326500848
1107	30-31	-0.039191961402934794
1107	32-33	-0.03103825333585064
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2216	14-15	-0.20331570024718815
2216	16-17	-0.15654726545422903
2216	18-19	-0.1934424705113713
2216	20-21	-0.20151674085909832
2216	22-23	-0.15813553210146125
2216	24-25	-0.24018293199716823
2216	26-27	-0.27790165779835263
2216	28-29	-0.19440196087259665
2216	30-31	-0.2268202357305924
2216	32-33	-0.1894233687145288
2216	34-35	-0.19654164281114106
2216	36-37	-0.1938632260218185
2216	38-39	-0.23051476111386648
2216	40-41	-0.22290263062347293
2216	42-43	-0.2345909765602201
2216	44-45	-0.1997493184494914
2216	46-47	-0.11137935588439518
2216	48-49	-0.12909037168884652
2216	50-51	-0.08699705200051255
2216	52-53	-0.07959969714194415
2216	54-55	-0.11992302539068334
2216	56-57	-0.16830025752057054
2216	58-59	-0.2245867998682911
2216	60-61	-0.15207846833298788
2216	62-63	-0.1292633703938293
2216	64-65	-0.08157627984500238
2216	66-67	-0.1362114558553742
2216	68-69	-0.1096632267180695
2216	70-71	-0.1716982799776403
2216	72-73	-0.10119143469898972
2216	74-75	-0.07252785240884663
2216	76-77	-0.12641040162151995
2216	78-79	-0.08289954147831935
2216	80-81	-0.08837074748428364
2216	82-83	-0.051563671046423565
2216	84-85	-0.11150714680984919
2216	86-87	-0.13614640806948586
2216	88-89	-0.1435566670671875
2216	90-91	-0.1411067414547702
2216	92-93	-0.07371977421602338
2216	94-95	-0.06000393203267862
2216	96-97	-0.11870845842157962
2216	98-99	-0.15425670527293533
2216	100	-0.11011030056458182
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28956.0
3	5060.0
4	3306.0
5	3029.0
6	2492.0
7	2831.0
8	3320.0
9	3823.0
10	4341.0
11	4593.0
12	5366.0
13	5895.0
14	6497.0
15	6817.0
16	6972.0
17	6902.0
18	7099.0
19	6735.0
20	6910.0
21	7264.0
22	8441.0
23	11155.0
24	17135.0
25	24157.0
26	25587.0
27	22287.0
28	23388.0
29	29694.0
30	37027.0
31	44668.0
32	59172.0
33	81350.0
34	114296.0
35	174928.0
36	333371.0
37	728734.0
38	1236831.0
39	565173.0
40	1286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19091784917568	19.537235182143466	13.104172703365968	24.167674265314883
2	15.430979474853535	19.738903907540323	40.91044245120046	23.919674166405688
3	18.316145669882246	24.93342793597948	29.732265415000885	27.01816097913739
4	11.972277104548512	16.377477275443074	39.271100867622735	32.37914475238568
5	12.737093655557802	38.079994630683515	36.164992984147695	13.017918729610988
6	31.411134939265477	38.25356992754186	17.58146869615994	12.753826437032718
7	27.62597395796827	32.27320814779643	22.46194101310447	17.638876881130834
8	24.933148370794974	37.14694920912695	20.19448341774113	17.725419002336942
9	24.85226677926168	16.84989268287893	21.047793691460754	37.25004684639864
10-11	23.788949338736316	26.850179256050904	29.2037136218369	20.15715778337588
12-13	24.420807783560036	24.15000280255948	29.097904517412214	22.33128489646827
14-15	21.63290951242434	26.386509994447945	25.895117042515697	26.085463450612018
16-17	21.41394637365563	29.70339983380451	26.582536053687715	22.300117738852144
18-19	21.304922823588647	28.781318203682666	28.4049726438579	21.508786328870784
20-21	22.51021992216861	28.111267821357956	27.88918662471906	21.489325631754376
22-23	22.115784828266396	27.883901505543164	27.959795085969084	22.040518580221356
24-25	22.04517699355234	27.993134716191925	27.878322337186145	22.083365953069585
26-27	21.76661778761178	27.984786394165933	28.007725727403255	22.24087009081903
28-29	21.723093255185397	27.98971635277749	28.107467413887203	22.17972297814991
30-31	21.962190278228785	28.481030698628967	27.6938339115702	21.862945111572053
32-33	21.63416247870444	28.18610987261603	28.200458078756103	21.97926956992343
34-35	21.82864513433173	27.88552976414605	28.2468325489173	22.038992552604924
36-37	22.16476231672666	28.296206373458414	27.695936580701364	21.843094729113563
38-39	21.89322604105994	28.413923684492964	27.889754216123492	21.803096058323604
40-41	21.639029674708162	28.097969111335182	28.183567919797586	22.07943329415907
42-43	21.702709163553067	27.910506114085504	28.185667576098616	22.201117146262813
44-45	21.75170951173639	28.149028076575654	28.15456462543474	21.94469778625321
46-47	21.863409244662705	28.372962219157603	27.928965421596498	21.83466311458319
48-49	21.76807827059394	28.16202947842558	27.95829128275701	22.11160096822347
50-51	21.724607590006865	28.11647294237199	27.996581063246783	22.162338404374363
52-53	21.806464281720718	28.01443295032741	28.116611802182394	22.06249096576947
54-55	21.954963436325425	28.420763978167063	27.816673821041714	21.80759876446579
56-57	21.77392346846385	28.15331506478716	27.889991098447247	22.182770368301746
58-59	21.634328504744772	27.76429786198869	28.314482056697283	22.286891576569253
60-61	21.967050957618362	28.21879466656194	27.82277671825727	21.991377657562428
62-63	21.74199398654131	28.244274600977697	27.902339249067644	22.111392163413353
64-65	21.722565247206386	27.897137684869755	28.106693443841905	22.27360362408195
66-67	21.616202688784995	28.02822388369661	27.8729805238785	22.482592903639894
68-69	22.089150813662044	28.166597646195108	27.820599863122652	21.923651677020192
70-71	21.974278715203894	27.9214684708701	27.908405336249587	22.195847477676427
72-73	21.759447193498772	28.08241154145565	28.03988146120756	22.11825980383802
74-75	21.878918601093158	28.09003257876201	27.905116878285895	22.125931941858937
76-77	21.852722943403	28.115777763899302	27.78166708438456	22.24983220831314
78-79	21.97223461206005	28.17707297137977	27.844858655169503	22.00583376139068
80-81	21.93256145308645	27.953216469960335	27.94150313335163	22.17271894360158
82-83	21.76890756818162	28.010853043609757	28.102376850912876	22.117862537295746
84-85	21.78513519575987	27.960983703529163	27.91860314327943	22.335277957431536
86-87	21.993577506272498	28.0196765572161	27.883576960837786	22.10316897567361
88-89	21.755424750382467	27.9614439954609	27.91270300287315	22.37042825128348
90-91	21.755399635521943	27.8558178262779	28.002769736879113	22.386012801321044
92-93	21.95786631347872	28.014811833168086	27.84535186198122	22.181969991371975
94-95	21.685147007564748	27.778426307054367	28.061732244407718	22.474694440973174
96-97	21.749707483618398	27.79502317497898	28.042588739113793	22.41268060228883
98-99	21.7729261846802	27.86922371142208	27.95935664481081	22.398493459086904
100	21.89423557810881	27.7054600133165	27.8639467054548	22.536357703119887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	248.5
2	254.0
3	222.0
4	184.5
5	160.0
6	133.5
7	137.5
8	172.5
9	182.5
10	172.0
11	174.0
12	201.5
13	230.0
14	274.0
15	337.5
16	429.0
17	586.0
18	876.0
19	1301.5
20	1964.0
21	3047.0
22	4748.0
23	7371.5
24	10825.5
25	15066.0
26	21067.0
27	29394.0
28	39331.5
29	50010.5
30	60153.5
31	70738.0
32	81588.0
33	91957.0
34	102554.0
35	111567.0
36	118578.0
37	124941.5
38	130445.5
39	132913.0
40	133289.5
41	134258.5
42	133362.5
43	131653.0
44	130192.0
45	127439.5
46	124975.5
47	121650.0
48	118291.5
49	116189.5
50	113682.0
51	109609.5
52	102558.0
53	96045.0
54	91569.5
55	86570.0
56	80019.5
57	73003.0
58	66590.0
59	60972.0
60	55446.0
61	47826.5
62	40632.0
63	34683.0
64	28304.0
65	22771.5
66	18580.5
67	14290.5
68	10488.0
69	7759.5
70	5449.0
71	3845.0
72	2869.0
73	2112.5
74	1546.5
75	1136.0
76	804.0
77	571.0
78	403.0
79	242.5
80	156.0
81	101.5
82	65.5
83	43.5
84	27.0
85	24.0
86	20.5
87	18.0
88	15.5
89	13.0
90	11.0
91	8.0
92	9.5
93	10.5
94	7.0
95	4.5
96	3.5
97	2.5
98	1.5
99	2.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3315890749867463
2	0.12012911220631772
3	0.0664868957001141
4	0.04008848920392442
5	0.04409733812431686
6	0.009735775949524501
7	0.007554089462236097
8	0.015271805411018826
9	0.017753473790309386
10-11	0.020821470413058703
12-13	0.01626719987084416
14-15	0.018476157439223668
16-17	0.0197306271694145
18-19	0.01934883203413903
20-21	0.024175813387264624
22-23	0.015544516221929877
24-25	0.024734870549632276
26-27	0.01898067243940911
28-29	0.018517064060860327
30-31	0.019485187439594556
32-33	0.025212114468726614
34-35	0.015612693924657637
36-37	0.010090300003708867
38-39	0.014085513383555756
40-41	0.009953944598253342
42-43	0.008549483922061432
44-45	0.009381251895340136
46-47	0.008385857435514801
48-49	0.00470426148821562
50-51	0.0068450413538673665
52-53	0.007581360543327202
54-55	0.0036543248662080758
56-57	0.0030543610822037653
58-59	0.0031634454065681853
60-61	0.003681595947299181
62-63	0.0030816321632948698
64-65	0.002277135271107271
66-67	0.0017589847303762754
68-69	0.0027680147307471625
70-71	0.0021953220278339565
72-73	0.0019498822980140106
74-75	0.002236228649470614
76-77	0.003790680271663601
78-79	0.003504333920206999
80-81	0.0034770628391158935
82-83	0.0022498641900161663
84-85	0.003067996622749318
86-87	0.002399855136017244
88-89	0.6030181450865149
90-91	0.613558417928227
92-93	0.6100404484674743
94-95	0.5969230584626528
96-97	0.5943186702184523
98-99	0.5922324325149828
100	0.5941550437319056
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3666888.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.61612958177057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.51834967480453	42.50744981594492
2	15.230873853865546	17.855497509235686
3	5.555529016012449	9.769308260936164
4	2.5251884952032233	5.920671042129136
5	1.3081207702082664	3.833848828756664
6	0.7628282461870706	2.6828423596281668
7	0.490468009663816	2.0124535487117137
8	0.3255134764704018	1.5264272093921358
9	0.2391657751438648	1.2617074860621647
>10	0.9892123655883966	9.795517008198882
>50	0.04170952936039289	1.6505631096032158
>100	0.01299423674452734	1.1678605122067132
>500	4.655074747620281E-5	0.015853309194383557
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.7271081091105046E-5	0.0
10-11	0.0	0.0	0.0	2.7271081091105046E-5	0.0
12-13	0.0	0.0	0.0	2.7271081091105046E-5	0.0
14-15	0.0	2.7271081091105046E-5	0.0	9.544878381886765E-5	0.0
16-17	0.0	2.7271081091105046E-5	0.0	1.3635540545552522E-4	0.0
18-19	0.0	2.7271081091105046E-5	0.0	1.636264865466303E-4	0.0
20-21	0.0	2.7271081091105046E-5	0.0	2.0453310818328786E-4	0.0
22-23	0.0	4.090662163665757E-5	0.0	2.5907527036549794E-4	0.0
24-25	0.0	5.454216218221009E-5	0.0	3.545240541843656E-4	0.0
26-27	0.0	5.454216218221009E-5	0.0	5.045150001854433E-4	0.0
28-29	0.0	5.454216218221009E-5	0.0	0.0011317498652808594	0.0
30-31	0.0	5.454216218221009E-5	0.0	0.00432246635294015	0.0
32-33	0.0	5.454216218221009E-5	0.0	0.011767471490811827	0.0
34-35	0.0	5.454216218221009E-5	0.0	0.0224031931163428	0.0
36-37	0.0	5.454216218221009E-5	0.0	0.04141113663684301	0.0
38-39	0.0	5.454216218221009E-5	0.0	0.07528181935199549	0.0
40-41	0.0	5.454216218221009E-5	0.0	0.11590209463719645	0.0
42-43	0.0	1.0908432436442019E-4	0.0	0.15645419221966966	0.0
44-45	0.0	1.0908432436442019E-4	0.0	0.20201053318236062	0.0
46-47	0.0	1.0908432436442019E-4	0.0	0.25944342996022784	0.0
48-49	0.0	1.0908432436442019E-4	0.0	0.3259712322819786	0.0
50-51	0.0	1.0908432436442019E-4	0.0	0.39739419365958273	0.0
52-53	0.0	1.0908432436442019E-4	0.0	0.4700852603079232	0.0
54-55	0.0	1.2271986490997272E-4	0.0	0.5474533173633882	0.0
56-57	0.0	1.3635540545552522E-4	0.0	0.6407204146949674	0.0
58-59	0.0	1.3635540545552522E-4	0.0	0.7473094351395515	0.0
60-61	0.0	1.3635540545552522E-4	0.0	0.85918904531581	0.0
62-63	0.0	1.3635540545552522E-4	0.0	0.9746411671150033	0.0
64-65	0.0	1.3635540545552522E-4	0.0	1.096174739997513	0.0
66-67	0.0	1.3635540545552522E-4	0.0	1.237288949103436	0.0
68-69	0.0	1.3635540545552522E-4	0.0	1.396565698216035	0.0
70-71	0.0	1.4999094600107773E-4	0.0	1.57326866814585	0.0
72-73	0.0	1.6362648654663026E-4	0.0	1.7570893902404436	0.0
74-75	0.0	1.6362648654663026E-4	0.0	1.9394647450372087	0.0
76-77	0.0	1.6362648654663026E-4	0.0	2.137698233488451	0.0
78-79	0.0	1.6362648654663026E-4	0.0	2.369843311276483	0.0
80-81	0.0	1.6362648654663026E-4	0.0	2.624255226775402	0.0
82-83	0.0	1.6362648654663026E-4	0.0	2.886534849169105	0.0
84-85	0.0	1.6362648654663026E-4	0.0	3.145296502102055	0.0
86-87	0.0	1.6362648654663026E-4	0.0	3.424211483961332	0.0
88	0.0	1.6362648654663026E-4	0.0	3.6505614570175036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCAACG	3830	0.0	32.752483	3
TCAACGC	3900	0.0	32.158913	4
GGTATCA	2640	0.0	31.955978	1
GTATCAA	4290	0.0	31.640123	1
CAACGCA	3965	0.0	31.512388	5
AACGCAG	4355	0.0	28.788422	6
GTACATG	8880	0.0	28.395124	1
TACATGG	8955	0.0	26.246614	2
TATCAAC	5260	0.0	24.844368	2
ACATGGG	9655	0.0	23.843805	3
ACGCAGA	5205	0.0	23.545858	7
CGCAGAG	5265	0.0	23.009972	8
GTATAGG	1340	0.0	21.806696	1
CATGGGG	6685	0.0	20.377518	4
GAGTACA	6840	0.0	19.775558	1
GTCGTAC	300	4.101821E-9	18.852242	1
GTACCGT	155	0.0010403743	18.17667	6
TACCGTC	315	7.863491E-9	17.888151	7
TATACTG	2350	0.0	17.58967	5
AGTACAT	6960	0.0	17.290184	2
>>END_MODULE