FastQCFastQC Report
Wed 25 May 2016
SRR1033190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666888
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51600.14071877843010205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43310.11811105220557595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45700.052.1108671
GTATCAA85700.041.9572031
CCGCGTA704.6421246E-640.280549
ATCAACG97150.036.685433
TCAACGC97300.036.6288724
CAACGCA99300.035.8906445
AACGCAG104050.034.2386476
TATCAAC106100.033.6351662
ACGCAGA120450.029.3427317
CGCAGAG122500.028.8872998
GTACATG92650.027.0754551
GCAGAGT138550.025.4048949
TACATGG92150.025.3587742
ACATGGG95600.023.9518073
GAGTACT93550.021.7513512-13
CAGAGTA134650.021.0626510-11
AGAGTAC126400.020.29960810-11
GAGTACA68550.019.9792651
GTGGTAT25600.019.487671
GTACTTT105900.019.10377914-15
Produced by FastQC (version 0.11.5)