FastQCFastQC Report
Wed 25 May 2016
SRR1033189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086082
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63550.034.9871061
TCAACGC66150.032.1710324
CAACGCA66750.031.67045
ATCAACG68500.031.3463023
AACGCAG69900.030.2324896
GGTATCA47850.029.1401231
TATCAAC75350.028.698672
GTACATG171400.027.4284531
TACATGG182300.025.142312
ACATGGG176600.024.4503773
ACGCAGA88250.023.839767
CATGGGG148650.021.4586054
CGCAGAG97500.021.4335448
GAGTACA112750.020.4302621
GTATAGA16150.020.4177151
GGACCGT5800.019.4321926
GCAGAGT112000.018.3232279
AGTACAT122000.018.2258822
ATGGGGG64000.018.0570775
GTCTAGG9350.017.1296651
Produced by FastQC (version 0.11.5)