##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033189_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5086082
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865037960457578	33.0	31.0	34.0	30.0	34.0
2	31.91465080586589	34.0	31.0	34.0	30.0	34.0
3	32.08841383210102	34.0	31.0	34.0	30.0	34.0
4	35.484568278686815	37.0	35.0	37.0	35.0	37.0
5	35.53577586834031	37.0	35.0	37.0	35.0	37.0
6	35.61517863848833	37.0	37.0	37.0	35.0	37.0
7	35.541749228581054	37.0	36.0	37.0	35.0	37.0
8	35.54813233447671	37.0	37.0	37.0	35.0	37.0
9	37.26668425715511	39.0	38.0	39.0	35.0	39.0
10-11	37.261640197700316	39.0	38.0	39.0	35.0	39.0
12-13	37.22965791743035	39.0	38.0	39.0	35.0	39.0
14-15	38.714902453401265	41.0	39.0	41.0	36.0	41.0
16-17	38.70966394171388	41.0	39.0	41.0	35.5	41.0
18-19	38.67664314102683	41.0	39.0	41.0	35.0	41.0
20-21	38.688931381759076	41.0	39.0	41.0	35.0	41.0
22-23	38.64111874326839	41.0	39.0	41.0	35.0	41.0
24-25	38.61732842687161	41.0	39.0	41.0	35.0	41.0
26-27	38.56363247780905	41.0	39.0	41.0	34.0	41.0
28-29	38.52534544271995	40.5	39.0	41.0	34.5	41.0
30-31	38.52008471353785	41.0	39.0	41.0	34.0	41.0
32-33	38.58843831066821	41.0	39.0	41.0	35.0	41.0
34-35	38.55210680834482	41.0	39.0	41.0	34.5	41.0
36-37	38.474309006421834	41.0	39.0	41.0	34.0	41.0
38-39	38.3909731498627	41.0	39.0	41.0	34.0	41.0
40-41	38.315155850810115	41.0	38.5	41.0	34.0	41.0
42-43	38.19133234580174	40.5	38.0	41.0	33.5	41.0
44-45	38.04677107054114	40.0	38.0	41.0	33.0	41.0
46-47	37.90311039027684	40.0	38.0	41.0	33.0	41.0
48-49	37.7639095280021	40.0	38.0	41.0	33.0	41.0
50-51	37.61126600003696	40.0	37.0	41.0	33.0	41.0
52-53	37.44128997526977	40.0	37.0	41.0	32.5	41.0
54-55	37.26197503304115	40.0	36.0	41.0	32.0	41.0
56-57	37.07379000181279	40.0	36.0	41.0	32.0	41.0
58-59	36.850034958146566	39.0	35.0	41.0	31.5	41.0
60-61	36.601822286781854	39.0	35.0	41.0	31.0	41.0
62-63	36.31731379871579	39.0	35.0	41.0	31.0	41.0
64-65	36.018043063403226	38.0	35.0	40.5	31.0	41.0
66-67	35.70400133147676	37.0	35.0	40.0	30.5	41.0
68-69	35.37679357116146	37.0	35.0	40.0	30.0	41.0
70-71	35.01737653856151	36.0	35.0	39.0	30.0	41.0
72-73	34.64972821908888	36.0	34.0	39.0	29.5	41.0
74-75	34.27724199098638	35.0	34.0	38.5	29.0	40.0
76-77	33.95993664671549	35.0	34.0	37.0	29.0	39.5
78-79	33.61705218280004	35.0	34.0	37.0	29.0	39.0
80-81	33.29027314148691	35.0	34.0	36.5	29.0	39.0
82-83	33.11536080228356	35.0	34.0	36.0	29.0	37.0
84-85	32.88867462223378	35.0	34.0	36.0	29.0	37.0
86-87	32.683159846813325	35.0	34.0	35.0	29.0	37.0
88-89	32.37380227058864	35.0	34.0	35.0	27.5	36.0
90-91	32.220845731547385	35.0	34.0	35.0	27.0	36.0
92-93	32.077328383616305	35.0	34.0	35.0	27.0	36.0
94-95	31.934579013865683	35.0	33.0	35.0	27.0	35.5
96-97	31.783174357000142	35.0	33.0	35.0	27.0	35.0
98-99	31.61220119927284	35.0	33.0	35.0	25.5	35.0
100	31.46439341717259	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22943720209621077
1101	2	0.20198498628293038
1101	3	0.23263827275630433
1101	4	0.19775532828192155
1101	5	0.1924955569539364
1101	6	0.19819140240926458
1101	7	0.25226917786638126
1101	8	0.16872829042289084
1101	9	0.25984870335398114
1101	10-11	0.26098211732222865
1101	12-13	0.28628932743202995
1101	14-15	0.35006530517145507
1101	16-17	0.2513413165512688
1101	18-19	0.3065204646670665
1101	20-21	0.3117215434163185
1101	22-23	0.329142918805438
1101	24-25	0.29984712545062564
1101	26-27	0.2621727114140313
1101	28-29	0.3195171155158505
1101	30-31	0.3100314081246154
1101	32-33	0.3322573402295177
1101	34-35	0.3628050126424611
1101	36-37	0.3429971593261314
1101	38-39	0.32817826895402646
1101	40-41	0.34446578266550887
1101	42-43	0.36542447398408484
1101	44-45	0.3234255473315173
1101	46-47	0.347427197529683
1101	48-49	0.322610010656625
1101	50-51	0.3092332364112096
1101	52-53	0.3113378229794108
1101	54-55	0.24010671394341188
1101	56-57	0.2928654961287762
1101	58-59	0.29131072434714156
1101	60-61	0.29124219984723254
1101	62-63	0.34087591313915766
1101	64-65	0.31476385334054413
1101	66-67	0.2880050797195324
1101	68-69	0.30084321112679646
1101	70-71	0.30945724934124286
1101	72-73	0.3332483061718392
1101	74-75	0.2601195540569208
1101	76-77	0.2609517621517483
1101	78-79	0.2973863207061811
1101	80-81	0.32434101507497815
1101	82-83	0.3097226649141973
1101	84-85	0.2296295328839335
1101	86-87	0.33363964236557564
1101	88-89	0.4539205598035352
1101	90-91	0.5194599103764119
1101	92-93	0.5129681886211017
1101	94-95	0.4593011860302809
1101	96-97	0.4534073892769612
1101	98-99	0.48978932506430084
1101	100	0.5029600359902489
1102	1	0.18922290933113217
1102	2	0.1730633897958498
1102	3	0.18171667028673255
1102	4	0.1799151821040681
1102	5	0.08841402502645934
1102	6	0.10889851981946208
1102	7	0.11033201366029743
1102	8	0.14595647922854482
1102	9	0.17983293094837904
1102	10-11	0.16466453175233653
1102	12-13	0.20734200624115573
1102	14-15	0.21819002554349254
1102	16-17	0.265679890402744
1102	18-19	0.2246051023611031
1102	20-21	0.2251346368632241
1102	22-23	0.20443278109280527
1102	24-25	0.26581572464648673
1102	26-27	0.2846922111241028
1102	28-29	0.2661117544363023
1102	30-31	0.2701542286289964
1102	32-33	0.3180844813303736
1102	34-35	0.2901212722363411
1102	36-37	0.27935255422811167
1102	38-39	0.16786915800747693
1102	40-41	0.21005561551048402
1102	42-43	0.20783347498102955
1102	44-45	0.21911624280871678
1102	46-47	0.2635059279429086
1102	48-49	0.23692913073501387
1102	50-51	0.2038617836534371
1102	52-53	0.2093011913430729
1102	54-55	0.2707540449199257
1102	56-57	0.19151463937220825
1102	58-59	0.22529186155852443
1102	60-61	0.17560471006554224
1102	62-63	0.23504595599514744
1102	64-65	0.23866701735092022
1102	66-67	0.1552169149634821
1102	68-69	0.17586574160864643
1102	70-71	0.21981979673643792
1102	72-73	0.22280902266894032
1102	74-75	0.14987654961663566
1102	76-77	0.14255722083133548
1102	78-79	0.12959967507508452
1102	80-81	0.1448301006643149
1102	82-83	0.17478055831734451
1102	84-85	0.11785672047727758
1102	86-87	0.19341910003583251
1102	88-89	0.3190090600490336
1102	90-91	0.32332988652764527
1102	92-93	0.3395539969600918
1102	94-95	0.33478191109089295
1102	96-97	0.32310796141658926
1102	98-99	0.3916280004044381
1102	100	0.3918894609918411
1103	1	0.15502671743641017
1103	2	0.11891573369520358
1103	3	0.1561051221347114
1103	4	0.12652639967298995
1103	5	0.0898998146410932
1103	6	0.14131127502777474
1103	7	0.11418964525866215
1103	8	0.09071557546083397
1103	9	0.04991312282516702
1103	10-11	0.12550356061252899
1103	12-13	0.16940569056731647
1103	14-15	0.1770868685360938
1103	16-17	0.12173874275357122
1103	18-19	0.15715651088557792
1103	20-21	0.12023522013246435
1103	22-23	0.1394966313219541
1103	24-25	0.14583385168741358
1103	26-27	0.1889452074664888
1103	28-29	0.2179539297464359
1103	30-31	0.2075426975971908
1103	32-33	0.1727966661503757
1103	34-35	0.21258261011549706
1103	36-37	0.22638211634614436
1103	38-39	0.24243471230890634
1103	40-41	0.2600782794069403
1103	42-43	0.17342526763408728
1103	44-45	0.13521108428576412
1103	46-47	0.194005020775613
1103	48-49	0.20042326971280033
1103	50-51	0.18049234094478805
1103	52-53	0.07441226277877888
1103	54-55	0.13559992634780826
1103	56-57	0.08838547269025554
1103	58-59	0.09174252824801954
1103	60-61	0.04570217501089502
1103	62-63	0.09857437615443843
1103	64-65	0.1499384232176908
1103	66-67	0.15819966309137357
1103	68-69	0.1346052926759853
1103	70-71	0.14447563976205657
1103	72-73	0.14468210810978377
1103	74-75	0.10959766955551942
1103	76-77	0.09385187553505148
1103	78-79	0.1028850962791239
1103	80-81	0.11081508746340774
1103	82-83	0.15987978012230286
1103	84-85	0.1351453093277044
1103	86-87	0.11616057753437303
1103	88-89	0.22978507023310613
1103	90-91	0.2089383836137415
1103	92-93	0.22625665886734225
1103	94-95	0.2152784868451718
1103	96-97	0.22913265487312628
1103	98-99	0.3076307494209445
1103	100	0.35157055225030476
1104	1	0.1324219547425578
1104	2	0.03711765267937395
1104	3	0.04824221656932792
1104	4	0.08550452737534897
1104	5	0.04092422132351459
1104	6	-0.004763051143832797
1104	7	-0.024864527912228596
1104	8	0.002130356308498449
1104	9	-0.006432842049001408
1104	10-11	0.03704517674235319
1104	12-13	0.0036814692291926576
1104	14-15	0.009957537395514748
1104	16-17	0.056538503147457675
1104	18-19	0.03650923551168006
1104	20-21	0.03205708073294744
1104	22-23	0.006923416148509887
1104	24-25	0.005235258781553398
1104	26-27	0.08820149782342668
1104	28-29	0.11403707520572937
1104	30-31	0.11448859451643045
1104	32-33	0.13493830715987798
1104	34-35	0.11030225563583684
1104	36-37	0.12078948110120535
1104	38-39	0.08854678735269061
1104	40-41	0.09236037685636234
1104	42-43	0.1186715903392539
1104	44-45	0.0703139496723324
1104	46-47	-0.047659069730705994
1104	48-49	-0.061766204328087326
1104	50-51	-0.004716239850793613
1104	52-53	-0.0752950297045416
1104	54-55	-0.1140131274048315
1104	56-57	-0.11397389564449867
1104	58-59	-0.191917559694204
1104	60-61	-0.10839880536897795
1104	62-63	-0.09544757412427174
1104	64-65	-0.11916502424187314
1104	66-67	-0.13342331328825452
1104	68-69	-0.1278098855037939
1104	70-71	-0.12648368753981032
1104	72-73	-0.008233200270531427
1104	74-75	-0.054867540443325424
1104	76-77	-0.13771198376667115
1104	78-79	-0.12152341962507762
1104	80-81	-0.07084082895573829
1104	82-83	-0.050332931813308335
1104	84-85	-0.05561907458238835
1104	86-87	-0.0203561357120563
1104	88-89	0.007551901263454397
1104	90-91	-0.03284720418481868
1104	92-93	-0.10628937897483226
1104	94-95	-0.10189199323507836
1104	96-97	-0.0793159558337635
1104	98-99	-0.0686766519573716
1104	100	-0.016561833613028654
1105	1	0.1687096009690201
1105	2	0.12609761149398224
1105	3	0.1538514765248351
1105	4	0.1510982911308929
1105	5	0.10724568226528675
1105	6	0.09809776350664379
1105	7	0.060879992388976234
1105	8	0.05836132539511141
1105	9	0.06620691275507085
1105	10-11	0.09606099733231588
1105	12-13	0.12940046845137942
1105	14-15	0.14612657215363356
1105	16-17	0.1367935922125625
1105	18-19	0.13981926405362088
1105	20-21	0.14305809494802446
1105	22-23	0.24272581525389114
1105	24-25	0.24075686441291566
1105	26-27	0.2882740986855836
1105	28-29	0.2952924588975563
1105	30-31	0.29949230434885976
1105	32-33	0.3031141981589087
1105	34-35	0.2661596306984677
1105	36-37	0.26390531764531033
1105	38-39	0.20556666279896518
1105	40-41	0.21944628780234154
1105	42-43	0.22242629591460883
1105	44-45	0.2671671162749263
1105	46-47	0.18982919428036382
1105	48-49	0.18834139992473098
1105	50-51	0.29600365282625773
1105	52-53	0.24920375139292616
1105	54-55	0.20728818933054072
1105	56-57	0.20846655285165383
1105	58-59	0.20525263215755274
1105	60-61	0.14863461422218194
1105	62-63	0.2442605828792992
1105	64-65	0.25405329408049226
1105	66-67	0.25578206706541096
1105	68-69	0.22649750958224502
1105	70-71	0.20902291213202773
1105	72-73	0.20286432748140726
1105	74-75	0.2674928032831474
1105	76-77	0.2612178873928741
1105	78-79	0.22613912059755847
1105	80-81	0.20678113078805893
1105	82-83	0.1987647066045639
1105	84-85	0.17104769748040383
1105	86-87	0.23444823923675528
1105	88-89	0.2615907347643258
1105	90-91	0.28200517758772037
1105	92-93	0.2649694063399721
1105	94-95	0.2721377082849372
1105	96-97	0.3261539400070248
1105	98-99	0.3050788433467311
1105	100	0.3202211076225794
1106	1	0.21413957214723567
1106	2	0.1545100572986371
1106	3	0.13810937490014652
1106	4	0.14861632906890065
1106	5	0.0555714891619985
1106	6	0.09739982728203955
1106	7	0.09439162603276685
1106	8	0.055512319149485734
1106	9	0.13039754920986013
1106	10-11	0.11216158725042646
1106	12-13	0.07625410118943421
1106	14-15	0.12873695321860623
1106	16-17	0.11719726367129368
1106	18-19	0.1236006712275568
1106	20-21	0.08472923387902398
1106	22-23	0.09646239868004614
1106	24-25	0.14913051241131825
1106	26-27	0.19395242210980257
1106	28-29	0.20814000468077154
1106	30-31	0.23093822524930374
1106	32-33	0.19397893567102642
1106	34-35	0.1806103188778181
1106	36-37	0.1782325590562408
1106	38-39	0.19150198026894572
1106	40-41	0.20420192684406402
1106	42-43	0.21334612950253984
1106	44-45	0.238130511266192
1106	46-47	0.22002881301727228
1106	48-49	0.20172934309749024
1106	50-51	0.2120185961794263
1106	52-53	0.11739812368601577
1106	54-55	0.1467103125834086
1106	56-57	0.13423811268686592
1106	58-59	0.09692190387070809
1106	60-61	0.08253313014341046
1106	62-63	0.13359713846373467
1106	64-65	0.09872434201749769
1106	66-67	0.16213873407630075
1106	68-69	0.1316969398441188
1106	70-71	0.11499689591405371
1106	72-73	0.12275715794346098
1106	74-75	0.1251959184512117
1106	76-77	0.12563281791220504
1106	78-79	0.15639874452761404
1106	80-81	0.11291528328943912
1106	82-83	0.05326617434298697
1106	84-85	0.04206948466912053
1106	86-87	0.08918608974115472
1106	88-89	0.20257369434142447
1106	90-91	0.15511065716202665
1106	92-93	0.18443659879648067
1106	94-95	0.1687575969075823
1106	96-97	0.18221658595464163
1106	98-99	0.1701324763302452
1106	100	0.24804575675656793
1107	1	0.14044839968698497
1107	2	0.11552769481026104
1107	3	0.1268528760807186
1107	4	0.07832934981986739
1107	5	0.056854294704834274
1107	6	0.08189915036778217
1107	7	0.0393849069886727
1107	8	0.0352556244449147
1107	9	0.04515822115100576
1107	10-11	0.06498850377279553
1107	12-13	0.02672847555516711
1107	14-15	-0.008798764925884939
1107	16-17	0.02976377986988865
1107	18-19	0.09218574591523776
1107	20-21	0.07600913036883838
1107	22-23	0.08645108142843583
1107	24-25	0.05002761793696209
1107	26-27	0.09010265282175567
1107	28-29	0.09200968979332202
1107	30-31	0.11571017603320399
1107	32-33	0.1245757858446126
1107	34-35	0.0685430259019455
1107	36-37	0.10419906353179442
1107	38-39	0.12392700827818715
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2216	16-17	-0.21024372274404612
2216	18-19	-0.2518380817336592
2216	20-21	-0.22538591172821754
2216	22-23	-0.17708277422342178
2216	24-25	-0.1877094171622531
2216	26-27	-0.3097340572557883
2216	28-29	-0.22525064915136994
2216	30-31	-0.26731455467198373
2216	32-33	-0.17731447246696774
2216	34-35	-0.20785536270553706
2216	36-37	-0.22773477897311523
2216	38-39	-0.21339933283844204
2216	40-41	-0.19244213164204638
2216	42-43	-0.20984293185956204
2216	44-45	-0.1629592228870962
2216	46-47	-0.13386555345230988
2216	48-49	-0.13186379925779335
2216	50-51	-0.17295866995140585
2216	52-53	-0.19737189259599575
2216	54-55	-0.1523951091779452
2216	56-57	-0.22508572341110522
2216	58-59	-0.26538027918737583
2216	60-61	-0.19545691142288035
2216	62-63	-0.1641138215180291
2216	64-65	-0.13448385926163553
2216	66-67	-0.14151827571655673
2216	68-69	-0.13832099791470398
2216	70-71	-0.2057724185524492
2216	72-73	-0.1327943641363234
2216	74-75	-0.1016045569578381
2216	76-77	-0.2322704539895426
2216	78-79	-0.20750193327256028
2216	80-81	-0.16248437170228414
2216	82-83	-0.18770165206314715
2216	84-85	-0.228906798586074
2216	86-87	-0.2187918996325493
2216	88-89	-0.1761205141779243
2216	90-91	-0.15030649869305535
2216	92-93	-0.150751907680565
2216	94-95	-0.13041334525886583
2216	96-97	-0.1384530217327118
2216	98-99	-0.20929382427553378
2216	100	-0.20239571636587428
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41766.0
3	7345.0
4	4951.0
5	4121.0
6	3890.0
7	4515.0
8	5352.0
9	5746.0
10	6221.0
11	6771.0
12	7420.0
13	8115.0
14	8640.0
15	9129.0
16	9232.0
17	9433.0
18	9599.0
19	9300.0
20	9589.0
21	10124.0
22	11272.0
23	15034.0
24	21657.0
25	29827.0
26	31688.0
27	30009.0
28	33383.0
29	42437.0
30	52498.0
31	66031.0
32	88627.0
33	122026.0
34	174438.0
35	280934.0
36	556337.0
37	1153860.0
38	1632748.0
39	561332.0
40	685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97670112207766	18.42252227496061	12.94181770622401	24.658958896737726
2	15.739208968727091	19.43478137625363	39.50585545187333	25.32015420314595
3	18.842535737431156	24.563928380728132	29.203941318565523	27.389594563275192
4	12.921466108639132	16.168846469914623	36.71142845790934	34.198258963536894
5	14.207953490878863	37.38877154691688	34.37089513456779	14.032379827636468
6	32.84496700975992	36.14652759411632	17.453985226900688	13.55452016922307
7	28.995859593887452	31.510167757528755	21.595785700900933	17.898186947682863
8	26.41517522406298	35.83772721668326	19.51904798331029	18.228049575943473
9	26.138234732503555	16.433945778802638	19.587216302897094	37.84060318579671
10-11	25.365526775863867	26.000896756925773	27.674125219518626	20.959451247691735
12-13	25.70217230246757	23.01382079328099	27.937155776804488	23.346851127446953
14-15	22.893745539091153	24.72946452968464	24.770190986631356	27.606598944592847
16-17	23.358909543714688	28.044406127435174	25.32865859702061	23.268025731829532
18-19	23.46915357657177	27.321158629000653	26.627885230494947	22.581802563932634
20-21	24.273611765972625	26.545397513094088	26.285093953438977	22.89589676749431
22-23	23.91011045053127	26.337919985652658	26.157870300906755	23.594099262909314
24-25	23.811118668507582	26.377566588043305	26.248692055257735	23.562622688191382
26-27	23.592028499787762	26.625779816504743	26.146869952462005	23.63532173124549
28-29	23.59621423188051	26.71851424677698	26.143028722654776	23.542242798687735
30-31	23.69684407595614	26.864174126457215	25.88218578418262	23.556796013404025
32-33	23.60801431331066	26.624517985300244	26.26157191735528	23.505895784033815
34-35	23.585079858816226	26.59204878702028	26.299331778127765	23.52353957603573
36-37	23.802488536993238	26.495613053212914	26.00488418423122	23.697014225562636
38-39	23.7944053510101	26.73112904700618	26.01536360499037	23.45910199699335
40-41	23.686957961438015	26.631809453527104	26.235534045166116	23.44569853986876
42-43	23.612013019731638	26.533862677362478	26.185923946433366	23.66820035647252
44-45	23.66317319893909	26.476519301573877	26.24877977369392	23.611527725793113
46-47	23.622939980779297	26.56605702796833	26.180050288433414	23.630952702818956
48-49	23.731946874248834	26.51118321738271	26.03520645240257	23.72166345596588
50-51	23.63408436048517	26.69746975173357	26.162602300626105	23.50584358715516
52-53	23.68083367677918	26.534455745822804	26.162934511431242	23.62177606596677
54-55	23.652303957095516	26.63591433731912	26.112467365670827	23.59931433991453
56-57	23.427068965686743	26.553917814521817	26.30237114709488	23.716642072696565
58-59	23.43152119051556	26.340900024007404	26.414023312545925	23.81355547293111
60-61	23.56851933577492	26.638813825645098	26.224530916034173	23.568135922545807
62-63	23.563788591001884	26.643823267038712	26.122306369352486	23.670081772606913
64-65	23.538292051968256	26.58901943805593	26.128122351159107	23.74456615881671
66-67	23.499731566070384	26.48412110818728	26.146742767349824	23.869404558392514
68-69	23.462823498067944	26.470143234024608	26.28518115990116	23.78185210800629
70-71	23.41209606858109	26.43492366224599	26.285344920811255	23.867635348361663
72-73	23.51016333734895	26.501358539372085	26.21969190973534	23.76878621354363
74-75	23.514649073634775	26.581635732772206	26.17469135086384	23.72902384272918
76-77	23.568107250989485	26.426234910505382	26.18676726444442	23.818890574060713
78-79	23.591045935604406	26.403443722698373	26.280653057580956	23.724857284116265
80-81	23.584744837919065	26.310091557319915	26.412689236976455	23.692474367784563
82-83	23.49622336024054	26.31860425312034	26.49163040081212	23.693541985826997
84-85	23.584619167319698	26.35536689021602	26.203466583063	23.85654735940128
86-87	23.541814459616543	26.48737382873268	26.30171507983572	23.669096631815055
88-89	23.438521133558137	26.24321156344498	26.30971134111973	24.008555961877153
90-91	23.47552176277412	26.313879998567792	26.296175187033032	23.914423051625057
92-93	23.597614197709444	26.30236745529093	26.38405363851663	23.71596470848299
94-95	23.491684468873846	26.163518703739562	26.35952263647639	23.985274190910204
96-97	23.53018369848283	26.071976641086124	26.459739279046897	23.93810038138415
98-99	23.324732366476937	26.33917133064667	26.449708571501468	23.886387731374924
100	23.63252096903037	25.997602522236797	26.286221504240288	24.083655004492552
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	444.0
1	432.0
2	393.0
3	360.0
4	303.5
5	242.5
6	226.5
7	221.5
8	219.5
9	218.0
10	228.0
11	237.0
12	258.0
13	276.5
14	300.5
15	373.0
16	461.5
17	567.5
18	727.5
19	990.0
20	1409.0
21	2080.0
22	3235.5
23	5186.0
24	7756.5
25	11059.0
26	15572.0
27	21589.0
28	29459.0
29	39007.5
30	48784.5
31	58466.5
32	68935.5
33	81838.5
34	94441.5
35	103388.0
36	110525.0
37	121501.5
38	134071.0
39	142999.5
40	151890.5
41	160593.5
42	167319.5
43	171729.5
44	174153.5
45	179450.5
46	179549.0
47	171527.0
48	170894.0
49	175237.5
50	176153.0
51	176706.0
52	175853.0
53	176641.0
54	179733.5
55	176034.5
56	163490.5
57	152014.0
58	138084.5
59	120318.0
60	106805.0
61	95612.5
62	83817.0
63	69234.5
64	56366.5
65	47255.0
66	39296.5
67	32143.0
68	26784.5
69	22261.5
70	17518.5
71	12524.5
72	9022.5
73	6949.0
74	5094.0
75	3285.0
76	1993.5
77	1351.5
78	935.0
79	627.0
80	393.0
81	232.5
82	140.0
83	97.0
84	63.5
85	55.0
86	48.5
87	37.0
88	40.5
89	32.0
90	24.5
91	22.0
92	19.0
93	15.0
94	11.5
95	8.5
96	7.5
97	5.5
98	4.0
99	3.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3308047333880972
2	0.11855884352631356
3	0.06519753318959466
4	0.03900841551512539
5	0.04278342346820204
6	0.009575150380980882
7	0.006861863414707037
8	0.015591569306196793
9	0.018658763268071574
10-11	0.021470357733123453
12-13	0.015915984052164316
14-15	0.01839333302137087
16-17	0.020015406751208494
18-19	0.019720484254874382
20-21	0.024567043944631644
22-23	0.01610276829984259
24-25	0.02539282693436716
26-27	0.019494377007684895
28-29	0.01935674650939564
30-31	0.020487282745343078
32-33	0.02564842643119006
34-35	0.015630892305708008
36-37	0.009506335131836253
38-39	0.013507450332102391
40-41	0.009388366133302609
42-43	0.008189014648210547
44-45	0.008798521140634383
46-47	0.008247999147477371
48-49	0.004414006695133897
50-51	0.00660626391788414
52-53	0.007274754909574797
54-55	0.00353906995600936
56-57	0.002890240464074311
58-59	0.0029688864630967415
60-61	0.0036472082046652018
62-63	0.002929563463585526
64-65	0.0025756564679845903
66-67	0.0019759807254385595
68-69	0.0034309317073535186
70-71	0.0025953179677401976
72-73	0.0020644574743387935
74-75	0.002536333468473375
76-77	0.004276376196844644
78-79	0.00374551570344324
80-81	0.0038733154518546887
82-83	0.0028607482144408997
84-85	0.003548900705887164
86-87	0.002713286966273843
88-89	0.5982207915641156
90-91	0.6087003709338544
92-93	0.6047779017326107
94-95	0.5910443441533189
96-97	0.590336530162117
98-99	0.5872398439506088
100	0.5885669951841123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5086082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.05878088558012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.603326987590656	20.30016653921934
2	15.500316534634774	10.558437690205205
3	7.480449897001487	7.643250119026021
4	4.511403353609183	6.14611593228186
5	2.9053546269312505	4.947641831677892
6	1.9906710680044657	4.067989783225672
7	1.436380378263185	3.4244955200128753
8	1.090431409967345	2.971101157026559
9	0.8611718393385059	2.6397416682775963
>10	4.318355743936935	25.749194283559778
>50	0.21001991605524595	4.838517715807153
>100	0.08668034285753488	5.218855405762613
>500	0.0043966029904047455	0.9924997683962994
>1k	0.0010412988190784554	0.5019925855213224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	5.898449926682267E-5	0.0
9	0.0	0.0	0.0	5.898449926682267E-5	0.0
10-11	9.830749877803779E-6	0.0	0.0	5.898449926682267E-5	0.0
12-13	1.9661499755607557E-5	0.0	0.0	7.864599902243023E-5	0.0
14-15	1.9661499755607557E-5	0.0	0.0	1.1796899853364534E-4	0.0
16-17	1.9661499755607557E-5	0.0	0.0	1.376304982892529E-4	0.0
18-19	1.9661499755607557E-5	0.0	0.0	1.76953497800468E-4	0.0
20-21	1.9661499755607557E-5	0.0	0.0	1.9661499755607557E-4	0.0
22-23	1.9661499755607557E-5	0.0	0.0	2.0644574743387936E-4	0.0
24-25	1.9661499755607557E-5	0.0	0.0	3.047532462119171E-4	0.0
26-27	1.9661499755607557E-5	0.0	0.0	5.013682437679927E-4	0.0
28-29	1.9661499755607557E-5	0.0	0.0	0.0013861357327703328	0.0
30-31	1.9661499755607557E-5	0.0	0.0	0.005259451184625022	0.0
32-33	1.9661499755607557E-5	0.0	0.0	0.015041047313039782	0.0
34-35	1.9661499755607557E-5	0.0	0.0	0.029246480886466242	0.0
36-37	1.9661499755607557E-5	0.0	0.0	0.052791126843806296	0.0
38-39	1.9661499755607557E-5	9.830749877803779E-6	0.0	0.09423756832862704	0.0
40-41	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.14156279824037443	0.0
42-43	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.19110977762450548	0.0
44-45	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.2494651088991487	0.0
46-47	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.3192142792821665	0.0
48-49	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.395894128329036	0.0
50-51	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.4766734000749496	0.0
52-53	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.5593500065472794	0.0
54-55	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.6513363331538894	0.0
56-57	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.7574297858351478	0.0
58-59	1.9661499755607557E-5	1.9661499755607557E-5	0.0	0.8758509988631721	0.0
60-61	1.9661499755607557E-5	1.9661499755607557E-5	0.0	1.0034934552765764	0.0
62-63	1.9661499755607557E-5	1.9661499755607557E-5	0.0	1.1353631341374362	0.0
64-65	1.9661499755607557E-5	1.9661499755607557E-5	0.0	1.2782727451110698	0.0
66-67	1.9661499755607557E-5	1.9661499755607557E-5	0.0	1.4409913170884778	0.0
68-69	1.9661499755607557E-5	1.9661499755607557E-5	0.0	1.6229879895762593	0.0
70-71	1.9661499755607557E-5	1.9661499755607557E-5	0.0	1.8168503771665498	0.0
72-73	1.9661499755607557E-5	1.9661499755607557E-5	0.0	2.016188492438777	0.0
74-75	1.9661499755607557E-5	1.9661499755607557E-5	0.0	2.222191856128155	0.0
76-77	1.9661499755607557E-5	1.9661499755607557E-5	0.0	2.452663956263387	0.0
78-79	1.9661499755607557E-5	1.9661499755607557E-5	0.0	2.7082241300867738	0.0
80-81	1.9661499755607557E-5	1.9661499755607557E-5	0.0	2.9835539419144244	0.0
82-83	1.9661499755607557E-5	1.9661499755607557E-5	0.0	3.271299990837741	0.0
84-85	1.9661499755607557E-5	1.9661499755607557E-5	0.0	3.561356265982342	0.0
86-87	2.9492249633411336E-5	1.9661499755607557E-5	0.0	3.872902560359821	0.0
88	3.9322999511215114E-5	1.9661499755607557E-5	0.0	4.126830829703493	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6355	0.0	34.987106	1
TCAACGC	6615	0.0	32.171032	4
CAACGCA	6675	0.0	31.6704	5
ATCAACG	6850	0.0	31.346302	3
AACGCAG	6990	0.0	30.232489	6
GGTATCA	4785	0.0	29.140123	1
TATCAAC	7535	0.0	28.69867	2
GTACATG	17140	0.0	27.428453	1
TACATGG	18230	0.0	25.14231	2
ACATGGG	17660	0.0	24.450377	3
ACGCAGA	8825	0.0	23.83976	7
CATGGGG	14865	0.0	21.458605	4
CGCAGAG	9750	0.0	21.433544	8
GAGTACA	11275	0.0	20.430262	1
GTATAGA	1615	0.0	20.417715	1
GGACCGT	580	0.0	19.432192	6
GCAGAGT	11200	0.0	18.323227	9
AGTACAT	12200	0.0	18.225882	2
ATGGGGG	6400	0.0	18.057077	5
GTCTAGG	935	0.0	17.129665	1
>>END_MODULE