FastQCFastQC Report
Wed 25 May 2016
SRR1033189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086082
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60270.11849985902704675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104100.041.9026261
GGTATCA68400.040.3825951
TCAACGC123000.034.6677444
ATCAACG123350.034.6456033
CAACGCA124200.034.332795
TATCAAC126800.033.925422
AACGCAG128350.033.283516
ACGCAGA150550.028.1570327
CGCAGAG158750.026.6421078
GTACATG169000.026.1173061
TACATGG174850.024.6562542
ACATGGG167550.024.2152943
GCAGAGT178350.023.3978189
GAGTACA111250.020.9371431
GAGTACT93800.020.4907612-13
CATGGGG144900.020.4406914
CTAGTAC7200.020.241963
AGAGTAC165000.019.08210810-11
GTGGTAT30950.019.0047261
CAGAGTA179800.018.3869610-11
Produced by FastQC (version 0.11.5)