FastQCFastQC Report
Wed 25 May 2016
SRR1033188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989058
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36750.038.3204351
TCAACGC37350.035.9689334
ATCAACG38450.034.9457633
CAACGCA38650.034.8800665
AACGCAG41350.032.5938036
GGTATCA24050.032.509451
TATCAAC46700.029.0881862
GTACATG106950.028.3169671
TACATGG107150.027.2417562
ACGCAGA50800.026.3457037
ACATGGG110250.025.5680453
CGCAGAG54850.023.9723178
CATGGGG86850.021.7424454
GAGTACA69750.021.1357041
AGTACAT72400.018.892532
GCAGAGT68000.018.5768479
CGCCGTA2901.0927688E-616.28767894
CATGGGA30350.016.25114
GTGTAGG15300.016.0076831
AGAGTAC58300.015.5056810-11
Produced by FastQC (version 0.11.5)