##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033188_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2989058
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520240155928725	33.0	31.0	34.0	30.0	34.0
2	31.626837619075978	34.0	31.0	34.0	30.0	34.0
3	31.828597170078332	34.0	31.0	34.0	30.0	34.0
4	35.20571798874428	37.0	35.0	37.0	33.0	37.0
5	35.30116344346614	37.0	35.0	37.0	33.0	37.0
6	35.374255367410065	37.0	36.0	37.0	33.0	37.0
7	35.30629917519165	37.0	35.0	37.0	33.0	37.0
8	35.32229652285101	37.0	36.0	37.0	33.0	37.0
9	36.99497734737834	39.0	38.0	39.0	34.0	39.0
10-11	36.97040539193284	39.0	38.0	39.0	34.0	39.0
12-13	36.92031854182823	39.0	38.0	39.0	34.0	39.0
14-15	38.35170946833417	41.0	39.0	41.0	34.0	41.0
16-17	38.34263804850893	41.0	39.0	41.0	34.0	41.0
18-19	38.313648146004525	41.0	39.0	41.0	34.0	41.0
20-21	38.31679796778785	41.0	39.0	41.0	34.0	41.0
22-23	38.267072602806635	40.0	39.0	41.0	34.0	41.0
24-25	38.237373112197886	40.0	39.0	41.0	34.0	41.0
26-27	38.169896502510156	40.0	38.0	41.0	33.5	41.0
28-29	38.129913671798946	40.0	38.0	41.0	33.0	41.0
30-31	38.123938712463925	40.0	38.0	41.0	33.0	41.0
32-33	38.191459315945025	40.5	39.0	41.0	33.5	41.0
34-35	38.158306061642165	41.0	38.0	41.0	33.0	41.0
36-37	38.08629474570249	41.0	38.0	41.0	33.0	41.0
38-39	38.00442647817473	40.0	38.0	41.0	33.0	41.0
40-41	37.936921264157476	40.0	38.0	41.0	33.0	41.0
42-43	37.813207371687	40.0	38.0	41.0	33.0	41.0
44-45	37.68330574381628	40.0	38.0	41.0	32.5	41.0
46-47	37.54212815542556	40.0	38.0	41.0	32.0	41.0
48-49	37.40851917226096	40.0	37.0	41.0	32.0	41.0
50-51	37.258306463106436	40.0	37.0	41.0	31.5	41.0
52-53	37.08937581672888	40.0	37.0	41.0	31.0	41.0
54-55	36.91224526255429	40.0	36.0	41.0	31.0	41.0
56-57	36.725714756956876	40.0	36.0	41.0	31.0	41.0
58-59	36.50528326984622	39.0	35.0	41.0	31.0	41.0
60-61	36.25490974079459	39.0	35.0	41.0	30.0	41.0
62-63	35.967481059250105	39.0	35.0	41.0	30.0	41.0
64-65	35.67219655824678	38.0	35.0	40.0	30.0	41.0
66-67	35.35494644132031	37.0	35.0	40.0	29.0	41.0
68-69	35.02899910272735	37.0	34.5	40.0	29.0	41.0
70-71	34.67165775973567	36.0	34.0	39.0	29.0	41.0
72-73	34.30248660280262	36.0	34.0	39.0	29.0	41.0
74-75	33.93099080044616	35.0	34.0	38.5	28.0	40.0
76-77	33.61357223580138	35.0	34.0	37.0	28.0	39.5
78-79	33.26685330294695	35.0	34.0	37.0	27.5	39.0
80-81	32.933150176410095	35.0	33.5	36.5	27.0	39.0
82-83	32.74710042428083	35.0	34.0	36.0	27.0	37.5
84-85	32.509381383700145	35.0	33.0	36.0	27.0	37.0
86-87	32.291120312820965	35.0	33.0	35.0	27.0	37.0
88-89	31.96605134460422	35.0	33.0	35.0	26.0	36.0
90-91	31.805332482675144	35.0	33.0	35.0	26.0	36.0
92-93	31.64797605131784	35.0	33.0	35.0	25.0	36.0
94-95	31.489861187036183	35.0	33.0	35.0	25.0	36.0
96-97	31.323786624414783	35.0	33.0	35.0	24.5	35.0
98-99	31.138506847307745	35.0	33.0	35.0	24.0	35.0
100	30.97884450552649	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14409815946039473
1101	2	0.12915285503727247
1101	3	0.1275694044506288
1101	4	0.09962485508348351
1101	5	0.10690178447396192
1101	6	0.13196571201883955
1101	7	0.18750891883770038
1101	8	0.1306081227160334
1101	9	0.18322326137374034
1101	10-11	0.147642568818533
1101	12-13	0.21708904399608286
1101	14-15	0.24011013580523866
1101	16-17	0.21683838453628823
1101	18-19	0.2715119972681279
1101	20-21	0.26924141775730703
1101	22-23	0.23941021923046435
1101	24-25	0.30867993372596914
1101	26-27	0.3410225026106346
1101	28-29	0.3097379068384427
1101	30-31	0.29981423882210834
1101	32-33	0.2812166226842052
1101	34-35	0.3283488471356293
1101	36-37	0.36173256185141867
1101	38-39	0.3260644351202018
1101	40-41	0.2548131983628039
1101	42-43	0.23036890579675884
1101	44-45	0.16834890512278378
1101	46-47	0.23673935320933026
1101	48-49	0.22590595061979002
1101	50-51	0.13048604911791983
1101	52-53	0.12921572870653364
1101	54-55	0.11625324001586534
1101	56-57	0.1595164612289821
1101	58-59	0.1278698248030068
1101	60-61	0.0966806264176796
1101	62-63	0.12686079160032193
1101	64-65	0.060063175843318106
1101	66-67	0.14164773012468856
1101	68-69	0.1689368017918369
1101	70-71	0.1955486828241817
1101	72-73	0.24737224550406012
1101	74-75	0.15649164208980437
1101	76-77	0.09265050307936207
1101	78-79	0.13234552877202077
1101	80-81	0.10697010127926632
1101	82-83	0.07026136203442945
1101	84-85	0.09362670273843321
1101	86-87	0.18060290857930283
1101	88-89	0.3014657189370311
1101	90-91	0.31852481791624854
1101	92-93	0.3932124811772546
1101	94-95	0.2994251019788372
1101	96-97	0.31263665058600765
1101	98-99	0.35511219838092956
1101	100	0.3654748445145266
1102	1	0.19569084982160234
1102	2	0.20671506144344676
1102	3	0.24643457785478873
1102	4	0.19336822780133645
1102	5	0.14043521931243674
1102	6	0.14398575981226713
1102	7	0.13325502053228178
1102	8	0.1897713068844169
1102	9	0.21222357034171324
1102	10-11	0.23674144416244758
1102	12-13	0.24114935486619515
1102	14-15	0.284783085685703
1102	16-17	0.3070251362644143
1102	18-19	0.31656439464787667
1102	20-21	0.25020392708704975
1102	22-23	0.3065068494955909
1102	24-25	0.35547972750123336
1102	26-27	0.35698069521320264
1102	28-29	0.31198814759064675
1102	30-31	0.3093833925953504
1102	32-33	0.31388780792327964
1102	34-35	0.35485549565679975
1102	36-37	0.3145453901875541
1102	38-39	0.2308433569183208
1102	40-41	0.33949248217309247
1102	42-43	0.37318275902757847
1102	44-45	0.2730018115931614
1102	46-47	0.23620934366459778
1102	48-49	0.28971224794871375
1102	50-51	0.2759757310470121
1102	52-53	0.261847711868505
1102	54-55	0.2824131211626906
1102	56-57	0.24260003536257813
1102	58-59	0.23622939522852704
1102	60-61	0.1800215200554689
1102	62-63	0.22860849367630465
1102	64-65	0.26283160841830266
1102	66-67	0.18154101995988015
1102	68-69	0.21643086510241716
1102	70-71	0.2385836468435727
1102	72-73	0.23873184124915525
1102	74-75	0.2092729391981223
1102	76-77	0.1647233228552878
1102	78-79	0.19270798966582703
1102	80-81	0.20571495424033515
1102	82-83	0.16197195969336775
1102	84-85	0.13597896117199326
1102	86-87	0.24907665815445057
1102	88-89	0.26827504994265183
1102	90-91	0.2815336468330756
1102	92-93	0.29527055850368455
1102	94-95	0.33860534888443894
1102	96-97	0.2968328266157343
1102	98-99	0.33412129422023185
1102	100	0.30649426116431755
1103	1	0.035438969224482975
1103	2	-0.027594702397731652
1103	3	0.01883378476267339
1103	4	0.06032944952055175
1103	5	0.027839573283102936
1103	6	0.04381248135100435
1103	7	0.03175171007079314
1103	8	0.03445930464332747
1103	9	0.09923847145884679
1103	10-11	0.10867228631439474
1103	12-13	0.12030833535717989
1103	14-15	0.06520743441278398
1103	16-17	0.04332565514580722
1103	18-19	0.06641001643460953
1103	20-21	0.040558397646663025
1103	22-23	0.07759959359610491
1103	24-25	0.07063407877828354
1103	26-27	0.16175614477872813
1103	28-29	0.1748235259816866
1103	30-31	0.1250997456645564
1103	32-33	0.06164087820346964
1103	34-35	0.08656274361009508
1103	36-37	0.08784118041545241
1103	38-39	0.10244445483866826
1103	40-41	0.10705248484875796
1103	42-43	0.06258319355371356
1103	44-45	-0.027326776625656635
1103	46-47	0.09792406305203372
1103	48-49	0.055180484757592296
1103	50-51	0.08752628539443208
1103	52-53	-0.062084721540678345
1103	54-55	-0.010795971472767008
1103	56-57	-0.08086715901458064
1103	58-59	0.05838058688691916
1103	60-61	0.01934763867181033
1103	62-63	0.03300366553836653
1103	64-65	0.07127759501125297
1103	66-67	0.12198913316743187
1103	68-69	0.07210740980486463
1103	70-71	0.06055537593343274
1103	72-73	0.027519465537359622
1103	74-75	0.012280699298749198
1103	76-77	0.014742195044725293
1103	78-79	-0.030931242984109986
1103	80-81	0.046552278997779695
1103	82-83	0.04394103332083432
1103	84-85	-0.0026321635319277448
1103	86-87	0.0012676110412144226
1103	88-89	0.024580538556712384
1103	90-91	0.10154758969416378
1103	92-93	0.10639220965807894
1103	94-95	0.06208234692348569
1103	96-97	0.06741190260461138
1103	98-99	0.10999417927799016
1103	100	0.16766486889932253
1104	1	-0.0283730712525454
1104	2	-0.06503276810615688
1104	3	0.002643049248536755
1104	4	-0.024139887587494968
1104	5	-0.01558681029170117
1104	6	-0.0021969717548984136
1104	7	0.005658259936353716
1104	8	-0.007203100158164943
1104	9	0.057435858516527105
1104	10-11	0.016602129367960572
1104	12-13	-0.01793545582759748
1104	14-15	-0.03475956141434722
1104	16-17	0.060177991052434265
1104	18-19	0.03353613744962303
1104	20-21	-0.032595931209257856
1104	22-23	-0.015038438826429967
1104	24-25	7.499097726508808E-4
1104	26-27	-0.0033493699736837357
1104	28-29	0.11091115108548877
1104	30-31	0.07890891046611159
1104	32-33	0.10664530765384939
1104	34-35	0.053603401587174915
1104	36-37	0.12325251252715219
1104	38-39	0.06013041807509012
1104	40-41	0.07793388696483561
1104	42-43	0.07192174377119187
1104	44-45	0.057551103600616216
1104	46-47	-0.03480656308674668
1104	48-49	-0.113014132810072
1104	50-51	-0.06374058292347229
1104	52-53	-0.13864272762054952
1104	54-55	-0.15386215339921705
1104	56-57	-0.0735052564486196
1104	58-59	-0.14405477651004617
1104	60-61	-0.08590966818768209
1104	62-63	-0.0771900188975323
1104	64-65	-0.16227384469014794
1104	66-67	-0.15953467361144646
1104	68-69	-0.17423219879987784
1104	70-71	-0.11783299108164158
1104	72-73	-0.11627706644569713
1104	74-75	-0.16721901226687663
1104	76-77	-0.2510969095041773
1104	78-79	-0.2472217223962616
1104	80-81	-0.16798874087668025
1104	82-83	-0.16162360458243086
1104	84-85	-0.0728254351447859
1104	86-87	-0.0926869855754262
1104	88-89	-0.022375590381038535
1104	90-91	-0.13177081085817122
1104	92-93	-0.14122945286807465
1104	94-95	-0.13862390011849257
1104	96-97	-0.08393300660465997
1104	98-99	-0.09220776573614842
1104	100	-0.07837300506851008
1105	1	0.03105130168606962
1105	2	0.02120085987752418
1105	3	0.09973747060772098
1105	4	0.0598459036994754
1105	5	0.05885631350322029
1105	6	0.051006076752557306
1105	7	0.06421568250187448
1105	8	0.008950559955515303
1105	9	0.045255007363827815
1105	10-11	0.07143585283940723
1105	12-13	0.06629422399181806
1105	14-15	0.16761954485193087
1105	16-17	0.16594282987610143
1105	18-19	0.07730937760928924
1105	20-21	0.07774290102466352
1105	22-23	0.12938411604352495
1105	24-25	0.12316605409701253
1105	26-27	0.048433606095059645
1105	28-29	0.07943645095716789
1105	30-31	0.11352359060298767
1105	32-33	0.09626378056807994
1105	34-35	0.1361840795586602
1105	36-37	0.10698210337502445
1105	38-39	0.05742701994709165
1105	40-41	-0.0020918093105493085
1105	42-43	0.03297622776968723
1105	44-45	9.904498644743853E-4
1105	46-47	0.08976507863447125
1105	48-49	-0.04615726859316993
1105	50-51	-0.06338427906018751
1105	52-53	0.014449174340334991
1105	54-55	-0.011623447772208806
1105	56-57	0.027181495229733343
1105	58-59	0.018233514041760657
1105	60-61	0.03169848862277291
1105	62-63	9.275374802086844E-5
1105	64-65	-0.02221105187771144
1105	66-67	-0.004822461035466574
1105	68-69	-0.024375305134654468
1105	70-71	-0.020486222628193218
1105	72-73	-0.037846905553408305
1105	74-75	-0.033375220391782534
1105	76-77	-0.05367783601118248
1105	78-79	-0.05180033146618257
1105	80-81	-0.09916868101094423
1105	82-83	-0.08955336844445583
1105	84-85	-0.08400577418900212
1105	86-87	-0.011616160888621607
1105	88-89	-0.07513205815245172
1105	90-91	-0.10157826161889716
1105	92-93	-0.11402295869758916
1105	94-95	-0.04577901844646348
1105	96-97	-0.020216305143019753
1105	98-99	0.013890298652512456
1105	100	0.03387080493506289
1106	1	0.061149169533155856
1106	2	0.04897209192066754
1106	3	0.013671781923523696
1106	4	0.01170641691158636
1106	5	-0.016118978419974894
1106	6	0.13963891970264797
1106	7	0.00870548308731145
1106	8	0.028181398021665416
1106	9	0.05299683828700097
1106	10-11	0.06850160714666487
1106	12-13	0.004721249159253205
1106	14-15	0.0823517977910484
1106	16-17	0.045270297899776324
1106	18-19	0.059593114822739324
1106	20-21	0.026565290221128635
1106	22-23	0.02050842302423206
1106	24-25	0.01879115849733637
1106	26-27	0.08928870938178335
1106	28-29	0.04690493697349751
1106	30-31	0.03452212502129015
1106	32-33	0.024059273258060898
1106	34-35	0.05520944784323234
1106	36-37	-7.601252636746381E-4
1106	38-39	0.040287071809189
1106	40-41	0.040316919948587326
1106	42-43	0.061687112448090886
1106	44-45	0.08495383915638399
1106	46-47	0.04607582968046131
1106	48-49	0.05837855264960723
1106	50-51	0.16209695672148428
1106	52-53	0.0935342844677578
1106	54-55	0.09449754325532922
1106	56-57	0.12117141711839707
1106	58-59	0.14462365407739952
1106	60-61	0.11271463189581965
1106	62-63	0.13084732449365788
1106	64-65	0.202087949657475
1106	66-67	0.19187799156479457
1106	68-69	0.12672358285274044
1106	70-71	0.16770818867329496
1106	72-73	0.16063319972189305
1106	74-75	0.20521998872704472
1106	76-77	0.07858246084368403
1106	78-79	0.17002689295673434
1106	80-81	0.2317785083334627
1106	82-83	0.14203533603701146
1106	84-85	0.12808557311209512
1106	86-87	0.125234369663886
1106	88-89	0.25158120088762104
1106	90-91	0.24620027347551598
1106	92-93	0.22290612067051896
1106	94-95	0.1728453531630123
1106	96-97	0.08321063550987162
1106	98-99	0.15258744723107753
1106	100	0.23713997681180388
1107	1	0.11537783186297901
1107	2	0.09099829393960235
1107	3	0.06331607230665526
1107	4	0.1124382294293298
1107	5	0.0807588863936175
1107	6	0.10318214269363324
1107	7	0.07694236879348182
1107	8	0.027272508608319868
1107	9	0.09513764481744857
1107	10-11	0.11166521346437008
1107	12-13	0.0984910051531358
1107	14-15	0.07407698344891855
1107	16-17	0.03851830177546134
1107	18-19	0.11217214717576951
1107	20-21	0.1000168487515225
1107	22-23	0.07045767917201573
1107	24-25	0.04210812614047654
1107	26-27	0.16888017828704704
1107	28-29	0.1583185697402314
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2216	20-21	-0.23671150919408745
2216	22-23	-0.15152821623856028
2216	24-25	-0.16393915177872742
2216	26-27	-0.24329688265419946
2216	28-29	-0.16592660516011648
2216	30-31	-0.19497430088593148
2216	32-33	-0.1642338862269881
2216	34-35	-0.1881632550104868
2216	36-37	-0.2252914573805853
2216	38-39	-0.19056277253208975
2216	40-41	-0.14005571685705576
2216	42-43	-0.2348558376483325
2216	44-45	-0.22367945580422344
2216	46-47	-0.09633459716440029
2216	48-49	-0.04941846416509321
2216	50-51	-0.06326190115721886
2216	52-53	-0.09881019559401949
2216	54-55	-0.07344259529977393
2216	56-57	-0.09470990541546342
2216	58-59	-0.13400748115139294
2216	60-61	-0.05468676905292824
2216	62-63	-0.0882654714513933
2216	64-65	0.007418820167167439
2216	66-67	-0.10968076347339206
2216	68-69	-0.0948910577788169
2216	70-71	-0.05689364876008085
2216	72-73	0.05817598814233094
2216	74-75	0.15449943654885345
2216	76-77	0.08125082368997738
2216	78-79	0.02038557862417889
2216	80-81	0.03631745016211596
2216	82-83	0.04546130687293015
2216	84-85	0.010907317437933273
2216	86-87	-0.03992457727842691
2216	88-89	-0.04170132404552973
2216	90-91	-0.08760473637293842
2216	92-93	-0.0025647758288904754
2216	94-95	0.017596434953638607
2216	96-97	-0.014547173865636864
2216	98-99	-0.08793250516567497
2216	100	-0.04184024077408566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34260.0
3	5828.0
4	3995.0
5	3309.0
6	2999.0
7	3030.0
8	3569.0
9	3917.0
10	4369.0
11	4751.0
12	5187.0
13	5621.0
14	6176.0
15	6404.0
16	6464.0
17	6518.0
18	6518.0
19	6497.0
20	6683.0
21	6856.0
22	7937.0
23	10330.0
24	14854.0
25	19768.0
26	20450.0
27	19723.0
28	22633.0
29	27913.0
30	34364.0
31	42632.0
32	56026.0
33	75518.0
34	106622.0
35	166027.0
36	322139.0
37	663035.0
38	924303.0
39	321333.0
40	500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42839655392902	18.402014985514942	13.02258903754166	24.146999423014375
2	15.601821133549459	19.0175350924584	40.21572789239597	25.164915881596173
3	18.95798155039362	24.410410677150633	28.65979236493209	27.97181540752366
4	12.747818182548407	15.803199329557183	37.253018532228246	34.195963955666166
5	14.004363111558563	37.57145783351898	34.63987929483335	13.78429976008911
6	32.85321530966324	36.252228752945605	17.355783080649324	13.538772856741835
7	29.366835092199096	31.417049588839618	21.263505994597267	17.952609324364023
8	26.21346196443951	36.26754083352796	19.355898652143917	18.16309854988861
9	25.695753852825526	17.073227860871647	19.907204935150197	37.32381335115263
10-11	25.244262751212464	26.26807741786954	27.655259958275767	20.83239987264222
12-13	25.557498138745053	23.27952284951858	27.9855951414972	23.17738387023916
14-15	22.650790559595464	25.17794785104045	24.891384218789526	27.279877370574557
16-17	22.964422142568417	28.589139390889308	25.44765111989742	22.998787346644857
18-19	22.933173342283194	27.76213355159218	26.987263344945067	22.31742976117956
20-21	23.761982962212517	26.920306556360813	26.816152351249812	22.50155813017686
22-23	23.506448877762097	26.596927907616386	26.708786976566145	23.18783623805537
24-25	23.313873691301286	26.71089773975123	26.74919683173313	23.226031737214353
26-27	23.149551996182673	26.90621215675265	26.65834359217515	23.28589225488953
28-29	23.128643356989674	26.91023919461587	26.81037260660662	23.150744841787834
30-31	23.208180676516342	27.153159640577062	26.53034659692276	23.108313085983838
32-33	23.158458156332387	26.888516217159975	26.841315965112972	23.11170966139466
34-35	23.208183360709576	26.82390523191286	26.830664247251473	23.137247160126094
36-37	23.41387504257616	26.879072338312653	26.51427246465259	23.192780154458603
38-39	23.145111518911378	27.13676196734761	26.630833481253408	23.0872930324876
40-41	23.138463741581816	26.921226404993632	26.776852535894392	23.163457317530167
42-43	23.081901909357143	26.86446465094601	26.775248451974313	23.278384987722525
44-45	23.035784727526767	27.045903876118388	26.812598681271915	23.105712715082934
46-47	23.177445360415657	26.989694571055466	26.77740293554665	23.055457132982223
48-49	23.266604927780225	26.923663703289336	26.588426902987422	23.221304465943017
50-51	23.083112736110404	26.99969486653087	26.69344056722706	23.22375183013166
52-53	23.157203745532133	26.95618468831229	26.77199958110853	23.114611985047045
54-55	23.135863112115697	27.13202297657678	26.733405666257493	22.99870824505003
56-57	22.927713491654846	27.047562363226803	26.799752156336893	23.224971988781462
58-59	22.971861457016168	26.7342672421364	26.99514232036129	23.298728980486143
60-61	23.16029391469448	27.008334831902374	26.697725381298692	23.13364587210445
62-63	23.01661940966385	27.099239705919253	26.686288777925544	23.197852106491357
64-65	23.071572671705155	26.943415668837968	26.71539550149047	23.269616157966404
66-67	22.914431391048193	26.91715135973342	26.77354169698612	23.394875552232264
68-69	23.05382259784292	26.98140717690399	26.787425948050636	23.177344277202454
70-71	23.04847495455384	26.798972825647844	26.79624613977393	23.356306080024382
72-73	22.977986871146065	26.948342733947833	26.754497925552755	23.31917246935335
74-75	22.991065528933735	27.06962396934076	26.703111591393064	23.236198910332437
76-77	23.018701966618902	26.876260690673643	26.720569888224478	23.384467454482984
78-79	23.125431276394114	26.95592679362886	26.76589334540001	23.15274858457701
80-81	23.09682334242852	26.815060237839894	26.847011326912	23.241105092819588
82-83	22.98386570647128	26.839819670622873	26.914878846427285	23.26143577647856
84-85	23.01480333675203	26.821134279561633	26.717034334246303	23.44702804944003
86-87	23.066869478362094	26.921546300334278	26.799548140092632	23.212036081210996
88-89	22.922893242946486	26.84008107555525	26.817279627368805	23.419746054129458
90-91	22.921545884223615	26.82772488382441	26.860256740809458	23.390472491142518
92-93	23.101672332772193	26.851837048403592	26.835630544900347	23.210860073923868
94-95	22.968427393775	26.640326714130186	26.95209841808841	23.439147474006404
96-97	23.00006663359666	26.668363916359354	26.912939504097967	23.41862994594602
98-99	22.879576923271074	26.846611129152482	26.911391808693395	23.362420138883046
100	23.076384628509295	26.511416284228073	26.813048355023266	23.599150732239362
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	174.5
2	159.0
3	126.5
4	96.5
5	80.5
6	96.0
7	104.0
8	102.5
9	122.0
10	113.0
11	99.0
12	117.5
13	133.0
14	150.0
15	181.5
16	224.5
17	315.0
18	429.5
19	604.5
20	901.0
21	1323.0
22	2054.0
23	3395.0
24	5103.0
25	7321.5
26	10558.5
27	14717.0
28	20211.0
29	26352.5
30	32225.5
31	38705.0
32	45529.0
33	52382.5
34	59848.5
35	66940.5
36	73219.5
37	79917.5
38	85974.5
39	90031.0
40	94324.0
41	99220.0
42	102425.0
43	104742.0
44	106270.5
45	106981.0
46	107734.5
47	105739.0
48	104156.0
49	104985.5
50	104786.5
51	104885.5
52	101424.5
53	98888.5
54	98501.0
55	94282.5
56	87931.0
57	80055.0
58	71846.5
59	64925.5
60	58982.5
61	51482.5
62	44539.0
63	37994.0
64	31010.0
65	25334.0
66	19846.5
67	14509.5
68	10963.5
69	8672.5
70	6318.5
71	4408.0
72	3286.0
73	2456.5
74	1755.0
75	1116.0
76	672.5
77	429.5
78	271.0
79	194.0
80	127.5
81	76.5
82	52.0
83	41.5
84	37.5
85	25.5
86	18.0
87	20.5
88	16.0
89	9.5
90	7.0
91	7.5
92	7.0
93	8.5
94	7.5
95	5.5
96	5.0
97	2.5
98	2.0
99	2.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32722683868964736
2	0.11525370200243688
3	0.06336444458421349
4	0.03954423099183756
5	0.042889766608744294
6	0.009334044371169779
7	0.006691071233813462
8	0.015389463837770963
9	0.017965526262789147
10-11	0.02095978063992067
12-13	0.01615893702965951
14-15	0.01813280304363448
16-17	0.019069553016368368
18-19	0.018651361064255027
20-21	0.023435476996431653
22-23	0.015924749536476043
24-25	0.02463986981851807
26-27	0.019370651221889974
28-29	0.019103008372537433
30-31	0.020106669057609453
32-33	0.024305316256827404
34-35	0.01528909776926376
36-37	0.009902785426043925
38-39	0.013198138008697053
40-41	0.009702053289029521
42-43	0.008397294398435895
44-45	0.008999490809479108
46-47	0.008196562261421491
48-49	0.0042153748773024816
50-51	0.006757981946151598
52-53	0.007259812288687606
54-55	0.003378990973075799
56-57	0.002576062425018183
58-59	0.002659700815440851
60-61	0.003194986514145928
62-63	0.0027767945620325867
64-65	0.002274964219496577
66-67	0.0017731338769605674
68-69	0.003646633822428337
70-71	0.002726611527778986
72-73	0.0023084195756656444
74-75	0.0024422410003419135
76-77	0.004048098096457144
78-79	0.003696816856681938
80-81	0.003830638281358207
82-83	0.0027767945620325867
84-85	0.0034626293634984666
86-87	0.002626245459271784
88-89	0.5940165764598746
90-91	0.6063448752081759
92-93	0.6031498886940301
94-95	0.5905874024525453
96-97	0.5882789828768796
98-99	0.5852345454654945
100	0.5873756882603147
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2989058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.94191945324755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85685726324571	46.479745291464674
2	11.991379973515528	14.135899050644756
3	3.658555984082676	6.469269363869937
4	1.6353628405973657	3.855656993092961
5	0.9355074084522007	2.757030115845299
6	0.5942702720354065	2.1016458304662264
7	0.41879289654741014	1.7279120023122776
8	0.29340026322104745	1.3834859745869283
9	0.21905742945802642	1.1620498826475403
>10	1.2844567146343087	13.87840932486836
>50	0.08378092932631526	3.370054422461509
>100	0.028350616153648287	2.5967428169612674
>500	2.274087303475187E-4	0.08209893077821838
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.345535616906731E-5	0.0
16-17	0.0	0.0	3.345535616906731E-5	3.345535616906731E-5	0.0
18-19	0.0	0.0	3.345535616906731E-5	8.363839042266829E-5	0.0
20-21	0.0	0.0	3.345535616906731E-5	1.6727678084533658E-4	0.0
22-23	0.0	0.0	3.345535616906731E-5	2.8437052743707214E-4	0.0
24-25	0.0	0.0	3.345535616906731E-5	5.35285698705077E-4	0.0
26-27	3.345535616906731E-5	0.0	3.345535616906731E-5	7.694731918885481E-4	0.0
28-29	1.0036606850720194E-4	0.0	3.345535616906731E-5	0.00155567406186163	0.0
30-31	1.1709374659173559E-4	0.0	3.345535616906731E-5	0.004365923980063285	0.0
32-33	1.3382142467626925E-4	0.0	3.345535616906731E-5	0.01112390592621488	0.0
34-35	1.3382142467626925E-4	3.345535616906731E-5	3.345535616906731E-5	0.019504472646566243	0.0
36-37	1.3382142467626925E-4	3.345535616906731E-5	3.345535616906731E-5	0.03649979358045244	0.0
38-39	1.3382142467626925E-4	3.345535616906731E-5	3.345535616906731E-5	0.06662634181069756	0.0
40-41	1.3382142467626925E-4	3.345535616906731E-5	3.345535616906731E-5	0.10235666219926144	0.0
42-43	1.3382142467626925E-4	3.345535616906731E-5	5.018303425360097E-5	0.14211835300619793	0.0
44-45	1.3382142467626925E-4	3.345535616906731E-5	6.691071233813463E-5	0.18455647230665984	0.0
46-47	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.2364290020467987	0.0
48-49	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.29531042890435716	0.0
50-51	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.36098329306423627	0.0
52-53	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.4273921750598349	0.0
54-55	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.4976818783710454	0.0
56-57	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.5796642286633448	0.0
58-59	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.6753130919507082	0.0
60-61	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.7754784283208958	0.0
62-63	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.882970487692109	0.0
64-65	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	0.995815404050373	0.0
66-67	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	1.1177936326427926	0.0
68-69	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	1.2602967222449348	0.0
70-71	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	1.418005271225918	0.0
72-73	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	1.5826892619681519	0.0
74-75	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	1.7502504133409253	0.0
76-77	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	1.9310431580785652	0.0
78-79	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	2.1403398662722504	0.0
80-81	1.3382142467626925E-4	1.0036606850720194E-4	6.691071233813463E-5	2.3682544801740213	0.0
82-83	1.3382142467626925E-4	1.0036606850720194E-4	8.363839042266829E-5	2.6086144865706853	0.0
84-85	1.3382142467626925E-4	1.0036606850720194E-4	1.0036606850720194E-4	2.8484392072686444	0.0
86-87	1.3382142467626925E-4	1.0036606850720194E-4	1.0036606850720194E-4	3.0974306955569277	0.0
88	1.3382142467626925E-4	1.0036606850720194E-4	1.3382142467626925E-4	3.299601412886602	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3675	0.0	38.320435	1
TCAACGC	3735	0.0	35.968933	4
ATCAACG	3845	0.0	34.945763	3
CAACGCA	3865	0.0	34.880066	5
AACGCAG	4135	0.0	32.593803	6
GGTATCA	2405	0.0	32.50945	1
TATCAAC	4670	0.0	29.088186	2
GTACATG	10695	0.0	28.316967	1
TACATGG	10715	0.0	27.241756	2
ACGCAGA	5080	0.0	26.345703	7
ACATGGG	11025	0.0	25.568045	3
CGCAGAG	5485	0.0	23.972317	8
CATGGGG	8685	0.0	21.742445	4
GAGTACA	6975	0.0	21.135704	1
AGTACAT	7240	0.0	18.89253	2
GCAGAGT	6800	0.0	18.576847	9
CGCCGTA	290	1.0927688E-6	16.287678	94
CATGGGA	3035	0.0	16.2511	4
GTGTAGG	1530	0.0	16.007683	1
AGAGTAC	5830	0.0	15.50568	10-11
>>END_MODULE