FastQCFastQC Report
Wed 25 May 2016
SRR1033188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989058
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34260.1146180502352246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.044.8018841
GTATCAA61150.040.4033551
TCAACGC70450.034.031864
ATCAACG72500.033.069583
CAACGCA72650.032.936595
AACGCAG74850.031.8961016
TATCAAC77900.031.1996362
GTGGTAT17600.028.343131
GTACATG100100.027.549781
ACGCAGA88800.026.8324747
CGCAGAG92100.025.665658
TACATGG102450.025.6505322
ACATGGG105100.023.8855383
GCAGAGT103800.022.5916069
GTATAGG8950.022.0841561
CATGGGG84850.021.053694
TGGTATC22550.020.6388342
GAGTACA65350.020.0915371
CAGAGTA100650.019.30659510-11
AGAGTAC93700.018.9330810-11
Produced by FastQC (version 0.11.5)