FastQCFastQC Report
Wed 25 May 2016
SRR1033187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496970
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17150.034.2408644
GTATCAA18600.033.421091
ATCAACG17650.033.27423
CAACGCA17800.033.2533045
GGTATCA12050.031.6561131
AACGCAG19650.030.1135316
TATCAAC21700.027.7246952
GTACATG35200.026.8913731
CGACGCG459.250803E-426.24033590-91
TACATGG35750.025.3745172
ACGCAGA23650.024.424617
ACATGGG36050.023.8499873
CTGTCGC4000.023.4813849
CGCAGAG24400.023.4813848
TACACCG1703.4677523E-622.1067715
TCTGTCG4250.020.995128
GTACCGT1150.003977400720.4185946
ACACCGT2551.3593308E-820.2584486
TGTACCG1456.623061E-419.4387135
CACCGTT2702.6208E-819.132987
Produced by FastQC (version 0.11.5)