FastQCFastQC Report
Wed 25 May 2016
SRR1033187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496970
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.19385826035257886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24270.162127497545041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23000.052.1669771
GTATCAA42000.043.355581
TCAACGC48350.036.8509454
ATCAACG49100.036.479543
CAACGCA50400.035.4453285
TATCAAC52200.034.5833132
AACGCAG52400.034.171896
TAGACCG600.00415631631.341135
ACGCAGA58400.030.5001327
CGCAGAG58550.030.2614528
TCTGTCG4350.027.0100948
CGACCGT350.00883904326.85664620-21
GTACATG37600.026.7799191
GCAGAGT66700.026.4229169
ACATGGG36500.024.8582383
TACATGG38250.024.8270912
GTGGTAT10950.023.2040081
GAGTACT47650.022.83302312-13
CTGTCGC4400.022.4306569
CAGAGTA65700.021.63890610-11
Produced by FastQC (version 0.11.5)