FastQCFastQC Report
Wed 25 May 2016
SRR1033186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161910
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.040.0103571
GTATCAA43250.036.8157961
TCAACGC45300.034.1206784
ATCAACG46750.032.9676323
CAACGCA48900.031.3205015
AACGCAG50150.030.7135816
GTACATG103100.028.1008871
TATCAAC59300.026.7188822
TACATGG101800.026.6945842
ACGCAGA57950.026.0937737
CGCAGAG61900.024.353188
ACATGGG108050.024.0924263
TATACCG2304.123649E-922.4690555
CATGGGG74600.021.2232074
GCAGAGT73250.019.7462379
GAGTACA73400.018.9976221
AGTACAT73350.017.819182
GTGTATA16600.017.5949151
AGAGTAC62700.017.03943410-11
GTATAGA11650.016.983491
Produced by FastQC (version 0.11.5)