##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033186_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3161910
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.672148479874505	33.0	31.0	34.0	30.0	34.0
2	31.83924273619426	34.0	31.0	34.0	30.0	34.0
3	31.962595393290766	34.0	31.0	34.0	30.0	34.0
4	35.336464984771865	37.0	35.0	37.0	33.0	37.0
5	35.39903634195787	37.0	35.0	37.0	33.0	37.0
6	35.519208959141785	37.0	36.0	37.0	35.0	37.0
7	35.46429183626353	37.0	37.0	37.0	35.0	37.0
8	35.47297582790149	37.0	37.0	37.0	35.0	37.0
9	37.14792198386419	39.0	38.0	39.0	35.0	39.0
10-11	37.13153125800545	39.0	38.0	39.0	35.0	39.0
12-13	37.08108738072873	39.0	38.0	39.0	35.0	39.0
14-15	38.542941608078664	41.0	39.0	41.0	35.0	41.0
16-17	38.54265997450908	41.0	39.0	41.0	35.0	41.0
18-19	38.53070881207877	41.0	39.0	41.0	35.0	41.0
20-21	38.55212150250956	41.0	39.0	41.0	34.5	41.0
22-23	38.508484428715555	41.0	39.0	41.0	34.0	41.0
24-25	38.496254953493306	41.0	39.0	41.0	34.0	41.0
26-27	38.44791107273768	40.5	39.0	41.0	34.0	41.0
28-29	38.41583916050742	40.0	39.0	41.0	34.0	41.0
30-31	38.40983171564023	40.0	39.0	41.0	34.0	41.0
32-33	38.476665211849806	41.0	39.0	41.0	34.0	41.0
34-35	38.44480361553617	41.0	39.0	41.0	34.0	41.0
36-37	38.36282057364062	41.0	39.0	41.0	34.0	41.0
38-39	38.282268628771845	41.0	38.5	41.0	34.0	41.0
40-41	38.21601737557363	41.0	38.0	41.0	33.0	41.0
42-43	38.09701398838044	40.0	38.0	41.0	33.0	41.0
44-45	37.96288430094468	40.0	38.0	41.0	33.0	41.0
46-47	37.82843249807869	40.0	38.0	41.0	33.0	41.0
48-49	37.701218092861595	40.0	38.0	41.0	33.0	41.0
50-51	37.56514290413073	40.0	37.0	41.0	32.0	41.0
52-53	37.40510197949973	40.0	37.0	41.0	32.0	41.0
54-55	37.23846298597999	40.0	36.5	41.0	32.0	41.0
56-57	37.067426175950615	40.0	36.0	41.0	31.0	41.0
58-59	36.85643123934584	39.5	35.5	41.0	31.0	41.0
60-61	36.618121009136885	39.0	35.0	41.0	31.0	41.0
62-63	36.33882906218077	39.0	35.0	41.0	31.0	41.0
64-65	36.06011034469672	38.5	35.0	40.5	30.5	41.0
66-67	35.75570936554171	37.5	35.0	40.0	30.0	41.0
68-69	35.43952199777982	37.0	35.0	40.0	30.0	41.0
70-71	35.09213813802417	36.5	35.0	39.5	29.5	41.0
72-73	34.72404590896009	36.0	34.0	39.0	29.0	41.0
74-75	34.35126932771648	35.5	34.0	39.0	29.0	40.5
76-77	34.03015392594982	35.0	34.0	37.5	29.0	40.0
78-79	33.671617471718044	35.0	34.0	37.0	29.0	39.0
80-81	33.326335980467505	35.0	34.0	37.0	28.0	39.0
82-83	33.12465187181166	35.0	34.0	36.0	28.5	38.5
84-85	32.874387316527034	35.0	34.0	36.0	28.0	37.0
86-87	32.64346312829903	35.0	34.0	35.5	28.0	37.0
88-89	32.30372622876679	35.0	34.0	35.0	27.0	36.5
90-91	32.13655527829698	35.0	34.0	35.0	27.0	36.0
92-93	31.977505368590506	35.0	33.0	35.0	27.0	36.0
94-95	31.822893282857514	35.0	33.0	35.0	26.0	36.0
96-97	31.659325217985334	35.0	33.0	35.0	25.5	35.5
98-99	31.47712537675013	35.0	33.0	35.0	25.0	35.0
100	31.318051430938894	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24251199942298385
1101	2	0.22999639011239026
1101	3	0.24250721964610378
1101	4	0.2419506413818766
1101	5	0.19519086820768905
1101	6	0.20157340141999924
1101	7	0.24432916249902803
1101	8	0.09969996740570508
1101	9	0.2526169491919745
1101	10-11	0.2256900323915545
1101	12-13	0.24930288413195711
1101	14-15	0.25974967670212123
1101	16-17	0.18864057105184884
1101	18-19	0.26208771509868
1101	20-21	0.2015988642806903
1101	22-23	0.24243088453201267
1101	24-25	0.260726317116152
1101	26-27	0.26875757330322614
1101	28-29	0.3383993696646499
1101	30-31	0.30479376026832483
1101	32-33	0.290582247736225
1101	34-35	0.34791653921971744
1101	36-37	0.319817133956505
1101	38-39	0.33011319658692173
1101	40-41	0.3317412107928419
1101	42-43	0.3628086563848143
1101	44-45	0.3180373660849156
1101	46-47	0.3375115570157732
1101	48-49	0.26578382398798084
1101	50-51	0.20469834058882697
1101	52-53	0.26753680376695854
1101	54-55	0.19809344777746674
1101	56-57	0.20945249743459726
1101	58-59	0.20483743915499275
1101	60-61	0.25325966291760693
1101	62-63	0.2662151764943559
1101	64-65	0.20121897440437664
1101	66-67	0.11812361883334432
1101	68-69	0.14124957639546665
1101	70-71	0.23870346093376327
1101	72-73	0.27333594493045155
1101	74-75	0.1914704340934037
1101	76-77	0.14532148341794482
1101	78-79	0.1182905987294589
1101	80-81	0.20818583662359202
1101	82-83	0.11470064538665525
1101	84-85	0.05608981586109962
1101	86-87	0.1299487182827619
1101	88-89	0.3299979782239433
1101	90-91	0.36062176539721946
1101	92-93	0.34392484593812966
1101	94-95	0.373395205209043
1101	96-97	0.38574026272239337
1101	98-99	0.418846936659115
1101	100	0.4119490287698113
1102	1	0.12614660839500402
1102	2	0.15987455612254564
1102	3	0.18407317218959562
1102	4	0.1900514292559663
1102	5	0.06908783502797178
1102	6	0.05637613032991595
1102	7	0.14977765315819624
1102	8	0.17062675796130122
1102	9	0.22591470558828775
1102	10-11	0.20711662092006833
1102	12-13	0.22309412554895403
1102	14-15	0.21947017334203878
1102	16-17	0.2468487169349416
1102	18-19	0.21126380076572104
1102	20-21	0.24412280653813667
1102	22-23	0.26093708496682666
1102	24-25	0.2976546450946671
1102	26-27	0.32827085823830515
1102	28-29	0.35261123398061756
1102	30-31	0.330646765239905
1102	32-33	0.3288439229157589
1102	34-35	0.33514581190332393
1102	36-37	0.3335537145993541
1102	38-39	0.23100599238324548
1102	40-41	0.3416642545498334
1102	42-43	0.34677413591388984
1102	44-45	0.2988538548984181
1102	46-47	0.2523947280025354
1102	48-49	0.21379746601616745
1102	50-51	0.3062427919040758
1102	52-53	0.23546295640866077
1102	54-55	0.24748843813627275
1102	56-57	0.19997455935889263
1102	58-59	0.1882208695306602
1102	60-61	0.16303511029254736
1102	62-63	0.23691124330133562
1102	64-65	0.258314947037114
1102	66-67	0.18734523465915487
1102	68-69	0.2574755813462275
1102	70-71	0.37460906228422886
1102	72-73	0.36924499114201836
1102	74-75	0.23767987345407704
1102	76-77	0.19964352340606695
1102	78-79	0.28057735930562444
1102	80-81	0.2797502258998392
1102	82-83	0.28825285819775814
1102	84-85	0.20147020353876854
1102	86-87	0.22902724157513177
1102	88-89	0.3460741017750095
1102	90-91	0.3072615602562152
1102	92-93	0.3294202629806371
1102	94-95	0.26534341756881474
1102	96-97	0.36077045647297723
1102	98-99	0.37092286732316637
1102	100	0.37412079780211727
1103	1	0.10803839087100542
1103	2	0.10843987154042978
1103	3	0.08539248223650375
1103	4	0.10818733304929395
1103	5	0.05091392041970266
1103	6	0.08969802322225462
1103	7	0.07940009146199856
1103	8	0.012936816230144643
1103	9	0.09043585728481673
1103	10-11	0.13649899091901574
1103	12-13	0.09262997037073717
1103	14-15	0.125051675978618
1103	16-17	0.09914186687539228
1103	18-19	0.19634890387395387
1103	20-21	0.12322665005381594
1103	22-23	0.1640335086829623
1103	24-25	0.11357377746288222
1103	26-27	0.14837545282195208
1103	28-29	0.14075874610190198
1103	30-31	0.13489413451652865
1103	32-33	0.11012531061511766
1103	34-35	0.1210600755495932
1103	36-37	0.08667686451266121
1103	38-39	0.09471487779576648
1103	40-41	0.16448298820158413
1103	42-43	0.11591704481591592
1103	44-45	0.042625726071008785
1103	46-47	0.07586414930220542
1103	48-49	0.07247398995981058
1103	50-51	0.026241391505635647
1103	52-53	0.029949784771588384
1103	54-55	0.022803253839114745
1103	56-57	0.06299546007948464
1103	58-59	0.07806364791313314
1103	60-61	0.0364926488143098
1103	62-63	0.0920786532448119
1103	64-65	0.0803789433359654
1103	66-67	0.11982300798339907
1103	68-69	0.061884303063934
1103	70-71	0.04586117934385925
1103	72-73	0.13063556870233128
1103	74-75	0.10436641512236378
1103	76-77	0.06555218043200739
1103	78-79	0.03700974222784481
1103	80-81	0.11326836055704348
1103	82-83	0.10766049568738367
1103	84-85	0.12303117731934776
1103	86-87	0.09591205573683226
1103	88-89	0.16162403361993682
1103	90-91	0.2545808930231601
1103	92-93	0.20160756571974403
1103	94-95	0.20018533088103396
1103	96-97	0.16748438181193492
1103	98-99	0.20349593409221356
1103	100	0.2096273122686938
1104	1	0.061252775803723125
1104	2	0.10275615160124829
1104	3	0.1133958643583064
1104	4	0.11374453306814303
1104	5	0.0025410246540218395
1104	6	0.033176228557259435
1104	7	0.021172334123619407
1104	8	0.05805643246885239
1104	9	0.08079779715113489
1104	10-11	0.07620743483430914
1104	12-13	0.0432552690714445
1104	14-15	-0.015086166385977151
1104	16-17	0.007504286888426748
1104	18-19	0.059911466789195345
1104	20-21	0.029793793522379985
1104	22-23	0.0155139997950684
1104	24-25	0.035658504490953646
1104	26-27	0.09007846029964384
1104	28-29	0.103811978039964
1104	30-31	0.09363281945057622
1104	32-33	0.0971361417847092
1104	34-35	0.04634476472328686
1104	36-37	0.11886413702475807
1104	38-39	0.022369118158067636
1104	40-41	0.01792516704976066
1104	42-43	0.06353058882418594
1104	44-45	0.003339282939315069
1104	46-47	-0.08315411804547068
1104	48-49	-0.1465788746592267
1104	50-51	-0.08656451938892218
1104	52-53	-0.12236493466722465
1104	54-55	-0.12388936061980615
1104	56-57	-0.11716752757497773
1104	58-59	-0.14440893161565072
1104	60-61	-0.0790910395892368
1104	62-63	-0.06662723327470843
1104	64-65	-0.14259037807504882
1104	66-67	-0.1155533938597344
1104	68-69	-0.1497838623275527
1104	70-71	-0.17370954606572298
1104	72-73	-0.15340743413646152
1104	74-75	-0.17700909783970786
1104	76-77	-0.18474087163585295
1104	78-79	-0.18255145185313637
1104	80-81	-0.17125851445123885
1104	82-83	-0.1436742289941435
1104	84-85	-0.15035038131124168
1104	86-87	-0.11301033708918595
1104	88-89	-0.078717996897538
1104	90-91	-0.05236809385467467
1104	92-93	-0.06083514833430215
1104	94-95	0.012729546327378216
1104	96-97	0.018049333171678228
1104	98-99	-0.021277275926689043
1104	100	-0.02563079465848972
1105	1	0.07703105083265882
1105	2	0.08086135548482076
1105	3	0.09927648196248029
1105	4	0.06122372619631733
1105	5	0.040003396244301825
1105	6	0.06102862554034516
1105	7	-0.038018888492992176
1105	8	0.01278310411093031
1105	9	-0.020418286803405294
1105	10-11	0.054444994698052085
1105	12-13	0.10614031680938751
1105	14-15	0.15481997298686423
1105	16-17	0.15451419083753848
1105	18-19	0.12751927364269022
1105	20-21	0.12102590999638352
1105	22-23	0.14788979847850214
1105	24-25	0.14600224297377196
1105	26-27	0.1773137518284571
1105	28-29	0.17612951776141017
1105	30-31	0.1761169732529666
1105	32-33	0.2000501738540308
1105	34-35	0.1988700516938522
1105	36-37	0.13016619793530992
1105	38-39	0.14521286710841252
1105	40-41	0.1170698682839344
1105	42-43	0.10973122318384299
1105	44-45	0.13782889269247534
1105	46-47	0.17711849712540584
1105	48-49	0.09705796621148721
1105	50-51	0.19273595436639823
1105	52-53	0.15672784022218877
1105	54-55	0.1754177546608986
1105	56-57	0.2163425130101615
1105	58-59	0.1385921595186801
1105	60-61	0.16857140027718032
1105	62-63	0.19126590515867292
1105	64-65	0.20925792085053985
1105	66-67	0.24189258605881747
1105	68-69	0.14581837878252202
1105	70-71	0.17382590184319469
1105	72-73	0.16859121839127056
1105	74-75	0.2153266395496516
1105	76-77	0.19052596872031557
1105	78-79	0.1999947348012654
1105	80-81	0.15665033270630602
1105	82-83	0.13549002742902871
1105	84-85	0.17596147115050087
1105	86-87	0.16974781452721288
1105	88-89	0.19947915368523184
1105	90-91	0.13770607294698323
1105	92-93	0.17211039981065923
1105	94-95	0.21208672892122138
1105	96-97	0.3176863614342622
1105	98-99	0.3354426069933929
1105	100	0.3546085831111583
1106	1	0.1576610588637699
1106	2	0.15506491456383031
1106	3	0.1723794267482468
1106	4	0.16485296051460097
1106	5	0.08214629124875472
1106	6	0.14440382191953205
1106	7	0.10580675396258954
1106	8	0.1667071020815456
1106	9	0.15393186346258148
1106	10-11	0.11889059627203125
1106	12-13	0.13047013317174105
1106	14-15	0.14287281351962378
1106	16-17	0.13263067893852565
1106	18-19	0.1484769581878993
1106	20-21	0.13676270331527718
1106	22-23	0.14954288025643336
1106	24-25	0.16500201535877324
1106	26-27	0.20157938165163358
1106	28-29	0.21608668516177687
1106	30-31	0.20371068181400176
1106	32-33	0.2394124910549067
1106	34-35	0.2617057114365977
1106	36-37	0.26222871054656594
1106	38-39	0.208128103015099
1106	40-41	0.18140333272231857
1106	42-43	0.2760081455617751
1106	44-45	0.24930129003076473
1106	46-47	0.3218835525659145
1106	48-49	0.276911410658947
1106	50-51	0.3250677965467972
1106	52-53	0.23940573738029514
1106	54-55	0.21576413925720317
1106	56-57	0.21149850055414277
1106	58-59	0.18227125533034183
1106	60-61	0.1140596344524809
1106	62-63	0.21791013400483905
1106	64-65	0.26053481427549485
1106	66-67	0.1999037877457539
1106	68-69	0.13746470783364373
1106	70-71	0.210372630400947
1106	72-73	0.2374471167155221
1106	74-75	0.27610563518398834
1106	76-77	0.16645913454208028
1106	78-79	0.2787969632011169
1106	80-81	0.23538169310904777
1106	82-83	0.12894513849935407
1106	84-85	0.14854404172117341
1106	86-87	0.1807565854997577
1106	88-89	0.2965616282656285
1106	90-91	0.3073361740730789
1106	92-93	0.3225124676773454
1106	94-95	0.24302352722894227
1106	96-97	0.20158006122031935
1106	98-99	0.17097117044875887
1106	100	0.24237795165547737
1107	1	0.0027204309781900804
1107	2	0.040724078468723945
1107	3	-0.0075802784843581605
1107	4	-6.463714585223101E-4
1107	5	-0.10280916367883464
1107	6	-0.0507691190272368
1107	7	-0.05860834523534919
1107	8	-0.06567301757498711
1107	9	-0.027281788329581502
1107	10-11	-0.09225198121183809
1107	12-13	-0.06339616936983106
1107	14-15	-0.15171388801346808
1107	16-17	-0.15217688924753503
1107	18-19	-0.08838073205055252
1107	20-21	-0.09072641087340827
1107	22-23	-0.07908801848058289
1107	24-25	-0.12050597922198847
1107	26-27	-0.07902443449986407
1107	28-29	-0.1370459217971316
1107	30-31	-0.09854585926474613
1107	32-33	-0.10799043189307156
1107	34-35	-0.07400390618941799
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2216	10-11	-0.05370469407642986
2216	12-13	-0.04745520470099507
2216	14-15	-0.17338617461226846
2216	16-17	-0.13060874215661755
2216	18-19	-0.14588412847957244
2216	20-21	-0.14715242309797105
2216	22-23	-0.16410871473208033
2216	24-25	-0.17425334156965278
2216	26-27	-0.29966560760512095
2216	28-29	-0.16833936095805058
2216	30-31	-0.18594312569388904
2216	32-33	-0.1571789808544537
2216	34-35	-0.17305453022512296
2216	36-37	-0.19402756942071875
2216	38-39	-0.1939668374983512
2216	40-41	-0.21167278093737707
2216	42-43	-0.23164057629037416
2216	44-45	-0.16319782032621077
2216	46-47	-0.07180405152232083
2216	48-49	-0.05982889350955389
2216	50-51	-0.00250925748839137
2216	52-53	-0.0362144852366697
2216	54-55	-0.048260211595376745
2216	56-57	-0.058051546291267186
2216	58-59	-0.13029192757333163
2216	60-61	-0.0583835132523447
2216	62-63	-0.018423434498402003
2216	64-65	0.012336108719772199
2216	66-67	0.04256838447923883
2216	68-69	0.01865029995995826
2216	70-71	-0.008406538484500459
2216	72-73	0.12695505926593142
2216	74-75	0.11155452441676772
2216	76-77	0.039783366447103674
2216	78-79	0.04962984791104219
2216	80-81	0.06320481784192822
2216	82-83	0.0757748887459897
2216	84-85	0.038155237609430515
2216	86-87	0.018699376799908407
2216	88-89	0.040652208707058435
2216	90-91	0.05801108008115108
2216	92-93	0.08799563234653007
2216	94-95	0.019744972790270765
2216	96-97	-0.0422424583728116
2216	98-99	-0.06426504391670207
2216	100	-0.08806218348996708
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27854.0
3	5031.0
4	3311.0
5	2857.0
6	2435.0
7	2701.0
8	3260.0
9	3608.0
10	3917.0
11	4408.0
12	4860.0
13	5418.0
14	5924.0
15	6214.0
16	6193.0
17	6191.0
18	6390.0
19	6204.0
20	6282.0
21	6518.0
22	7420.0
23	10226.0
24	15563.0
25	21355.0
26	22467.0
27	20184.0
28	21293.0
29	27253.0
30	33743.0
31	41875.0
32	55466.0
33	75992.0
34	107359.0
35	167707.0
36	324721.0
37	674569.0
38	1018342.0
39	396155.0
40	644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.363410873670205	19.268918087488547	13.340034859358385	24.027636179482858
2	15.511500261866146	19.636161213285703	40.60020860630848	24.25212991853968
3	18.559816544073666	24.904186306759527	29.4559301318004	27.08006701736641
4	12.34606688674965	16.319520246938424	38.45083220855615	32.88358065775577
5	13.201490657760015	38.02041079783983	35.33734073641885	13.44075780798131
6	31.70635737739244	37.41219159715218	17.72476404926538	13.156686976190002
7	28.189625942860324	32.09688214024549	22.054866184050383	17.6586257328438
8	25.403075428111954	36.63424307288492	19.919332721997126	18.043348777006
9	25.349672967955385	16.986275630584906	20.55526963338944	37.10878176807027
10-11	24.418888433190578	26.58219359108213	28.472201023375742	20.52671695235155
12-13	24.874113002381566	23.973521581012264	28.461453542091125	22.690911874515045
14-15	22.20684175097808	25.833914318684485	25.48590831102181	26.473335619315623
16-17	22.11730251939618	29.181841949720262	26.081315528792615	22.619540002090943
18-19	22.04264000048082	28.30019830719236	27.792868469725807	21.86429322260101
20-21	23.128366690750987	27.471389850926066	27.359198460290607	22.04104499803234
22-23	22.79947782396708	27.245375920561425	27.363235618807703	22.59191063666379
24-25	22.658265969902125	27.431845437977504	27.282909328341802	22.62697926377857
26-27	22.42106237114584	27.448159802484913	27.362751292792233	22.76802653357701
28-29	22.368500967013976	27.44227176477361	27.482413757980158	22.706813510232255
30-31	22.554636176642013	27.80160711616677	27.14216912442141	22.501587582769805
32-33	22.363901461899875	27.520299946980348	27.514906241260935	22.600892349858846
34-35	22.530366732033368	27.32897544770972	27.569359796963084	22.571298023293824
36-37	22.881457120693703	27.555310600483462	27.055307042133236	22.507925236689598
38-39	22.59343122386786	27.69734456254578	27.227972296946046	22.48125191664032
40-41	22.388545240987472	27.503146759750763	27.486066721902663	22.622241277359098
42-43	22.436641538151083	27.413672257271127	27.47160153010456	22.67808467447323
44-45	22.382715498795392	27.586382494724994	27.480170685350835	22.55073132112878
46-47	22.54387612963954	27.565735121272965	27.384768227002038	22.505620522085458
48-49	22.495299691421916	27.581912039194616	27.26191626468861	22.660872004694852
50-51	22.4542709324199	27.52644351087274	27.30596033611744	22.713325220589923
52-53	22.440592417672455	27.508951405031763	27.417907301382865	22.63254887591291
54-55	22.59153728620953	27.783423297938924	27.272827905885226	22.35221150996632
56-57	22.413715670814284	27.565252098953323	27.287595032471867	22.733437197760527
58-59	22.372120848117223	27.2463959256165	27.601462266709493	22.780020959556786
60-61	22.66522423319854	27.48843098672222	27.2453089416379	22.601035838441337
62-63	22.305096667700884	27.610062714058493	27.340059225551432	22.74478139268919
64-65	22.51842679962743	27.411155231060448	27.28507338301967	22.785344586292453
66-67	22.346885703302075	27.37897851112586	27.29989495059136	22.974240834980712
68-69	22.58231070908976	27.53226010500348	27.24993674489215	22.635492441014613
70-71	22.61302734219644	27.32537076181565	27.278103898227435	22.783497997760477
72-73	22.44903251883513	27.442478591214716	27.31013495445459	22.798353935495562
74-75	22.468636873409547	27.463079971940335	27.30780616566208	22.76047698898804
76-77	22.525371720052075	27.413966401289397	27.221464563721543	22.83919731493699
78-79	22.56953877810625	27.50224002363213	27.27945532240052	22.648765875861102
80-81	22.5142873769943	27.366057471477035	27.358103127828198	22.761552023700464
82-83	22.410179685697493	27.39732872755668	27.418851097617154	22.773640489128674
84-85	22.432554967233223	27.35480612325419	27.26302341170761	22.949615497804977
86-87	22.548047226975964	27.41093231561273	27.261683635177903	22.779336822233404
88-89	22.37289812759632	27.430866661236458	27.264126389793926	22.9321088213733
90-91	22.395622187417068	27.27264338087186	27.40404840108777	22.927686030623303
92-93	22.545803927782863	27.366532291295766	27.240318720687924	22.84734506023345
94-95	22.327739661198134	27.193598111439695	27.46961234563581	23.009049881726366
96-97	22.40857244982837	27.163255238992416	27.42410525882103	23.004067052358188
98-99	22.350949264952554	27.29770825283806	27.3318125327184	23.019529949490988
100	22.48861047835991	27.17667120550769	27.256588742825873	23.078129573306523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	247.0
1	247.5
2	241.5
3	215.5
4	188.5
5	159.0
6	142.0
7	137.5
8	143.5
9	163.0
10	166.5
11	163.0
12	175.0
13	192.0
14	228.0
15	272.5
16	320.5
17	447.0
18	631.0
19	859.5
20	1227.5
21	1833.5
22	2866.5
23	4717.0
24	6993.5
25	9815.5
26	13983.0
27	19370.0
28	26504.5
29	34497.5
30	42086.5
31	50296.5
32	58501.5
33	66597.5
34	75511.5
35	83920.0
36	90930.0
37	97410.0
38	102973.0
39	106929.0
40	110350.5
41	113008.0
42	113632.5
43	114779.0
44	115294.5
45	113823.0
46	113602.0
47	111694.5
48	107404.0
49	104470.5
50	102825.0
51	100008.5
52	94490.5
53	89616.5
54	86586.0
55	83257.0
56	77502.5
57	70405.5
58	65019.5
59	60137.5
60	54712.0
61	48396.5
62	41893.0
63	35814.5
64	29743.5
65	24269.5
66	19725.0
67	14708.5
68	10635.0
69	8216.5
70	5968.0
71	4387.0
72	3478.0
73	2771.0
74	2136.5
75	1555.0
76	1046.5
77	775.0
78	554.5
79	345.0
80	223.5
81	125.5
82	79.0
83	60.0
84	47.5
85	35.0
86	27.0
87	21.0
88	18.0
89	11.5
90	6.5
91	7.5
92	6.0
93	4.0
94	4.0
95	3.0
96	1.5
97	1.5
98	2.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32856722677116046
2	0.12043353542637203
3	0.06742759914102552
4	0.040608366462043514
5	0.04509932287762776
6	0.010721367780866627
7	0.008033119222242252
8	0.01673039397073288
9	0.01894424572489413
10-11	0.022312463036582318
12-13	0.01717316432156513
14-15	0.019418642529357256
16-17	0.020762766808669446
18-19	0.02065207422096138
20-21	0.025111404182914757
22-23	0.016572261702578504
24-25	0.026028571338210134
26-27	0.020003731921528444
28-29	0.019987918694713006
30-31	0.020794393262300318
32-33	0.02587043907005576
34-35	0.01627181039308519
36-37	0.010515795832265942
38-39	0.014342596721601816
40-41	0.01026278420321894
42-43	0.009361430274739003
44-45	0.009582815450155128
46-47	0.008918659923906752
48-49	0.004728154817815813
50-51	0.006878753664715314
52-53	0.008080558902688566
54-55	0.0035105363530271256
56-57	0.003036139548564001
58-59	0.003368217311688189
60-61	0.0039058670234130635
62-63	0.003131018909456626
64-65	0.0023719840223156256
66-67	0.002039906259191438
68-69	0.003478909899396251
70-71	0.0023877972491310635
72-73	0.002071532712822313
74-75	0.0024036104759465006
76-77	0.0038584273429667516
78-79	0.003573789260288876
80-81	0.0036528553943660637
82-83	0.0026408088781780634
84-85	0.0029886998681176885
86-87	0.0023087311150538758
88-89	0.6008393660793634
90-91	0.6115291074065993
92-93	0.6078920652390485
94-95	0.5937866669196783
96-97	0.5921895310113191
98-99	0.5885524888437685
100	0.5904658892884364
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3161910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.67632272754071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2028642267753	38.21907919361654
2	15.132257802117069	16.244879067655628
3	5.748701900916908	9.257075354941279
4	2.7236045247700105	5.847723018149808
5	1.513549546201279	4.0620886953011315
6	0.8711954526371822	2.805754096471157
7	0.5954294520348027	2.2372324400231953
8	0.4119861617445736	1.769112174166601
9	0.2841752481265862	1.3728134096659435
>10	1.4160601714725198	13.293772454129963
>50	0.07535759086358348	2.7321546208188585
>100	0.024700049273772836	2.1237700945935982
>500	1.1787306652691116E-4	0.034545380466340835
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	1.5813226815437504E-5	0.0	0.0	0.0	0.0
16-17	3.162645363087501E-5	0.0	0.0	0.0	0.0
18-19	3.162645363087501E-5	0.0	0.0	0.0	0.0
20-21	3.162645363087501E-5	0.0	0.0	0.0	0.0
22-23	6.325290726175002E-5	0.0	0.0	0.0	0.0
24-25	6.325290726175002E-5	0.0	0.0	3.162645363087501E-5	0.0
26-27	9.487936089262503E-5	0.0	0.0	1.4231904133893755E-4	0.0
28-29	9.487936089262503E-5	0.0	0.0	7.906613407718752E-4	0.0
30-31	1.1069258770806253E-4	0.0	0.0	0.004285384466983564	0.0
32-33	1.4231904133893755E-4	0.0	0.0	0.012160371421071441	0.0
34-35	1.5813226815437504E-4	0.0	0.0	0.023609147635448195	0.0
36-37	1.5813226815437504E-4	0.0	0.0	0.04451423348545658	0.0
38-39	1.5813226815437504E-4	0.0	0.0	0.08004655413974465	0.0
40-41	1.5813226815437504E-4	0.0	0.0	0.12139814226211373	0.0
42-43	1.5813226815437504E-4	0.0	0.0	0.16287623619900632	0.0
44-45	1.5813226815437504E-4	0.0	0.0	0.21083775313022823	0.0
46-47	1.5813226815437504E-4	0.0	0.0	0.2708015092143673	0.0
48-49	1.5813226815437504E-4	0.0	0.0	0.3380867893140539	0.0
50-51	1.5813226815437504E-4	0.0	0.0	0.41161829400583827	0.0
52-53	1.5813226815437504E-4	0.0	0.0	0.483030826304354	0.0
54-55	1.5813226815437504E-4	0.0	0.0	0.5596459102251488	0.0
56-57	1.5813226815437504E-4	0.0	0.0	0.6525802442194749	0.0
58-59	1.5813226815437504E-4	0.0	0.0	0.7606003965957285	0.0
60-61	1.5813226815437504E-4	0.0	0.0	0.8760053258947915	0.0
62-63	1.5813226815437504E-4	0.0	0.0	0.9917423329569786	0.0
64-65	1.5813226815437504E-4	0.0	0.0	1.1178370035832772	0.0
66-67	1.5813226815437504E-4	0.0	0.0	1.2570250260127582	0.0
68-69	1.5813226815437504E-4	0.0	0.0	1.420280779655335	0.0
70-71	1.5813226815437504E-4	0.0	0.0	1.6007887637535538	0.0
72-73	1.5813226815437504E-4	0.0	0.0	1.7867839375567298	0.0
74-75	1.7394549496981253E-4	0.0	0.0	1.9765110328883493	0.0
76-77	2.0557194860068757E-4	0.0	0.0	2.1816718375918356	0.0
78-79	2.2138517541612506E-4	0.0	0.0	2.409034412744196	0.0
80-81	2.2138517541612506E-4	0.0	0.0	2.6628999560392295	0.0
82-83	2.2138517541612506E-4	0.0	0.0	2.9252413889073376	0.0
84-85	2.2138517541612506E-4	0.0	0.0	3.190160377746362	0.0
86-87	2.2138517541612506E-4	0.0	0.0	3.4737389742276026	0.0
88	2.2138517541612506E-4	0.0	0.0	3.7046911518670678	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2555	0.0	40.010357	1
GTATCAA	4325	0.0	36.815796	1
TCAACGC	4530	0.0	34.120678	4
ATCAACG	4675	0.0	32.967632	3
CAACGCA	4890	0.0	31.320501	5
AACGCAG	5015	0.0	30.713581	6
GTACATG	10310	0.0	28.100887	1
TATCAAC	5930	0.0	26.718882	2
TACATGG	10180	0.0	26.694584	2
ACGCAGA	5795	0.0	26.093773	7
CGCAGAG	6190	0.0	24.35318	8
ACATGGG	10805	0.0	24.092426	3
TATACCG	230	4.123649E-9	22.469055	5
CATGGGG	7460	0.0	21.223207	4
GCAGAGT	7325	0.0	19.746237	9
GAGTACA	7340	0.0	18.997622	1
AGTACAT	7335	0.0	17.81918	2
GTGTATA	1660	0.0	17.594915	1
AGAGTAC	6270	0.0	17.039434	10-11
GTATAGA	1165	0.0	16.98349	1
>>END_MODULE