FastQCFastQC Report
Wed 25 May 2016
SRR1033186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161910
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48330.15285065039801893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40450.1279290049368894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43700.049.5288731
GTATCAA79250.041.619881
ATCAACG94350.034.4800763
TCAACGC94550.034.4568634
CAACGCA98000.033.2918135
AACGCAG102750.031.9241736
TATCAAC102900.031.8440512
ACGCAGA117900.027.58287
CGCAGAG119200.027.2421268
ACCGTCG2253.6379788E-1227.1518888
GTACATG108250.025.9929371
TACATGG105100.025.7650342
GCAGAGT134750.023.8539149
ACATGGG110450.023.6227973
ATACCGT5000.021.6174226
CATGGGG73550.020.9650174
TAGACCG1354.072502E-420.8939975
CCGTCGT2751.4424586E-920.5059959
GAGTACT82750.020.0465212-13
AGAGTAC125100.019.75880810-11
Produced by FastQC (version 0.11.5)