##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033186_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3161910
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73321536666129	34.0	31.0	34.0	31.0	34.0
2	32.830191877694176	34.0	31.0	34.0	31.0	34.0
3	32.92556556005705	34.0	31.0	34.0	31.0	34.0
4	36.348707901236914	37.0	37.0	37.0	35.0	37.0
5	36.28841712762223	37.0	37.0	37.0	35.0	37.0
6	36.371122201454185	37.0	37.0	37.0	35.0	37.0
7	36.34332950653244	37.0	37.0	37.0	35.0	37.0
8	36.34258818245934	37.0	37.0	37.0	35.0	37.0
9	38.16263334503512	39.0	39.0	39.0	37.0	39.0
10-11	38.137468334013306	39.0	39.0	39.0	37.0	39.0
12-13	38.10096017913223	39.0	39.0	39.0	37.0	39.0
14-15	39.62579548437495	41.0	40.0	41.0	37.0	41.0
16-17	39.637799304850546	41.0	40.0	41.0	37.0	41.0
18-19	39.64863753237758	41.0	40.0	41.0	37.0	41.0
20-21	39.64017429338596	41.0	40.0	41.0	37.0	41.0
22-23	39.56994886002448	41.0	40.0	41.0	37.0	41.0
24-25	39.51903596244043	41.0	39.5	41.0	37.0	41.0
26-27	39.419872482138956	41.0	39.0	41.0	36.0	41.0
28-29	39.33120171035861	41.0	39.0	41.0	36.0	41.0
30-31	39.24178060096587	41.0	39.0	41.0	36.0	41.0
32-33	39.28823733123333	41.0	39.0	41.0	35.5	41.0
34-35	39.23987447460554	41.0	39.0	41.0	35.0	41.0
36-37	39.15630441726678	41.0	39.0	41.0	35.0	41.0
38-39	39.0707055229276	41.0	39.0	41.0	35.0	41.0
40-41	38.96107716538421	41.0	39.0	41.0	35.0	41.0
42-43	38.84515403664241	41.0	39.0	41.0	35.0	41.0
44-45	38.70264650163983	41.0	39.0	41.0	35.0	41.0
46-47	38.49789067367509	41.0	38.0	41.0	35.0	41.0
48-49	38.3173393613354	40.5	38.0	41.0	34.5	41.0
50-51	38.177012786575204	40.0	38.0	41.0	34.0	41.0
52-53	38.02989063572335	40.0	38.0	41.0	34.0	41.0
54-55	37.881977665398445	40.0	37.0	41.0	34.0	41.0
56-57	37.70772175678624	40.0	37.0	41.0	33.5	41.0
58-59	37.509197288980396	40.0	36.5	41.0	33.0	41.0
60-61	37.300224547820775	39.5	36.0	41.0	33.0	41.0
62-63	37.05102327390723	39.0	35.0	41.0	33.0	41.0
64-65	36.79100670164552	39.0	35.0	41.0	33.0	41.0
66-67	36.499517222185325	38.5	35.0	41.0	32.0	41.0
68-69	36.17267822297282	37.0	35.0	40.0	32.0	41.0
70-71	35.83533291586414	37.0	35.0	40.0	32.0	41.0
72-73	35.48483938505524	36.5	35.0	39.0	31.0	41.0
74-75	35.147274274093824	36.0	35.0	39.0	31.0	41.0
76-77	34.81731817161146	35.5	35.0	38.5	31.0	40.0
78-79	34.49004462492607	35.0	35.0	37.0	31.0	39.0
80-81	34.168973816459044	35.0	34.5	37.0	31.0	39.0
82-83	33.86744704941001	35.0	34.0	36.0	30.5	38.5
84-85	33.613193291396655	35.0	34.0	36.0	30.0	37.0
86-87	33.40209983838882	35.0	34.0	36.0	30.0	37.0
88-89	33.200682498869355	35.0	34.0	35.0	30.0	36.5
90-91	33.01817888554703	35.0	34.0	35.0	30.0	36.0
92-93	32.87005338545373	35.0	34.0	35.0	30.0	36.0
94-95	32.73222387733996	35.0	34.0	35.0	29.0	36.0
96-97	32.61392860644358	35.0	34.0	35.0	29.0	36.0
98-99	32.515957443443995	35.0	34.0	35.0	29.0	35.0
100	32.420655553130864	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14197078710463984
1101	2	0.15265180808520284
1101	3	0.16083025723570898
1101	4	0.118110482264143
1101	5	0.07272986647621593
1101	6	0.0972489296835235
1101	7	0.09976665824869713
1101	8	0.14688067173945285
1101	9	0.17660928628813366
1101	10-11	0.14388470657757324
1101	12-13	0.16800231281462885
1101	14-15	0.19237141183401718
1101	16-17	0.2138985587722928
1101	18-19	0.2278356586688446
1101	20-21	0.20959531104932694
1101	22-23	0.24627218794289973
1101	24-25	0.2318991277984992
1101	26-27	0.2039564082319032
1101	28-29	0.24224376751134002
1101	30-31	0.20544187115905288
1101	32-33	0.19443948575683834
1101	34-35	0.2196354044136939
1101	36-37	0.25249096146947636
1101	38-39	0.2178749125921513
1101	40-41	0.27103423891045253
1101	42-43	0.2184002570203063
1101	44-45	0.1991168841709552
1101	46-47	0.1909716904422254
1101	48-49	0.1576920769490897
1101	50-51	0.10458449571730455
1101	52-53	0.20176693083663366
1101	54-55	0.2079926382589221
1101	56-57	0.19830314261484006
1101	58-59	0.16929734507409222
1101	60-61	0.15763281429382658
1101	62-63	0.14754697423015273
1101	64-65	0.26944833869539764
1101	66-67	0.22067443137419218
1101	68-69	0.1987893773410363
1101	70-71	0.22162271156125968
1101	72-73	0.2605774696153915
1101	74-75	0.22451369510130803
1101	76-77	0.20466542376516372
1101	78-79	0.2646589994839772
1101	80-81	0.2172646326579155
1101	82-83	0.20346539570725497
1101	84-85	0.20427944329919967
1101	86-87	0.14200155145719862
1101	88-89	0.15336229602943519
1101	90-91	0.18031488085836145
1101	92-93	0.22185676076274774
1101	94-95	0.2273880863800528
1101	96-97	0.22370333935337783
1101	98-99	0.1972922147773346
1101	100	0.20786329762819378
1102	1	0.08974584177171607
1102	2	0.12470777557428647
1102	3	0.10761062007760103
1102	4	0.0650066718632516
1102	5	0.07522053572479592
1102	6	0.08935758180046349
1102	7	0.07011926326338624
1102	8	0.08572603498161868
1102	9	0.16937145345561078
1102	10-11	0.1449575617654375
1102	12-13	0.1409350967351628
1102	14-15	0.15981692283015292
1102	16-17	0.15287889564377366
1102	18-19	0.1720519165222143
1102	20-21	0.16937206639988744
1102	22-23	0.2221250668445336
1102	24-25	0.23285631381659044
1102	26-27	0.24565932000626844
1102	28-29	0.2558343434051338
1102	30-31	0.2669292537643031
1102	32-33	0.24900350123569837
1102	34-35	0.3093590654090974
1102	36-37	0.29137999218704635
1102	38-39	0.26808723012955227
1102	40-41	0.32490833664365937
1102	42-43	0.2683258442383263
1102	44-45	0.2933240649886528
1102	46-47	0.30045642053594435
1102	48-49	0.3062824416757337
1102	50-51	0.30890171353023277
1102	52-53	0.3500033542072245
1102	54-55	0.28852603702255664
1102	56-57	0.24968743783029623
1102	58-59	0.3042782234159702
1102	60-61	0.2697294771594798
1102	62-63	0.2869302148705657
1102	64-65	0.2549613267357671
1102	66-67	0.28360492082182986
1102	68-69	0.26976959003363277
1102	70-71	0.29568612221958546
1102	72-73	0.3011585953064966
1102	74-75	0.3371690841815109
1102	76-77	0.2719245245965354
1102	78-79	0.2868285559888406
1102	80-81	0.29829821390778477
1102	82-83	0.23598316684115161
1102	84-85	0.2679373562995906
1102	86-87	0.28501844293808887
1102	88-89	0.3000230815334106
1102	90-91	0.27493076466221567
1102	92-93	0.2860433007795464
1102	94-95	0.3301512400448132
1102	96-97	0.349348896238979
1102	98-99	0.34805397662995574
1102	100	0.35940771742596667
1103	1	0.15443228672907594
1103	2	0.17772969568039798
1103	3	0.14666147967393783
1103	4	0.10344001907992606
1103	5	0.031049123738128515
1103	6	0.079832962283092
1103	7	0.10589547255469256
1103	8	0.09567017084580698
1103	9	0.13393177207490936
1103	10-11	0.10603915191435931
1103	12-13	0.0790421601619542
1103	14-15	0.11931077479949437
1103	16-17	0.1675771982462564
1103	18-19	0.16788108158414872
1103	20-21	0.11767155241444982
1103	22-23	0.15508450987803002
1103	24-25	0.134685241222968
1103	26-27	0.15562418036000025
1103	28-29	0.18449067768476368
1103	30-31	0.21207510380465067
1103	32-33	0.20400873940949538
1103	34-35	0.2723283785875523
1103	36-37	0.23671323222750118
1103	38-39	0.17650554492846737
1103	40-41	0.2319815451113172
1103	42-43	0.18499469745676578
1103	44-45	0.18584650026647154
1103	46-47	0.11197321993886789
1103	48-49	0.10937108931004502
1103	50-51	0.08928256862578365
1103	52-53	0.08374433116258473
1103	54-55	0.07356620774852018
1103	56-57	0.10078499634166604
1103	58-59	0.12357135596453617
1103	60-61	0.13836189910787056
1103	62-63	0.05546337742163132
1103	64-65	0.03660485264751401
1103	66-67	0.01706307861526568
1103	68-69	0.09344849304613945
1103	70-71	0.13866463701108245
1103	72-73	0.1627913623782078
1103	74-75	0.11418312707150591
1103	76-77	0.07278366853989837
1103	78-79	0.0914329866565069
1103	80-81	0.08424158131211357
1103	82-83	0.026736194159781235
1103	84-85	0.058814640077521574
1103	86-87	0.044656185093664647
1103	88-89	0.029224751878011546
1103	90-91	0.049621960720109826
1103	92-93	0.0022100814987666695
1103	94-95	-0.012213306088860065
1103	96-97	0.011045455330311427
1103	98-99	0.037516274897939184
1103	100	0.04739989892001972
1104	1	0.10335171952115729
1104	2	0.10981012538559298
1104	3	0.08656577545825428
1104	4	0.040072166209583315
1104	5	0.04604383893089903
1104	6	0.06832289115228463
1104	7	0.06850818293457905
1104	8	0.06479766528401143
1104	9	0.0697934298235765
1104	10-11	0.09329745895106356
1104	12-13	0.09110364526294035
1104	14-15	0.1379231372133134
1104	16-17	0.07435843374062046
1104	18-19	0.11049706454813446
1104	20-21	0.0906429058853746
1104	22-23	0.10265230834722416
1104	24-25	0.04030125969961773
1104	26-27	5.604225451563138E-4
1104	28-29	0.05817144746001901
1104	30-31	0.08798175329493318
1104	32-33	0.09894321079959667
1104	34-35	0.10639651123384652
1104	36-37	0.0637663070195984
1104	38-39	0.1008644856678842
1104	40-41	0.04108219313521744
1104	42-43	0.06017507906019404
1104	44-45	0.15941196515678513
1104	46-47	0.21823338205273757
1104	48-49	0.056920478284148146
1104	50-51	0.07464619752094137
1104	52-53	0.05414273392479174
1104	54-55	0.011868054958704022
1104	56-57	0.09415208670572639
1104	58-59	0.06726846737202408
1104	60-61	0.0692489774421361
1104	62-63	0.06840167218082627
1104	64-65	0.05391967223784633
1104	66-67	0.05582275859872965
1104	68-69	0.06265487495282684
1104	70-71	0.04970683602503101
1104	72-73	0.05845918967363417
1104	74-75	0.06188496892092843
1104	76-77	0.080407787968511
1104	78-79	0.0402319231344066
1104	80-81	0.09805712117092469
1104	82-83	0.13659636811765097
1104	84-85	0.07236765714943516
1104	86-87	0.0931302484132317
1104	88-89	0.08179474642337681
1104	90-91	0.09977922280387475
1104	92-93	0.1333006367649432
1104	94-95	0.021900335022841944
1104	96-97	0.11029097084020378
1104	98-99	0.12861729430497348
1104	100	0.08514565358400716
1105	1	0.07694221207242435
1105	2	0.031279809911275436
1105	3	0.06486869917161897
1105	4	0.05415270247414128
1105	5	0.04750068515912176
1105	6	0.06902491285382695
1105	7	-0.021657595605624635
1105	8	-0.021238048406885923
1105	9	0.040267671768070556
1105	10-11	-0.021577203890103647
1105	12-13	0.04804577922840991
1105	14-15	0.018900847349712535
1105	16-17	0.07721529919185599
1105	18-19	0.07025761327323465
1105	20-21	0.09071334066709369
1105	22-23	0.07863844413971322
1105	24-25	0.034521490373990105
1105	26-27	0.02128567097663847
1105	28-29	0.04364962379651871
1105	30-31	0.08619673867157474
1105	32-33	0.01504682566704929
1105	34-35	0.10228610206173272
1105	36-37	0.045703484032628694
1105	38-39	0.00615973440920925
1105	40-41	0.09832862024484967
1105	42-43	0.06854960712622216
1105	44-45	0.13927927142461272
1105	46-47	0.1731500740818177
1105	48-49	0.10232609283877991
1105	50-51	0.09046638915378935
1105	52-53	0.09889398888744694
1105	54-55	0.10934963002929976
1105	56-57	0.06756116549787095
1105	58-59	0.06028912020848054
1105	60-61	0.052282685370037996
1105	62-63	0.0698909677222872
1105	64-65	0.08183934048537367
1105	66-67	0.023966697890458022
1105	68-69	0.014995440427824747
1105	70-71	0.08605471908766305
1105	72-73	0.07269608743182943
1105	74-75	0.07183434706182368
1105	76-77	0.024123535334119595
1105	78-79	0.030277101882902002
1105	80-81	0.09968414601969755
1105	82-83	0.05437671692520496
1105	84-85	0.10229083206282041
1105	86-87	0.13023014140261324
1105	88-89	0.018801836702998287
1105	90-91	0.013173401429646958
1105	92-93	0.09562572798822089
1105	94-95	0.09815398807115372
1105	96-97	0.09935643620150358
1105	98-99	-0.034391772614668525
1105	100	0.006946949155988591
1106	1	0.06313707278407321
1106	2	0.07575105937508653
1106	3	0.005323902446065176
1106	4	0.03133988886321504
1106	5	-0.007531944854257233
1106	6	-0.016821843899677447
1106	7	-0.01627532489878547
1106	8	-0.03129858687002951
1106	9	0.022065420473097674
1106	10-11	0.020832993974593705
1106	12-13	0.06199646294199823
1106	14-15	0.11047118595264038
1106	16-17	0.07681216229189403
1106	18-19	0.0943462512758515
1106	20-21	0.08820280512151157
1106	22-23	0.13089414887985384
1106	24-25	0.06236426141512652
1106	26-27	0.01777218178121842
1106	28-29	0.04747188543439762
1106	30-31	0.10105875097525541
1106	32-33	0.10345160086048821
1106	34-35	0.12132743311505578
1106	36-37	0.09062049809798367
1106	38-39	0.10995669559305554
1106	40-41	0.12045210784116733
1106	42-43	0.1503817876111171
1106	44-45	0.1959488809023071
1106	46-47	0.20055739223814584
1106	48-49	0.18134535726372292
1106	50-51	0.1369473766198226
1106	52-53	0.15784663026820311
1106	54-55	0.14705668284265982
1106	56-57	0.13460921720700725
1106	58-59	0.1575555798687347
1106	60-61	0.13899658400519144
1106	62-63	0.16838441031676155
1106	64-65	0.14837098231112122
1106	66-67	0.15688472664656672
1106	68-69	0.13160636888767385
1106	70-71	0.14245312818798794
1106	72-73	0.2425636475727444
1106	74-75	0.18388460142492846
1106	76-77	0.10223073620858969
1106	78-79	0.11398977870211269
1106	80-81	0.16559775298694035
1106	82-83	0.23943748923938557
1106	84-85	0.2058674387641375
1106	86-87	0.2058705034663646
1106	88-89	0.2151633151581933
1106	90-91	0.1758505769902996
1106	92-93	0.20525319661989272
1106	94-95	0.13908054593101582
1106	96-97	0.17288162685690622
1106	98-99	0.22784262272897848
1106	100	0.19361539160569663
1107	1	-0.07725587367060882
1107	2	-0.06388309892383859
1107	3	-0.007719801944155336
1107	4	-0.015595202115484597
1107	5	-0.042290316932010796
1107	6	-0.10822382214596615
1107	7	-0.15128104170825196
1107	8	-0.12021752583702039
1107	9	-0.08240643756245447
1107	10-11	-0.06476682765754305
1107	12-13	-0.08834424355504211
1107	14-15	-0.03042521062426573
1107	16-17	-0.008167805427952146
1107	18-19	-0.012462677689917712
1107	20-21	-0.07980522995863737
1107	22-23	-0.10302281627446774
1107	24-25	-0.0434479987325318
1107	26-27	-0.012222907134137984
1107	28-29	-0.09579453579172537
1107	30-31	-0.04248038039369817
1107	32-33	0.0012165670925199379
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2216	7	-0.11592870172821534
2216	8	-0.1292369874396968
2216	9	-0.13417074899961534
2216	10-11	-0.16117561375506284
2216	12-13	-0.13515257334193365
2216	14-15	-0.2556285241423595
2216	16-17	-0.254748266094488
2216	18-19	-0.19331225573709077
2216	20-21	-0.18168736821927212
2216	22-23	-0.22279984968869115
2216	24-25	-0.2591326893448169
2216	26-27	-0.27403120400850867
2216	28-29	-0.33075490911701166
2216	30-31	-0.2884570751810145
2216	32-33	-0.2550122347400503
2216	34-35	-0.2679636737760589
2216	36-37	-0.2670911283592545
2216	38-39	-0.2023646868994291
2216	40-41	-0.23619092504040395
2216	42-43	-0.22687424672344747
2216	44-45	-0.17989617047435047
2216	46-47	-0.22263845733535703
2216	48-49	-0.2292177803822142
2216	50-51	-0.16773752915831608
2216	52-53	-0.14159109709261486
2216	54-55	-0.16618602308825814
2216	56-57	-0.17384983713257185
2216	58-59	-0.17910079674572188
2216	60-61	-0.14452003708134242
2216	62-63	-0.194652737891019
2216	64-65	-0.19243989034930564
2216	66-67	-0.1745878688810194
2216	68-69	-0.15034495495589795
2216	70-71	-0.15762848173192623
2216	72-73	-0.11764772065990314
2216	74-75	-0.1253393999211312
2216	76-77	-0.09353299358374301
2216	78-79	-0.12250374305425993
2216	80-81	-0.1405803661379892
2216	82-83	-0.147984308857815
2216	84-85	-0.16017254206701637
2216	86-87	-0.18645006146699217
2216	88-89	-0.1387230835413007
2216	90-91	-0.12658697189444013
2216	92-93	-0.1294247599056746
2216	94-95	-0.09683345779836117
2216	96-97	-0.08450730098364545
2216	98-99	-0.04995334736149459
2216	100	-0.09509773018178436
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6.0
3	6.0
4	13.0
5	36.0
6	61.0
7	159.0
8	328.0
9	644.0
10	1168.0
11	1794.0
12	2659.0
13	3993.0
14	4822.0
15	6742.0
16	8433.0
17	12354.0
18	14241.0
19	7992.0
20	5744.0
21	5507.0
22	5768.0
23	6181.0
24	7071.0
25	8754.0
26	11260.0
27	14301.0
28	18599.0
29	24018.0
30	30809.0
31	38970.0
32	50679.0
33	66601.0
34	94389.0
35	149297.0
36	292688.0
37	661479.0
38	1111868.0
39	490994.0
40	1482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16454189597877	19.368260313267825	13.482633901071386	23.984563889682015
2	15.798289242392327	19.929826425720947	40.10434950918348	24.167534822703246
3	18.591990284353443	25.058588005351197	29.73073237378673	26.618689336508627
4	12.473888400962837	16.578592236525466	38.11773204099169	32.82978732152
5	12.972646669484597	37.8507121180291	35.327957751291486	13.848683461194819
6	31.45078678834437	37.613147770094294	17.432275795762365	13.503789645798973
7	27.90258488288831	32.33013933976181	21.97257989833536	17.794695879014526
8	25.711464619454233	35.98806158209655	19.78229497329606	18.518178825153157
9	25.604310560487352	16.24347822786804	20.698761412804735	37.45344979883988
10-11	24.59938619145842	26.189854954207885	28.31781733629611	20.892941518037585
12-13	25.33214101603145	23.624012068654707	28.661932186558126	22.38191472875572
14-15	22.385272558617316	25.35300690374511	26.11956961416006	26.14215092347751
16-17	21.72987213424797	28.8934062006825	26.65494906559643	22.721772599473102
18-19	21.579900740296797	27.51563731703094	29.156656227293176	21.747805715379087
20-21	22.724192023175867	26.682685465430705	28.886559073471414	21.706563437922018
22-23	22.328446980400876	26.489360911464193	28.94109762748776	22.241094480647167
24-25	22.17494413576113	26.706102789566994	28.850979622578972	22.267973452092907
26-27	21.894762376680713	26.814471908733566	28.94536718615462	22.345398528431097
28-29	21.96544928174321	26.778483008514957	29.008942587217344	22.247125122524483
30-31	22.111539919346928	27.102288798233808	28.66866749053532	22.117503791883944
32-33	21.93685683052918	26.824165796571997	29.018955541576396	22.220021831322427
34-35	22.109884192896683	26.71144967361037	29.00193486380343	22.17673126968952
36-37	22.426808805720654	26.941721005391905	28.475964841906258	22.15550534698118
38-39	22.22322048136724	27.043409667995704	28.57655117739833	22.156818673238725
40-41	22.086371790045146	26.906595336094174	28.710734702787523	22.296298171073154
42-43	22.165619784305115	26.87741290145429	28.533038164809316	22.42392914943128
44-45	22.141650877435232	27.02274962130361	28.504715100262608	22.330884400998553
46-47	22.357502861496304	27.144698794399453	28.21550020790034	22.282298136203906
48-49	22.37903898583778	27.202186399025415	27.894173909564167	22.524600705572638
50-51	22.39674497839411	27.216888840531396	27.748021753281055	22.63834442779344
52-53	22.341911056555663	27.248011128130695	27.890684080833296	22.519393734480346
54-55	22.47119528630444	27.478761470450607	27.72335582762226	22.326687415622697
56-57	22.23559003777251	27.24527906843615	27.83603003710201	22.68310085668932
58-59	22.188894641445795	26.941005648856265	28.13970743345966	22.730392276238277
60-61	22.481411434300373	27.175787266476696	27.81042351251941	22.532377786703524
62-63	22.12233387051655	27.301289255529216	27.912084123808633	22.664292750145602
64-65	22.304529664441713	27.06316247075826	27.912823446082214	22.719484418717812
66-67	22.17831739877099	27.04199070808915	27.853340881561344	22.926351011578518
68-69	22.44204428668997	27.197892516640838	27.807309301077794	22.552753895591398
70-71	22.434499784623032	26.98809386008024	27.78713924358091	22.790267111715814
72-73	22.31311542473864	27.167409128365094	27.74127448712266	22.778200959773603
74-75	22.360211025617264	27.164393079963855	27.681599397322675	22.7937964970962
76-77	22.389110459497964	27.127500852731895	27.62011659774442	22.863272090025717
78-79	22.446940652122176	27.25718573361175	27.64706114900578	22.64881246526029
80-81	22.36422781146635	27.199711311585663	27.6816862338354	22.754374643112584
82-83	22.26165184300225	27.231918858721478	27.71322870717458	22.79320059110169
84-85	22.247261655684085	27.152869879090165	27.54820161222606	23.05166685299969
86-87	22.387142061284862	27.17863415632309	27.566991623566352	22.867232158825697
88-89	22.1362562501779	27.202988067339046	27.585225385921802	23.075530296561254
90-91	22.147687408994823	27.055863105441396	27.699003260056905	23.097446225506875
92-93	22.18205837043381	27.181032091688913	27.553417887738075	23.083491650139205
94-95	22.044112577524345	26.945074337979257	27.73535616130756	23.27545692318883
96-97	22.04631061605169	26.95100429803505	27.695000806474567	23.307684279438693
98-99	21.98171358451063	27.06606133634417	27.632143229883205	23.320081849261996
100	22.050880638601353	26.950767099632817	27.50305985938879	23.495292402377043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	22.0
2	77.0
3	150.5
4	121.0
5	109.0
6	233.5
7	537.0
8	952.5
9	1176.0
10	1038.0
11	887.0
12	970.5
13	1088.5
14	1259.0
15	1465.5
16	1635.0
17	1904.5
18	2228.5
19	2522.5
20	2850.0
21	3444.5
22	4562.5
23	6267.0
24	8534.5
25	11217.5
26	14958.0
27	20245.5
28	27187.5
29	34833.0
30	42008.0
31	49700.5
32	57962.0
33	66115.5
34	74437.5
35	82690.5
36	89527.5
37	96037.5
38	101663.0
39	105324.5
40	108989.5
41	111910.5
42	113271.5
43	114876.5
44	115291.0
45	113710.5
46	113717.5
47	111478.5
48	106945.0
49	104160.0
50	102191.0
51	99069.5
52	93566.0
53	89058.0
54	85835.0
55	82062.5
56	76653.5
57	70227.5
58	64432.5
59	59882.5
60	55023.0
61	48275.5
62	41749.5
63	35594.0
64	29321.5
65	23732.0
66	19057.0
67	14273.0
68	10328.5
69	7905.0
70	5737.0
71	4162.5
72	3154.5
73	2443.0
74	1896.0
75	1383.5
76	895.0
77	608.0
78	436.0
79	286.5
80	188.5
81	89.5
82	40.5
83	16.5
84	8.5
85	8.0
86	4.0
87	1.5
88	2.5
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08931310505359102
2	0.0011385523307115002
3	0.0
4	3.162645363087501E-5
5	0.03788849144978826
6	1.5813226815437504E-4
7	0.004490956415584251
8	5.376497117248752E-4
9	3.162645363087501E-5
10-11	3.795174435705001E-4
12-13	0.0
14-15	9.487936089262503E-5
16-17	0.0
18-19	7.906613407718752E-5
20-21	0.0
22-23	2.688248558624376E-4
24-25	1.1069258770806253E-4
26-27	0.0014706300938356878
28-29	0.009393056728369879
30-31	0.03833126180062051
32-33	0.02705643108121357
34-35	0.030487901300163506
36-37	0.027641520473384758
38-39	0.026929925266690073
40-41	0.03289151177611001
42-43	0.024605380924820754
44-45	0.023292883099139444
46-47	0.017062471733857068
48-49	0.011527842348453941
50-51	0.008602395387598003
52-53	0.006103905550758877
54-55	0.004570022549661439
56-57	0.002751501465886126
58-59	9.804200625571254E-4
60-61	6.325290726175002E-4
62-63	0.0014706300938356878
64-65	0.003779361208889564
66-67	6.325290726175002E-4
68-69	0.0010911126502651878
70-71	3.795174435705001E-4
72-73	5.850893921711877E-4
74-75	6.009026189866251E-4
76-77	4.269571240168126E-4
78-79	7.906613407718752E-5
80-81	7.432216603255626E-4
82-83	5.534629385403126E-4
84-85	2.688248558624376E-4
86-87	1.1069258770806253E-4
88-89	0.0
90-91	6.325290726175002E-5
92-93	3.162645363087501E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3161910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.61186262716747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.51598227191347	35.87849327410607
2	15.948476736458506	16.46261180869345
3	6.071293111628718	9.400522381399485
4	2.8091969511228547	5.799515485360418
5	1.617054729423081	4.172960327779776
6	1.004062946815658	3.1092935328047218
7	0.6264112183240372	2.2631174823779774
8	0.43255786663851925	1.7860093752998307
9	0.3329129544213534	1.5464031903359419
>10	1.5304536730779024	13.784039084828242
>50	0.08128873607790167	2.8245810971163166
>100	0.029573241398980533	2.4451046845603712
>500	4.903751334942263E-4	0.14198446622864724
>1k	2.451875655383489E-4	0.3853638091087274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4833	0.15285065039801893	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4045	0.1279290049368894	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	3.162645363087501E-5	0.0	0.0	0.0	0.0
14-15	3.162645363087501E-5	0.0	0.0	0.0	0.0
16-17	3.162645363087501E-5	0.0	0.0	0.0	0.0
18-19	3.162645363087501E-5	0.0	0.0	0.0	0.0
20-21	3.162645363087501E-5	0.0	0.0	0.0	0.0
22-23	3.162645363087501E-5	0.0	0.0	1.5813226815437504E-5	0.0
24-25	3.162645363087501E-5	0.0	0.0	6.325290726175002E-5	0.0
26-27	3.162645363087501E-5	0.0	0.0	1.7394549496981253E-4	0.0
28-29	3.162645363087501E-5	0.0	0.0	8.222877944027503E-4	0.0
30-31	6.325290726175002E-5	0.0	0.0	0.004285384466983564	0.0
32-33	9.487936089262503E-5	0.0	0.0	0.012255250781964065	0.0
34-35	9.487936089262503E-5	0.0	0.0	0.023783093130418008	0.0
36-37	9.487936089262503E-5	0.0	0.0	0.04476724511450358	0.0
38-39	9.487936089262503E-5	0.0	0.0	0.0804102583564997	0.0
40-41	9.487936089262503E-5	0.0	0.0	0.12165115389116073	0.0
42-43	9.487936089262503E-5	0.0	0.0	0.1632557536425768	0.0
44-45	9.487936089262503E-5	0.0	0.0	0.211407029295584	0.0
46-47	9.487936089262503E-5	0.0	0.0	0.27173448959647806	0.0
48-49	9.487936089262503E-5	0.0	0.0	0.33927278132521166	0.0
50-51	9.487936089262503E-5	0.0	0.0	0.41280428601699604	0.0
52-53	9.487936089262503E-5	0.0	0.0	0.48464377543952863	0.0
54-55	9.487936089262503E-5	1.5813226815437504E-5	0.0	0.5617806958452327	0.0
56-57	9.487936089262503E-5	3.162645363087501E-5	0.0	0.6554898779535154	0.0
58-59	9.487936089262503E-5	3.162645363087501E-5	0.0	0.7641899990828328	0.0
60-61	1.2650581452350004E-4	3.162645363087501E-5	0.0	0.8807018542589764	0.0
62-63	1.4231904133893755E-4	3.162645363087501E-5	0.0	0.9971504565278582	0.0
64-65	1.5813226815437504E-4	3.162645363087501E-5	0.0	1.12371952395862	0.0
66-67	1.5813226815437504E-4	3.162645363087501E-5	0.0	1.2632554373780405	0.0
68-69	1.5813226815437504E-4	3.162645363087501E-5	0.0	1.427444171402728	0.0
70-71	1.7394549496981253E-4	3.162645363087501E-5	0.0	1.6092172136461822	0.0
72-73	1.8975872178525005E-4	3.162645363087501E-5	0.0	1.7964458191409622	0.0
74-75	1.8975872178525005E-4	3.162645363087501E-5	0.0	1.9874221593910009	0.0
76-77	2.0557194860068757E-4	3.162645363087501E-5	0.0	2.1940377809615077	0.0
78-79	2.846380826778751E-4	3.162645363087501E-5	0.0	2.423329569785351	0.0
80-81	2.846380826778751E-4	3.162645363087501E-5	0.0	2.680025680680348	0.0
82-83	2.846380826778751E-4	3.162645363087501E-5	0.0	2.945861836674668	0.0
84-85	3.0045130949331257E-4	3.162645363087501E-5	0.0	3.2149713306197834	0.0
86-87	3.162645363087501E-4	3.162645363087501E-5	0.0	3.503230642238394	3.162645363087501E-5
88	3.162645363087501E-4	3.162645363087501E-5	0.0	3.737740795911332	3.162645363087501E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4370	0.0	49.528873	1
GTATCAA	7925	0.0	41.61988	1
ATCAACG	9435	0.0	34.480076	3
TCAACGC	9455	0.0	34.456863	4
CAACGCA	9800	0.0	33.291813	5
AACGCAG	10275	0.0	31.924173	6
TATCAAC	10290	0.0	31.844051	2
ACGCAGA	11790	0.0	27.5828	7
CGCAGAG	11920	0.0	27.242126	8
ACCGTCG	225	3.6379788E-12	27.151888	8
GTACATG	10825	0.0	25.992937	1
TACATGG	10510	0.0	25.765034	2
GCAGAGT	13475	0.0	23.853914	9
ACATGGG	11045	0.0	23.622797	3
ATACCGT	500	0.0	21.617422	6
CATGGGG	7355	0.0	20.965017	4
TAGACCG	135	4.072502E-4	20.893997	5
CCGTCGT	275	1.4424586E-9	20.505995	9
GAGTACT	8275	0.0	20.04652	12-13
AGAGTAC	12510	0.0	19.758808	10-11
>>END_MODULE