FastQCFastQC Report
Wed 25 May 2016
SRR1033185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396784
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.034.999171
TCAACGC38700.031.9281924
GTATCAA41200.031.3421361
ATCAACG40200.030.9778883
CAACGCA41000.030.1366545
GTACATG102000.028.553781
AACGCAG43500.028.3900266
TACATGG101000.027.2780322
TATCAAC50250.025.54252
ACATGGG107850.024.7927153
ACGCAGA53600.022.5147767
GAGTACA71900.022.4167421
CGCAGAG55350.021.2939078
CATGGGG69800.020.9331474
CCGATCG2301.01994374E-720.4160399
AGTACAT77050.020.1362292
TAGACCG1708.6604516E-519.345165
GTGTAGG16200.018.6183091
CTACGCT2152.6335421E-517.4815964
GCAGAGT68400.017.1626039
Produced by FastQC (version 0.11.5)