##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033185_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3396784
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739583382399353	33.0	31.0	34.0	30.0	34.0
2	31.902869302257663	34.0	31.0	34.0	30.0	34.0
3	31.994110605796543	34.0	31.0	34.0	30.0	34.0
4	35.393102416874314	37.0	35.0	37.0	35.0	37.0
5	35.474252410515355	37.0	35.0	37.0	35.0	37.0
6	35.55611337076482	37.0	37.0	37.0	35.0	37.0
7	35.48374079717757	37.0	37.0	37.0	35.0	37.0
8	35.47768448037909	37.0	37.0	37.0	35.0	37.0
9	37.190634729791476	39.0	38.0	39.0	35.0	39.0
10-11	37.17444426846099	39.0	38.0	39.0	35.0	39.0
12-13	37.13141901280741	39.0	38.0	39.0	35.0	39.0
14-15	38.60887842735953	41.0	39.0	41.0	35.0	41.0
16-17	38.6136508827173	41.0	39.0	41.0	35.0	41.0
18-19	38.59953856353539	41.0	39.0	41.0	35.0	41.0
20-21	38.61991813432941	41.0	39.0	41.0	35.0	41.0
22-23	38.576641464396914	41.0	39.0	41.0	34.5	41.0
24-25	38.56097664732288	41.0	39.0	41.0	34.0	41.0
26-27	38.50889458970603	41.0	39.0	41.0	34.0	41.0
28-29	38.4734663728986	40.5	39.0	41.0	34.0	41.0
30-31	38.46532823400016	41.0	39.0	41.0	34.0	41.0
32-33	38.52827115294938	41.0	39.0	41.0	34.0	41.0
34-35	38.49652185714487	41.0	39.0	41.0	34.0	41.0
36-37	38.41916972053566	41.0	39.0	41.0	34.0	41.0
38-39	38.34065074493992	41.0	39.0	41.0	34.0	41.0
40-41	38.27666286699419	41.0	39.0	41.0	34.0	41.0
42-43	38.16423623050509	40.5	38.0	41.0	33.0	41.0
44-45	38.03817979006024	40.0	38.0	41.0	33.0	41.0
46-47	37.909198524251174	40.0	38.0	41.0	33.0	41.0
48-49	37.78136452008724	40.0	38.0	41.0	33.0	41.0
50-51	37.64730300778619	40.0	37.5	41.0	33.0	41.0
52-53	37.488240788934476	40.0	37.0	41.0	32.0	41.0
54-55	37.32252492357477	40.0	37.0	41.0	32.0	41.0
56-57	37.15076407566687	40.0	36.0	41.0	32.0	41.0
58-59	36.937889044460874	40.0	36.0	41.0	31.0	41.0
60-61	36.69974216788586	39.0	35.0	41.0	31.0	41.0
62-63	36.42385768420954	39.0	35.0	41.0	31.0	41.0
64-65	36.14095597482796	38.5	35.0	41.0	31.0	41.0
66-67	35.83751042162233	38.0	35.0	40.0	30.0	41.0
68-69	35.52118680493078	37.0	35.0	40.0	30.0	41.0
70-71	35.16762458254631	37.0	35.0	39.5	30.0	41.0
72-73	34.802929035228615	36.0	34.5	39.0	29.5	41.0
74-75	34.435105234833884	36.0	34.0	39.0	29.0	40.5
76-77	34.10822266002195	35.0	34.0	37.5	29.0	40.0
78-79	33.75140633022294	35.0	34.0	37.0	29.0	39.0
80-81	33.40296571698406	35.0	34.0	37.0	29.0	39.0
82-83	33.19613198837489	35.0	34.0	36.0	29.0	38.5
84-85	32.94206814445664	35.0	34.0	36.0	29.0	37.0
86-87	32.70475897201588	35.0	34.0	36.0	28.0	37.0
88-89	32.361377850343146	35.0	34.0	35.0	27.0	36.5
90-91	32.18471957592829	35.0	34.0	35.0	27.0	36.0
92-93	32.01668622438165	35.0	34.0	35.0	27.0	36.0
94-95	31.85434708241678	35.0	33.5	35.0	26.5	36.0
96-97	31.6823002286869	35.0	33.0	35.0	26.0	36.0
98-99	31.497136703423003	35.0	33.0	35.0	25.0	35.0
100	31.338999477152505	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29008637381504343
1101	2	0.3110319030437019
1101	3	0.2773382170905805
1101	4	0.2788015662771315
1101	5	0.27548628069336445
1101	6	0.2697077784092272
1101	7	0.24679518153472912
1101	8	0.1707771539596905
1101	9	0.2269115766552119
1101	10-11	0.25514286174743006
1101	12-13	0.23594151254761897
1101	14-15	0.29282819143936223
1101	16-17	0.21723855073805964
1101	18-19	0.28739950847065643
1101	20-21	0.2410827130994022
1101	22-23	0.2774093381062528
1101	24-25	0.25111574959504424
1101	26-27	0.2687916174053697
1101	28-29	0.330550274111161
1101	30-31	0.32429716034607736
1101	32-33	0.36376279618638563
1101	34-35	0.33779387993782706
1101	36-37	0.37621512227309495
1101	38-39	0.32591824000987657
1101	40-41	0.3614615854444665
1101	42-43	0.3115597682955311
1101	44-45	0.32680851543170064
1101	46-47	0.2919319808009391
1101	48-49	0.3030646551068088
1101	50-51	0.34202133414591884
1101	52-53	0.3333244747838435
1101	54-55	0.3430402074580172
1101	56-57	0.37557162196331006
1101	58-59	0.31504999915825493
1101	60-61	0.33482621689297787
1101	62-63	0.3380290366805667
1101	64-65	0.3405722508736133
1101	66-67	0.3064224876619761
1101	68-69	0.3038874238548459
1101	70-71	0.350939530432278
1101	72-73	0.4119650984796479
1101	74-75	0.3523615979307664
1101	76-77	0.3270255586549595
1101	78-79	0.36005702516915505
1101	80-81	0.3930979539523989
1101	82-83	0.42113020046883065
1101	84-85	0.36541434397953765
1101	86-87	0.4562844823082628
1101	88-89	0.5626270980845334
1101	90-91	0.5336290059672351
1101	92-93	0.600691538886764
1101	94-95	0.5862563368637055
1101	96-97	0.5649435353583456
1101	98-99	0.6496613423253272
1101	100	0.6857826461749745
1102	1	0.1700141438763758
1102	2	0.16137422193405726
1102	3	0.13146376983741703
1102	4	0.09577218721155845
1102	5	0.031799621237638576
1102	6	0.09722867055743478
1102	7	0.11993281512044973
1102	8	0.12552196393931325
1102	9	0.1419866638543894
1102	10-11	0.07145515780983658
1102	12-13	0.1434903044135183
1102	14-15	0.1725719217862789
1102	16-17	0.19491099318861416
1102	18-19	0.1486657017058235
1102	20-21	0.14021664680894474
1102	22-23	0.1802406372107086
1102	24-25	0.16832348800053865
1102	26-27	0.19414480794598887
1102	28-29	0.19371888060160813
1102	30-31	0.17855653733272447
1102	32-33	0.21670426804211473
1102	34-35	0.17453835046084265
1102	36-37	0.18568832177214034
1102	38-39	0.12595720721817116
1102	40-41	0.19244784749492538
1102	42-43	0.19425867932275054
1102	44-45	0.1596974418148136
1102	46-47	0.1801910002534015
1102	48-49	0.13371572886430272
1102	50-51	0.1679729927128264
1102	52-53	0.09791533416142784
1102	54-55	0.09821014164561603
1102	56-57	0.07176122153507691
1102	58-59	0.06462316762414133
1102	60-61	0.04418457360161199
1102	62-63	0.12181852738518728
1102	64-65	0.13665725161177988
1102	66-67	0.11811998160850123
1102	68-69	0.09087865322005939
1102	70-71	0.09387973685804951
1102	72-73	0.13674061020044093
1102	74-75	0.07842413226559586
1102	76-77	0.08207974617359781
1102	78-79	0.06129327993836853
1102	80-81	0.015323674519351016
1102	82-83	0.0028747161857651804
1102	84-85	0.0625075710167522
1102	86-87	0.05167254247443509
1102	88-89	0.15333507601783936
1102	90-91	0.1641955168655045
1102	92-93	0.15735568473660067
1102	94-95	0.1255092712049617
1102	96-97	0.12201392439771297
1102	98-99	0.14745286575235284
1102	100	0.10273528996949466
1103	1	0.10062288380051498
1103	2	0.06873740400531148
1103	3	0.039913498279979365
1103	4	0.1386941768407155
1103	5	0.01667187387574387
1103	6	0.08565435065811755
1103	7	0.11206754563922772
1103	8	0.05286460061432763
1103	9	0.13575013657425927
1103	10-11	0.12176512628464309
1103	12-13	0.10296793808798554
1103	14-15	0.1372909577102135
1103	16-17	0.12702238070379224
1103	18-19	0.11948404495149134
1103	20-21	0.07691330486424164
1103	22-23	0.07039484025517595
1103	24-25	0.021416715209483073
1103	26-27	0.09296374448493339
1103	28-29	0.06331748550931593
1103	30-31	0.0424346464333496
1103	32-33	0.039382441924807665
1103	34-35	0.016567286832255945
1103	36-37	0.03968470299975735
1103	38-39	-0.017399612033308642
1103	40-41	0.02700241866969577
1103	42-43	-0.031343618672913465
1103	44-45	-0.08784936391079157
1103	46-47	-0.02586878789621494
1103	48-49	-0.00663718037690586
1103	50-51	-0.05237972392354351
1103	52-53	-0.03602128327798937
1103	54-55	-0.05397052996571716
1103	56-57	-0.15970833452087163
1103	58-59	-0.09186679553071997
1103	60-61	-0.04312230101496084
1103	62-63	-0.0181669155582469
1103	64-65	-0.08836206493916166
1103	66-67	-0.05161136673402211
1103	68-69	-0.12875432373715512
1103	70-71	-0.08447179961192575
1103	72-73	-0.08165008749566027
1103	74-75	-0.15864820197890594
1103	76-77	-0.17531413614246816
1103	78-79	-0.20933092817759302
1103	80-81	-0.1063249956871104
1103	82-83	-0.12343455152401361
1103	84-85	-0.08999705490748511
1103	86-87	-0.09996575741227787
1103	88-89	-0.018383356278988572
1103	90-91	0.10510709888519898
1103	92-93	0.04845637788977797
1103	94-95	0.042042884422155424
1103	96-97	0.015234414158413756
1103	98-99	0.10521158913578432
1103	100	0.14483708198168088
1104	1	0.07482750860252096
1104	2	0.05338062769423857
1104	3	0.06546184154095869
1104	4	0.05482247383648797
1104	5	0.0676889248223489
1104	6	0.09464874647466104
1104	7	0.10684643822997941
1104	8	0.08099429361493549
1104	9	0.09091130885417442
1104	10-11	0.1427683073685415
1104	12-13	0.1570704847162716
1104	14-15	0.07479923713000147
1104	16-17	0.10462362019409
1104	18-19	0.09170875578544724
1104	20-21	0.06132806548829706
1104	22-23	0.09906360484280441
1104	24-25	0.14590332737172673
1104	26-27	0.14963362380045453
1104	28-29	0.17582981264544628
1104	30-31	0.14313125777795932
1104	32-33	0.1751776035701269
1104	34-35	0.15544702954127843
1104	36-37	0.209175327144429
1104	38-39	0.1048717102856429
1104	40-41	0.13441943027495995
1104	42-43	0.13154707954304712
1104	44-45	0.14945131424228464
1104	46-47	0.11912019882701941
1104	48-49	0.013819569635707296
1104	50-51	0.06844782072874978
1104	52-53	0.057425278911225064
1104	54-55	0.09387531921284165
1104	56-57	0.08730071577299015
1104	58-59	0.06790443851695471
1104	60-61	0.06785644573038496
1104	62-63	0.0425846485873933
1104	64-65	0.058240839666346744
1104	66-67	0.12151333245237339
1104	68-69	0.059483820835623646
1104	70-71	0.08723932557154512
1104	72-73	0.12814479474450735
1104	74-75	0.13104651640425402
1104	76-77	0.02969742165105771
1104	78-79	0.02812302293382629
1104	80-81	0.12390451557251936
1104	82-83	0.11847677970799708
1104	84-85	0.19345109914243608
1104	86-87	0.1786242649477927
1104	88-89	0.2942754841504609
1104	90-91	0.1945085822339152
1104	92-93	0.23948578766509598
1104	94-95	0.2618229277299591
1104	96-97	0.3060469233418175
1104	98-99	0.30933652050753224
1104	100	0.32058743670456735
1105	1	0.10440096917229269
1105	2	0.1353578140994678
1105	3	0.10435148817968098
1105	4	0.051325724583279
1105	5	0.028487781562162695
1105	6	0.057444212591157395
1105	7	-0.005777837862936508
1105	8	-0.011710500565875748
1105	9	0.010039822816636956
1105	10-11	0.036172258890573517
1105	12-13	0.07280038826905866
1105	14-15	0.07278685836485721
1105	16-17	0.10702009620680286
1105	18-19	0.06601804633089614
1105	20-21	0.08051271437774687
1105	22-23	0.16261583776773136
1105	24-25	0.1816283649417798
1105	26-27	0.15394864047708978
1105	28-29	0.21556975699793668
1105	30-31	0.2278436138925315
1105	32-33	0.18684019272349417
1105	34-35	0.14824646053259016
1105	36-37	0.1378059011624515
1105	38-39	0.15657810147542506
1105	40-41	0.1023349301092793
1105	42-43	0.09121021263791107
1105	44-45	0.09307921156021592
1105	46-47	0.14910087825419538
1105	48-49	0.09552361794492015
1105	50-51	0.099919833750576
1105	52-53	0.1192939555400514
1105	54-55	0.08580739287923222
1105	56-57	0.17171883967451862
1105	58-59	0.1140161988353654
1105	60-61	0.1068612908237867
1105	62-63	0.08811283913404111
1105	64-65	0.10123812528356524
1105	66-67	0.13450057071636934
1105	68-69	0.09874351563037465
1105	70-71	0.08825505850609972
1105	72-73	0.04923114996370259
1105	74-75	0.053818434932630055
1105	76-77	0.12633549042934789
1105	78-79	0.19895864487645554
1105	80-81	0.16171062090629817
1105	82-83	0.14238974660988646
1105	84-85	0.15600195542022277
1105	86-87	0.2069051886161759
1105	88-89	0.21803401344404705
1105	90-91	0.22150487417486175
1105	92-93	0.25431327260328374
1105	94-95	0.2786137424912525
1105	96-97	0.24216347181998898
1105	98-99	0.2728547366087746
1105	100	0.32633593175195585
1106	1	0.09096503482955853
1106	2	0.09825033719350174
1106	3	0.1194004190976301
1106	4	0.15918964907189093
1106	5	0.06416142781515077
1106	6	0.06763502933109322
1106	7	0.023209295910092465
1106	8	0.11385360786977117
1106	9	0.132004508005501
1106	10-11	0.12959143417373298
1106	12-13	0.08735862600109812
1106	14-15	0.1427378142100153
1106	16-17	0.14291795150012376
1106	18-19	0.11878995955621008
1106	20-21	0.1263026926614188
1106	22-23	0.09849809153411115
1106	24-25	0.051768300572902604
1106	26-27	0.11951883854099066
1106	28-29	0.09599613163086218
1106	30-31	0.07941420954372802
1106	32-33	0.09303223745458666
1106	34-35	0.09012825631867116
1106	36-37	0.11676095115024765
1106	38-39	0.11372171353087879
1106	40-41	0.06677175273250668
1106	42-43	0.09339945915965586
1106	44-45	0.0720609351263235
1106	46-47	0.08220429876315194
1106	48-49	0.11061281086787034
1106	50-51	0.03871152065309502
1106	52-53	0.006222392342543515
1106	54-55	0.04367899231254313
1106	56-57	0.06561184842897916
1106	58-59	0.04868379823847846
1106	60-61	0.11265876790589857
1106	62-63	0.16840307786256403
1106	64-65	0.1285256991706305
1106	66-67	0.13747391978543533
1106	68-69	0.11288252523537778
1106	70-71	0.0758146022369246
1106	72-73	0.1307209448036204
1106	74-75	0.11932396215100027
1106	76-77	0.11706239576582078
1106	78-79	0.07885960034415973
1106	80-81	0.1446737534915954
1106	82-83	0.1081421302463994
1106	84-85	0.05170046346130164
1106	86-87	-0.01565990372222359
1106	88-89	0.13548985734825436
1106	90-91	0.12046133333283393
1106	92-93	0.11724077331415117
1106	94-95	0.15161228047718822
1106	96-97	0.12977183323187802
1106	98-99	0.1841321242085776
1106	100	0.18453868617637426
1107	1	0.09317850742351297
1107	2	0.11495716211986817
1107	3	0.09267053755136345
1107	4	0.06652736504808132
1107	5	0.08834371510230454
1107	6	0.035251668293355465
1107	7	0.0312882539399979
1107	8	0.035503338226909875
1107	9	0.0558024812108755
1107	10-11	0.04134708406539289
1107	12-13	0.029114600372743382
1107	14-15	0.06685594717078658
1107	16-17	0.034291771899532364
1107	18-19	0.08316721295375373
1107	20-21	0.05582477074243286
1107	22-23	0.0857190503446219
1107	24-25	0.03843156174497153
1107	26-27	0.1360044793750319
1107	28-29	0.17560866545790077
1107	30-31	0.12214405029823183
1107	32-33	0.1251061373052309
1107	34-35	0.13205633486878554
1107	36-37	0.08661603839364318
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2216	14-15	-0.19356256690429774
2216	16-17	-0.18053453614466264
2216	18-19	-0.1823489154156448
2216	20-21	-0.20342558056154303
2216	22-23	-0.1673115688197413
2216	24-25	-0.18149876230824447
2216	26-27	-0.30138246713350725
2216	28-29	-0.18845781437353537
2216	30-31	-0.2836780326142474
2216	32-33	-0.28661755204247896
2216	34-35	-0.2711430366567029
2216	36-37	-0.2418382241209187
2216	38-39	-0.22006070405337397
2216	40-41	-0.2515120653135341
2216	42-43	-0.2662623445373171
2216	44-45	-0.23148434843445642
2216	46-47	-0.10078493134420796
2216	48-49	-0.07367028692787159
2216	50-51	-0.1044534276098048
2216	52-53	-0.1551456946676879
2216	54-55	-0.15313823367789325
2216	56-57	-0.18397571354233833
2216	58-59	-0.18865491597649253
2216	60-61	-0.1556824422848706
2216	62-63	-0.12258178503927297
2216	64-65	-0.07161231466300677
2216	66-67	-0.11377416412923225
2216	68-69	-0.12217735405164376
2216	70-71	-0.18901261930667346
2216	72-73	-0.12279782865595479
2216	74-75	-0.0994547207191161
2216	76-77	-0.1434014129051775
2216	78-79	-0.16184317306114337
2216	80-81	-0.10071992839243649
2216	82-83	-0.018096744104546758
2216	84-85	-0.08143927860177769
2216	86-87	-0.14032783934034398
2216	88-89	-0.05405235740347081
2216	90-91	-0.06282770768002166
2216	92-93	-0.0013476340487059701
2216	94-95	-0.04938704760071744
2216	96-97	-0.08162261985510355
2216	98-99	-0.07654540074974037
2216	100	-0.11110482372646047
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30734.0
3	5390.0
4	3563.0
5	2926.0
6	2640.0
7	3053.0
8	3505.0
9	4002.0
10	4233.0
11	4699.0
12	5248.0
13	5613.0
14	6182.0
15	6354.0
16	6294.0
17	6567.0
18	6695.0
19	6543.0
20	6666.0
21	7072.0
22	8056.0
23	10581.0
24	15659.0
25	21755.0
26	23283.0
27	21261.0
28	22583.0
29	28218.0
30	35157.0
31	43513.0
32	57612.0
33	79007.0
34	111477.0
35	173519.0
36	333546.0
37	714175.0
38	1107308.0
39	461205.0
40	890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.178255570855804	18.775413032857728	12.57662677378022	24.469704622506253
2	15.792107836808755	19.461918328920895	40.17768243531224	24.568291398958113
3	18.48567442277009	24.610132662127544	29.324628633243073	27.579564281859298
4	12.118369566049727	15.838911033449707	38.51908748570846	33.5236319147921
5	12.947298880992541	38.04495763334621	35.684123748505634	13.323619737155626
6	32.17467522506727	37.40178287254054	17.376603485602086	13.046938416790105
7	28.490795573584478	31.997092316307462	21.894628557834817	17.61748355227324
8	25.745624831429843	36.479789836441306	19.74747159482388	18.027113737304973
9	25.585237601961285	16.22125929952281	20.206457798289943	37.987045300225965
10-11	24.446670897090282	26.319737576320623	28.6361447982721	20.597446728316992
12-13	24.987732607502185	23.612945474182386	28.597003384813934	22.802318533501502
14-15	22.214725102388932	25.640105661786293	25.401023933495853	26.744145302328924
16-17	22.197195034458066	28.92743098706761	26.03858944028042	22.83678453819391
18-19	22.093344245829748	28.076784400018077	27.71578276476312	22.114088589389056
20-21	23.173672090790767	27.418844266977626	27.188466227318504	22.219017414913104
22-23	22.846880583426476	27.13810268616918	27.25461484736524	22.7604018830391
24-25	22.691486963631327	27.25324792061965	27.24266163629208	22.81260347945694
26-27	22.476127630642495	27.2774707081486	27.279001884819593	22.967399776389314
28-29	22.438718083677173	27.363973984769235	27.4020324859121	22.79527544564149
30-31	22.63055167319654	27.746260040496935	27.06204057967771	22.561147706628816
32-33	22.42632167351538	27.414036983197498	27.47184209398793	22.68779924929919
34-35	22.555104383160835	27.185393380009828	27.536446103874212	22.723056132955126
36-37	22.84960589964717	27.50159617230741	27.02762463798135	22.62117329006407
38-39	22.60148008910921	27.71558722155581	27.121805550893598	22.56112713844139
40-41	22.401530911255225	27.37022063158775	27.44426943932098	22.783979017836046
42-43	22.400639960240966	27.340384594435207	27.450263671509845	22.80871177381398
44-45	22.45032043095693	27.518919433766193	27.41483991406889	22.61592022120799
46-47	22.533042069763408	27.62713179333045	27.325082501206033	22.514743635700114
48-49	22.513654783060296	27.446732214335935	27.279786446023273	22.7598265565805
50-51	22.471714109021114	27.415265982840403	27.30123769128187	22.811782216856606
52-53	22.466513273241976	27.40298454730538	27.390648300674663	22.739853878777975
54-55	22.598801527854732	27.769663592860983	27.162552132125168	22.46898274715912
56-57	22.452478207755018	27.48334880678286	27.222594072415156	22.841578913046966
58-59	22.34379225185369	27.11212347503239	27.59070978477306	22.95337448834086
60-61	22.657585759853717	27.578271594325056	27.13127241086476	22.632870234956467
62-63	22.429197355356816	27.558709945233247	27.221395024991335	22.790697674418606
64-65	22.449663327988446	27.327628095887675	27.33731395748849	22.88539461863539
66-67	22.27023422595916	27.354330059586896	27.25794295673473	23.11749275771921
68-69	22.65181699629651	27.475712563225645	27.216781997981254	22.655688442496587
70-71	22.57331215989128	27.250517265540637	27.32158607687208	22.854584497696003
72-73	22.404058038428303	27.32180945413436	27.37774585150653	22.896386655930804
74-75	22.477674296666926	27.360764553494025	27.28212944234281	22.879431707496227
76-77	22.432965628222842	27.312607440324342	27.23856396281869	23.015862968634124
78-79	22.56427324506893	27.4410040126295	27.234815592596295	22.759907149705274
80-81	22.432378603568427	27.273412439696106	27.383138182747974	22.91107077398749
82-83	22.29151336216623	27.29747724150377	27.532602613979428	22.87840678235057
84-85	22.301734457332184	27.262178208027716	27.384061858013204	23.0520254766269
86-87	22.53149557328678	27.267478874763277	27.29789067795799	22.903134873991945
88-89	22.253291483127747	27.284823524969067	27.379334498800844	23.082550493102342
90-91	22.244352742991488	27.12276998552399	27.483944428959006	23.148932842525515
92-93	22.35296870610151	27.32205750432866	27.346879474082293	22.97809431548753
94-95	22.149596247129246	27.04460835801243	27.5582602561196	23.24753513873873
96-97	22.23937143360612	27.00033836086742	27.54843854233842	23.21185166318804
98-99	22.19601459071919	27.131458328582593	27.480500742300023	23.19202633839819
100	22.389381788975584	26.92590861733085	27.380683726360193	23.30402586733337
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	196.0
2	204.5
3	189.0
4	149.5
5	139.5
6	144.0
7	138.5
8	142.5
9	144.5
10	149.0
11	154.0
12	154.0
13	178.5
14	208.0
15	263.0
16	341.5
17	435.5
18	614.5
19	830.0
20	1217.0
21	1878.5
22	2846.0
23	4506.5
24	6986.5
25	10280.5
26	15056.5
27	21005.5
28	28521.0
29	37066.0
30	45457.5
31	54337.5
32	63317.5
33	71958.0
34	80748.5
35	89030.5
36	96143.5
37	102891.5
38	107948.0
39	111324.0
40	115432.0
41	118882.5
42	120075.0
43	120923.5
44	120555.5
45	119170.5
46	118737.5
47	116974.5
48	114464.0
49	113734.5
50	112205.0
51	109057.0
52	104228.5
53	100262.0
54	97818.5
55	93699.5
56	86328.5
57	77754.5
58	71684.5
59	66902.0
60	60556.5
61	52904.5
62	46062.0
63	39349.0
64	33025.0
65	26986.0
66	21295.0
67	16074.0
68	11898.0
69	8988.0
70	6386.5
71	4625.5
72	3467.5
73	2481.0
74	1904.5
75	1425.5
76	949.0
77	666.5
78	464.5
79	315.0
80	212.0
81	122.5
82	83.0
83	55.5
84	36.5
85	29.0
86	25.0
87	22.5
88	15.0
89	9.0
90	10.0
91	8.5
92	5.0
93	6.5
94	5.0
95	3.0
96	4.5
97	6.0
98	5.0
99	3.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3302535574826071
2	0.12076128479173241
3	0.0675933471189219
4	0.04174536856037946
5	0.04542531994969359
6	0.010451061945652122
7	0.007830936556460465
8	0.016839457557501446
9	0.01890023033551736
10-11	0.022050268724770252
12-13	0.017236892307547377
14-15	0.019577341391151158
16-17	0.020593007974601858
18-19	0.019945336530082573
20-21	0.024847031780648992
22-23	0.016839457557501446
24-25	0.025921577586328716
26-27	0.020696046613502654
28-29	0.02050468914125832
30-31	0.021328998252464683
32-33	0.026215973697473843
34-35	0.01679529814082968
36-37	0.011113453195728666
38-39	0.014778684779485539
40-41	0.01105457397349964
42-43	0.009508994389987707
44-45	0.01008306680672071
46-47	0.009376516139972396
48-49	0.00506361311169624
50-51	0.007610139473101616
52-53	0.008508047612094264
54-55	0.003709391000428641
56-57	0.003105878972581124
58-59	0.003341395861497228
60-61	0.0039301880837874885
62-63	0.0031353185836956367
64-65	0.002369888694718298
66-67	0.0018841351113288334
68-69	0.003547473139298819
70-71	0.0022374104447029897
72-73	0.0020313331669013987
74-75	0.002428767916947324
76-77	0.003856589056001206
78-79	0.0039301880837874885
80-81	0.0039301880837874885
82-83	0.0025170867502908633
84-85	0.0030028403336803283
86-87	0.0022521302502602463
88-89	0.6022314047640356
90-91	0.61390421057094
92-93	0.6106511335427863
94-95	0.5969028351523088
96-97	0.59501870004098
98-99	0.5922366567906585
100	0.5937969561797276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3396784.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.841756675585295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.20934650078848	40.32296757000057
2	15.099498548211734	16.863650477051863
3	5.4708854667693405	9.165115651059882
4	2.5418603453215245	5.677677876270551
5	1.3523955787860584	3.7760072419854214
6	0.8237720006681366	2.76005253704821
7	0.5134551296594607	2.0070565489992296
8	0.36588644244841806	1.6345393352080084
9	0.2805298782461321	1.409875308112687
>10	1.2572898321651342	12.254089059951491
>50	0.0663386688016794	2.5239454100442087
>100	0.01868887542871162	1.5892394625561697
>500	5.2732705090377376E-5	0.015783521711652065
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.9439611114513022E-5	0.0
7	0.0	0.0	0.0	2.9439611114513022E-5	0.0
8	0.0	0.0	0.0	2.9439611114513022E-5	0.0
9	0.0	0.0	0.0	5.8879222229026044E-5	0.0
10-11	0.0	4.415941667176953E-5	0.0	5.8879222229026044E-5	0.0
12-13	0.0	8.831883334353906E-5	0.0	7.359902778628255E-5	0.0
14-15	0.0	8.831883334353906E-5	0.0	2.3551688891610418E-4	0.0
16-17	0.0	8.831883334353906E-5	0.0	3.5327533337415625E-4	0.0
18-19	0.0	8.831883334353906E-5	0.0	3.827149444886693E-4	0.0
20-21	0.0	8.831883334353906E-5	0.0	4.121545556031823E-4	0.0
22-23	0.0	8.831883334353906E-5	0.0	4.563139722749518E-4	0.0
24-25	0.0	8.831883334353906E-5	0.0	4.7103377783220835E-4	0.0
26-27	0.0	1.1775844445805209E-4	0.0	6.91830861191056E-4	0.0
28-29	0.0	1.1775844445805209E-4	0.0	0.001295342889038573	0.0
30-31	0.0	1.1775844445805209E-4	0.0	0.004268743611604388	0.0
32-33	0.0	1.1775844445805209E-4	0.0	0.011260651251301232	0.0
34-35	0.0	1.324782500153086E-4	0.0	0.021505635919151764	0.0
36-37	0.0	1.471980555725651E-4	0.0	0.0395668373379055	0.0
38-39	0.0	1.7663766668707813E-4	0.0	0.07430557845303087	0.0
40-41	0.0	1.7663766668707813E-4	0.0	0.11694885515240297	0.0
42-43	0.0	1.7663766668707813E-4	0.0	0.1585028662405381	0.0
44-45	0.0	1.7663766668707813E-4	0.0	0.20685742749612573	0.0
46-47	0.0	1.7663766668707813E-4	0.0	0.26762078483648066	0.0
48-49	0.0	1.7663766668707813E-4	0.0	0.33706882745561684	0.0
50-51	0.0	1.7663766668707813E-4	0.0	0.41493659885350376	0.0
52-53	0.0	1.7663766668707813E-4	0.0	0.4937022783903834	0.0
54-55	0.0	1.7663766668707813E-4	0.0	0.5760890300943481	0.0
56-57	0.0	2.0607727780159115E-4	0.0	0.6746086886890659	0.0
58-59	0.0	2.3551688891610418E-4	0.0	0.7902327613413158	0.0
60-61	0.0	2.3551688891610418E-4	0.0	0.9150272728557365	0.0
62-63	0.0	2.3551688891610418E-4	0.0	1.039291871370096	0.0
64-65	0.0	2.3551688891610418E-4	0.0	1.1678399333016172	0.0
66-67	0.0	2.3551688891610418E-4	0.0	1.318011389596748	0.0
68-69	0.0	2.3551688891610418E-4	0.0	1.4892910470609846	0.0
70-71	0.0	2.3551688891610418E-4	0.0	1.680457161833075	0.0
72-73	0.0	2.3551688891610418E-4	0.0	1.8807053966339926	0.0
74-75	0.0	2.3551688891610418E-4	0.0	2.0796141291292	0.0
76-77	0.0	2.3551688891610418E-4	0.0	2.2888856047367154	0.0
78-79	0.0	2.943961111451302E-4	0.0	2.533381575042746	0.0
80-81	0.0	2.943961111451302E-4	0.0	2.808715537991229	0.0
82-83	0.0	2.943961111451302E-4	0.0	3.0945300024964792	0.0
84-85	0.0	2.943961111451302E-4	0.0	3.3678179124725034	0.0
86-87	0.0	3.385555278168997E-4	0.0	3.659063985228381	0.0
88	0.0	3.5327533337415625E-4	0.0	3.899983042783998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2720	0.0	34.99917	1
TCAACGC	3870	0.0	31.928192	4
GTATCAA	4120	0.0	31.342136	1
ATCAACG	4020	0.0	30.977888	3
CAACGCA	4100	0.0	30.136654	5
GTACATG	10200	0.0	28.55378	1
AACGCAG	4350	0.0	28.390026	6
TACATGG	10100	0.0	27.278032	2
TATCAAC	5025	0.0	25.5425	2
ACATGGG	10785	0.0	24.792715	3
ACGCAGA	5360	0.0	22.514776	7
GAGTACA	7190	0.0	22.416742	1
CGCAGAG	5535	0.0	21.293907	8
CATGGGG	6980	0.0	20.933147	4
CCGATCG	230	1.01994374E-7	20.416039	9
AGTACAT	7705	0.0	20.136229	2
TAGACCG	170	8.6604516E-5	19.34516	5
GTGTAGG	1620	0.0	18.618309	1
CTACGCT	215	2.6335421E-5	17.481596	4
GCAGAGT	6840	0.0	17.162603	9
>>END_MODULE