FastQCFastQC Report
Wed 25 May 2016
SRR1033185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396784
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.11663973923570059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.046.0650631
GTATCAA74050.041.320681
TCAACGC83350.035.870874
ATCAACG84650.035.5421263
CAACGCA86550.034.5989385
AACGCAG90200.033.1886066
TATCAAC93050.032.58622
GTACATG101550.029.2965371
ACGCAGA104200.028.3235727
CGCAGAG106500.027.7539738
TACATGG103750.027.1865122
ACATGGG108300.024.8723353
GCAGAGT121200.023.9225069
CATGGGG71650.021.3890654
GAGTACA71550.020.3295611
CAGAGTA116450.019.97484810-11
AGAGTAC110200.019.80714210-11
GAGTACT76300.019.49247212-13
GTGGTAT22950.019.4858821
CGTATAC2251.8437458E-618.8040073
Produced by FastQC (version 0.11.5)