##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033185_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3396784 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.789705203510145 34.0 31.0 34.0 31.0 34.0 2 32.86932934210712 34.0 31.0 34.0 31.0 34.0 3 32.965418466408224 34.0 31.0 34.0 31.0 34.0 4 36.38292484891591 37.0 37.0 37.0 35.0 37.0 5 36.32257040777394 37.0 37.0 37.0 35.0 37.0 6 36.40814664694605 37.0 37.0 37.0 35.0 37.0 7 36.37956843885275 37.0 37.0 37.0 35.0 37.0 8 36.37380210222375 37.0 37.0 37.0 35.0 37.0 9 38.200006535593666 39.0 39.0 39.0 37.0 39.0 10-11 38.17944326162629 39.0 39.0 39.0 37.0 39.0 12-13 38.14596733262992 39.0 39.0 39.0 37.0 39.0 14-15 39.69795503629315 41.0 40.0 41.0 37.0 41.0 16-17 39.70976709146063 41.0 40.0 41.0 37.0 41.0 18-19 39.71275756715764 41.0 40.0 41.0 37.0 41.0 20-21 39.703764207556326 41.0 40.0 41.0 37.0 41.0 22-23 39.64086456483545 41.0 40.0 41.0 37.0 41.0 24-25 39.596772712071186 41.0 40.0 41.0 37.0 41.0 26-27 39.503365094748446 41.0 39.5 41.0 36.5 41.0 28-29 39.41771422616216 41.0 39.0 41.0 36.0 41.0 30-31 39.33653935604973 41.0 39.0 41.0 36.0 41.0 32-33 39.38863171753046 41.0 39.0 41.0 36.0 41.0 34-35 39.339949934997335 41.0 39.0 41.0 36.0 41.0 36-37 39.26408611792802 41.0 39.0 41.0 35.0 41.0 38-39 39.181177843513154 41.0 39.0 41.0 35.0 41.0 40-41 39.077707619913426 41.0 39.0 41.0 35.0 41.0 42-43 38.96883905482362 41.0 39.0 41.0 35.0 41.0 44-45 38.84028716574265 41.0 39.0 41.0 35.0 41.0 46-47 38.65420394702754 41.0 39.0 41.0 35.0 41.0 48-49 38.48388387368758 41.0 38.0 41.0 35.0 41.0 50-51 38.35403252017202 40.5 38.0 41.0 35.0 41.0 52-53 38.211361099204424 40.0 38.0 41.0 34.0 41.0 54-55 38.06410475320185 40.0 37.5 41.0 34.0 41.0 56-57 37.88984875105394 40.0 37.0 41.0 34.0 41.0 58-59 37.68984677859999 40.0 36.5 41.0 34.0 41.0 60-61 37.48170387048455 40.0 36.0 41.0 33.5 41.0 62-63 37.231258007574226 39.0 35.5 41.0 33.0 41.0 64-65 36.96760303275098 39.0 35.0 41.0 33.0 41.0 66-67 36.6749133886641 38.5 35.0 41.0 33.0 41.0 68-69 36.34597092426248 37.5 35.0 40.5 32.5 41.0 70-71 36.00763987348033 37.0 35.0 40.0 32.0 41.0 72-73 35.65509611444237 36.5 35.0 39.0 32.0 41.0 74-75 35.32472641769391 36.0 35.0 39.0 32.0 41.0 76-77 34.994220562744054 35.5 35.0 38.5 31.0 40.5 78-79 34.666210303628375 35.0 35.0 37.0 31.0 39.0 80-81 34.34604849763777 35.0 35.0 37.0 31.0 39.0 82-83 34.042963579668296 35.0 34.5 36.5 31.0 39.0 84-85 33.79238597449823 35.0 34.0 36.0 31.0 37.0 86-87 33.58102310891714 35.0 34.0 36.0 31.0 37.0 88-89 33.38065461330482 35.0 34.0 35.0 31.0 37.0 90-91 33.201245501627426 35.0 34.0 35.0 30.0 36.0 92-93 33.056061262653145 35.0 34.0 35.0 30.0 36.0 94-95 32.92039676352691 35.0 34.0 35.0 30.0 36.0 96-97 32.8089644499032 35.0 34.0 35.0 30.0 36.0 98-99 32.71522887531265 35.0 34.0 35.0 30.0 35.0 100 32.62471649654497 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12459731791685869 1101 2 0.16079062787716936 1101 3 0.17150313287619667 1101 4 0.09236664079136858 1101 5 0.06957277411722629 1101 6 0.11441056295453222 1101 7 0.1641943592733881 1101 8 0.11933980185569482 1101 9 0.17884536364775272 1101 10-11 0.1778892488377295 1101 12-13 0.19880538110578527 1101 14-15 0.2634161421290955 1101 16-17 0.2818978250815647 1101 18-19 0.26836895909956837 1101 20-21 0.24621763986240808 1101 22-23 0.2691452771133811 1101 24-25 0.2233563020109699 1101 26-27 0.2617010124307626 1101 28-29 0.2533254436984862 1101 30-31 0.2389685568551556 1101 32-33 0.21455437640459962 1101 34-35 0.24881665521043317 1101 36-37 0.28334916063656124 1101 38-39 0.23170119908655806 1101 40-41 0.2614556057723405 1101 42-43 0.20543604898930568 1101 44-45 0.1522293130345247 1101 46-47 0.2419278534253806 1101 48-49 0.2086963107404003 1101 50-51 0.17933924839596216 1101 52-53 0.23698822330300828 1101 54-55 0.21900124530948517 1101 56-57 0.2661193098617787 1101 58-59 0.23822705967982927 1101 60-61 0.26326103376196386 1101 62-63 0.2487946155373706 1101 64-65 0.2983800162331818 1101 66-67 0.3154912874735629 1101 68-69 0.31826708617963817 1101 70-71 0.25001550096519765 1101 72-73 0.3268520985149834 1101 74-75 0.28737125630636484 1101 76-77 0.3129975101301312 1101 78-79 0.24545576852810314 1101 80-81 0.21100772607062623 1101 82-83 0.23749412735535458 1101 84-85 0.23216981630522326 1101 86-87 0.2045504523809072 1101 88-89 0.30924351055067234 1101 90-91 0.21961809884368932 1101 92-93 0.21351103502317414 1101 94-95 0.18068295758397568 1101 96-97 0.2661090807198718 1101 98-99 0.3281744252053116 1101 100 0.31504795275316155 1102 1 0.09686983930613735 1102 2 0.13408603848408518 1102 3 0.0843350948249153 1102 4 0.07922324246916901 1102 5 0.01033608950355358 1102 6 0.05407867883979378 1102 7 0.0504773998002932 1102 8 0.09010736547116949 1102 9 0.12322871259809887 1102 10-11 0.10028173499583204 1102 12-13 0.11147188306568978 1102 14-15 0.1420649240523275 1102 16-17 0.09593435583502696 1102 18-19 0.12574521059473653 1102 20-21 0.11788119819119913 1102 22-23 0.18386031935965974 1102 24-25 0.14978661448177633 1102 26-27 0.17300039809464351 1102 28-29 0.11932369218273209 1102 30-31 0.125475809476157 1102 32-33 0.13685647470841644 1102 34-35 0.12201103228245813 1102 36-37 0.12338374905546345 1102 38-39 0.13686400267675936 1102 40-41 0.11480106112124133 1102 42-43 0.08352492837072845 1102 44-45 0.1250095128720261 1102 46-47 0.18853022419837373 1102 48-49 0.1408552045537519 1102 50-51 0.1341709311466417 1102 52-53 0.15529291875117224 1102 54-55 0.1473638492429643 1102 56-57 0.10995043923793446 1102 58-59 0.11736795325415983 1102 60-61 0.1544046811529185 1102 62-63 0.12924035821922075 1102 64-65 0.08100425684966694 1102 66-67 0.07151513580675584 1102 68-69 0.10704447997642319 1102 70-71 0.15961107557586018 1102 72-73 0.1356935873435532 1102 74-75 0.15529099992624396 1102 76-77 0.1488257993190416 1102 78-79 0.164915419892111 1102 80-81 0.13889504053700108 1102 82-83 0.0965209615006728 1102 84-85 0.10697485472508816 1102 86-87 0.03166786319069104 1102 88-89 0.08020319857197933 1102 90-91 0.1189953265276813 1102 92-93 0.0365642563960904 1102 94-95 0.0411409327554324 1102 96-97 -0.027601983456513324 1102 98-99 0.05145050285450026 1102 100 0.0780002811944982 1103 1 0.1015938468975861 1103 2 0.13424161746589647 1103 3 0.08848155172467642 1103 4 0.044616813099587205 1103 5 0.040272598943538185 1103 6 0.07382718352481987 1103 7 0.0728902715029065 1103 8 0.10802427723629648 1103 9 0.13775657062423363 1103 10-11 0.08865053510415066 1103 12-13 0.08664399726136907 1103 14-15 0.15210436363203428 1103 16-17 0.16259084698547355 1103 18-19 0.13969178016393613 1103 20-21 0.13415531815525128 1103 22-23 0.10681605089506974 1103 24-25 0.15370091218581194 1103 26-27 0.18580327745792147 1103 28-29 0.15576463872384494 1103 30-31 0.17019777492856747 1103 32-33 0.12790822072630448 1103 34-35 0.16100799462262216 1103 36-37 0.14126361297090284 1103 38-39 0.12663952173032555 1103 40-41 0.17124824225479074 1103 42-43 0.14974645358035588 1103 44-45 0.1938591927500255 1103 46-47 0.19827336892802805 1103 48-49 0.24398232449877355 1103 50-51 0.1795698718463825 1103 52-53 0.14281940246698355 1103 54-55 0.09822912211015478 1103 56-57 0.11246863764739601 1103 58-59 0.13798359749031874 1103 60-61 0.1396737085657307 1103 62-63 0.07806283244304524 1103 64-65 0.15050639663233056 1103 66-67 0.15565656299370545 1103 68-69 0.14808054716841923 1103 70-71 0.1798981042859893 1103 72-73 0.24923689161861518 1103 74-75 0.1913976583328889 1103 76-77 0.18782858817563408 1103 78-79 0.18188065265088937 1103 80-81 0.18337075322000373 1103 82-83 0.13049027778997413 1103 84-85 0.1436815563754763 1103 86-87 0.1715589385549876 1103 88-89 0.17002079606751863 1103 90-91 0.09380663644246567 1103 92-93 0.09448246802404725 1103 94-95 0.07087063384282999 1103 96-97 0.15316199465321034 1103 98-99 0.15133854139447322 1103 100 0.1472647210941176 1104 1 0.07516347157494607 1104 2 0.07812295501344835 1104 3 0.11947880608402528 1104 4 0.05229381869305172 1104 5 -0.03161556650378827 1104 6 0.004578307803029702 1104 7 0.057868160294034965 1104 8 0.08420805027967049 1104 9 0.11296948731035883 1104 10-11 0.09946465912005209 1104 12-13 0.13860155201018642 1104 14-15 0.14863919513515356 1104 16-17 0.1549495327636521 1104 18-19 0.12996651949568871 1104 20-21 0.15351480309685428 1104 22-23 0.1806829179098699 1104 24-25 0.14940678074065517 1104 26-27 0.11427004759978843 1104 28-29 0.12345103638051569 1104 30-31 0.10013941974862206 1104 32-33 0.08979755688465474 1104 34-35 0.10014918620054658 1104 36-37 0.0844055350520918 1104 38-39 0.14852715967705166 1104 40-41 0.16347575479760934 1104 42-43 0.1362838936590478 1104 44-45 0.17122326966948265 1104 46-47 0.2205062432592655 1104 48-49 0.21362819547501744 1104 50-51 0.19242148822310412 1104 52-53 0.17909576803204885 1104 54-55 0.17745392763897883 1104 56-57 0.2201462090277957 1104 58-59 0.25262551689145596 1104 60-61 0.2932070990563105 1104 62-63 0.23372519836323846 1104 64-65 0.22595197937203437 1104 66-67 0.24384483748902142 1104 68-69 0.22526341486761225 1104 70-71 0.20098017648873423 1104 72-73 0.23578945479935953 1104 74-75 0.20742134004719048 1104 76-77 0.17846449526099661 1104 78-79 0.13971065894430978 1104 80-81 0.16554986843302544 1104 82-83 0.16467697522864455 1104 84-85 0.11233247187404771 1104 86-87 0.13751857999410788 1104 88-89 0.1158133448832217 1104 90-91 0.14679851778273445 1104 92-93 0.1599101974206576 1104 94-95 0.1688787787925108 1104 96-97 0.16708160832536834 1104 98-99 0.20252945483918694 1104 100 0.1800069814903651 1105 1 0.05427001488813232 1105 2 0.05005491808932305 1105 3 0.07490210357374139 1105 4 0.05909185870142153 1105 5 0.02822123466142301 1105 6 0.016128749980779844 1105 7 -0.024810948215332473 1105 8 -0.014848284939027678 1105 9 0.037272244823448375 1105 10-11 0.012266719708094342 1105 12-13 0.041414667553929974 1105 14-15 0.04510128163413896 1105 16-17 0.07240636867067707 1105 18-19 0.07372450402857567 1105 20-21 0.027880290008468478 1105 22-23 0.0873507683046526 1105 24-25 0.05004998747242695 1105 26-27 0.032413712053411814 1105 28-29 0.06261070819701331 1105 30-31 0.03043751443786391 1105 32-33 0.012523232193352385 1105 34-35 -0.009769308588801096 1105 36-37 0.029704700830961883 1105 38-39 -0.019624962903115772 1105 40-41 0.08521549517203653 1105 42-43 0.05940056788274006 1105 44-45 0.086142319459384 1105 46-47 0.10757784324599129 1105 48-49 0.05420246335555845 1105 50-51 0.07110672262789564 1105 52-53 0.07169167151356248 1105 54-55 0.16271819187003445 1105 56-57 0.04045326974076602 1105 58-59 0.002718965877896551 1105 60-61 -0.006879489222164636 1105 62-63 -0.0316199935502226 1105 64-65 -0.10756474990115095 1105 66-67 -0.06793026727500262 1105 68-69 -0.08610176771528444 1105 70-71 -0.04233243545856169 1105 72-73 -0.08873350071990416 1105 74-75 -0.08528357701197109 1105 76-77 -0.08264878670099307 1105 78-79 -0.06064689657929989 1105 80-81 -0.0378403452892897 1105 82-83 -0.05669249171278068 1105 84-85 0.009057620444217207 1105 86-87 -0.035637558660184254 1105 88-89 -0.013070194701420235 1105 90-91 -0.0358316646629433 1105 92-93 -0.022906575802011275 1105 94-95 -0.050170785829010356 1105 96-97 -0.015302162065772507 1105 98-99 0.007013122053486143 1105 100 -0.0327677386644325 1106 1 0.0033959519233874857 1106 2 0.03787868122795146 1106 3 0.039072442051384826 1106 4 0.02812534410770695 1106 5 4.323988121370803E-4 1106 6 0.00990172763285102 1106 7 0.028883468199488505 1106 8 0.08009958834637843 1106 9 0.08791832418489776 1106 10-11 0.03980618276393244 1106 12-13 0.053831218257634816 1106 14-15 0.09698642419937897 1106 16-17 0.08713798466173728 1106 18-19 0.07096932469908523 1106 20-21 0.08123877031908933 1106 22-23 0.09007977733089234 1106 24-25 0.0840560318059218 1106 26-27 0.10440343517663564 1106 28-29 0.03735535901219578 1106 30-31 0.09480287200221227 1106 32-33 0.08818032069679305 1106 34-35 0.1197294042852306 1106 36-37 0.14425387961188108 1106 38-39 0.08690251465710475 1106 40-41 0.14812286488041337 1106 42-43 0.17087372948184054 1106 44-45 0.18242412329786362 1106 46-47 0.25622617224696853 1106 48-49 0.23864547626205024 1106 50-51 0.21729755949883156 1106 52-53 0.2457491002309382 1106 54-55 0.23451969011989604 1106 56-57 0.23458860543780702 1106 58-59 0.23158279726061437 1106 60-61 0.2001963065186274 1106 62-63 0.23061600722482467 1106 64-65 0.21072023966847553 1106 66-67 0.29743660766365565 1106 68-69 0.24323463895109398 1106 70-71 0.16350631017866846 1106 72-73 0.2930119578517534 1106 74-75 0.24785079491333306 1106 76-77 0.19116228887330067 1106 78-79 0.20679472980439328 1106 80-81 0.21600253726895602 1106 82-83 0.26016671960486804 1106 84-85 0.26533408956702687 1106 86-87 0.25010365226994224 1106 88-89 0.16261029609242428 1106 90-91 0.18763152202442512 1106 92-93 0.16470933473581084 1106 94-95 0.17658407140444154 1106 96-97 0.17219245918199277 1106 98-99 0.1386260934649428 1106 100 0.18309448475447 1107 1 0.027103497195298587 1107 2 0.06359295899578399 1107 3 0.03372223081492365 1107 4 0.007328884833185612 1107 5 -0.06214313780821357 1107 6 -0.06361887081947515 1107 7 -0.07442238940571855 1107 8 -0.001022111112852997 1107 9 -0.01399438328528646 1107 10-11 -0.021924352377716616 1107 12-13 -0.007926517576734682 1107 14-15 0.03579374706344396 1107 16-17 0.04677095145630972 1107 18-19 0.03213661823016167 1107 20-21 -0.005452692798201042 1107 22-23 -0.003783360724206375 1107 24-25 -0.008832182988697923 1107 26-27 0.06788981489793855 1107 28-29 0.049025346190603614 1107 30-31 0.06665603276164234 1107 32-33 0.1251137995207685 1107 34-35 0.1294325421919993 1107 36-37 0.14039716626027143 1107 38-39 0.1212036821360627 1107 40-41 0.15936871037787625 1107 42-43 0.09715649616497757 1107 44-45 0.15306697375245903 1107 46-47 0.1828131342707593 1107 48-49 0.12402001422574216 1107 50-51 0.11701765380198026 1107 52-53 0.12469656136055818 1107 54-55 0.10032689895241731 1107 56-57 0.12441246843680176 1107 58-59 0.15760618392300785 1107 60-61 0.12868179160227555 1107 62-63 0.12696668293119018 1107 64-65 0.09100615288027569 1107 66-67 0.08392209089313951 1107 68-69 0.06799361059061937 1107 70-71 0.09056834796853508 1107 72-73 0.11110140122026024 1107 74-75 0.06314168193960512 1107 76-77 0.05540368714095223 1107 78-79 0.020556110939494943 1107 80-81 0.046581548606290823 1107 82-83 0.03500949051552027 1107 84-85 -0.006138762120592389 1107 86-87 0.060076201264102735 1107 88-89 0.03145580561405836 1107 90-91 0.027712159933507508 1107 92-93 0.06697301828810964 1107 94-95 0.08037025208044923 1107 96-97 0.11635670538256448 1107 98-99 0.08773450593276522 1107 100 0.15159526675120816 1108 1 0.018717180384179244 1108 2 0.04179316672961875 1108 3 -0.028104124137485087 1108 4 -0.012922098497028855 1108 5 -0.07866240426941573 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-0.08106003362088643 2214 18-19 -0.09490284305419294 2214 20-21 -0.09372245072005114 2214 22-23 -0.1323825909414893 2214 24-25 -0.12479001357696973 2214 26-27 -0.183373696805873 2214 28-29 -0.1349913787278041 2214 30-31 -0.0952054328014924 2214 32-33 -0.07471233804152178 2214 34-35 -0.10087462690558624 2214 36-37 -0.11118809676536046 2214 38-39 -0.13928151574902614 2214 40-41 -0.13855800154548348 2214 42-43 -0.1430102900913539 2214 44-45 -0.1569896522316725 2214 46-47 -0.1394770023921268 2214 48-49 -0.14856084106634881 2214 50-51 -0.07598431694759711 2214 52-53 -0.09941329470197502 2214 54-55 -0.1550758316599996 2214 56-57 -0.13943358493877156 2214 58-59 -0.24863201555255898 2214 60-61 -0.2802735623225203 2214 62-63 -0.2449313539244713 2214 64-65 -0.1272239089867071 2214 66-67 -0.23838122267758877 2214 68-69 -0.2153859769407518 2214 70-71 -0.11951927764194892 2214 72-73 -0.22985222995197319 2214 74-75 -0.22259022337183154 2214 76-77 -0.13950126387137374 2214 78-79 -0.3483143949413403 2214 80-81 -0.22660060023820705 2214 82-83 -0.17451081713304006 2214 84-85 -0.22007597919733257 2214 86-87 -0.19659420740190114 2214 88-89 -0.33852202345318005 2214 90-91 -0.2201931911806554 2214 92-93 -0.21973100223943476 2214 94-95 -0.2959809017669741 2214 96-97 -0.40544558809379083 2214 98-99 -0.4716415335880413 2214 100 -0.5584310870644771 2215 1 -0.033744935760267936 2215 2 -0.0792300318361967 2215 3 -0.05990069742122728 2215 4 -0.06207523043719476 2215 5 -0.0818108447656769 2215 6 -0.05769114305619638 2215 7 -0.07034785496220053 2215 8 -0.06636142186141569 2215 9 -0.05365059271860417 2215 10-11 -0.09092143259038465 2215 12-13 -0.0774555488251849 2215 14-15 -0.13931750242877428 2215 16-17 -0.1285170258470245 2215 18-19 -0.1254927674306927 2215 20-21 -0.1378833650790341 2215 22-23 -0.12170589270930066 2215 24-25 -0.10726292500062584 2215 26-27 -0.0743425210373374 2215 28-29 -0.05549965119448075 2215 30-31 -0.1092455782701407 2215 32-33 -0.1672814234120139 2215 34-35 -0.14816697260923206 2215 36-37 -0.09984664936095555 2215 38-39 -0.08671884174076894 2215 40-41 -0.17923144578736583 2215 42-43 -0.14785848386029699 2215 44-45 -0.09920033314305954 2215 46-47 -0.14466327269619228 2215 48-49 -0.07604522489924648 2215 50-51 -0.06929119499422143 2215 52-53 -0.109639926899888 2215 54-55 -0.04561404528782731 2215 56-57 -0.12544050433806575 2215 58-59 -0.13121210897844549 2215 60-61 -0.1432541465932573 2215 62-63 -0.09694529695381249 2215 64-65 -0.01629243976763206 2215 66-67 -0.07821974055380565 2215 68-69 -0.06674840458967424 2215 70-71 -0.06325777346575023 2215 72-73 -0.03038970928002982 2215 74-75 -0.06333529990354947 2215 76-77 -0.10644254928479313 2215 78-79 -0.13287656489437438 2215 80-81 -0.04287374605222993 2215 82-83 0.014592806618779264 2215 84-85 2.2600129231165056E-4 2215 86-87 -0.08537216569016692 2215 88-89 -0.1012825754404929 2215 90-91 -0.049622263151057666 2215 92-93 -0.03227917236322497 2215 94-95 0.004587978538289406 2215 96-97 -0.033246613818057824 2215 98-99 -0.05031519612892765 2215 100 -0.04543597197323379 2216 1 -0.04374584437648821 2216 2 -0.0640789823676684 2216 3 -0.09345534010579826 2216 4 -0.02225642631385938 2216 5 -0.05480680436264862 2216 6 -0.03581339442915521 2216 7 -0.0696577433098966 2216 8 -0.06520146661033976 2216 9 -0.13144090525739216 2216 10-11 -0.13856903192052528 2216 12-13 -0.12306619350770376 2216 14-15 -0.20583748887846554 2216 16-17 -0.16020119555234658 2216 18-19 -0.11530471914336005 2216 20-21 -0.16274342390563135 2216 22-23 -0.15997664824971736 2216 24-25 -0.16572444292044253 2216 26-27 -0.2300172827859086 2216 28-29 -0.2753155380047474 2216 30-31 -0.2140968246950905 2216 32-33 -0.2247265843924353 2216 34-35 -0.19283998749693865 2216 36-37 -0.20557199802353665 2216 38-39 -0.19671762577377905 2216 40-41 -0.209764813584286 2216 42-43 -0.23025977177760382 2216 44-45 -0.10265794535673933 2216 46-47 -0.10550065407433351 2216 48-49 -0.17655988628686714 2216 50-51 -0.1671951802924454 2216 52-53 -0.07652766778605269 2216 54-55 -0.1293319449769399 2216 56-57 -0.12425753976687304 2216 58-59 -0.06789722005048304 2216 60-61 -0.09971610420942056 2216 62-63 -0.07108785967815123 2216 64-65 -0.039279632309785484 2216 66-67 -0.007506977317525809 2216 68-69 -0.00454703239444143 2216 70-71 -0.04129516747381956 2216 72-73 0.01429124130385162 2216 74-75 0.04373634591281217 2216 76-77 0.0388393056493328 2216 78-79 0.02217266245433791 2216 80-81 -0.01928386047909214 2216 82-83 -0.009376774724117354 2216 84-85 -0.07882360965704294 2216 86-87 -0.0900816444719652 2216 88-89 -0.07273388746719434 2216 90-91 -0.09777959449987605 2216 92-93 -0.10382392406326346 2216 94-95 -0.13283161690411305 2216 96-97 -0.08166846837261943 2216 98-99 -0.052503558820980345 2216 100 -0.04958739076653984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26.0 3 9.0 4 11.0 5 26.0 6 62.0 7 136.0 8 304.0 9 600.0 10 1127.0 11 1699.0 12 2756.0 13 4184.0 14 4943.0 15 6679.0 16 8101.0 17 11581.0 18 13647.0 19 7547.0 20 5477.0 21 5277.0 22 5486.0 23 5877.0 24 6893.0 25 8597.0 26 10924.0 27 14193.0 28 18386.0 29 23874.0 30 30973.0 31 39528.0 32 51782.0 33 68225.0 34 96475.0 35 152793.0 36 300147.0 37 705567.0 38 1216960.0 39 564061.0 40 1851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86008336677787 18.91366829734583 12.741102721000638 24.485145614875663 2 15.97684612334191 19.678439968993917 39.80654466191684 24.538169245747333 3 18.602654746371865 24.686703658519352 29.625404500256714 27.085237094852072 4 12.315633784074999 16.044556420036436 38.125078751237936 33.514731044650624 5 12.78020157255098 37.8960754551168 35.694810369718525 13.628912602613694 6 32.03916536175321 37.60084927565159 17.066059973910562 13.293925388684627 7 28.27341399778957 32.06426855858556 21.917893719112694 17.744423724512178 8 25.960656118008686 36.10079820017093 19.573818280735768 18.364727401084618 9 25.786112604356248 15.621892275562313 20.316399835373403 38.27559528470804 10-11 24.563316832856387 26.020774732941188 28.57767399946508 20.83823443473735 12-13 25.360856033236146 23.36904554425598 28.75656797723965 22.51353044526823 14-15 22.338288025718477 25.277807217423558 25.958687345073866 26.425217411784104 16-17 21.887541274334783 28.742113128182424 26.482932091060253 22.887413506422547 18-19 21.731175978686895 27.496100354938726 28.90538946624064 21.867334200133744 20-21 22.779532051493412 26.823342314377363 28.567727591745605 21.82939804238362 22-23 22.399233155429396 26.554951944151767 28.65857841124101 22.38723648917783 24-25 22.265642422287808 26.71484071461197 28.60037615029879 22.41914071280143 26-27 22.025687027597687 26.73033861350344 28.667140258848644 22.576834100050224 28-29 22.06565195156054 26.822728896062316 28.733335355030064 22.37828379734708 30-31 22.257150505856377 27.14612362315268 28.362487847990696 22.234238023000245 32-33 22.04890346269591 26.831310572221838 28.786612776103617 22.333173188978634 34-35 22.177962962199487 26.677688208932633 28.781199808938997 22.363149019928887 36-37 22.47604666016662 26.992263844967113 28.255856446928373 22.275833047937894 38-39 22.26768711138857 27.21053739724757 28.26189189626918 22.259883595094678 40-41 22.190980132543945 26.89719127462229 28.450683569592638 22.46114502324113 42-43 22.1940501358439 26.861741637609143 28.36931224986149 22.574895976685468 44-45 22.27287122421309 27.066305794367867 28.275143583074414 22.385679398344628 46-47 22.390640697525143 27.24514727480658 28.01074483634235 22.353467191325926 48-49 22.443447640302555 27.136049534655708 27.801104695840607 22.619398129201127 50-51 22.45366717385365 27.196503354029073 27.63860395605845 22.71122551605883 52-53 22.403595819480064 27.218542246963224 27.757123203098615 22.620738730458097 54-55 22.523968433650165 27.52515342299998 27.556566681043552 22.394311462306298 56-57 22.3190778094589 27.258408541243767 27.666555283886247 22.755958365411082 58-59 22.20050215551218 26.87661008376668 28.07788842090977 22.844999339811377 60-61 22.55129700707659 27.245825988717197 27.64539763966424 22.557479364541972 62-63 22.268204280256736 27.221312934139263 27.74135636996169 22.76912641564231 64-65 22.32611433615 27.044301416145032 27.809665067657125 22.819919180047847 66-67 22.146223474589732 27.073762650450067 27.736628349343412 23.043385525616788 68-69 22.52901229970186 27.168875298115935 27.674756068228767 22.627356333953436 70-71 22.528768117382015 26.989311177006996 27.671429275878793 22.810491429732195 72-73 22.33890171234757 27.132161896766917 27.684215840963013 22.844720549922506 74-75 22.442616399267834 27.19946243008978 27.526081782164958 22.831839388477427 76-77 22.440512674673087 27.122675809459214 27.479587985541578 22.957223530326125 78-79 22.522839993016923 27.29958022046154 27.462749313100073 22.71483047342147 80-81 22.462554044116132 27.175203914787083 27.54423183019593 22.81801021090085 82-83 22.36544666817693 27.176654621387726 27.643319021192653 22.814579689242695 84-85 22.34210719610619 27.07751132729005 27.512747755260175 23.067633721343586 86-87 22.509996607078325 27.16130214593096 27.42789305452006 22.90080819247066 88-89 22.20900710789971 27.202362587671164 27.486027960565053 23.102602343864078 90-91 22.220303740333133 27.053391400039388 27.557795125564393 23.168509734063083 92-93 22.313624050517202 27.19725881852641 27.405102503589056 23.08401462736733 94-95 22.07604310430101 26.91887385244396 27.634506639221097 23.370576404033933 96-97 22.15277745067099 26.8784974258004 27.627470572164732 23.34125455136388 98-99 22.078633790079085 27.06352538165512 27.48168561792566 23.376155210340134 100 22.246748689348514 26.845716418824395 27.42664826494708 23.48088662688001 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27.0 1 24.5 2 74.0 3 145.0 4 113.5 5 83.0 6 204.0 7 453.5 8 773.5 9 931.0 10 788.5 11 655.0 12 742.0 13 897.5 14 1055.5 15 1227.5 16 1414.0 17 1641.5 18 1895.0 19 2218.5 20 2661.5 21 3304.5 22 4376.5 23 6070.5 24 8354.0 25 11463.5 26 16097.0 27 21916.5 28 29184.5 29 37353.5 30 45350.5 31 54001.0 32 62871.0 33 71663.0 34 80070.5 35 87924.5 36 95142.0 37 101795.5 38 107143.0 39 110363.5 40 113913.0 41 117819.5 42 119480.5 43 120422.5 44 120265.0 45 118830.0 46 118618.0 47 117112.0 48 114525.5 49 112927.0 50 111188.0 51 108773.5 52 103880.0 53 99454.5 54 97406.0 55 93438.5 56 85994.5 57 77577.5 58 71567.5 59 66893.0 60 60373.5 61 52818.0 62 45782.0 63 38765.0 64 32443.5 65 26387.5 66 20750.5 67 15645.5 68 11478.0 69 8550.5 70 6023.5 71 4340.0 72 3216.5 73 2337.0 74 1753.5 75 1271.0 76 830.0 77 562.0 78 398.5 79 255.0 80 142.0 81 56.5 82 20.5 83 14.0 84 10.5 85 3.0 86 3.0 87 4.0 88 2.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09143943212167745 2 9.126279445499037E-4 3 0.0 4 3.5327533337415625E-4 5 0.0377415814488057 6 2.0607727780159115E-4 7 0.00476921700055111 8 5.004733889467214E-4 9 1.7663766668707813E-4 10-11 3.974347500459258E-4 12-13 0.0 14-15 1.1775844445805209E-4 16-17 0.0 18-19 1.4719805557256511E-5 20-21 0.0 22-23 2.0607727780159115E-4 24-25 1.0303863890079558E-4 26-27 0.0014719805557256512 28-29 0.008743564501010367 30-31 0.03694671194871384 32-33 0.026186534086359334 34-35 0.02973400722565815 36-37 0.026819485725321366 38-39 0.025877418169656944 40-41 0.03179478000367406 42-43 0.023272012586022545 44-45 0.02218274697478556 46-47 0.016132906890753137 48-49 0.01101041455682787 50-51 0.008007574223147542 52-53 0.005093052722810753 54-55 0.00379770983377218 56-57 0.002620125389191659 58-59 8.095893056491081E-4 60-61 6.3295163896203E-4 62-63 0.0014278211390538816 64-65 0.0028850818892222764 66-67 5.151931945039779E-4 68-69 0.0010303863890079557 70-71 3.3855552781689977E-4 72-73 4.268743611604388E-4 74-75 4.8575358338946484E-4 76-77 2.9439611114513025E-4 78-79 2.9439611114513022E-5 80-81 6.476714445192866E-4 82-83 5.151931945039779E-4 84-85 2.796763055878737E-4 86-87 1.9135747224433467E-4 88-89 0.0 90-91 2.9439611114513022E-5 92-93 1.4719805557256511E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3396784.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.00373776764783 #Duplication Level Percentage of deduplicated Percentage of total 1 70.73900751901472 38.201708120005385 2 15.745888009029041 17.006736139167092 3 5.81924541669278 9.427830104659902 4 2.663713924116574 5.754020329840945 5 1.4554296666094135 3.929932102741493 6 0.8813288501782611 2.855703126725363 7 0.5571514068631475 2.1061780931179435 8 0.4057840181414617 1.7531082964811155 9 0.2830572401718808 1.3757534074328723 >10 1.3550662333866381 12.738912080641946 >50 0.07153912590375039 2.6129565169556987 >100 0.022406938101495473 1.8938181480868246 >500 1.635650577815184E-4 0.04578502856297588 >1k 2.1808673294574916E-4 0.2975585055803322 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3962 0.11663973923570059 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.9439611114513022E-5 0.0 0.0 0.0 12-13 0.0 7.359902778628255E-5 0.0 1.4719805557256511E-5 0.0 14-15 0.0 8.831883334353906E-5 0.0 1.324782500153086E-4 0.0 16-17 0.0 8.831883334353906E-5 0.0 2.0607727780159115E-4 0.0 18-19 0.0 8.831883334353906E-5 0.0 2.3551688891610415E-4 0.0 20-21 0.0 8.831883334353906E-5 0.0 2.649565000306172E-4 0.0 22-23 0.0 8.831883334353906E-5 0.0 3.0911591670238674E-4 0.0 24-25 0.0 8.831883334353906E-5 0.0 3.385555278168997E-4 0.0 26-27 0.0 8.831883334353906E-5 0.0 5.740724167330039E-4 0.0 28-29 0.0 8.831883334353906E-5 0.0 0.0011628646390232644 0.0 30-31 0.0 8.831883334353906E-5 0.0 0.00409210594491731 0.0 32-33 0.0 8.831883334353906E-5 0.0 0.011069293779056896 0.0 34-35 0.0 1.0303863890079558E-4 0.0 0.02137315766913645 0.0 36-37 0.0 1.1775844445805209E-4 0.0 0.03934604025454666 0.0 38-39 0.0 1.1775844445805209E-4 0.0 0.07409950117522927 0.0 40-41 0.0 1.471980555725651E-4 0.0 0.11686053631905943 0.0 42-43 0.0 1.471980555725651E-4 0.0 0.15863534449055341 0.0 44-45 0.0 1.471980555725651E-4 0.0 0.20743149991285875 0.0 46-47 0.0 1.471980555725651E-4 0.0 0.26865117122548854 0.0 48-49 0.0 1.471980555725651E-4 0.0 0.33834945053909815 0.0 50-51 0.0 1.471980555725651E-4 0.0 0.4168060141592753 0.0 52-53 0.0 1.471980555725651E-4 0.0 0.4957777709739565 0.0 54-55 0.0 1.471980555725651E-4 0.0 0.5786944356779824 0.0 56-57 0.0 1.471980555725651E-4 0.0 0.6782003212450365 0.0 58-59 0.0 1.471980555725651E-4 0.0 0.7943984663140193 0.0 60-61 0.0 1.471980555725651E-4 0.0 0.9201792048007763 0.0 62-63 0.0 1.471980555725651E-4 0.0 1.0458716244541897 0.0 64-65 0.0 1.471980555725651E-4 0.0 1.1751998360802451 0.0 66-67 0.0 1.471980555725651E-4 0.0 1.3257392875143077 0.0 68-69 0.0 1.471980555725651E-4 0.0 1.498078770978667 0.0 70-71 0.0 1.471980555725651E-4 0.0 1.6907904653342691 0.0 72-73 0.0 1.471980555725651E-4 0.0 1.8921279657464236 0.0 74-75 0.0 1.471980555725651E-4 0.0 2.0920082054084097 0.0 76-77 0.0 1.471980555725651E-4 0.0 2.302913579432781 0.0 78-79 0.0 1.471980555725651E-4 0.0 2.550765665405866 0.0 80-81 0.0 1.471980555725651E-4 0.0 2.829779579743663 0.0 82-83 0.0 1.471980555725651E-4 0.0 3.119774469027174 0.0 84-85 0.0 1.471980555725651E-4 0.0 3.3985234268649407 0.0 86-87 0.0 1.471980555725651E-4 0.0 3.694538716621369 0.0 88 0.0 1.471980555725651E-4 0.0 3.939284923621873 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4200 0.0 46.065063 1 GTATCAA 7405 0.0 41.32068 1 TCAACGC 8335 0.0 35.87087 4 ATCAACG 8465 0.0 35.542126 3 CAACGCA 8655 0.0 34.598938 5 AACGCAG 9020 0.0 33.188606 6 TATCAAC 9305 0.0 32.5862 2 GTACATG 10155 0.0 29.296537 1 ACGCAGA 10420 0.0 28.323572 7 CGCAGAG 10650 0.0 27.753973 8 TACATGG 10375 0.0 27.186512 2 ACATGGG 10830 0.0 24.872335 3 GCAGAGT 12120 0.0 23.922506 9 CATGGGG 7165 0.0 21.389065 4 GAGTACA 7155 0.0 20.329561 1 CAGAGTA 11645 0.0 19.974848 10-11 AGAGTAC 11020 0.0 19.807142 10-11 GAGTACT 7630 0.0 19.492472 12-13 GTGGTAT 2295 0.0 19.485882 1 CGTATAC 225 1.8437458E-6 18.804007 3 >>END_MODULE