FastQCFastQC Report
Wed 25 May 2016
SRR1033184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177242
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC32150.037.3991974
ATCAACG33350.036.3403133
GTATCAA37100.035.284991
CAACGCA34700.034.7856455
GGTATCA20900.034.021211
AACGCAG36600.032.8453036
TATCAAC43650.028.3215332
GTACATG78300.026.461231
ACGCAGA44350.026.1531837
CGCAGAG44850.025.2334048
TACATGG81150.024.154212
ACATGGG81650.022.7826293
TACACCG3600.020.8743975
CTGTCGC7300.018.6550489
GCAGAGT57000.018.6189359
CATGGGG51500.018.057654
TCTGTCG6900.017.6947948
TAGACTG14650.017.6328095
GTATAGA15900.017.473261
GAGTACA60800.016.883871
Produced by FastQC (version 0.11.5)