##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033184_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3177242
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.716878663948165	33.0	31.0	34.0	30.0	34.0
2	31.827781767961017	34.0	31.0	34.0	30.0	34.0
3	31.98617543139616	34.0	31.0	34.0	30.0	34.0
4	35.30821700078244	37.0	35.0	37.0	33.0	37.0
5	35.4228384240168	37.0	35.0	37.0	35.0	37.0
6	35.49067398706173	37.0	37.0	37.0	35.0	37.0
7	35.43409598639323	37.0	37.0	37.0	35.0	37.0
8	35.42373322523119	37.0	37.0	37.0	35.0	37.0
9	37.09728878064686	39.0	38.0	39.0	35.0	39.0
10-11	37.08716112905469	39.0	38.0	39.0	35.0	39.0
12-13	37.03945764911832	39.0	38.0	39.0	35.0	39.0
14-15	38.52010328454679	41.0	39.0	41.0	35.0	41.0
16-17	38.54284234565702	41.0	39.0	41.0	35.0	41.0
18-19	38.54671646037664	41.0	39.0	41.0	35.0	41.0
20-21	38.5876793457974	41.0	39.0	41.0	35.0	41.0
22-23	38.557919415644136	41.0	39.0	41.0	34.0	41.0
24-25	38.563403731915926	41.0	39.0	41.0	34.0	41.0
26-27	38.52077902784868	41.0	39.0	41.0	34.0	41.0
28-29	38.49183096534668	40.5	39.0	41.0	34.0	41.0
30-31	38.4927156949329	41.0	39.0	41.0	34.0	41.0
32-33	38.555321092947906	41.0	39.0	41.0	34.0	41.0
34-35	38.52844920216968	41.0	39.0	41.0	34.0	41.0
36-37	38.455548082267576	41.0	39.0	41.0	34.0	41.0
38-39	38.387211613090855	41.0	39.0	41.0	34.0	41.0
40-41	38.335013039611084	41.0	39.0	41.0	34.0	41.0
42-43	38.233008376447245	41.0	38.5	41.0	33.0	41.0
44-45	38.13361084865427	40.5	38.0	41.0	33.0	41.0
46-47	38.02802776747883	40.0	38.0	41.0	33.0	41.0
48-49	37.9238575468913	40.0	38.0	41.0	33.0	41.0
50-51	37.81795610784448	40.0	38.0	41.0	33.0	41.0
52-53	37.68813407980884	40.0	38.0	41.0	32.5	41.0
54-55	37.55244879049188	40.0	37.0	41.0	32.0	41.0
56-57	37.41105729434522	40.0	37.0	41.0	32.0	41.0
58-59	37.2358581751091	40.0	37.0	41.0	31.5	41.0
60-61	37.03400181666993	40.0	36.0	41.0	31.0	41.0
62-63	36.79312403650714	39.5	36.0	41.0	31.0	41.0
64-65	36.539332540612264	39.0	35.0	41.0	31.0	41.0
66-67	36.267641086199916	39.0	35.0	41.0	31.0	41.0
68-69	35.9756145424239	38.0	35.0	40.5	30.5	41.0
70-71	35.63604566476208	37.5	35.0	40.0	30.0	41.0
72-73	35.276525049083446	37.0	35.0	39.5	30.0	41.0
74-75	34.90408127552135	36.5	35.0	39.0	30.0	41.0
76-77	34.56323660583613	36.0	34.0	39.0	29.0	40.5
78-79	34.17922540996248	35.5	34.0	38.5	29.0	39.5
80-81	33.798584590031226	35.0	34.0	37.0	29.0	39.0
82-83	33.548187862303216	35.0	34.0	37.0	29.0	39.0
84-85	33.25199858871311	35.0	34.0	36.5	29.0	38.0
86-87	32.97356968716893	35.0	34.0	36.0	29.0	37.0
88-89	32.58678092509163	35.0	34.0	36.0	28.0	37.0
90-91	32.37961146805941	35.0	34.0	35.0	27.0	36.5
92-93	32.1900467449442	35.0	34.0	35.0	27.0	36.0
94-95	32.01073855878778	35.0	34.0	35.0	27.0	36.0
96-97	31.83143241843083	35.0	34.0	35.0	26.5	36.0
98-99	31.641763044804264	35.0	33.5	35.0	26.0	36.0
100	31.483912147705464	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21589913212514844
1101	2	0.23047138348718121
1101	3	0.2443208587751471
1101	4	0.23490835746954275
1101	5	0.15375204515349594
1101	6	0.12118931501065333
1101	7	0.17396688820036843
1101	8	0.13458387599751376
1101	9	0.1451717244839088
1101	10-11	0.156403623308762
1101	12-13	0.20403388925948462
1101	14-15	0.19622275309505
1101	16-17	0.1334622108657726
1101	18-19	0.16277137814436315
1101	20-21	0.1325443804599189
1101	22-23	0.17504674541041965
1101	24-25	0.15971385939401017
1101	26-27	0.18035715450044165
1101	28-29	0.2076637505755059
1101	30-31	0.23913535360442495
1101	32-33	0.24550325295260222
1101	34-35	0.2677774230940173
1101	36-37	0.18697782014181286
1101	38-39	0.16728626954387948
1101	40-41	0.19947125909760643
1101	42-43	0.2002966655569267
1101	44-45	0.10095675998321951
1101	46-47	0.10791053038658305
1101	48-49	0.18373476080154205
1101	50-51	0.1523638062296655
1101	52-53	0.1767872038373426
1101	54-55	0.19277961354679718
1101	56-57	0.2392798248483814
1101	58-59	0.2200073643353022
1101	60-61	0.2588751860563505
1101	62-63	0.2522133117398937
1101	64-65	0.24311270930714812
1101	66-67	0.25326316149248385
1101	68-69	0.22202272539730927
1101	70-71	0.23990993367277014
1101	72-73	0.27748013341663125
1101	74-75	0.2463531335011524
1101	76-77	0.22713129199541982
1101	78-79	0.14301224809287305
1101	80-81	0.19187548432402934
1101	82-83	0.12100542411628368
1101	84-85	0.10990732643473677
1101	86-87	0.1872806470122299
1101	88-89	0.2656847842683092
1101	90-91	0.3230418462373663
1101	92-93	0.3927118817302784
1101	94-95	0.350412047638585
1101	96-97	0.35211844160586026
1101	98-99	0.4384510415339946
1101	100	0.41813135224153797
1102	1	0.13644134117568996
1102	2	0.11745500769241346
1102	3	0.10748125469383041
1102	4	0.050375119082630704
1102	5	0.03098967564941546
1102	6	0.07295515587001944
1102	7	0.0857002069568722
1102	8	0.06752767293824746
1102	9	0.08549021849977123
1102	10-11	0.0487027941527316
1102	12-13	0.08831978927322126
1102	14-15	0.12334790557318343
1102	16-17	0.1416171380086766
1102	18-19	0.11329424826426049
1102	20-21	0.15310008293290878
1102	22-23	0.09986654173854248
1102	24-25	0.11619284437346522
1102	26-27	0.17131370518769273
1102	28-29	0.17870425595834405
1102	30-31	0.17862604585147324
1102	32-33	0.1355200655599873
1102	34-35	0.1740974417681258
1102	36-37	0.14571577147586368
1102	38-39	0.0941903564257558
1102	40-41	0.17230944292234085
1102	42-43	0.19037072671758892
1102	44-45	0.1645633991410307
1102	46-47	0.16332454453695533
1102	48-49	0.14351397098327112
1102	50-51	0.10614637203841681
1102	52-53	0.11744488014196719
1102	54-55	0.17222639800021255
1102	56-57	0.08232770827703462
1102	58-59	0.08777633035009558
1102	60-61	0.09306510548486102
1102	62-63	0.14532551053248
1102	64-65	0.14813270567279346
1102	66-67	0.08419377920068172
1102	68-69	0.10426966586226172
1102	70-71	0.17502410329296936
1102	72-73	0.16533524065240357
1102	74-75	0.05414620054130381
1102	76-77	0.09488449926867304
1102	78-79	0.10412834126446313
1102	80-81	0.037814062659002445
1102	82-83	0.03793036178307574
1102	84-85	0.02487655502956443
1102	86-87	0.01699616725507269
1102	88-89	0.013291474642031176
1102	90-91	0.01888786658880548
1102	92-93	0.05879830660860108
1102	94-95	0.12090294228696763
1102	96-97	0.10902037643460005
1102	98-99	0.10281927112310285
1102	100	0.1753654271713998
1103	1	0.06695181326055177
1103	2	0.10858086885505713
1103	3	0.12934594745934191
1103	4	0.10551368373904069
1103	5	0.07873115127747354
1103	6	0.1251943511125333
1103	7	0.11590333875133751
1103	8	0.043425651272983146
1103	9	0.031164475832063943
1103	10-11	0.08256224594247641
1103	12-13	0.07803303105828263
1103	14-15	0.11664069766236906
1103	16-17	0.08478880801930444
1103	18-19	0.07213130988471761
1103	20-21	0.06414379172923645
1103	22-23	0.053523895949233236
1103	24-25	0.048799516560706024
1103	26-27	0.12453326531566944
1103	28-29	0.07584751523948086
1103	30-31	0.07347446434590665
1103	32-33	0.10725961826063468
1103	34-35	0.12334535318792916
1103	36-37	0.1199812823889772
1103	38-39	0.10254826723454613
1103	40-41	0.08412365408820932
1103	42-43	0.10806510631553579
1103	44-45	0.01609956565398818
1103	46-47	0.11594297587230074
1103	48-49	0.11001414085611572
1103	50-51	0.028132769371609356
1103	52-53	-0.03352448877503633
1103	54-55	0.058849285258609996
1103	56-57	0.03375455703218222
1103	58-59	0.03097229847914207
1103	60-61	0.04831622130819113
1103	62-63	0.04515478177675192
1103	64-65	0.027579588250347342
1103	66-67	0.032929623228632465
1103	68-69	-0.058190169134022085
1103	70-71	0.005398439344510564
1103	72-73	0.027703137464804684
1103	74-75	0.019346016902680674
1103	76-77	0.016924105201688633
1103	78-79	-0.048987678446962946
1103	80-81	-0.07433414064729504
1103	82-83	-0.03592179581613664
1103	84-85	-0.014421657299379831
1103	86-87	-0.032581813419128025
1103	88-89	0.08722710650128818
1103	90-91	0.07079723032759233
1103	92-93	0.105544248858493
1103	94-95	0.09877070869313087
1103	96-97	0.1219268855011002
1103	98-99	0.14347855848448887
1103	100	0.24771005532578627
1104	1	0.20874412575522427
1104	2	0.23200179713810698
1104	3	0.21917770504722256
1104	4	0.2830370302322507
1104	5	0.239488698076606
1104	6	0.2557625945688997
1104	7	0.2933207485435787
1104	8	0.26803875445666137
1104	9	0.3261364030643108
1104	10-11	0.359309488164655
1104	12-13	0.3302008747306999
1104	14-15	0.27446322991775673
1104	16-17	0.3335949362078452
1104	18-19	0.3204389905520273
1104	20-21	0.31571460216972724
1104	22-23	0.31856607162617934
1104	24-25	0.32033486087765084
1104	26-27	0.3329879300640286
1104	28-29	0.3712676925765095
1104	30-31	0.3398366809337432
1104	32-33	0.31474960866221124
1104	34-35	0.2799777807281174
1104	36-37	0.3445881795964425
1104	38-39	0.33731750774168745
1104	40-41	0.32854939358294644
1104	42-43	0.30492171753283515
1104	44-45	0.32609721471111186
1104	46-47	0.3056776225276039
1104	48-49	0.22807676973604174
1104	50-51	0.2705988860010038
1104	52-53	0.2667792880355222
1104	54-55	0.2612331083533874
1104	56-57	0.2658465943616548
1104	58-59	0.22432237283406664
1104	60-61	0.2349031327074158
1104	62-63	0.27692955861007107
1104	64-65	0.2296635779629952
1104	66-67	0.25066329872881
1104	68-69	0.15771679673306238
1104	70-71	0.20480215724180084
1104	72-73	0.22252918074551786
1104	74-75	0.2483801109787791
1104	76-77	0.14248337014480228
1104	78-79	0.12554056316455586
1104	80-81	0.21390954655812777
1104	82-83	0.26434827178045595
1104	84-85	0.24800040560100456
1104	86-87	0.2542287970872579
1104	88-89	0.28480008431599657
1104	90-91	0.24557359648623134
1104	92-93	0.28795991983238167
1104	94-95	0.32101308316283905
1104	96-97	0.34255043909071503
1104	98-99	0.35708418348973225
1104	100	0.37865684043087455
1105	1	0.10945804413834992
1105	2	0.10642159712763544
1105	3	0.11273724585288392
1105	4	0.08949699104668696
1105	5	0.05287486215082993
1105	6	0.07227120754611605
1105	7	0.03904214493108071
1105	8	0.029426109720581906
1105	9	0.09592880465397258
1105	10-11	0.11831207324650705
1105	12-13	0.1519073353082021
1105	14-15	0.19501271800469766
1105	16-17	0.17928407716594563
1105	18-19	0.13855389531315865
1105	20-21	0.15790136119530018
1105	22-23	0.23949028665021643
1105	24-25	0.19687742830258514
1105	26-27	0.18894453547368073
1105	28-29	0.2207704414826921
1105	30-31	0.26691539097396344
1105	32-33	0.18886928747714649
1105	34-35	0.2098200321052559
1105	36-37	0.23418018639894456
1105	38-39	0.18955227096144966
1105	40-41	0.22794737714045255
1105	42-43	0.21063804278410458
1105	44-45	0.21400378818709243
1105	46-47	0.2141635666142534
1105	48-49	0.18095513110660733
1105	50-51	0.2539444175291905
1105	52-53	0.23605384653654227
1105	54-55	0.14479887323248164
1105	56-57	0.18478416004109022
1105	58-59	0.2069059826786841
1105	60-61	0.22796785788739982
1105	62-63	0.2724441636432786
1105	64-65	0.20888382865265953
1105	66-67	0.22095641916141773
1105	68-69	0.21881020216459035
1105	70-71	0.1962348059568484
1105	72-73	0.16378030441241975
1105	74-75	0.19407536071659592
1105	76-77	0.2705214267252387
1105	78-79	0.27453542088585436
1105	80-81	0.26799695921866373
1105	82-83	0.2522036423317999
1105	84-85	0.23245995153679644
1105	86-87	0.29285281925797335
1105	88-89	0.33125824779963864
1105	90-91	0.2656644434696602
1105	92-93	0.274330526225512
1105	94-95	0.2980695209586557
1105	96-97	0.37391149308625415
1105	98-99	0.4039830549876342
1105	100	0.5131264544578826
1106	1	0.11942470978323527
1106	2	0.1272201963504216
1106	3	0.10726140087772507
1106	4	0.07144222419753987
1106	5	0.02596591699924744
1106	6	0.05670850759496915
1106	7	0.042180575850700563
1106	8	0.08443667131476218
1106	9	0.046068243328228675
1106	10-11	0.007860697364421299
1106	12-13	0.03250748272197512
1106	14-15	0.050814182548066356
1106	16-17	0.020698281293498155
1106	18-19	0.03242416149441851
1106	20-21	0.07376398028708309
1106	22-23	0.03529997827453002
1106	24-25	0.02463133129524664
1106	26-27	0.09524783809985138
1106	28-29	0.11631619283313199
1106	30-31	0.10169158077474094
1106	32-33	0.12768525457782687
1106	34-35	0.15941212440927188
1106	36-37	0.09962506963259443
1106	38-39	0.12849996574219347
1106	40-41	0.09318718949269567
1106	42-43	0.06969990270881254
1106	44-45	0.06810593318564884
1106	46-47	0.12652950312207878
1106	48-49	0.10263128130031163
1106	50-51	0.0714279776549418
1106	52-53	0.07274774024310915
1106	54-55	0.15398365878718323
1106	56-57	0.022194968387083236
1106	58-59	0.039798542422218475
1106	60-61	0.07122127051105309
1106	62-63	0.09686515067478041
1106	64-65	0.059095688326237905
1106	66-67	0.05302012279738477
1106	68-69	-0.054651764060125174
1106	70-71	0.01739606869298882
1106	72-73	0.05156340960250816
1106	74-75	0.046204881000100784
1106	76-77	0.013183686218852131
1106	78-79	0.017375388271943848
1106	80-81	0.04376878167857967
1106	82-83	-0.011735505844505667
1106	84-85	0.01661413557160074
1106	86-87	0.04166092729259674
1106	88-89	0.1402043389014196
1106	90-91	0.0809982338341797
1106	92-93	0.10926483253824415
1106	94-95	0.13187982322789082
1106	96-97	0.11608572154954189
1106	98-99	0.10728358996870568
1106	100	0.09095298627004311
1107	1	0.04872197243170717
1107	2	-0.005416358411920896
1107	3	0.03138463700931027
1107	4	0.04574359249205173
1107	5	0.005426311733771172
1107	6	-0.03281945123582375
1107	7	-0.06315096397344178
1107	8	0.0017092473367128491
1107	9	-0.027152381927471936
1107	10-11	-0.06446898582520788
1107	12-13	-0.06318163268473853
1107	14-15	-0.10842244603965412
1107	16-17	-0.0702359347443604
1107	18-19	-0.05610435471422903
1107	20-21	-0.0767458535758152
1107	22-23	-0.03466988032099749
1107	24-25	-0.010605911795629197
1107	26-27	0.060205846009644404
1107	28-29	-0.007876943656690116
1107	30-31	0.008710229245316725
1107	32-33	0.006624884143427323
1107	34-35	-0.06023343877423315
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2216	12-13	-0.18403162021667185
2216	14-15	-0.21993898609902374
2216	16-17	-0.19781041948792222
2216	18-19	-0.23446495657699273
2216	20-21	-0.2139683240109207
2216	22-23	-0.19177600063670042
2216	24-25	-0.20506259837188168
2216	26-27	-0.27648036475392246
2216	28-29	-0.19857214707898407
2216	30-31	-0.2514668206277264
2216	32-33	-0.20012225367861447
2216	34-35	-0.2065501436165107
2216	36-37	-0.1930560795941716
2216	38-39	-0.1648319129749538
2216	40-41	-0.17336656711961496
2216	42-43	-0.18958788386066772
2216	44-45	-0.15700093828765915
2216	46-47	-0.04949010794086206
2216	48-49	-0.057823615959684105
2216	50-51	-0.09642248855595881
2216	52-53	-0.06730516538478781
2216	54-55	-0.026417662876475845
2216	56-57	-0.07938647025774515
2216	58-59	-0.12284386218206578
2216	60-61	-0.012352728994237339
2216	62-63	0.021428910112199162
2216	64-65	0.040901461530381766
2216	66-67	-0.005748276013100906
2216	68-69	-0.047126747094949906
2216	70-71	-0.1267252354322892
2216	72-73	-0.01176022767280216
2216	74-75	-0.03904362467034872
2216	76-77	-0.017983653453001125
2216	78-79	-0.0897571690221497
2216	80-81	-0.04647657200911226
2216	82-83	-0.021161294220256366
2216	84-85	-0.02833144854223235
2216	86-87	-0.050242522860074246
2216	88-89	-0.05407545840747474
2216	90-91	-0.009378755827817997
2216	92-93	0.018734365025125044
2216	94-95	0.046361802378072525
2216	96-97	0.008213992139538107
2216	98-99	-0.06455867727695619
2216	100	-0.020424364367638503
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29184.0
3	5221.0
4	3209.0
5	2699.0
6	2181.0
7	2721.0
8	3156.0
9	3310.0
10	3751.0
11	4164.0
12	4510.0
13	5047.0
14	5821.0
15	6072.0
16	5791.0
17	5960.0
18	6181.0
19	5912.0
20	6002.0
21	6360.0
22	7256.0
23	10119.0
24	15576.0
25	22207.0
26	24659.0
27	20718.0
28	20070.0
29	25663.0
30	31343.0
31	37716.0
32	49237.0
33	67917.0
34	94504.0
35	141592.0
36	262672.0
37	581761.0
38	1081792.0
39	563547.0
40	1641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4665099630176	20.31812061718166	13.473598255966696	23.741771163834045
2	14.890649041195946	20.33133464982391	41.540571258597126	23.23744505038302
3	17.829571619015766	25.92044609595182	30.33734652145747	25.912635763574944
4	11.633479984244447	17.058157324427437	40.30534413636173	31.00301855496638
5	12.191246751333	38.55285306484613	36.66842153985084	12.587478643970027
6	30.00151718511169	39.34687384322505	18.219315718037755	12.432293253625506
7	26.42321871034024	32.93144779516932	23.420880673233818	17.224452821256623
8	24.058497392151807	37.62805905519371	20.93770470955128	17.375738843103203
9	24.382870822886122	17.243447178426567	21.710808594493948	36.66287340419336
10-11	23.05380201606061	27.518037418100093	29.837111545049392	19.591049020789896
12-13	23.77763514432351	24.864279616413242	29.71102245312193	21.647062786141316
14-15	21.036450282284637	27.0176044438995	26.614446356474918	25.331498917340944
16-17	20.78371135049391	30.392507306852224	27.189092631321476	21.63468871133239
18-19	20.65683324985968	29.46755875920899	29.102866509415104	20.772741481516228
20-21	21.879646440049726	28.751899501682832	28.521645425722912	20.846808632544526
22-23	21.433556307547022	28.560053513811283	28.59096560950657	21.415424569135123
24-25	21.347724771381582	28.70738353745917	28.507759552106865	21.437132139052384
26-27	21.1323491080524	28.581499847008352	28.609501096125346	21.676649948813907
28-29	21.11964926634317	28.65592444632413	28.6832015621461	21.5412247251866
30-31	21.403088432136215	29.117904268299593	28.28689730116337	21.19210999840082
32-33	20.99704105407608	28.837224603797484	28.769271792043305	21.396462550083125
34-35	21.157495823613086	28.557247585533695	28.83263572116373	21.452620869689486
36-37	21.47146060115979	28.903473415930144	28.34163110068465	21.283434882225425
38-39	21.296388834808507	29.044931379472565	28.48480203144273	21.1738777542762
40-41	21.072295417839307	28.687474248107698	28.791520549105098	21.448709784947894
42-43	21.055017160156584	28.624560766249346	28.748609332474867	21.571812741119203
44-45	21.178962053079605	28.79182766943535	28.681801241688603	21.34740903579644
46-47	21.205003773210755	28.92902980088936	28.56338639960151	21.302580026298372
48-49	21.178759014671343	28.77217914065051	28.584650370422832	21.464411474255318
50-51	21.180858510888765	28.739649157264825	28.601533001199858	21.477959330646552
52-53	21.198395290434586	28.67841550432346	28.644925037539064	21.478264167702882
54-55	21.297783625091377	29.091189502533325	28.4002867351925	21.210740137182803
56-57	21.173992051705305	28.77230431774042	28.484043687451006	21.569659943103265
58-59	20.985544733300777	28.456006041899546	28.887871553924256	21.67057767087542
60-61	21.28948707348122	28.846917415914326	28.488150396041206	21.375445114563245
62-63	21.14903113005189	28.828212661957004	28.502008872717887	21.520747335273224
64-65	21.115135827489436	28.578042716269664	28.672025622774456	21.634795833466445
66-67	21.008442373709826	28.66823702854128	28.521865411132552	21.801455186616337
68-69	21.42838932477892	28.73236785649538	28.405030668976494	21.43421214974921
70-71	21.334595458866037	28.547800250536636	28.557793389106063	21.55981090149126
72-73	21.090668340682857	28.727763956163145	28.643302516264768	21.53826518688923
74-75	21.155147198954293	28.685316646415476	28.57622617796697	21.583309976663266
76-77	21.151843254514695	28.696840697171183	28.43681000904906	21.714506039265057
78-79	21.26676651573898	28.75140240629235	28.545965326866696	21.43586575110198
80-81	21.239114786264714	28.507354591296362	28.634450389360715	21.619080233078208
82-83	21.046822877741565	28.57764703271821	28.820535724262005	21.554994365278215
84-85	21.082404122676895	28.503011652863453	28.621246213991807	21.793338010467842
86-87	21.232790911920755	28.651666119534568	28.466737568272173	21.648805400272504
88-89	21.008008118143685	28.526647157581113	28.580029334867884	21.88531538940731
90-91	21.002736350427647	28.475412823497877	28.69667358393509	21.825177242139386
92-93	21.19212301831064	28.604990114509572	28.529614041576057	21.673272825603735
94-95	20.88815326035372	28.372040895346323	28.73592057744589	22.00388526685407
96-97	20.926864510638676	28.31813733668881	28.777357305428293	21.97764084724422
98-99	20.97445785405831	28.351305536529743	28.677228643686583	21.997007965725363
100	21.038118590113957	28.342939093518048	28.55951587601842	22.059426440349576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	344.0
1	340.5
2	315.0
3	277.0
4	241.0
5	210.0
6	191.5
7	181.5
8	197.0
9	203.5
10	204.0
11	231.5
12	249.5
13	282.0
14	336.0
15	392.5
16	516.5
17	809.5
18	1145.0
19	1600.0
20	2413.0
21	3652.0
22	5507.0
23	8584.5
24	12763.5
25	17543.0
26	23838.0
27	32095.0
28	42061.0
29	52683.0
30	63118.5
31	73700.5
32	83803.0
33	93074.0
34	101485.0
35	108479.0
36	113513.5
37	118310.0
38	121546.0
39	122063.5
40	121614.5
41	120915.0
42	119105.5
43	115667.5
44	111019.5
45	106619.0
46	103719.0
47	100154.0
48	96231.5
49	92856.0
50	89478.5
51	85298.0
52	79024.0
53	73281.0
54	69429.0
55	65643.5
56	60729.5
57	55235.0
58	50187.5
59	44832.5
60	39277.0
61	33607.0
62	28572.0
63	23884.5
64	19256.5
65	15356.5
66	12014.0
67	8960.5
68	6785.0
69	5291.5
70	3808.5
71	2784.5
72	2143.5
73	1646.0
74	1302.0
75	967.5
76	666.5
77	486.0
78	348.5
79	225.5
80	158.0
81	96.5
82	51.5
83	32.5
84	31.5
85	26.5
86	17.5
87	13.0
88	10.5
89	12.0
90	10.5
91	5.0
92	3.0
93	3.5
94	4.0
95	3.0
96	2.5
97	1.5
98	2.5
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3251247465569195
2	0.11497393021998326
3	0.061720196321211916
4	0.0383666085239966
5	0.04167136151416858
6	0.009630994428501197
7	0.006861296684357062
8	0.01526481143079438
9	0.01753092776691231
10-11	0.02048946853906627
12-13	0.01542218062080257
14-15	0.018018772255937697
16-17	0.01890003971998356
18-19	0.018601038258967997
20-21	0.02363685233923006
22-23	0.015485128296805845
24-25	0.024234855261261182
26-27	0.018821355124979462
28-29	0.018459405987960628
30-31	0.01932493653300567
32-33	0.024329276775266098
34-35	0.014257648614741969
36-37	0.008907096154463525
38-39	0.012164638387633049
40-41	0.00915888685847663
42-43	0.008198934799426672
44-45	0.008749726964455336
46-47	0.007537984201392276
48-49	0.004075862021212108
50-51	0.006483610628337407
52-53	0.00687703360335788
54-55	0.0029742776911547814
56-57	0.002596591635135127
58-59	0.0030057515291564193
60-61	0.003477859099180988
62-63	0.002738223906142497
64-65	0.002155957903112196
66-67	0.0017310610900900846
68-69	0.002832645420147411
70-71	0.0019513779561015497
72-73	0.0016523764950859895
74-75	0.001967114875102369
76-77	0.003682439046191634
78-79	0.0035722806131859015
80-81	0.0034935960181818066
82-83	0.0020930102271089204
84-85	0.0026123285541359456
86-87	0.0021716948221130153
88-89	0.5943676937419309
90-91	0.6044078480644535
92-93	0.6004578813952478
94-95	0.5867667618645354
96-97	0.5862002327805059
98-99	0.5829426905473364
100	0.583745273416378
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3177242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.35086394056434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42373820077171	52.14555657330049
2	14.080012024449347	18.966019482803954
3	4.285771138074502	8.659511664025601
4	1.6922407217798217	4.558954984291007
5	0.8230205590386208	2.771557284604867
6	0.488918477426329	1.9757449110701135
7	0.30367052739457806	1.4316730661318149
8	0.1831208908842333	0.9866680165295141
9	0.12155433270356561	0.7368110390973545
>10	0.5747150166561824	6.480092965989362
>50	0.01906385474532609	0.8688494354190963
>100	0.004127527428973121	0.4016285614907352
>500	4.672864675572992E-5	0.016932015246172605
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.14738380016379E-5	0.0
5	0.0	0.0	0.0	3.14738380016379E-5	0.0
6	0.0	0.0	0.0	3.14738380016379E-5	0.0
7	0.0	0.0	0.0	6.29476760032758E-5	0.0
8	0.0	0.0	0.0	6.29476760032758E-5	0.0
9	0.0	0.0	0.0	1.888430280098274E-4	0.0
10-11	0.0	0.0	0.0	2.203168660114653E-4	0.0
12-13	0.0	0.0	0.0	2.517907040131032E-4	0.0
14-15	0.0	0.0	0.0	2.675276230139221E-4	0.0
16-17	0.0	0.0	0.0	2.8326454201474106E-4	0.0
18-19	0.0	0.0	0.0	2.8326454201474106E-4	0.0
20-21	0.0	0.0	0.0	2.9900146101556006E-4	0.0
22-23	0.0	0.0	0.0	3.619491370188358E-4	0.0
24-25	0.0	0.0	0.0	4.091598940212927E-4	0.0
26-27	0.0	0.0	0.0	5.507921650286633E-4	0.0
28-29	0.0	0.0	0.0	0.0013691119530712486	0.0
30-31	0.0	0.0	0.0	0.004327652725225211	0.0
32-33	0.0	0.0	0.0	0.011786952331613393	0.0
34-35	0.0	0.0	0.0	0.02294442790319403	0.0
36-37	0.0	0.0	0.0	0.04250541822121198	0.0
38-39	0.0	0.0	0.0	0.078228224353071	0.0
40-41	0.0	0.0	0.0	0.11912847683619944	0.0
42-43	0.0	0.0	0.0	0.15985562321031888	0.0
44-45	0.0	0.0	0.0	0.2058703743687135	0.0
46-47	0.0	0.0	0.0	0.26521429592080176	0.0
48-49	0.0	0.0	0.0	0.332552572325306	0.0
50-51	0.0	6.29476760032758E-5	0.0	0.4053515596230945	0.0
52-53	0.0	6.29476760032758E-5	0.0	0.4794881850359526	0.0
54-55	0.0	7.868459500409475E-5	0.0	0.5603602117811611	0.0
56-57	0.0	9.44215140049137E-5	0.0	0.65372735221302	0.0
58-59	0.0	9.44215140049137E-5	0.0	0.7625796209416846	0.0
60-61	0.0	9.44215140049137E-5	0.0	0.8817080977778842	0.0
62-63	0.0	9.44215140049137E-5	0.0	0.9974688739479083	0.0
64-65	0.0	9.44215140049137E-5	0.0	1.121869218649382	0.0
66-67	0.0	9.44215140049137E-5	0.0	1.2645558632298075	0.0
68-69	0.0	1.258953520065516E-4	0.0	1.4234043236240739	0.0
70-71	0.0	1.258953520065516E-4	0.0	1.600554820816293	0.0
72-73	0.0	1.258953520065516E-4	0.0	1.783417819605809	0.0
74-75	0.0	1.258953520065516E-4	0.0	1.971096315609576	0.0
76-77	0.0	1.573691900081895E-4	0.0	2.170042445617929	0.0
78-79	0.0	1.573691900081895E-4	0.0	2.3963550777687064	0.0
80-81	0.0	1.573691900081895E-4	0.0	2.6514977455289843	0.0
82-83	0.0	1.573691900081895E-4	0.0	2.9151383495497036	0.0
84-85	0.0	1.573691900081895E-4	0.0	3.1739791932751737	0.0
86-87	0.0	1.573691900081895E-4	0.0	3.4436470372732075	0.0
88	0.0	1.573691900081895E-4	0.0	3.669251508068948	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	3215	0.0	37.399197	4
ATCAACG	3335	0.0	36.340313	3
GTATCAA	3710	0.0	35.28499	1
CAACGCA	3470	0.0	34.785645	5
GGTATCA	2090	0.0	34.02121	1
AACGCAG	3660	0.0	32.845303	6
TATCAAC	4365	0.0	28.321533	2
GTACATG	7830	0.0	26.46123	1
ACGCAGA	4435	0.0	26.153183	7
CGCAGAG	4485	0.0	25.233404	8
TACATGG	8115	0.0	24.15421	2
ACATGGG	8165	0.0	22.782629	3
TACACCG	360	0.0	20.874397	5
CTGTCGC	730	0.0	18.655048	9
GCAGAGT	5700	0.0	18.618935	9
CATGGGG	5150	0.0	18.05765	4
TCTGTCG	690	0.0	17.694794	8
TAGACTG	1465	0.0	17.632809	5
GTATAGA	1590	0.0	17.47326	1
GAGTACA	6080	0.0	16.88387	1
>>END_MODULE