FastQCFastQC Report
Wed 25 May 2016
SRR1033184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177242
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.19132946121195676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.15598434113611742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.054.0582851
GTATCAA83300.042.1967851
ATCAACG95250.035.8865053
TCAACGC95350.035.7995574
CAACGCA97250.035.196275
AACGCAG100400.034.1684466
TATCAAC103700.033.2812422
ACGCAGA115050.029.6950387
CGCAGAG115450.029.5916888
CGTACGA856.8018533E-427.6587982
GTACATG78750.027.1872061
GCAGAGT131500.025.872729
TACATGG79800.024.8062672
TCGTACG950.001296261924.7656761
GTGGTAT22650.023.6832031
ACATGGG82800.023.3952983
TGGTATC21650.023.0213182
GAGTACT93950.021.3329812-13
AGAGTAC123550.021.242710-11
CAGAGTA131100.021.16637610-11
Produced by FastQC (version 0.11.5)