FastQCFastQC Report
Wed 25 May 2016
SRR1033183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003923
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21600.034.3605044
ATCAACG22000.033.3178833
GTATCAA24000.032.381821
CAACGCA23100.032.12935
AACGCAG24350.030.6644426
GTACATG54700.027.3822041
TATCAAC28700.026.040092
TACATGG55600.025.1078172
ACGCAGA29750.025.098467
ACATGGG53600.024.1946743
CGCAGAG31400.023.3315038
GGTATCA16300.022.2500881
GTACCGG1503.3141354E-521.9151126
CATGGGG39200.020.011824
ACGGGTC1909.090274E-619.7784635
GTACACG2401.5460319E-719.6253491
CCTATAC8350.019.1323323
CTATACT10700.018.8773424
TATACCG1250.006433430618.789545
ATGTCGC2753.2376192E-818.7843846
Produced by FastQC (version 0.11.5)