##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033183_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003923 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.509247610811393 33.0 31.0 34.0 30.0 34.0 2 31.60528623105778 34.0 31.0 34.0 30.0 34.0 3 31.76737828748909 34.0 31.0 34.0 30.0 34.0 4 35.13521278013177 37.0 35.0 37.0 33.0 37.0 5 35.21029001613336 37.0 35.0 37.0 33.0 37.0 6 35.35147558064856 37.0 36.0 37.0 33.0 37.0 7 35.24786381512663 37.0 35.0 37.0 33.0 37.0 8 35.262546015989635 37.0 36.0 37.0 33.0 37.0 9 36.929735324161655 39.0 38.0 39.0 34.0 39.0 10-11 36.921898196687195 39.0 38.0 39.0 34.0 39.0 12-13 36.87957346664517 39.0 38.0 39.0 34.0 39.0 14-15 38.326275760096564 41.0 39.0 41.0 34.0 41.0 16-17 38.32866657052192 41.0 39.0 41.0 34.0 41.0 18-19 38.306587129345786 41.0 39.0 41.0 34.0 41.0 20-21 38.35043187787156 41.0 39.0 41.0 34.0 41.0 22-23 38.30326215129024 41.0 39.0 41.0 34.0 41.0 24-25 38.29602609481502 41.0 39.0 41.0 34.0 41.0 26-27 38.25813491835764 40.0 39.0 41.0 34.0 41.0 28-29 38.224926806069895 40.0 38.5 41.0 33.5 41.0 30-31 38.22239826580163 40.0 39.0 41.0 34.0 41.0 32-33 38.29832957653562 41.0 39.0 41.0 34.0 41.0 34-35 38.273139985917624 41.0 39.0 41.0 34.0 41.0 36-37 38.191039026948644 41.0 39.0 41.0 33.0 41.0 38-39 38.11312984580745 41.0 38.0 41.0 33.0 41.0 40-41 38.052167673109196 41.0 38.0 41.0 33.0 41.0 42-43 37.932810791632214 40.5 38.0 41.0 33.0 41.0 44-45 37.795491892652564 40.0 38.0 41.0 33.0 41.0 46-47 37.67459353478152 40.0 38.0 41.0 32.5 41.0 48-49 37.561963957696975 40.0 38.0 41.0 32.0 41.0 50-51 37.4379449709395 40.0 37.5 41.0 32.0 41.0 52-53 37.299551429870306 40.0 37.0 41.0 31.5 41.0 54-55 37.155245735489835 40.0 37.0 41.0 31.0 41.0 56-57 37.00419626901832 40.0 36.5 41.0 31.0 41.0 58-59 36.819298446097974 40.0 36.0 41.0 31.0 41.0 60-61 36.605502307224384 39.5 35.5 41.0 31.0 41.0 62-63 36.35031984761889 39.0 35.0 41.0 30.0 41.0 64-65 36.09001219108718 39.0 35.0 41.0 30.0 41.0 66-67 35.80624754543962 38.5 35.0 40.5 30.0 41.0 68-69 35.503711719462274 37.5 35.0 40.0 30.0 41.0 70-71 35.16187647928588 37.0 35.0 40.0 29.0 41.0 72-73 34.79531873230658 36.5 34.5 39.0 29.0 41.0 74-75 34.42474960365244 36.0 34.0 39.0 29.0 41.0 76-77 34.092837399440995 36.0 34.0 39.0 29.0 40.0 78-79 33.71700284891186 35.0 34.0 37.5 28.0 39.5 80-81 33.34667474748281 35.0 34.0 37.0 28.0 39.0 82-83 33.11863130469584 35.0 34.0 37.0 28.0 39.0 84-85 32.839798235760554 35.0 34.0 36.0 28.0 37.5 86-87 32.57282265835563 35.0 34.0 36.0 27.0 37.0 88-89 32.20474539191376 35.0 34.0 35.5 26.5 37.0 90-91 32.00852477864669 35.0 33.5 35.0 26.0 36.0 92-93 31.826044962805454 35.0 33.0 35.0 26.0 36.0 94-95 31.655543900638897 35.0 33.0 35.0 25.5 36.0 96-97 31.47891585654738 35.0 33.0 35.0 25.0 36.0 98-99 31.28307874104943 35.0 33.0 35.0 24.5 35.5 100 31.120963230623133 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21131503275918817 1101 2 0.12695169705134646 1101 3 0.22283745650516096 1101 4 0.15506007015216738 1101 5 0.25779598075694565 1101 6 0.19834176747975363 1101 7 0.25256553469552756 1101 8 0.14847421166012964 1101 9 0.2199241122184432 1101 10-11 0.22922093650429787 1101 12-13 0.2535233841862592 1101 14-15 0.327743825082635 1101 16-17 0.296752716337771 1101 18-19 0.25507551344821877 1101 20-21 0.26939450019489897 1101 22-23 0.2580404244253174 1101 24-25 0.3472255203908503 1101 26-27 0.24246199961108772 1101 28-29 0.24079529692280488 1101 30-31 0.35292604308789777 1101 32-33 0.34069604569240397 1101 34-35 0.2965725393044423 1101 36-37 0.27014606075071157 1101 38-39 0.2892440583847886 1101 40-41 0.221078390656821 1101 42-43 0.24567932322695185 1101 44-45 0.14105834686065322 1101 46-47 0.13038744221484677 1101 48-49 0.13695961642461896 1101 50-51 0.13963466856938567 1101 52-53 0.11628478233286899 1101 54-55 0.0983802619673213 1101 56-57 0.020104210517409626 1101 58-59 0.07997729002144638 1101 60-61 0.02780868039162243 1101 62-63 0.03341012862583881 1101 64-65 -0.03986062425372694 1101 66-67 -0.07159166285897811 1101 68-69 -0.050768540023319986 1101 70-71 0.010107321998134466 1101 72-73 0.050030572217742986 1101 74-75 -0.05360588509660147 1101 76-77 -0.10452622264701716 1101 78-79 -0.10547839024760464 1101 80-81 -0.11373159868624327 1101 82-83 -0.09145557164253404 1101 84-85 -0.07873862846532376 1101 86-87 0.02836439176112293 1101 88-89 0.0588153406176275 1101 90-91 0.04170115285629805 1101 92-93 -0.007061284846582083 1101 94-95 -0.04548570905924976 1101 96-97 -0.037937965816116304 1101 98-99 0.012005559833319523 1101 100 -0.0046009420023658265 1102 1 0.04409202815369895 1102 2 0.08877181656567856 1102 3 0.0706875941623224 1102 4 0.013654444605023741 1102 5 0.024815988623075214 1102 6 0.014898054669394867 1102 7 0.006154136428612844 1102 8 0.08015756212130754 1102 9 0.05888426142104919 1102 10-11 0.0726657277348366 1102 12-13 0.0806802245420073 1102 14-15 0.09569793513225022 1102 16-17 0.10075555829212135 1102 18-19 0.016741482313591405 1102 20-21 0.08340254394069291 1102 22-23 0.04595069986651623 1102 24-25 0.039780932157050586 1102 26-27 0.05824341018468715 1102 28-29 0.015368052222925144 1102 30-31 0.04783812012516364 1102 32-33 0.01622066539070488 1102 34-35 0.05464483151824595 1102 36-37 0.07194797399497332 1102 38-39 0.05168363513659102 1102 40-41 0.09330970544133521 1102 42-43 0.06376125778587749 1102 44-45 0.044157218960016564 1102 46-47 0.12186379943836556 1102 48-49 0.06917995669789434 1102 50-51 0.10554168337546344 1102 52-53 0.04543194228160985 1102 54-55 0.05271202207592296 1102 56-57 0.05352540337699452 1102 58-59 0.11028026265495328 1102 60-61 0.008540166454629627 1102 62-63 0.09342928644311854 1102 64-65 0.14475698063849052 1102 66-67 0.11444469893196185 1102 68-69 0.1281420018511028 1102 70-71 0.1657322347729746 1102 72-73 0.1599174649720254 1102 74-75 0.11418487601429916 1102 76-77 0.057259641167540565 1102 78-79 0.14013910045894562 1102 80-81 0.16062008047741472 1102 82-83 0.17283888781521028 1102 84-85 0.12629600785332684 1102 86-87 0.11325699379870002 1102 88-89 0.32792018843350945 1102 90-91 0.3305931078417643 1102 92-93 0.3425628540031802 1102 94-95 0.31434789705095056 1102 96-97 0.35026855799159407 1102 98-99 0.3714452253961902 1102 100 0.3612975088787671 1103 1 -0.06176428730409356 1103 2 -0.029470838657442044 1103 3 0.022166552639450998 1103 4 0.006705790522261168 1103 5 -0.11550651135191714 1103 6 -0.012684495921291727 1103 7 -0.05460069756992425 1103 8 -0.09287657846226693 1103 9 -0.04656992937810145 1103 10-11 -0.02354934893892846 1103 12-13 -0.025540453868295288 1103 14-15 0.013510457014035637 1103 16-17 -0.01198764209999581 1103 18-19 0.012675221772823875 1103 20-21 -0.026527784461116255 1103 22-23 -0.013973876752231718 1103 24-25 -0.061466204455300044 1103 26-27 -0.04855144622080587 1103 28-29 -0.0019818262393940245 1103 30-31 -0.08566726654470358 1103 32-33 -0.0734766407742029 1103 34-35 -0.04604638275817763 1103 36-37 -0.03999022684728715 1103 38-39 -0.07415137371615543 1103 40-41 -0.04143381505001997 1103 42-43 -0.15196336684775957 1103 44-45 -0.24750594662131675 1103 46-47 -0.11086084520147921 1103 48-49 -0.09256343375314202 1103 50-51 -0.22708368048733973 1103 52-53 -0.17592967982123753 1103 54-55 -0.14302540652694518 1103 56-57 -0.19729835198715762 1103 58-59 -0.28313794699003836 1103 60-61 -0.2866458412077506 1103 62-63 -0.16750785407044333 1103 64-65 -0.20420494755924778 1103 66-67 -0.18547002463234463 1103 68-69 -0.3168596986760619 1103 70-71 -0.24186937172504486 1103 72-73 -0.21934710704529436 1103 74-75 -0.2521676932821819 1103 76-77 -0.23073663767890196 1103 78-79 -0.33256168678509823 1103 80-81 -0.2393939238639362 1103 82-83 -0.18999258994288937 1103 84-85 -0.1517836968551336 1103 86-87 -0.14926913930256092 1103 88-89 -0.10009999782423051 1103 90-91 -0.07694923028304856 1103 92-93 -0.05749791483515665 1103 94-95 -0.06712235872834071 1103 96-97 -0.09110526789443085 1103 98-99 -0.00577421262959632 1103 100 0.07643120032311757 1104 1 0.04037953870467703 1104 2 -0.06433526496065056 1104 3 -0.031035004822843604 1104 4 0.01413615138967117 1104 5 -0.057162453421732096 1104 6 -0.02116786105860058 1104 7 -0.1186593416593098 1104 8 -0.09809529270691542 1104 9 -0.035316338165642946 1104 10-11 -0.06101372623378154 1104 12-13 -0.017583108500261346 1104 14-15 -0.1626960360138554 1104 16-17 -0.10138471394741089 1104 18-19 -0.11303192630764869 1104 20-21 -0.13417805584004583 1104 22-23 -0.08081046476756626 1104 24-25 -0.1723051541329852 1104 26-27 -0.03936479192842057 1104 28-29 0.031842860849984334 1104 30-31 -0.032544334687536036 1104 32-33 -0.03968909584184388 1104 34-35 -0.09536947900556214 1104 36-37 -0.06586625115080125 1104 38-39 -0.14432954994941127 1104 40-41 -0.1131239664935535 1104 42-43 -0.04017777453164939 1104 44-45 -0.13035331261530558 1104 46-47 -0.15790572836286287 1104 48-49 -0.2510415411045841 1104 50-51 -0.1929760859291889 1104 52-53 -0.21058041813014228 1104 54-55 -0.21249788177676976 1104 56-57 -0.24629850441471746 1104 58-59 -0.2979007484927081 1104 60-61 -0.2676085562704813 1104 62-63 -0.2497188832651389 1104 64-65 -0.2924913724385121 1104 66-67 -0.2747064687097591 1104 68-69 -0.31095216624662214 1104 70-71 -0.29749554025589475 1104 72-73 -0.19109591415498528 1104 74-75 -0.2946192492973765 1104 76-77 -0.3493489102193692 1104 78-79 -0.27163118410218345 1104 80-81 -0.2579360604351635 1104 82-83 -0.16486133824255234 1104 84-85 -0.16991509905355429 1104 86-87 -0.19185816953530832 1104 88-89 -0.028312408514224785 1104 90-91 -0.08874186513801874 1104 92-93 -0.13895910658708388 1104 94-95 -0.12050938579291426 1104 96-97 0.007147796574706433 1104 98-99 -0.013357483416182703 1104 100 -0.01258263199499865 1105 1 0.2515188519383784 1105 2 0.22762783375254259 1105 3 0.16263422542747463 1105 4 0.33226129508359037 1105 5 0.16711948999464 1105 6 0.24648807753287372 1105 7 0.24557852873829944 1105 8 0.14738130302409758 1105 9 0.15593722808224442 1105 10-11 0.18078969727240235 1105 12-13 0.15568783777094808 1105 14-15 0.1606829703791135 1105 16-17 0.1744390049911857 1105 18-19 0.2055489651659812 1105 20-21 0.1697567827248605 1105 22-23 0.28552378805387946 1105 24-25 0.24249106322353953 1105 26-27 0.24424214686450796 1105 28-29 0.27440685827829725 1105 30-31 0.3671935056519402 1105 32-33 0.3472666397769544 1105 34-35 0.3131317974323977 1105 36-37 0.3101747697058741 1105 38-39 0.29644710163005783 1105 40-41 0.22581866882884327 1105 42-43 0.281987089947485 1105 44-45 0.22801467552297794 1105 46-47 0.26715602847283293 1105 48-49 0.23617750143156968 1105 50-51 0.25371111816458836 1105 52-53 0.24446684655715245 1105 54-55 0.23257140335832105 1105 56-57 0.1972874923518546 1105 58-59 0.2566094967403245 1105 60-61 0.17908381047907795 1105 62-63 0.2770431196801013 1105 64-65 0.29795388253013755 1105 66-67 0.2655608696681355 1105 68-69 0.28063914094241227 1105 70-71 0.2398943632414472 1105 72-73 0.19399398030198256 1105 74-75 0.19710340732726905 1105 76-77 0.21081656551821482 1105 78-79 0.26638053260247574 1105 80-81 0.19570070933012573 1105 82-83 0.26501849578150427 1105 84-85 0.20032567803397683 1105 86-87 0.3122069629889168 1105 88-89 0.36894448890862463 1105 90-91 0.373108173708097 1105 92-93 0.41236646838362745 1105 94-95 0.40090037717923366 1105 96-97 0.4298659371375315 1105 98-99 0.48529362935412124 1105 100 0.5252354277550566 1106 1 -0.05886189002399078 1106 2 -0.033860332405428295 1106 3 -0.07950281215427424 1106 4 -0.06419728302660133 1106 5 -0.1642190531265726 1106 6 -0.03322233802180108 1106 7 -0.08135940163518285 1106 8 -0.037249767648454224 1106 9 -0.1453710577278926 1106 10-11 -0.056610553577392864 1106 12-13 -0.09086975274897924 1106 14-15 0.013230164458306604 1106 16-17 -0.11149682615395307 1106 18-19 -0.01307544812696193 1106 20-21 -0.06622887135470279 1106 22-23 -0.07313534981381054 1106 24-25 9.406638413622659E-4 1106 26-27 -0.021073048607199496 1106 28-29 -0.020632716389734185 1106 30-31 -0.012584442149929487 1106 32-33 -0.045250005049148 1106 34-35 -0.08400590390431262 1106 36-37 -0.05848646683931946 1106 38-39 -0.12888531291218186 1106 40-41 -0.11556427702032579 1106 42-43 -0.12052410713044992 1106 44-45 -0.10265653221910753 1106 46-47 -0.06940458612569245 1106 48-49 -0.09453017152065968 1106 50-51 -0.14572565408043658 1106 52-53 -0.2546676279940385 1106 54-55 -0.18153578563116923 1106 56-57 -0.16627423327884117 1106 58-59 -0.1434894330443086 1106 60-61 -0.20285538956675708 1106 62-63 -0.15869487943746208 1106 64-65 -0.08248247714021772 1106 66-67 -0.09713340259477832 1106 68-69 -0.030316231038533203 1106 70-71 -0.07589512783751218 1106 72-73 -0.028849435772130505 1106 74-75 0.01575354456755207 1106 76-77 0.03532972466682338 1106 78-79 0.02191511974413629 1106 80-81 0.016389042500961182 1106 82-83 -0.06350688306834229 1106 84-85 -0.008959730538876443 1106 86-87 -0.018443037644544802 1106 88-89 0.07400637786159336 1106 90-91 0.06526539935558873 1106 92-93 0.058259147193975735 1106 94-95 0.11914735902209372 1106 96-97 0.12459864218961769 1106 98-99 0.06182446586714363 1106 100 0.09797256041075286 1107 1 -0.0036439416777866995 1107 2 -0.09423195449871002 1107 3 -0.07070691112319594 1107 4 -0.17576918090462357 1107 5 -0.18375981997569824 1107 6 -0.12100243190459281 1107 7 -0.16878799883802742 1107 8 -0.17725144297634898 1107 9 -0.15335012901631018 1107 10-11 -0.15606011170966383 1107 12-13 -0.18604964108193656 1107 14-15 -0.1755152297323832 1107 16-17 -0.2237018583664323 1107 18-19 -0.11628246603256542 1107 20-21 -0.20192393375529605 1107 22-23 -0.14860398520553986 1107 24-25 -0.16471500687509177 1107 26-27 -0.13122091777267997 1107 28-29 -0.12395120803618198 1107 30-31 -0.1893239104051503 1107 32-33 -0.17248133239210972 1107 34-35 -0.13329147320972368 1107 36-37 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2214 9 0.13921930015439443 2214 10-11 0.05984958370090965 2214 12-13 0.11870199199089626 2214 14-15 0.08627119496347291 2214 16-17 0.08616148754633457 2214 18-19 0.11849754088873254 2214 20-21 0.09548822927865785 2214 22-23 0.11486962728947958 2214 24-25 0.09223787237994685 2214 26-27 0.09705054265840118 2214 28-29 0.2215461941549961 2214 30-31 0.17234758163409225 2214 32-33 0.19835880279182305 2214 34-35 0.2044651666828443 2214 36-37 0.16947404270182886 2214 38-39 0.23977525476316686 2214 40-41 0.23163967202555824 2214 42-43 0.25502281328689236 2214 44-45 0.3041869847979015 2214 46-47 0.31927250390072004 2214 48-49 0.3074861672487259 2214 50-51 0.2788793903361366 2214 52-53 0.2853858146723809 2214 54-55 0.22348313348069837 2214 56-57 0.24567285440181763 2214 58-59 0.3622342357335384 2214 60-61 0.31841968718058666 2214 62-63 0.35116651639523155 2214 64-65 0.31594280001712605 2214 66-67 0.29664623819707003 2214 68-69 0.23017495194140736 2214 70-71 0.37694885833266056 2214 72-73 0.3242842014908689 2214 74-75 0.3502934174882384 2214 76-77 0.3625885442366368 2214 78-79 0.2934487406887669 2214 80-81 0.21314372638350676 2214 82-83 0.2343696888497817 2214 84-85 0.20432954319161212 2214 86-87 0.2306681210618251 2214 88-89 0.19309683263421107 2214 90-91 0.12961281748326314 2214 92-93 0.1715412816369586 2214 94-95 0.06361492115939171 2214 96-97 0.0899537197528737 2214 98-99 -0.02044358378150335 2214 100 -0.0064405391619111185 2215 1 -0.1405988729150316 2215 2 -0.15660518117919864 2215 3 -0.1621554950294275 2215 4 -0.24103596621956314 2215 5 -0.09849920567702242 2215 6 -0.18056065043241176 2215 7 -0.0469143833609067 2215 8 -0.08985583788522433 2215 9 -0.16599317754355525 2215 10-11 -0.19459025324368895 2215 12-13 -0.17147419419185184 2215 14-15 -0.2262088286027648 2215 16-17 -0.11771173425290016 2215 18-19 -0.21091564093378423 2215 20-21 -0.11977811919621928 2215 22-23 -0.08316898506991777 2215 24-25 -0.0983823273128337 2215 26-27 -0.16477968031438905 2215 28-29 -0.05370550891051806 2215 30-31 -0.09928659798445238 2215 32-33 -0.09072017031213875 2215 34-35 -0.1353268160288934 2215 36-37 -0.30351632806078754 2215 38-39 -0.17667882686948388 2215 40-41 -0.08476616801031156 2215 42-43 -0.1685321451621249 2215 44-45 -0.12563311996210302 2215 46-47 -0.0865287717287444 2215 48-49 0.04734141860593155 2215 50-51 0.025576123026411324 2215 52-53 -0.18789138190049925 2215 54-55 -0.06599834985597397 2215 56-57 -0.10386196303147699 2215 58-59 -0.04504440152781797 2215 60-61 -0.09327761796868117 2215 62-63 0.019020439170510883 2215 64-65 0.032758663810042776 2215 66-67 0.022676306605916352 2215 68-69 -0.0015776193535899097 2215 70-71 0.01656176871420456 2215 72-73 0.04039880301170484 2215 74-75 0.08689012489490722 2215 76-77 -0.015282397267597503 2215 78-79 0.005566266520979468 2215 80-81 -0.018215875662079384 2215 82-83 -0.010296305177568854 2215 84-85 -0.0741899466520124 2215 86-87 -0.10728811683306105 2215 88-89 0.010017289456484946 2215 90-91 -0.008532868561147211 2215 92-93 -0.061728007846014066 2215 94-95 -0.1311800550358555 2215 96-97 -0.1883976102601963 2215 98-99 -0.2800146808037631 2215 100 -0.18482711118202033 2216 1 0.05175339057100814 2216 2 -0.013856468791018983 2216 3 -0.05081547515050744 2216 4 0.008090942608482976 2216 5 0.058325136963262025 2216 6 0.04185734902543459 2216 7 0.03231779338075569 2216 8 0.04448499522221283 2216 9 -0.012878060529011748 2216 10-11 -0.028497929217309093 2216 12-13 -0.06943152228437555 2216 14-15 -0.06706057252613107 2216 16-17 -0.067833153788456 2216 18-19 -0.0644373722069389 2216 20-21 -0.17035810925676032 2216 22-23 -0.006772942782035329 2216 24-25 -0.07302823406263315 2216 26-27 -0.13341729665123836 2216 28-29 -0.12120742903341153 2216 30-31 -0.12250427215089843 2216 32-33 -0.07166746858629125 2216 34-35 -0.12872724071489472 2216 36-37 -0.07284462389524293 2216 38-39 -0.02168933107116544 2216 40-41 -0.006943587365149995 2216 42-43 -0.011261091579406468 2216 44-45 0.01876238017343468 2216 46-47 0.054998968399566195 2216 48-49 0.10396301885893422 2216 50-51 0.03069663897472452 2216 52-53 0.10943456460714174 2216 54-55 0.12056022518694931 2216 56-57 0.09284389673179305 2216 58-59 0.008712827170114679 2216 60-61 0.08622507020336201 2216 62-63 0.09668037411223196 2216 64-65 0.11847859694459117 2216 66-67 0.1049626082233388 2216 68-69 0.09653105960185826 2216 70-71 0.06885518608115149 2216 72-73 0.17938972195993586 2216 74-75 0.12574098782071985 2216 76-77 0.08295843132190583 2216 78-79 0.16899809086361728 2216 80-81 0.13201736279459197 2216 82-83 0.06151967928073532 2216 84-85 0.09804790009958708 2216 86-87 0.08979612008751303 2216 88-89 0.11894021346479633 2216 90-91 0.07501619622605915 2216 92-93 0.12028947656710542 2216 94-95 0.09433329546563485 2216 96-97 0.14357864798504139 2216 98-99 0.17565137594151636 2216 100 0.20936056388800495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24473.0 3 3932.0 4 2579.0 5 2087.0 6 1750.0 7 1935.0 8 2200.0 9 2425.0 10 2629.0 11 2958.0 12 3182.0 13 3558.0 14 3894.0 15 4031.0 16 4064.0 17 4252.0 18 4173.0 19 4158.0 20 4343.0 21 4466.0 22 5134.0 23 6669.0 24 9609.0 25 13357.0 26 14715.0 27 13322.0 28 13916.0 29 17735.0 30 21723.0 31 26248.0 32 34424.0 33 46540.0 34 65161.0 35 99314.0 36 183410.0 37 393821.0 38 655850.0 39 295103.0 40 783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01601209669344 19.855550659910676 13.302857943962668 23.825579299433215 2 15.140451010016228 20.002428115782152 41.046348033925675 23.810772840275945 3 18.332361248157365 25.428150255072484 29.806228802217955 26.4332596945522 4 11.980094664169846 16.693733303845427 39.24119893304365 32.084973098941084 5 12.690094302650149 38.13368719917285 35.89486669871829 13.281351799458719 6 30.975979797677333 38.41161234298034 17.88404625373678 12.728361605605546 7 27.209088427416393 32.485867952503746 22.627445441837647 17.677598178242214 8 24.812076982930336 37.31968199447698 20.22161964411291 17.646621378479775 9 24.61063388130329 17.277848567836422 21.108397630837246 37.00311992002304 10-11 23.72114021151741 27.194247333322352 29.118008627802126 19.96660382735811 12-13 24.277745433292846 24.339359389540203 29.196854968133678 22.18604020903327 14-15 21.492835064487164 26.47290321260426 26.10446216941001 25.92979955349857 16-17 21.4393344992673 29.931586229201855 26.591954057410266 22.03712521412058 18-19 21.310516041863924 29.02401700191511 28.474988507835384 21.190478448385583 20-21 22.4174118339976 28.376163297087796 27.921875350954473 21.284549517960134 22-23 21.9466101284637 28.157602237171968 28.014135548944125 21.8816520854202 24-25 21.79777894353821 28.30641574754887 28.02303114460426 21.872774164308666 26-27 21.646918718678897 28.159811014645815 28.098469634894013 22.094800631781272 28-29 21.728424453957544 28.247543239435142 28.134568938281145 21.88946336832617 30-31 21.900607824758005 28.7742982663668 27.683627662664744 21.641466246210452 32-33 21.49286920236641 28.37963869959807 28.316947077739357 21.810545020296164 34-35 21.599622881829653 28.096276931308196 28.360698856467508 21.943401330394646 36-37 21.89387738805821 28.509672010061287 27.7420996945681 21.854350907312398 38-39 21.831791485165116 28.653047229664175 27.882156418067773 21.633004867102937 40-41 21.625929145859555 28.35113853119345 28.161117160963446 21.86181516198355 42-43 21.57795120368811 28.263607383701704 28.15398723635207 22.00445417625812 44-45 21.725402731464698 28.32084866000723 28.13359727527276 21.82015133325531 46-47 21.68054653770123 28.484419892590907 28.055322519227783 21.779711050480078 48-49 21.676983158006628 28.389826385016097 28.05823646261498 21.874953994362297 50-51 21.677036177924744 28.314030754704454 28.08069803687111 21.92823503049969 52-53 21.754454694490676 28.206693831923086 28.049241809874815 21.989609663711427 54-55 21.80764263443028 28.615864150461306 27.860897745555587 21.715595469552824 56-57 21.61083303807133 28.339088863049998 28.001816485101326 22.04826161377734 58-59 21.537840432144126 28.036969491760562 28.334793425816112 22.0903966502792 60-61 21.719490094500042 28.455035440323595 28.007000483815876 21.81847398136049 62-63 21.66534380873795 28.473225663260838 27.939557910112367 21.921872617888848 64-65 21.53478866108898 28.214734575973544 28.124359835189612 22.126116927747866 66-67 21.46332006159047 28.238471187043746 28.01777450413111 22.28043424723468 68-69 21.87888003481302 28.271755143104667 28.002098961597888 21.847265860484427 70-71 21.787868686223472 28.158210704076907 28.083779802302743 21.97014080739688 72-73 21.59319981106705 28.292310114525005 28.225315268761612 21.88917480564633 74-75 21.585731060339533 28.34300708077392 28.037698920642335 22.033562938244213 76-77 21.615014079130077 28.3308027911236 27.971519859869925 22.082663269876402 78-79 21.778694343285387 28.32307187512086 28.09458756423548 21.80364621735827 80-81 21.727771879417272 28.06651188148828 28.220789703832992 21.984926535261454 82-83 21.535031260690225 28.220804246972552 28.3568656619963 21.887298830340924 84-85 21.583311833194184 28.149778702436244 28.082982718878487 22.183926745491085 86-87 21.656903920202648 28.25102451449092 28.10904966175552 21.983021903550917 88-89 21.53337624117285 28.042247821891635 28.1716239947868 22.25275194214872 90-91 21.49125097281149 28.053171993071075 28.243893254336854 22.211683779780582 92-93 21.68508803925787 28.214761515418452 28.050484158807805 22.049666286515876 94-95 21.413584692164832 28.02513253642885 28.227617527012434 22.333665244393885 96-97 21.442892612348864 27.96048701491024 28.316250463089855 22.280369909651043 98-99 21.41106653494611 28.06533922059981 28.22732940581177 22.296264838642315 100 21.62333923504038 27.893923885548716 28.117158004275932 22.365578875134972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 312.0 1 282.5 2 242.5 3 212.0 4 178.0 5 151.0 6 140.0 7 141.0 8 147.5 9 147.0 10 146.5 11 155.0 12 157.0 13 185.0 14 219.5 15 263.5 16 342.0 17 458.5 18 618.0 19 860.0 20 1334.0 21 2025.0 22 3031.5 23 4572.5 24 6670.5 25 9215.0 26 12786.0 27 17236.0 28 22370.0 29 28286.5 30 34187.0 31 39937.5 32 45838.0 33 52107.0 34 57578.5 35 61925.5 36 65686.0 37 69239.0 38 72065.5 39 72855.0 40 73332.5 41 73870.5 42 74285.5 43 73770.5 44 71602.5 45 69746.5 46 67848.0 47 65284.0 48 63494.5 49 62307.5 50 60288.0 51 57565.5 52 54262.5 53 51930.0 54 50290.0 55 47985.5 56 44276.0 57 40710.0 58 36815.0 59 31978.5 60 27609.5 61 23505.0 62 19992.5 63 16360.5 64 13132.5 65 10766.5 66 8534.0 67 6516.5 68 5222.0 69 4266.0 70 3396.5 71 2481.0 72 1830.5 73 1363.5 74 977.0 75 677.0 76 421.0 77 314.0 78 238.0 79 140.5 80 94.5 81 72.5 82 51.5 83 35.5 84 21.5 85 22.5 86 21.5 87 14.5 88 10.5 89 6.0 90 6.5 91 5.5 92 3.0 93 4.0 94 3.5 95 0.5 96 2.5 97 3.0 98 1.0 99 0.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3334958478943552 2 0.11831791940109476 3 0.06761736853162521 4 0.04201758251190291 5 0.045111513765748484 6 0.009631108580519313 7 0.007185904847641352 8 0.015469656269227909 9 0.017266132481138247 10-11 0.019960846799003756 12-13 0.015893824263706738 14-15 0.017266132481138247 16-17 0.018912902342056056 18-19 0.019162412927043605 20-21 0.022855169584859298 22-23 0.01606848167319802 24-25 0.023054778052849337 26-27 0.019037657634549832 28-29 0.01846378328907847 30-31 0.01921231504404111 32-33 0.023229435462340617 34-35 0.015319949918235381 36-37 0.010130129750494406 38-39 0.013323865238335005 40-41 0.010030325516499386 42-43 0.009256842703037992 44-45 0.009955472341003122 46-47 0.009082185293546707 48-49 0.004615945822269618 50-51 0.006587079443671239 52-53 0.0068864921456562945 54-55 0.0029442249028530537 56-57 0.0026448122008679974 58-59 0.0027196653763642614 60-61 0.003068980195346827 62-63 0.0027196653763642614 64-65 0.002220644206389168 66-67 0.0020709378553966395 68-69 0.0037426587748132043 70-71 0.0025450079668729786 72-73 0.0018713293874066022 74-75 0.0022954973818854318 76-77 0.003518099248324412 78-79 0.003268588663336865 80-81 0.003518099248324412 82-83 0.002420252674379205 84-85 0.002994127019850563 86-87 0.0023453994988829408 88-89 0.6016698246389707 90-91 0.6124486819104327 92-93 0.6072838128011905 94-95 0.5950078920198032 96-97 0.5922882266434389 98-99 0.5901923377295435 100 0.5910406737185012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2003923.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.23216303924129 #Duplication Level Percentage of deduplicated Percentage of total 1 82.06031324566224 59.27393925411886 2 11.752732595449654 16.978505939822497 3 3.125489323906493 6.772825632654655 4 1.2051678684177929 3.482075278448356 5 0.5715621567994459 2.0642585448498987 6 0.33096720709800814 1.4343886358247393 7 0.2125633625848853 1.074773802368056 8 0.1417852607173329 0.8193164854956578 9 0.10449071224902035 0.6792831146931915 >10 0.4704910023691958 5.845772321471947 >50 0.01896588276343949 0.9155841955602919 >100 0.005471381982446042 0.659276794691871 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.990211699750939E-5 0.0 0.0 0.0 6 0.0 4.990211699750939E-5 0.0 0.0 0.0 7 0.0 4.990211699750939E-5 0.0 0.0 0.0 8 0.0 4.990211699750939E-5 0.0 0.0 0.0 9 0.0 4.990211699750939E-5 0.0 1.4970635099252817E-4 0.0 10-11 0.0 9.980423399501878E-5 0.0 2.4951058498754694E-4 0.0 12-13 0.0 9.980423399501878E-5 0.0 3.2436376048381105E-4 0.0 14-15 0.0 1.2475529249377347E-4 0.0 3.493148189825657E-4 0.0 16-17 0.0 1.4970635099252817E-4 0.0 3.742658774813204E-4 0.0 18-19 0.0 1.4970635099252817E-4 0.0 3.992169359800751E-4 0.0 20-21 0.0 1.4970635099252817E-4 0.0 4.241679944788298E-4 0.0 22-23 0.0 1.4970635099252817E-4 0.0 4.7407011147633917E-4 0.0 24-25 0.0 1.7465740949128286E-4 0.0 4.990211699750939E-4 0.0 26-27 0.0 1.9960846799003755E-4 0.0 5.738743454713579E-4 0.0 28-29 0.0 2.9941270198505633E-4 0.0 0.0011726997494414706 0.0 30-31 0.0 2.9941270198505633E-4 0.0 0.003967218301301996 0.0 32-33 0.0 2.9941270198505633E-4 0.0 0.010454493510978217 4.990211699750939E-5 34-35 0.0 2.9941270198505633E-4 0.0 0.01823922376258968 4.990211699750939E-5 36-37 0.0 3.992169359800751E-4 0.0 0.0329852993353537 4.990211699750939E-5 38-39 0.0 4.4911905297758444E-4 0.0 0.05888449805706107 4.990211699750939E-5 40-41 0.0 4.990211699750939E-4 0.0 0.09431500112529273 4.990211699750939E-5 42-43 0.0 4.990211699750939E-4 0.0 0.12984530842751943 4.990211699750939E-5 44-45 0.0 5.489232869726032E-4 0.0 0.16859430227608546 4.990211699750939E-5 46-47 0.0 5.988254039701127E-4 0.0 0.2150531732007667 4.990211699750939E-5 48-49 0.0 6.48727520967622E-4 0.0 0.27049442518499967 4.990211699750939E-5 50-51 0.0 6.986296379651314E-4 0.0 0.3319738333259312 4.990211699750939E-5 52-53 0.0 6.986296379651314E-4 0.0 0.3954493261467631 4.990211699750939E-5 54-55 0.0 6.986296379651314E-4 0.0 0.4596483996640589 4.990211699750939E-5 56-57 0.0 6.986296379651314E-4 0.0 0.5366473661912159 4.990211699750939E-5 58-59 0.0 7.984338719601502E-4 0.0 0.6267955405472166 4.990211699750939E-5 60-61 0.0 7.984338719601502E-4 0.0 0.723680500697881 4.990211699750939E-5 62-63 0.0 8.233849304589049E-4 0.0 0.8244328749158526 4.990211699750939E-5 64-65 0.0 8.483359889576596E-4 0.0 0.9284288867386621 4.990211699750939E-5 66-67 0.0 8.982381059551689E-4 0.0 1.042056007141991 4.990211699750939E-5 68-69 0.0 8.982381059551689E-4 0.0 1.1777149121997201 4.990211699750939E-5 70-71 0.0 8.982381059551689E-4 0.0 1.32278036631148 4.990211699750939E-5 72-73 0.0 9.73091281451433E-4 0.0 1.4774020758282629 4.990211699750939E-5 74-75 0.0 0.0010229933984489425 0.0 1.6373633118637794 4.990211699750939E-5 76-77 0.0 0.001047944456947697 0.0 1.806232075783351 4.990211699750939E-5 78-79 0.0 0.001047944456947697 0.0 1.9980558135217772 4.990211699750939E-5 80-81 0.0 0.001047944456947697 0.0 2.208493040900274 4.990211699750939E-5 82-83 0.0 0.0010728955154464517 0.0 2.432678301511585 4.990211699750939E-5 84-85 0.0 0.0010978465739452064 0.0 2.6542437009805266 4.990211699750939E-5 86-87 0.0 0.0010978465739452064 0.0 2.8829201521216135 4.990211699750939E-5 88 0.0 0.0010978465739452064 0.0 3.0660858725609716 4.990211699750939E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 2160 0.0 34.360504 4 ATCAACG 2200 0.0 33.317883 3 GTATCAA 2400 0.0 32.38182 1 CAACGCA 2310 0.0 32.1293 5 AACGCAG 2435 0.0 30.664442 6 GTACATG 5470 0.0 27.382204 1 TATCAAC 2870 0.0 26.04009 2 TACATGG 5560 0.0 25.107817 2 ACGCAGA 2975 0.0 25.09846 7 ACATGGG 5360 0.0 24.194674 3 CGCAGAG 3140 0.0 23.331503 8 GGTATCA 1630 0.0 22.250088 1 GTACCGG 150 3.3141354E-5 21.915112 6 CATGGGG 3920 0.0 20.01182 4 ACGGGTC 190 9.090274E-6 19.778463 5 GTACACG 240 1.5460319E-7 19.625349 1 CCTATAC 835 0.0 19.132332 3 CTATACT 1070 0.0 18.877342 4 TATACCG 125 0.0064334306 18.78954 5 ATGTCGC 275 3.2376192E-8 18.784384 6 >>END_MODULE