FastQCFastQC Report
Wed 25 May 2016
SRR1033183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003923
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.1712640655354522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.1419715228579142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.047.669671
GTATCAA49050.041.1562541
TCAACGC56750.034.6279454
CAACGCA57500.034.2580345
ATCAACG57400.034.2358133
AACGCAG60250.032.682156
TATCAAC61100.032.624282
ACGCAGA68750.028.436387
CGCAGAG71100.027.3643058
TATACCG1555.074071E-827.2977645
GCAGAGT80900.023.87529
GTACATG52550.023.460981
GTGGTAT14100.023.027541
TACATGG52550.022.3657552
ACATGGG50950.022.145393
ATAACGC1703.4457953E-622.123683
GAGTACT53350.021.0526312-13
AGAGTAC73100.020.60409410-11
TATAACG1857.1675386E-620.3298682
CAGAGTA81350.019.2077710-11
Produced by FastQC (version 0.11.5)