##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033183_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2003923
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75074391580914	34.0	31.0	34.0	31.0	34.0
2	32.83469324919171	34.0	31.0	34.0	31.0	34.0
3	32.929433416353824	34.0	31.0	34.0	31.0	34.0
4	36.36107824502239	37.0	37.0	37.0	35.0	37.0
5	36.29615309570278	37.0	37.0	37.0	35.0	37.0
6	36.38378121315041	37.0	37.0	37.0	35.0	37.0
7	36.35919394108456	37.0	37.0	37.0	35.0	37.0
8	36.354514619573706	37.0	37.0	37.0	35.0	37.0
9	38.166219959549345	39.0	39.0	39.0	37.0	39.0
10-11	38.15059036699514	39.0	39.0	39.0	37.0	39.0
12-13	38.11502961940155	39.0	39.0	39.0	37.0	39.0
14-15	39.651492098249285	41.0	40.0	41.0	37.0	41.0
16-17	39.670238327520565	41.0	40.0	41.0	37.0	41.0
18-19	39.68283037821313	41.0	40.0	41.0	37.0	41.0
20-21	39.67465167074783	41.0	40.0	41.0	37.0	41.0
22-23	39.60956658514324	41.0	40.0	41.0	37.0	41.0
24-25	39.56671588678806	41.0	40.0	41.0	37.0	41.0
26-27	39.47472707284661	41.0	39.0	41.0	36.5	41.0
28-29	39.38530821793053	41.0	39.0	41.0	36.0	41.0
30-31	39.2980259221537	41.0	39.0	41.0	36.0	41.0
32-33	39.34232078777478	41.0	39.0	41.0	36.0	41.0
34-35	39.29316495693697	41.0	39.0	41.0	35.5	41.0
36-37	39.217389340808005	41.0	39.0	41.0	35.0	41.0
38-39	39.13456679722724	41.0	39.0	41.0	35.0	41.0
40-41	39.027524011651145	41.0	39.0	41.0	35.0	41.0
42-43	38.91997172546051	41.0	39.0	41.0	35.0	41.0
44-45	38.79174873485658	41.0	39.0	41.0	35.0	41.0
46-47	38.60452697034766	41.0	39.0	41.0	35.0	41.0
48-49	38.44285808386849	41.0	39.0	41.0	35.0	41.0
50-51	38.324029166789344	41.0	38.0	41.0	34.5	41.0
52-53	38.19366337928154	40.0	38.0	41.0	34.0	41.0
54-55	38.062818281939975	40.0	38.0	41.0	34.0	41.0
56-57	37.913372919019345	40.0	37.5	41.0	34.0	41.0
58-59	37.733011448044664	40.0	37.0	41.0	34.0	41.0
60-61	37.54722911010054	40.0	37.0	41.0	33.5	41.0
62-63	37.31987631261281	39.5	36.0	41.0	33.0	41.0
64-65	37.072437164501835	39.0	35.5	41.0	33.0	41.0
66-67	36.79754012504472	39.0	35.0	41.0	33.0	41.0
68-69	36.47989593412521	38.5	35.0	41.0	32.0	41.0
70-71	36.14698394099973	37.0	35.0	40.0	32.0	41.0
72-73	35.796301804011435	37.0	35.0	39.5	32.0	41.0
74-75	35.455708378016524	36.5	35.0	39.0	31.0	41.0
76-77	35.111694161901426	36.0	35.0	39.0	31.0	40.5
78-79	34.761813951933284	35.5	35.0	38.0	31.0	39.5
80-81	34.41002149284179	35.0	35.0	37.0	31.0	39.0
82-83	34.080482882825336	35.0	34.5	37.0	31.0	39.0
84-85	33.80162461332097	35.0	34.0	36.0	31.0	38.0
86-87	33.56368034101111	35.0	34.0	36.0	30.0	37.0
88-89	33.341992930866105	35.0	34.0	36.0	30.0	37.0
90-91	33.13784960799392	35.0	34.0	35.0	30.0	36.5
92-93	32.96977578479812	35.0	34.0	35.0	30.0	36.0
94-95	32.8198865425468	35.0	34.0	35.0	30.0	36.0
96-97	32.69290237199733	35.0	34.0	35.0	29.5	36.0
98-99	32.58859946215498	35.0	34.0	35.0	30.0	36.0
100	32.489798260711616	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1083380333264401
1101	2	0.13401799950872118
1101	3	0.13587956919676714
1101	4	0.03167489813355928
1101	5	0.029703925092036343
1101	6	0.08894522548952466
1101	7	0.1098461028189206
1101	8	0.10672613601796854
1101	9	0.1183542374964972
1101	10-11	0.12479037767058543
1101	12-13	0.18524294557231968
1101	14-15	0.21176858669824838
1101	16-17	0.17279119577543156
1101	18-19	0.2121236619087199
1101	20-21	0.21699675959959563
1101	22-23	0.23526675715716294
1101	24-25	0.17550454477268573
1101	26-27	0.17591642735916935
1101	28-29	0.15964918295598096
1101	30-31	0.16315291818188626
1101	32-33	0.16672361339656305
1101	34-35	0.19791141017518754
1101	36-37	0.28331613671609546
1101	38-39	0.25371169592081344
1101	40-41	0.3501215795328676
1101	42-43	0.24038999458963417
1101	44-45	0.19348242803513216
1101	46-47	0.3059255261259253
1101	48-49	0.20259903546751445
1101	50-51	0.2758183760179804
1101	52-53	0.27957943769651195
1101	54-55	0.26665136336525563
1101	56-57	0.23891757797803592
1101	58-59	0.2457998290517054
1101	60-61	0.24559902442520354
1101	62-63	0.2727303567862194
1101	64-65	0.3601547056835557
1101	66-67	0.32467831806150116
1101	68-69	0.3552925232526931
1101	70-71	0.3494234915668386
1101	72-73	0.45486543160822634
1101	74-75	0.4166834239833719
1101	76-77	0.3671317394829856
1101	78-79	0.3987848549030417
1101	80-81	0.40211808903694646
1101	82-83	0.37249026365363846
1101	84-85	0.32500842501203664
1101	86-87	0.23579966300390254
1101	88-89	0.24148243784159007
1101	90-91	0.2889041377733079
1101	92-93	0.24634542177459195
1101	94-95	0.2456361348171825
1101	96-97	0.22907034344736132
1101	98-99	0.252645126157212
1101	100	0.2132227767976005
1102	1	0.1584509502627114
1102	2	0.13455974705690465
1102	3	0.12493931367462352
1102	4	0.05791286106128268
1102	5	0.06821811583284898
1102	6	0.10633887422903854
1102	7	0.13895773833827718
1102	8	0.1493827289714389
1102	9	0.17813931469869715
1102	10-11	0.1887311787464725
1102	12-13	0.18565750297599237
1102	14-15	0.20679167757735684
1102	16-17	0.1750714200868373
1102	18-19	0.18083234890043798
1102	20-21	0.15591028298862142
1102	22-23	0.22584961154506544
1102	24-25	0.2423317057364116
1102	26-27	0.22377671485330097
1102	28-29	0.1826947049747787
1102	30-31	0.22687549776295413
1102	32-33	0.164804659844755
1102	34-35	0.22170199366394172
1102	36-37	0.18466796812730024
1102	38-39	0.1815580908255967
1102	40-41	0.25486631113312086
1102	42-43	0.23716583544019443
1102	44-45	0.19058401521588308
1102	46-47	0.09984165041068138
1102	48-49	0.17865905906588608
1102	50-51	0.13220135474139028
1102	52-53	0.0791207919385073
1102	54-55	0.10056989823628726
1102	56-57	0.07813432774648987
1102	58-59	0.011361749400862209
1102	60-61	0.04915463918813856
1102	62-63	0.13536500071725044
1102	64-65	0.11616182325673208
1102	66-67	0.04787640481723088
1102	68-69	0.0713460763299949
1102	70-71	0.11351780892687202
1102	72-73	0.11685175470291398
1102	74-75	0.0829152335724288
1102	76-77	0.04998422428995042
1102	78-79	0.11725375199637966
1102	80-81	0.001358119993945195
1102	82-83	-0.004869256259077304
1102	84-85	0.0916430562557693
1102	86-87	0.11111938286840228
1102	88-89	0.15911651084429934
1102	90-91	0.11457497856042664
1102	92-93	0.0902441010270536
1102	94-95	0.0991400488480636
1102	96-97	0.10823970616447554
1102	98-99	0.15841467410884746
1102	100	0.1803404768445418
1103	1	0.09297915400587442
1103	2	0.08848396356401622
1103	3	0.0974322864961934
1103	4	0.023066058552501545
1103	5	-0.04743273593119568
1103	6	0.03936911360324302
1103	7	0.11107159363341168
1103	8	0.11159151369470521
1103	9	0.13346496565048938
1103	10-11	0.08484447386796035
1103	12-13	0.11054521816770091
1103	14-15	0.11528155944073148
1103	16-17	0.14603716946913892
1103	18-19	0.12668781409464458
1103	20-21	0.09769748702187542
1103	22-23	0.09868268979932537
1103	24-25	0.08779730007066178
1103	26-27	0.1427303404772431
1103	28-29	0.09512905962472473
1103	30-31	0.17766074118841857
1103	32-33	0.11042080084846617
1103	34-35	0.133435631091551
1103	36-37	0.1299987706760959
1103	38-39	0.14568772101095817
1103	40-41	0.15027088655150322
1103	42-43	0.08234659147456114
1103	44-45	0.07854428289886783
1103	46-47	0.15495348507943163
1103	48-49	0.04083683417616868
1103	50-51	0.03283530126481082
1103	52-53	0.03774546558733505
1103	54-55	0.02557464970924883
1103	56-57	0.04448514650029978
1103	58-59	0.081530001559031
1103	60-61	-2.5992932810225966E-4
1103	62-63	0.012720730993635243
1103	64-65	-0.051389117534910156
1103	66-67	-0.05439554948860348
1103	68-69	-0.008069792589367353
1103	70-71	0.005419048311082975
1103	72-73	0.0233895314136916
1103	74-75	-0.01865900444135349
1103	76-77	-0.05527440588539889
1103	78-79	-0.07950324267196862
1103	80-81	-0.04465014256744837
1103	82-83	-0.056033117055925175
1103	84-85	-0.03238514248582902
1103	86-87	0.002914839459947416
1103	88-89	-0.045444903194059805
1103	90-91	-0.05256183385142066
1103	92-93	-0.04351261355446212
1103	94-95	-0.08463717209042443
1103	96-97	-0.08038576773243022
1103	98-99	-0.059702192119679864
1103	100	-0.04209247737126276
1104	1	0.092306253888367
1104	2	0.13905325153439918
1104	3	0.1278242182549576
1104	4	0.06975760885649152
1104	5	0.02379000317264257
1104	6	0.04904729851909195
1104	7	0.08849495055121537
1104	8	0.11957997164881817
1104	9	0.11862257381134356
1104	10-11	0.11894748595218374
1104	12-13	0.08917328336038821
1104	14-15	0.10140606959838294
1104	16-17	0.14019885651414654
1104	18-19	0.13573988836352413
1104	20-21	0.08465697216404777
1104	22-23	0.0896679988703113
1104	24-25	0.08323894443595492
1104	26-27	0.0726963915809904
1104	28-29	0.12090089294230211
1104	30-31	0.04092309602047095
1104	32-33	0.020648689688265165
1104	34-35	0.07392424822632648
1104	36-37	0.042218588573405214
1104	38-39	0.022192701918925195
1104	40-41	0.07374043673585362
1104	42-43	0.03874314622596842
1104	44-45	0.05298469047857424
1104	46-47	0.06279704038458078
1104	48-49	0.12116422540753291
1104	50-51	0.19856119407543815
1104	52-53	0.11547073518151763
1104	54-55	0.07903692581921717
1104	56-57	0.10000881232507908
1104	58-59	0.09839374676190715
1104	60-61	0.12228796475983472
1104	62-63	0.1457704493885572
1104	64-65	0.18571242429873536
1104	66-67	0.09506135499719903
1104	68-69	0.13112647042726877
1104	70-71	0.07181378936753902
1104	72-73	0.17682691745479673
1104	74-75	0.16339761950057863
1104	76-77	0.08817246346488616
1104	78-79	0.09467562623840564
1104	80-81	0.14529918974199063
1104	82-83	0.15530358576244652
1104	84-85	0.2031048156255295
1104	86-87	0.21007883800127303
1104	88-89	0.16924472343561092
1104	90-91	0.21563517007889743
1104	92-93	0.29273340451688057
1104	94-95	0.22123331465512308
1104	96-97	0.14752767271181
1104	98-99	0.1308783156763127
1104	100	0.0444550192473514
1105	1	-0.009847445487203288
1105	2	0.04073597903198589
1105	3	0.011164603393666539
1105	4	0.06539963902383761
1105	5	0.0486836409910012
1105	6	0.022555646648214633
1105	7	0.0029777373390373896
1105	8	-0.018772204995691766
1105	9	-0.01821109367966045
1105	10-11	-0.04175468800774951
1105	12-13	0.013568254520642142
1105	14-15	0.005649990628569412
1105	16-17	0.03710549642373451
1105	18-19	0.06395298765034596
1105	20-21	0.0897363944605658
1105	22-23	0.11504560698664079
1105	24-25	0.06271933804600849
1105	26-27	-0.009931570005178969
1105	28-29	0.007268602067725283
1105	30-31	0.026350066009605655
1105	32-33	0.006186484688150529
1105	34-35	0.013968261587741893
1105	36-37	-0.004807813477704315
1105	38-39	-0.061182754861391686
1105	40-41	-0.015216137757136039
1105	42-43	-0.09035319168431499
1105	44-45	0.026232840756371445
1105	46-47	0.11653787781782654
1105	48-49	0.15509153567305134
1105	50-51	0.16156141526656143
1105	52-53	0.16728733607977375
1105	54-55	0.1879455587433796
1105	56-57	0.08172830710988421
1105	58-59	0.0540391911656144
1105	60-61	0.04663882389458962
1105	62-63	0.023943016091216407
1105	64-65	-0.03309784970750229
1105	66-67	0.026313480290497182
1105	68-69	-0.002548340080316791
1105	70-71	0.06389180879839529
1105	72-73	-0.016492284733615747
1105	74-75	0.025994511077001903
1105	76-77	0.009966832820097693
1105	78-79	-0.032663573095632614
1105	80-81	0.052448596082456334
1105	82-83	0.07242833296439954
1105	84-85	0.12474269914775249
1105	86-87	0.0781237081340933
1105	88-89	0.10884136399224076
1105	90-91	0.09000268738297024
1105	92-93	0.10151798455892447
1105	94-95	0.09785180724223608
1105	96-97	0.10799572596838658
1105	98-99	0.1280391991742178
1105	100	0.10121440415248628
1106	1	-0.029860896853243446
1106	2	0.014240423436035599
1106	3	-0.03571819951407207
1106	4	0.005372945608669966
1106	5	-0.0822500391474108
1106	6	-0.036013429862073565
1106	7	-0.09148456330725452
1106	8	-0.029262067171522688
1106	9	-0.005803668217140512
1106	10-11	-0.007843254401699085
1106	12-13	0.016996810525867545
1106	14-15	0.028074387286324054
1106	16-17	0.07009902026850767
1106	18-19	-0.018718711435660396
1106	20-21	0.015144989343646387
1106	22-23	0.04526232160341692
1106	24-25	0.06582505024957186
1106	26-27	0.02375225899611877
1106	28-29	-0.03809015335592392
1106	30-31	0.028662522183871886
1106	32-33	0.06340194175091796
1106	34-35	0.009206797493028773
1106	36-37	0.026049025319068164
1106	38-39	0.10010523822454331
1106	40-41	0.054818272842233284
1106	42-43	0.004947863878861369
1106	44-45	-0.05322865291812917
1106	46-47	-0.01724862663924398
1106	48-49	0.06315491903863801
1106	50-51	0.03526008373555811
1106	52-53	0.018024820167553912
1106	54-55	-0.014628543576236552
1106	56-57	0.01951276466784435
1106	58-59	-0.021738379865809065
1106	60-61	-0.03280217875547464
1106	62-63	-0.08595701304790992
1106	64-65	-0.0822424320624151
1106	66-67	-0.05457092774749128
1106	68-69	-0.011140909660291243
1106	70-71	-0.01413652500039575
1106	72-73	-0.012781756808813327
1106	74-75	-0.03561968589792741
1106	76-77	-0.1299322764262527
1106	78-79	-0.04101058088276943
1106	80-81	-0.04761298715764184
1106	82-83	-0.05116768759052093
1106	84-85	-0.005830129643676685
1106	86-87	-0.029712290960340226
1106	88-89	8.521779651431416E-4
1106	90-91	-0.046919274313140136
1106	92-93	-0.012458271191093218
1106	94-95	-0.062143708969074396
1106	96-97	-0.006044261274297469
1106	98-99	0.029722713098472298
1106	100	0.040388340450732585
1107	1	-0.04865678714504185
1107	2	-0.014918658613147784
1107	3	-0.041189755990345134
1107	4	0.0034557690946215303
1107	5	-0.13519555555112817
1107	6	-0.11272680166351279
1107	7	-0.11488986056519224
1107	8	-0.05421972755463145
1107	9	-0.0631081515449452
1107	10-11	-0.06997960890966937
1107	12-13	-0.039047955094687836
1107	14-15	-0.028194453440463008
1107	16-17	-0.0946757608917892
1107	18-19	-0.07261819820539728
1107	20-21	-0.0815564940732898
1107	22-23	-0.05373677737087945
1107	24-25	-0.06856959256730022
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2215	86-87	-0.1748467657736228
2215	88-89	-0.25247592468860347
2215	90-91	-0.1665310122229826
2215	92-93	-0.13512664610502156
2215	94-95	-0.07790692471144212
2215	96-97	-0.13178913653252522
2215	98-99	-0.15242544093511157
2215	100	-0.21796286988879388
2216	1	-0.07040953009964568
2216	2	-0.07232385744872971
2216	3	-0.11633251420546742
2216	4	-0.049915376616766594
2216	5	-0.03418667587106228
2216	6	-0.029947555236361723
2216	7	-0.0072577691413115986
2216	8	-0.07421522084678145
2216	9	-0.07424759751299348
2216	10-11	-0.09798137861690037
2216	12-13	-0.1204319540834291
2216	14-15	-0.16654552964595837
2216	16-17	-0.16044768117068742
2216	18-19	-0.13016949864709204
2216	20-21	-0.14523847438666593
2216	22-23	-0.20331780010044298
2216	24-25	-0.20627965370097456
2216	26-27	-0.2278801180926422
2216	28-29	-0.18793077926146395
2216	30-31	-0.12378604344927169
2216	32-33	-0.20855787665920644
2216	34-35	-0.1814239375596074
2216	36-37	-0.22755862463746013
2216	38-39	-0.2555791044780591
2216	40-41	-0.21254384441846952
2216	42-43	-0.1492055216132684
2216	44-45	-0.1017218432977316
2216	46-47	-0.14193229320932232
2216	48-49	-0.13642974241215455
2216	50-51	-0.11045613378593089
2216	52-53	0.036415624765254506
2216	54-55	-0.036665634274584136
2216	56-57	-0.002166941697460345
2216	58-59	-0.037835163406811034
2216	60-61	-0.0073892549652683215
2216	62-63	-0.011857718157536112
2216	64-65	-0.011234028701068155
2216	66-67	0.036996733799881554
2216	68-69	0.03369633558263274
2216	70-71	0.049178456164483464
2216	72-73	0.07330923131058142
2216	74-75	0.08810773610535705
2216	76-77	0.09891443467197547
2216	78-79	0.06994759549122165
2216	80-81	0.03855044373195682
2216	82-83	-0.012739212752308049
2216	84-85	0.01624076768077032
2216	86-87	0.029483111338514334
2216	88-89	-0.013819897761706557
2216	90-91	-0.10125135197178281
2216	92-93	0.009685068903564797
2216	94-95	-0.06281984149104858
2216	96-97	-0.03816055606807822
2216	98-99	-0.025121293469673844
2216	100	-0.09365976154868605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11.0
3	7.0
4	5.0
5	15.0
6	47.0
7	87.0
8	205.0
9	384.0
10	726.0
11	1177.0
12	1697.0
13	2706.0
14	3338.0
15	4350.0
16	5492.0
17	7840.0
18	9111.0
19	5092.0
20	3574.0
21	3284.0
22	3499.0
23	3664.0
24	4314.0
25	5160.0
26	6611.0
27	8461.0
28	10894.0
29	14160.0
30	18558.0
31	24005.0
32	30762.0
33	40425.0
34	56304.0
35	87090.0
36	161142.0
37	375888.0
38	723676.0
39	378532.0
40	1630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81613616021481	20.035092941853403	13.533658456669755	23.615112441262028
2	15.464394430859823	20.13977743400369	40.5899995009731	23.80582863416338
3	18.4251091484054	25.32881752442584	30.205402103773448	26.04067122339531
4	12.194842012235013	16.77965348933416	39.03217741617559	31.99332708225524
5	12.474084916110758	37.859859613004744	36.04863674053765	13.617418730346845
6	30.873487963591362	38.58392550600823	17.46626611840792	13.076320411992496
7	27.037184531330784	32.64281051818405	22.55584766079595	17.764157289689216
8	25.01972385111374	36.73097565320754	20.080202842530063	18.169097653148654
9	24.942886998595753	16.402085510314272	21.33925372344832	37.31577376764165
10-11	23.88517685242873	26.806556061592385	29.01088942578196	20.29737766019692
12-13	24.74638496588941	24.04383801173997	29.31953972283366	21.890237299536956
14-15	21.72226350240354	25.95671186638595	26.703173428493436	25.617851202717073
16-17	21.04327361879673	29.634771395906927	27.170929222330397	22.151025762965943
18-19	20.85065771088666	28.209833725897244	29.889017525649724	21.05049103756637
20-21	21.965639398320196	27.54574402309869	29.55338104308399	20.935235535497124
22-23	21.38874943984734	27.31900706515935	29.717533352163112	21.574710142830195
24-25	21.278198452833994	27.48612470944478	29.71460466026123	21.521072177460002
26-27	21.20112670658396	27.40227961216502	29.711658828176247	21.68493485307478
28-29	21.26338804250237	27.47764563091049	29.773669031070288	21.48529729551685
30-31	21.49504298080091	27.99415441139765	29.271672607102566	21.239130000698875
32-33	21.09750722635319	27.580585505565203	29.879866089723077	21.442041178358526
34-35	21.202777117779423	27.351759932753378	29.880022282431817	21.56544066703538
36-37	21.509429630047432	27.756075988098345	29.28151824456092	21.452976137293298
38-39	21.50008972097152	27.902223857193164	29.297703168086187	21.299983253749126
40-41	21.359598406544738	27.663086192691683	29.503381762120323	21.47393363864326
42-43	21.298714781781435	27.687645403756378	29.31182501585996	21.70181479860223
44-45	21.542391970845	27.751156289859964	29.171571628329147	21.534880110965894
46-47	21.584999321227222	27.95066519732324	28.897328629837254	21.567006851612287
48-49	21.574140755968056	27.999000852918265	28.74209777966761	21.684760611446073
50-51	21.642033871237395	27.97536138219801	28.608793945397785	21.773810801166803
52-53	21.76956265596839	27.889424728823048	28.52708450869755	21.813928106511014
54-55	21.74559845097413	28.25188138773555	28.38253054136059	21.619989619929736
56-57	21.461974617141212	27.969301432426825	28.586855847080052	21.98186810335191
58-59	21.42419781426219	27.701631817954986	28.859149658166576	22.01502070961625
60-61	21.612968260019922	28.058972767212865	28.542453046746658	21.78560592602056
62-63	21.469905299240327	28.066870902658874	28.506740137063343	21.956483661037453
64-65	21.445829248495464	27.756520124849448	28.74157217581026	22.056078450844836
66-67	21.41717941424515	27.83642977978053	28.586812781013123	22.159578024961203
68-69	21.7844725091977	27.91614840579421	28.539259118792256	21.76011996621584
70-71	21.727523804034554	27.812913175686887	28.529136347883107	21.930426672395452
72-73	21.584890746145913	27.991911816442943	28.621006621551555	21.802190815859586
74-75	21.59525627398436	28.00125754150635	28.456667215593516	21.946818968915768
76-77	21.628159505926657	28.023297418600507	28.267494646723684	22.081048428749153
78-79	21.719377870152165	28.085442426041922	28.43440801727612	21.760771686529793
80-81	21.723286405898072	27.787532321195147	28.55757707621028	21.931604196696508
82-83	21.534079340809377	27.95254981256662	28.590302368567084	21.92306847805692
84-85	21.601609449101023	27.87547678532353	28.362024247030377	22.16088951854507
86-87	21.632550201604854	27.970677472154577	28.323261407641027	22.073510918599546
88-89	21.420808084941388	27.786821150313664	28.45099337649201	22.34137738825294
90-91	21.4461886624857	27.780490512981416	28.465172680081192	22.308148144451696
92-93	21.563658275207747	27.934937603624892	28.33036207737575	22.171042043791616
94-95	21.38031251699791	27.689686679578006	28.423896526962363	22.50610427646172
96-97	21.332680946323787	27.693928359522797	28.516864170928724	22.456526523224696
98-99	21.325470090417646	27.827416522491134	28.357302151829188	22.48981123526203
100	21.489298740520468	27.585790471989192	28.297294856139683	22.627615931350658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	37.5
2	104.5
3	188.0
4	138.5
5	101.0
6	227.0
7	520.5
8	955.0
9	1139.0
10	878.0
11	639.0
12	646.0
13	724.5
14	845.5
15	980.5
16	1131.5
17	1349.0
18	1571.0
19	1834.5
20	2303.0
21	2953.0
22	3894.0
23	5343.0
24	7258.0
25	9717.5
26	13084.5
27	17322.5
28	22295.0
29	27914.0
30	33460.5
31	39180.5
32	45178.5
33	51371.5
34	57015.0
35	61152.0
36	64888.5
37	68576.5
38	71481.5
39	72509.5
40	72947.0
41	73577.0
42	73832.5
43	73040.0
44	70962.0
45	69755.0
46	68023.0
47	64830.0
48	63253.0
49	62203.5
50	59829.5
51	57378.5
52	54280.0
53	51925.0
54	50403.0
55	47785.0
56	44437.0
57	41091.5
58	36756.0
59	31897.0
60	27612.5
61	23729.0
62	20221.0
63	16247.5
64	12863.5
65	10369.0
66	8100.5
67	6283.5
68	4994.5
69	4003.0
70	3022.5
71	2153.5
72	1627.0
73	1265.0
74	922.0
75	576.5
76	311.0
77	193.0
78	130.0
79	76.5
80	42.0
81	20.0
82	14.0
83	7.5
84	0.5
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0903727338824895
2	0.0011477486909427159
3	0.0
4	9.980423399501878E-5
5	0.037226979280142
6	1.4970635099252817E-4
7	0.003942267242803241
8	1.9960846799003755E-4
9	4.990211699750939E-5
10-11	3.24363760483811E-4
12-13	0.0
14-15	9.980423399501878E-5
16-17	0.0
18-19	1.4970635099252817E-4
20-21	0.0
22-23	2.495105849875469E-4
24-25	4.990211699750939E-5
26-27	0.0014721124514265268
28-29	0.008782772591561652
30-31	0.0350811882492491
32-33	0.024551841562774616
34-35	0.02749606646562767
36-37	0.025150666966744728
38-39	0.024427086270280843
40-41	0.03011592760799691
42-43	0.022455952648879222
44-45	0.021183448665442733
46-47	0.015319949918235381
48-49	0.010679053037467008
50-51	0.007884534485606482
52-53	0.005564086045222297
54-55	0.004141875710793279
56-57	0.0025450079668729786
58-59	0.0011477486909427159
60-61	7.485317549626408E-4
62-63	0.0015968677439203004
64-65	0.003468197131326902
66-67	6.48727520967622E-4
68-69	0.0012725039834364893
70-71	4.241679944788298E-4
72-73	5.988254039701127E-4
74-75	6.48727520967622E-4
76-77	4.990211699750938E-4
78-79	2.4951058498754694E-5
80-81	6.736785794663767E-4
82-83	5.489232869726032E-4
84-85	3.742658774813204E-4
86-87	1.4970635099252817E-4
88-89	0.0
90-91	2.4951058498754694E-5
92-93	2.4951058498754694E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2003923.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.30010936049862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80491536461462	56.80594386998242
2	12.667498768782051	17.810530975387195
3	3.2712300329368635	6.899034871764272
4	1.278108289905217	3.594046102195865
5	0.582547472980897	2.0476575529119585
6	0.3546665449098355	1.495985813620298
7	0.23540466058181575	1.1584281368010894
8	0.15121888114509305	0.8504563105497828
9	0.10316579726150728	0.6527310146772292
>10	0.5215822610403734	6.298891022502745
>50	0.021904525755025193	1.0229701432473952
>100	0.007473005787609304	0.9314764592054372
>500	7.109857478561791E-5	0.04443433211113225
>1k	2.1329572435685372E-4	0.38741339504317895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3432	0.1712640655354522	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2845	0.1419715228579142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.990211699750939E-5	0.0
4	0.0	0.0	0.0	4.990211699750939E-5	0.0
5	0.0	4.990211699750939E-5	0.0	4.990211699750939E-5	0.0
6	0.0	4.990211699750939E-5	0.0	4.990211699750939E-5	0.0
7	0.0	4.990211699750939E-5	0.0	4.990211699750939E-5	0.0
8	0.0	4.990211699750939E-5	0.0	1.4970635099252817E-4	0.0
9	0.0	4.990211699750939E-5	0.0	2.9941270198505633E-4	0.0
10-11	0.0	4.990211699750939E-5	0.0	2.9941270198505633E-4	0.0
12-13	0.0	7.485317549626408E-5	0.0	3.493148189825657E-4	0.0
14-15	0.0	9.980423399501878E-5	0.0	3.493148189825657E-4	0.0
16-17	0.0	9.980423399501878E-5	0.0	3.493148189825657E-4	0.0
18-19	0.0	9.980423399501878E-5	0.0	3.992169359800751E-4	0.0
20-21	0.0	9.980423399501878E-5	0.0	5.738743454713579E-4	0.0
22-23	0.0	9.980423399501878E-5	0.0	6.237764624688674E-4	0.0
24-25	0.0	1.2475529249377347E-4	0.0	6.48727520967622E-4	0.0
26-27	0.0	1.4970635099252817E-4	0.0	7.485317549626408E-4	0.0
28-29	0.0	1.9960846799003755E-4	0.0	0.0013972592759302627	0.0
30-31	0.0	1.9960846799003755E-4	0.0	0.004241679944788298	0.0
32-33	0.0	1.9960846799003755E-4	0.0	0.010753906212963273	0.0
34-35	0.0	1.9960846799003755E-4	0.0	0.018488734347577227	0.0
36-37	0.0	1.9960846799003755E-4	0.0	0.03338451627133378	0.0
38-39	0.0	2.4951058498754694E-4	0.0	0.05930866605153991	0.0
40-41	0.0	2.4951058498754694E-4	0.0	0.09471421806127281	0.0
42-43	0.0	2.4951058498754694E-4	0.0	0.13049403594848705	0.0
44-45	0.0	2.4951058498754694E-4	0.0	0.16944263826504313	0.0
46-47	0.0	2.4951058498754694E-4	0.0	0.2161011176577144	0.0
48-49	0.0	2.4951058498754694E-4	0.0	0.27186673340243117	0.0
50-51	0.0	3.493148189825657E-4	0.0	0.333720407420844	0.0
52-53	0.0	3.493148189825657E-4	0.0	0.3976699703531523	0.0
54-55	0.0	3.493148189825657E-4	0.0	0.46224330974792943	0.0
56-57	0.0	3.992169359800751E-4	0.0	0.5393420805090814	0.0
58-59	0.0	4.4911905297758444E-4	0.0	0.6300641292105535	0.0
60-61	0.0	4.4911905297758444E-4	0.0	0.7274481105311931	0.0
62-63	0.0	4.4911905297758444E-4	0.0	0.8290488207381221	0.0
64-65	0.0	4.4911905297758444E-4	0.0	0.9337684132573956	0.0
66-67	0.0	4.990211699750939E-4	0.0	1.0477198974212083	0.0
68-69	0.0	5.489232869726032E-4	0.0	1.1845764532868777	0.0
70-71	0.0	5.489232869726032E-4	0.0	1.3304902433875951	0.0
72-73	0.0	5.489232869726032E-4	0.0	1.4862097994783232	0.0
74-75	0.0	5.489232869726032E-4	0.0	1.6476930500822635	0.0
76-77	0.0	5.988254039701127E-4	0.0	1.818133730687257	0.0
78-79	0.0	5.988254039701127E-4	0.0	2.012203063690571	0.0
80-81	2.4951058498754694E-5	6.487275209676221E-4	0.0	2.2250355926849483	0.0
82-83	4.990211699750939E-5	6.986296379651314E-4	0.0	2.4525143930180953	0.0
84-85	4.990211699750939E-5	6.986296379651314E-4	0.0	2.6774481853843684	0.0
86-87	4.990211699750939E-5	6.986296379651314E-4	0.0	2.909542931539785	0.0
88	4.990211699750939E-5	6.986296379651314E-4	0.0	3.0965261639294526	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2685	0.0	47.66967	1
GTATCAA	4905	0.0	41.156254	1
TCAACGC	5675	0.0	34.627945	4
CAACGCA	5750	0.0	34.258034	5
ATCAACG	5740	0.0	34.235813	3
AACGCAG	6025	0.0	32.68215	6
TATCAAC	6110	0.0	32.62428	2
ACGCAGA	6875	0.0	28.43638	7
CGCAGAG	7110	0.0	27.364305	8
TATACCG	155	5.074071E-8	27.297764	5
GCAGAGT	8090	0.0	23.8752	9
GTACATG	5255	0.0	23.46098	1
GTGGTAT	1410	0.0	23.02754	1
TACATGG	5255	0.0	22.365755	2
ACATGGG	5095	0.0	22.14539	3
ATAACGC	170	3.4457953E-6	22.12368	3
GAGTACT	5335	0.0	21.05263	12-13
AGAGTAC	7310	0.0	20.604094	10-11
TATAACG	185	7.1675386E-6	20.329868	2
CAGAGTA	8135	0.0	19.20777	10-11
>>END_MODULE