FastQCFastQC Report
Wed 25 May 2016
SRR1033182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059802
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.033.9221651
TCAACGC21100.028.502474
GTATCAA23150.027.8925131
ATCAACG21400.027.8901213
CAACGCA21950.027.1833555
GTACATG49550.027.014241
TACATGG48600.025.7477152
ACATGGG47950.025.3846763
AACGCAG24000.025.2406986
GTACGAC1151.3785303E-424.5196293
TATCAAC27900.021.9195942
ACGCAGA29350.020.4797557
CGCAGAG29050.019.8834828
CTTAGAC5450.019.8331553
GAGTACA35450.019.278351
GTGTAGG6900.019.1261271
CATGGGG32200.018.5311684
GTACTAG4101.8189894E-1218.3930681
ACACCGT1851.6614204E-417.7684366
GTGTAAG10850.016.9415421
Produced by FastQC (version 0.11.5)