##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033182_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2059802 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.571035468457648 33.0 31.0 34.0 30.0 34.0 2 31.760397844064624 34.0 31.0 34.0 30.0 34.0 3 31.97202061168986 34.0 31.0 34.0 30.0 34.0 4 35.32278296651814 37.0 35.0 37.0 33.0 37.0 5 35.454554369788944 37.0 35.0 37.0 35.0 37.0 6 35.510735983361506 37.0 37.0 37.0 35.0 37.0 7 35.4318837441657 37.0 37.0 37.0 35.0 37.0 8 35.465795256048885 37.0 37.0 37.0 35.0 37.0 9 37.147203954554854 39.0 38.0 39.0 35.0 39.0 10-11 37.143364507850755 39.0 38.0 39.0 35.0 39.0 12-13 37.108286621723835 39.0 38.0 39.0 35.0 39.0 14-15 38.58883305288566 41.0 39.0 41.0 35.0 41.0 16-17 38.61296425578769 41.0 39.0 41.0 35.0 41.0 18-19 38.601015534502835 41.0 39.0 41.0 35.0 41.0 20-21 38.6352695550349 41.0 39.0 41.0 35.0 41.0 22-23 38.604262448526605 41.0 39.0 41.0 35.0 41.0 24-25 38.599669774085086 41.0 39.0 41.0 34.0 41.0 26-27 38.55951979850491 41.0 39.0 41.0 34.0 41.0 28-29 38.536194498306145 41.0 39.0 41.0 34.0 41.0 30-31 38.536329948218324 41.0 39.0 41.0 34.0 41.0 32-33 38.599701087774456 41.0 39.0 41.0 35.0 41.0 34-35 38.57962294434125 41.0 39.0 41.0 34.0 41.0 36-37 38.50573283257323 41.0 39.0 41.0 34.0 41.0 38-39 38.43239398738325 41.0 39.0 41.0 34.0 41.0 40-41 38.375694605598014 41.0 39.0 41.0 34.0 41.0 42-43 38.27686835919181 41.0 39.0 41.0 33.5 41.0 44-45 38.168113003094476 40.5 38.0 41.0 33.0 41.0 46-47 38.054481450158804 40.0 38.0 41.0 33.0 41.0 48-49 37.95247358726712 40.0 38.0 41.0 33.0 41.0 50-51 37.84445009763074 40.0 38.0 41.0 33.0 41.0 52-53 37.709070095086815 40.0 38.0 41.0 33.0 41.0 54-55 37.57259338518945 40.0 37.5 41.0 32.0 41.0 56-57 37.436112305940085 40.0 37.0 41.0 32.0 41.0 58-59 37.253639184737175 40.0 37.0 41.0 32.0 41.0 60-61 37.053437660513 40.0 36.0 41.0 31.0 41.0 62-63 36.80845173468129 39.5 36.0 41.0 31.0 41.0 64-65 36.55471351129866 39.0 35.0 41.0 31.0 41.0 66-67 36.28210332837816 39.0 35.0 41.0 31.0 41.0 68-69 35.989147986068566 38.0 35.0 40.5 30.5 41.0 70-71 35.648334160273656 37.5 35.0 40.0 30.0 41.0 72-73 35.279996815227875 37.0 35.0 39.5 30.0 41.0 74-75 34.9078918750443 36.5 35.0 39.0 30.0 41.0 76-77 34.56597260319195 36.0 34.0 39.0 29.5 40.5 78-79 34.17789840965297 35.5 34.0 38.0 29.0 39.5 80-81 33.79869837003751 35.0 34.0 37.0 29.0 39.0 82-83 33.55327235336212 35.0 34.0 37.0 29.0 39.0 84-85 33.258948918391184 35.0 34.0 36.5 29.0 38.0 86-87 32.9810088542491 35.0 34.0 36.0 29.0 37.0 88-89 32.601235701295565 35.0 34.0 36.0 28.0 37.0 90-91 32.39500398582 35.0 34.0 35.0 27.5 36.5 92-93 32.21090376647852 35.0 34.0 35.0 27.0 36.0 94-95 32.03320731798493 35.0 34.0 35.0 27.0 36.0 96-97 31.85361117233598 35.0 34.0 35.0 27.0 36.0 98-99 31.664600044081908 35.0 33.5 35.0 26.0 36.0 100 31.506082137991903 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.030600617618475212 1101 2 -0.10883979166749214 1101 3 0.012001135498046267 1101 4 -0.105969661724572 1101 5 -0.08042623521720316 1101 6 -0.10109611767398974 1101 7 -0.04456768227582586 1101 8 -0.15140702123539285 1101 9 2.9533565906803005E-4 1101 10-11 -0.04044148130348191 1101 12-13 -0.03912121559435633 1101 14-15 -0.013579676402898144 1101 16-17 -0.07203399021236123 1101 18-19 -0.07369721802450613 1101 20-21 -0.016125162041262797 1101 22-23 -0.10993678985870048 1101 24-25 -0.06916382936690013 1101 26-27 -0.06970791254391884 1101 28-29 -0.06514313355396695 1101 30-31 -0.028452585564352262 1101 32-33 -1.1904963358944087E-4 1101 34-35 0.07295672760460548 1101 36-37 0.001507809107920366 1101 38-39 0.002879785671844104 1101 40-41 -0.025486314116847097 1101 42-43 0.009074516533864596 1101 44-45 -0.023667908995925302 1101 46-47 -0.0023084103131836287 1101 48-49 0.004857097958620216 1101 50-51 -0.032832637319096136 1101 52-53 -0.11055203905493016 1101 54-55 -0.09688369088945592 1101 56-57 -0.003620710525019888 1101 58-59 -0.03157408444267418 1101 60-61 -0.049100206680407155 1101 62-63 -0.027622974637381503 1101 64-65 -0.005163730665650235 1101 66-67 0.049823135343253 1101 68-69 0.027187259492798432 1101 70-71 0.011644181525753083 1101 72-73 0.05830743168463215 1101 74-75 -0.03829862598490763 1101 76-77 -0.12697947481584748 1101 78-79 -0.14374659430956882 1101 80-81 -0.11475409732808828 1101 82-83 -0.06902729482061432 1101 84-85 -0.1086292862379139 1101 86-87 -0.014542255978014396 1101 88-89 0.23711799773038678 1101 90-91 0.24014358104481914 1101 92-93 0.32344426609545707 1101 94-95 0.3079712585555967 1101 96-97 0.2967664172814004 1101 98-99 0.40777414934945355 1101 100 0.45270557224300845 1102 1 0.18015290561795183 1102 2 0.2630781905144417 1102 3 0.2583533484291678 1102 4 0.2626228997529765 1102 5 0.1874016016068012 1102 6 0.2882312763032928 1102 7 0.24630772149618707 1102 8 0.3874690598316093 1102 9 0.39575013273107373 1102 10-11 0.36603441936861003 1102 12-13 0.4298119471127322 1102 14-15 0.39063070509652675 1102 16-17 0.3863693519779048 1102 18-19 0.35896647245168367 1102 20-21 0.30680778575491985 1102 22-23 0.2915888333829315 1102 24-25 0.362304012146069 1102 26-27 0.36900218240763394 1102 28-29 0.386680190140936 1102 30-31 0.4304131407042675 1102 32-33 0.42043066642351334 1102 34-35 0.4325979240722546 1102 36-37 0.41823715812323314 1102 38-39 0.3535028644985587 1102 40-41 0.4118899492988106 1102 42-43 0.4042220072861866 1102 44-45 0.3652011711853831 1102 46-47 0.3978927430420356 1102 48-49 0.3717340354130343 1102 50-51 0.4409091569916299 1102 52-53 0.3939997577305192 1102 54-55 0.4456686692230818 1102 56-57 0.40631777936764735 1102 58-59 0.37945442707134447 1102 60-61 0.40208652485028296 1102 62-63 0.4103451078363065 1102 64-65 0.37471043248429936 1102 66-67 0.3031294257204493 1102 68-69 0.3666695684174641 1102 70-71 0.35048664206029656 1102 72-73 0.4087754327526696 1102 74-75 0.32098926997215926 1102 76-77 0.3093812531460074 1102 78-79 0.38021312291977694 1102 80-81 0.33785397680966156 1102 82-83 0.3766319932381563 1102 84-85 0.42864513871258936 1102 86-87 0.46440787166604025 1102 88-89 0.5550904923901783 1102 90-91 0.5632682391410953 1102 92-93 0.5609839106458381 1102 94-95 0.5676837663705356 1102 96-97 0.5276979583879999 1102 98-99 0.535303015792099 1102 100 0.5366885226441767 1103 1 -0.07701028880206096 1103 2 -0.09255288435365472 1103 3 -0.055891814152339236 1103 4 -0.030720768624995287 1103 5 -0.023060403032239662 1103 6 0.07031076233417366 1103 7 0.024355211104463592 1103 8 0.012642320366197168 1103 9 0.0228524191837991 1103 10-11 0.03983730927223661 1103 12-13 0.0047564607441259454 1103 14-15 -0.10454946631510609 1103 16-17 -0.05000523632066489 1103 18-19 -0.0247419948580756 1103 20-21 0.03406814679352266 1103 22-23 0.05240056436219476 1103 24-25 0.05033596009872099 1103 26-27 0.09313584064599922 1103 28-29 6.994983809036626E-4 1103 30-31 0.03721101240430613 1103 32-33 0.003055713036744123 1103 34-35 0.01194362662466375 1103 36-37 0.01642105937587246 1103 38-39 0.019822619209328707 1103 40-41 -0.0012302022401513568 1103 42-43 -0.03824806838165529 1103 44-45 -0.02758153567382493 1103 46-47 -0.09449395649038195 1103 48-49 -0.0912360938062946 1103 50-51 -0.01586229076675494 1103 52-53 -0.021198738450131316 1103 54-55 -0.03763421403547795 1103 56-57 -0.05283670922353423 1103 58-59 -0.08343811113588373 1103 60-61 -0.11582644500531813 1103 62-63 -0.07230929436892097 1103 64-65 -0.056059817597208905 1103 66-67 -0.08501795107792276 1103 68-69 -0.08924642377418479 1103 70-71 -0.040637923371399154 1103 72-73 -0.06928018729919216 1103 74-75 -0.09575576948003572 1103 76-77 -0.13621806608569642 1103 78-79 -0.1542005431050555 1103 80-81 -0.05705691274685876 1103 82-83 -0.09244007298118362 1103 84-85 -0.12243105057977743 1103 86-87 -0.13419994622453402 1103 88-89 0.0035143589946073917 1103 90-91 0.08472674690762716 1103 92-93 0.07870104625929031 1103 94-95 0.09080784241207596 1103 96-97 0.09199940865586953 1103 98-99 0.08178100926116016 1103 100 0.0602718905709807 1104 1 0.03730486878799866 1104 2 0.08312006364065638 1104 3 0.14946061031248803 1104 4 0.17417364888338227 1104 5 0.17812027883500292 1104 6 0.196078846882763 1104 7 0.14515742405392018 1104 8 0.08995577169263669 1104 9 0.18667587627286508 1104 10-11 0.14343998448335782 1104 12-13 0.2000794088322948 1104 14-15 0.1239022762074029 1104 16-17 0.16647744544771825 1104 18-19 0.17630447595431065 1104 20-21 0.1044204555052275 1104 22-23 0.13412952810893586 1104 24-25 0.10034168518568265 1104 26-27 0.1492711003615952 1104 28-29 0.22931898907967252 1104 30-31 0.16258678771560398 1104 32-33 0.21118083152727962 1104 34-35 0.20097604938760583 1104 36-37 0.2100354287810049 1104 38-39 0.15310821220007398 1104 40-41 0.14452869714159533 1104 42-43 0.08557492569490677 1104 44-45 0.10539803108412826 1104 46-47 0.09319791995535809 1104 48-49 0.03692939436842835 1104 50-51 0.11089182269258657 1104 52-53 0.02564392302078744 1104 54-55 -0.013168821404008213 1104 56-57 0.036621059344042806 1104 58-59 -0.006399213706288265 1104 60-61 -0.03584716794302523 1104 62-63 -0.030867986521293744 1104 64-65 0.0036038476222373106 1104 66-67 0.10111713970069047 1104 68-69 -0.05463993121273347 1104 70-71 0.03665362871531386 1104 72-73 0.05327859495292131 1104 74-75 0.022147359545911627 1104 76-77 -0.04598650352487965 1104 78-79 -0.056239362323076136 1104 80-81 0.03363128024675888 1104 82-83 0.06142692605337885 1104 84-85 0.07183047264373954 1104 86-87 0.10275014040259833 1104 88-89 0.32986014548703935 1104 90-91 0.2041171471569072 1104 92-93 0.20441156709203767 1104 94-95 0.2074652801134249 1104 96-97 0.24657043696282344 1104 98-99 0.31583507785834186 1104 100 0.31956014025234936 1105 1 -8.987166968168481E-4 1105 2 0.06217115303296694 1105 3 0.07024086284059905 1105 4 0.05463661707727141 1105 5 0.05388636078893683 1105 6 0.059474158823704215 1105 7 -0.08441023219901922 1105 8 0.062254653111764924 1105 9 -0.014333600592848939 1105 10-11 0.0752519230743971 1105 12-13 0.057376960288898715 1105 14-15 0.07877223411938417 1105 16-17 0.04905375940361267 1105 18-19 0.039914053096843816 1105 20-21 0.07848889325827457 1105 22-23 0.2030372404792402 1105 24-25 0.1664832520463051 1105 26-27 0.22135271533626621 1105 28-29 0.23944836030164396 1105 30-31 0.27120659262305225 1105 32-33 0.173868085543063 1105 34-35 0.2160658865907834 1105 36-37 0.22958240588211254 1105 38-39 0.18271129488842774 1105 40-41 0.1735584247464459 1105 42-43 0.18609184564293457 1105 44-45 0.14646697963694777 1105 46-47 0.1555796945255281 1105 48-49 0.09520488021668427 1105 50-51 0.1802049970895112 1105 52-53 0.09550714873308408 1105 54-55 0.04736554106138868 1105 56-57 0.1407365283382589 1105 58-59 0.020917275004329383 1105 60-61 0.030980918511609445 1105 62-63 0.06349885715985693 1105 64-65 0.14132364452636637 1105 66-67 0.021267307770294508 1105 68-69 -0.017305588049438825 1105 70-71 0.001977562862158777 1105 72-73 -0.061682247654843536 1105 74-75 0.005012145904494503 1105 76-77 0.0067030808677159825 1105 78-79 0.02092194447691753 1105 80-81 0.024058786696492973 1105 82-83 0.09270705617785069 1105 84-85 0.1041623324256733 1105 86-87 0.1250746795058575 1105 88-89 0.10596760410519579 1105 90-91 0.14601555685126755 1105 92-93 0.1100526090294025 1105 94-95 0.14971713029137845 1105 96-97 0.1852552805686507 1105 98-99 0.1649926113310265 1105 100 0.21597842170537263 1106 1 -0.06496681705523955 1106 2 -0.022954294995706448 1106 3 0.026486789852654624 1106 4 -0.034276709739280875 1106 5 -0.005324701446312474 1106 6 -0.011344919051822444 1106 7 -0.05179578123779294 1106 8 -0.029489400325836357 1106 9 -8.832525886504072E-5 1106 10-11 -0.017963497257959204 1106 12-13 0.008251634449862877 1106 14-15 -0.022018943788737033 1106 16-17 -0.0026731874846248616 1106 18-19 -0.002714856971579138 1106 20-21 -0.010454980967857352 1106 22-23 -0.028705591061985558 1106 24-25 0.06211802721737314 1106 26-27 0.05818881830140299 1106 28-29 0.10471391911558925 1106 30-31 0.0645228194070171 1106 32-33 0.08268406794775274 1106 34-35 0.04083067153701592 1106 36-37 0.05106843553345897 1106 38-39 0.07015427305722 1106 40-41 0.09745973624855964 1106 42-43 0.1544039994389621 1106 44-45 0.11121017774707553 1106 46-47 0.13575951694325283 1106 48-49 0.07162737125611329 1106 50-51 8.91702856861798E-4 1106 52-53 -0.002046003759581083 1106 54-55 0.08924603959511046 1106 56-57 0.09331676620075768 1106 58-59 0.0648851798593384 1106 60-61 0.014928254777487382 1106 62-63 0.051381718026590306 1106 64-65 0.03527831217054711 1106 66-67 0.13793295618997092 1106 68-69 0.061695104358506114 1106 70-71 0.08681281049982914 1106 72-73 0.15827437502355224 1106 74-75 0.08894624495955838 1106 76-77 0.05032478174747723 1106 78-79 0.09142027455185797 1106 80-81 0.08311202064891887 1106 82-83 0.07123222724334255 1106 84-85 0.0664770114742268 1106 86-87 0.04524411580432286 1106 88-89 0.08252246725280088 1106 90-91 0.10167090061145245 1106 92-93 0.09816966670291549 1106 94-95 0.04447112181956925 1106 96-97 0.060346922797389624 1106 98-99 0.06905338031114994 1106 100 0.04637920371905935 1107 1 0.04510104573178353 1107 2 0.07027698592731113 1107 3 0.028316934752425027 1107 4 0.07517234058548183 1107 5 -0.023151007642987054 1107 6 0.03146300587936679 1107 7 -0.09439242725547103 1107 8 -0.09416610133236958 1107 9 -0.028151799078585782 1107 10-11 -0.05243576478776646 1107 12-13 -0.13735944520416865 1107 14-15 -0.056024519674018336 1107 16-17 -0.05368295983863902 1107 18-19 0.06485824318495759 1107 20-21 -0.023206837170484107 1107 22-23 -0.0496480264787138 1107 24-25 -0.028981684222586068 1107 26-27 0.032904366457501055 1107 28-29 0.04362755111279171 1107 30-31 -0.008263235809074843 1107 32-33 0.046237046839578966 1107 34-35 0.0539177569491045 1107 36-37 -0.01777703319115176 1107 38-39 0.0303770267632828 1107 40-41 0.047554717857906326 1107 42-43 0.10129693173674781 1107 44-45 0.07678673912172229 1107 46-47 -0.007085217469764871 1107 48-49 -0.02607644951176269 1107 50-51 -0.05540708496558722 1107 52-53 -0.0700505824083919 1107 54-55 -0.014619613308376245 1107 56-57 -0.048912911819563476 1107 58-59 -0.015860456292081437 1107 60-61 0.09136004502354211 1107 62-63 0.1084237381159241 1107 64-65 0.027251121982907023 1107 66-67 0.09800593662178159 1107 68-69 0.005498199923245295 1107 70-71 0.08986978162410253 1107 72-73 0.04286772884623957 1107 74-75 0.12234239855651197 1107 76-77 0.00563271841638624 1107 78-79 0.11255333049945193 1107 80-81 0.028794559526581054 1107 82-83 0.07184822265342206 1107 84-85 0.06175422349402737 1107 86-87 0.0137208323985476 1107 88-89 0.0865982722489349 1107 90-91 0.03522368230962769 1107 92-93 0.029485239461266133 1107 94-95 -0.033223177311931806 1107 96-97 -0.04564701302828311 1107 98-99 -0.07200487351088825 1107 100 0.05701733176910295 1108 1 0.08229859562726816 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0.022606050590653126 2214 26-27 -0.010796025191481817 2214 28-29 0.04998839085245521 2214 30-31 0.032200767002301234 2214 32-33 0.011631099590445615 2214 34-35 -0.012771029723950278 2214 36-37 0.007904338820075907 2214 38-39 0.005770794094708265 2214 40-41 0.04238077700929921 2214 42-43 0.06212293558812121 2214 44-45 0.11142274460534196 2214 46-47 0.1633477816552471 2214 48-49 0.0894431877914883 2214 50-51 0.07032428004395541 2214 52-53 0.057584173473252065 2214 54-55 0.049531616180445326 2214 56-57 0.0674424692733524 2214 58-59 0.10747079883097399 2214 60-61 0.044915702259771706 2214 62-63 0.01770071716524768 2214 64-65 0.04802769475549695 2214 66-67 0.09139125197012987 2214 68-69 0.07489262907098748 2214 70-71 0.0675589455859793 2214 72-73 0.06164953193002276 2214 74-75 0.021316583260350797 2214 76-77 -0.02091320690896481 2214 78-79 -0.031159236072404894 2214 80-81 0.015501283027397506 2214 82-83 0.03169996509449646 2214 84-85 0.029925541159613545 2214 86-87 -0.04512779451097515 2214 88-89 -0.13448891714558897 2214 90-91 -0.24176580265991277 2214 92-93 -0.15155314320949032 2214 94-95 -0.1972861943559323 2214 96-97 -0.18256771191345322 2214 98-99 -0.20939749559682141 2214 100 -0.13679361531502465 2215 1 -0.2067274884575241 2215 2 -0.25201218202171205 2215 3 -0.2539696838595802 2215 4 -0.3302094943972378 2215 5 -0.23232470805351113 2215 6 -0.27147905206439304 2215 7 -0.18493301997974498 2215 8 -0.20829609223198986 2215 9 -0.3347313611188909 2215 10-11 -0.3569049046530779 2215 12-13 -0.33090342007167095 2215 14-15 -0.38377929874116035 2215 16-17 -0.36209742086744257 2215 18-19 -0.35981687866890866 2215 20-21 -0.3138045593071084 2215 22-23 -0.2567545768053989 2215 24-25 -0.22058823984614406 2215 26-27 -0.291709448283612 2215 28-29 -0.20747817255890055 2215 30-31 -0.24722402557045342 2215 32-33 -0.2365999346770451 2215 34-35 -0.3096802819751048 2215 36-37 -0.5011297086793434 2215 38-39 -0.31289855007548084 2215 40-41 -0.32709479101880845 2215 42-43 -0.35160137568801275 2215 44-45 -0.26419738185764885 2215 46-47 -0.3762231092637336 2215 48-49 -0.3227884796827851 2215 50-51 -0.24476789167697177 2215 52-53 -0.2847689626840477 2215 54-55 -0.27786275273831507 2215 56-57 -0.27534380411144355 2215 58-59 -0.2457446579548943 2215 60-61 -0.3636469331885124 2215 62-63 -0.32974958502006047 2215 64-65 -0.3271880563377536 2215 66-67 -0.3527036487828852 2215 68-69 -0.3472785270219134 2215 70-71 -0.41317306227411876 2215 72-73 -0.30831647408098206 2215 74-75 -0.2796102620320653 2215 76-77 -0.37855471970836874 2215 78-79 -0.31864284850227875 2215 80-81 -0.30363300734993715 2215 82-83 -0.3261934183404591 2215 84-85 -0.3321467098162074 2215 86-87 -0.39940862915482 2215 88-89 -0.2028331111149697 2215 90-91 -0.2502054384322321 2215 92-93 -0.24271582087155963 2215 94-95 -0.3337062869289831 2215 96-97 -0.40091361524388347 2215 98-99 -0.38402492920672415 2215 100 -0.3830679514140556 2216 1 0.007463746086109779 2216 2 -0.04674427913322532 2216 3 -0.13770060587185284 2216 4 -0.12391605540967987 2216 5 -0.1881997594484659 2216 6 -0.1579174004981354 2216 7 -0.05443236988048028 2216 8 -0.205647879469808 2216 9 -0.17708520971484631 2216 10-11 -0.20679935561040708 2216 12-13 -0.2514630836713607 2216 14-15 -0.3110629096512625 2216 16-17 -0.3111577932240621 2216 18-19 -0.37143206883511226 2216 20-21 -0.2514876386286673 2216 22-23 -0.25980109177574917 2216 24-25 -0.30693249277130974 2216 26-27 -0.3389669523639398 2216 28-29 -0.3033929374153459 2216 30-31 -0.33199901227518325 2216 32-33 -0.3509189928851839 2216 34-35 -0.3653725007720823 2216 36-37 -0.4333622471133225 2216 38-39 -0.3491184056664238 2216 40-41 -0.394769609617903 2216 42-43 -0.4536685926432469 2216 44-45 -0.3785893798960913 2216 46-47 -0.29793830796447907 2216 48-49 -0.2261997772041866 2216 50-51 -0.27356373048571214 2216 52-53 -0.2543266435991498 2216 54-55 -0.37973109658786086 2216 56-57 -0.3326435486587158 2216 58-59 -0.3454301931143249 2216 60-61 -0.26066294700837034 2216 62-63 -0.26765624455514114 2216 64-65 -0.27154862248705314 2216 66-67 -0.2795798182874023 2216 68-69 -0.25312095530458123 2216 70-71 -0.3119786907965292 2216 72-73 -0.319914949593354 2216 74-75 -0.282383747823431 2216 76-77 -0.30207433652649485 2216 78-79 -0.2692307873192803 2216 80-81 -0.3031804259540891 2216 82-83 -0.25858316486985444 2216 84-85 -0.278008606326857 2216 86-87 -0.33702342511928407 2216 88-89 -0.26127786157387334 2216 90-91 -0.29315264681399356 2216 92-93 -0.3404386178245886 2216 94-95 -0.28671204733383604 2216 96-97 -0.359456061290647 2216 98-99 -0.42303171876165635 2216 100 -0.4563866935380112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18185.0 3 3165.0 4 1914.0 5 1659.0 6 1417.0 7 1654.0 8 1903.0 9 2136.0 10 2320.0 11 2675.0 12 2907.0 13 3369.0 14 3571.0 15 3759.0 16 3772.0 17 3888.0 18 3989.0 19 3897.0 20 3921.0 21 4235.0 22 4762.0 23 6273.0 24 9630.0 25 13661.0 26 14940.0 27 13073.0 28 13156.0 29 16759.0 30 20350.0 31 24507.0 32 32113.0 33 43984.0 34 62372.0 35 93810.0 36 173165.0 37 386253.0 38 703471.0 39 352103.0 40 1084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84788773833149 20.162519886327658 13.300044132303384 23.68954824303747 2 15.140763770506227 20.05200168575291 41.46874381756926 23.338490726171607 3 18.01012726856694 25.333037310963892 30.269582778493813 26.387252641975355 4 11.759860432767216 16.694448006154637 40.2069321241651 31.338759436913044 5 12.271919015495445 38.171651052061264 36.64245751174313 12.913972420700162 6 30.56511118545216 39.077830059279165 17.872844763754888 12.48421399151379 7 26.84904570238344 32.68931798429815 22.980826653330936 17.48080965998747 8 24.311878048756803 37.572385971761335 20.61258221871319 17.503153760768672 9 24.342490211950185 17.110331703569827 21.645732565666005 36.901445518813986 10-11 23.23365223902485 27.273890490809165 29.70569611340365 19.78676115676233 12-13 23.92354232218467 24.523996814668063 29.666559841510313 21.88590102163695 14-15 21.200818204441855 26.78995575140667 26.4960091289279 25.51321691522358 16-17 20.865884697457744 30.254176816395862 27.029069763207108 21.850868722939282 18-19 20.737917161860807 29.244274058544388 29.038872183463205 20.978936596131597 20-21 21.989740581470645 28.635636219190364 28.360831232880834 21.013791966458157 22-23 21.525012394152 28.42315310140286 28.534566937889394 21.51726756655575 24-25 21.461393767089078 28.52630804520003 28.486414812408324 21.52588337530257 26-27 21.214319601390528 28.424815682938238 28.563014025237948 21.797850690433286 28-29 21.203009855450798 28.466694636412726 28.650076139864268 21.68021936827221 30-31 21.524877401171917 29.03039246678214 28.201105295385602 21.243624836660338 32-33 21.06450096539721 28.674968046712017 28.825435011479872 21.4350959764109 34-35 21.189745591756694 28.357114989301834 28.851102385546152 21.602037033395316 36-37 21.64033769959147 28.80149952757404 28.22849490819011 21.32966786464438 38-39 21.387264853390096 28.917977211117385 28.391614910903694 21.30314302458882 40-41 21.102403876499693 28.634655441131486 28.732320510392846 21.530620171975976 42-43 21.119658538788 28.403627078600458 28.80802226042778 21.668692122183764 44-45 21.255156950454918 28.655480994115845 28.698450523621343 21.39091153180789 46-47 21.27558284770278 28.93381384559922 28.511864517875374 21.278738788822626 48-49 21.18540280084954 28.65565387910306 28.617347264512027 21.54159605553537 50-51 21.280630204160904 28.490957201466266 28.636588740805475 21.591823853567355 52-53 21.24608577205605 28.552944006785673 28.596738206093463 21.604232015064817 54-55 21.430020997948755 28.99275388704803 28.365242932915802 21.211982182087414 56-57 21.228915071862385 28.667467254897534 28.40318574884979 21.700431924390294 58-59 21.038716901968144 28.272371158696014 28.871526340664943 21.817385598670896 60-61 21.413191398252483 28.72032182999542 28.388262293776684 21.478224477975417 62-63 21.223729048902847 28.739324486572798 28.490991811090442 21.54595465343391 64-65 21.191026362735766 28.386214892186874 28.70212786100412 21.720630884073245 66-67 21.012150427015147 28.54165746253331 28.478883250160337 21.967308860291208 68-69 21.523777804534173 28.64469038750597 28.42874038226258 21.402791425697274 70-71 21.4788758036788 28.452110866043256 28.427981689013258 21.64103164126468 72-73 21.146771040982326 28.619270344301246 28.642913806713967 21.59104480800246 74-75 21.170752595458094 28.63837491115435 28.535182876385118 21.655689617002434 76-77 21.24040766745739 28.60389140649351 28.36264478923814 21.79305613681096 78-79 21.39537017661998 28.677017846487868 28.46419900055007 21.46341297634208 80-81 21.254227512256506 28.461705026212275 28.58451286144441 21.699554600086806 82-83 21.124721476512512 28.465264276581742 28.766563636138542 21.643450610767204 84-85 21.098662083547257 28.47292063411147 28.64226301230864 21.786154270032636 86-87 21.318925773588276 28.59851574364292 28.468670014642537 21.61388846812626 88-89 21.053081434904776 28.443289397144433 28.593599125541715 21.91003004240908 90-91 21.027587935108244 28.327397476410038 28.69483280031927 21.95018178816245 92-93 21.284664751399998 28.569223506149395 28.456510477915455 21.689601264535156 94-95 20.977923797137173 28.294135128739796 28.705779157874534 22.022161916248496 96-97 20.98074331798653 28.27324278930565 28.76141181480883 21.98460207789899 98-99 21.071089586793164 28.314424670951183 28.608646241355455 22.005839500900198 100 21.198989633585892 28.28303152901922 28.471351951138274 22.046626886256615 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 229.0 1 211.0 2 203.5 3 184.5 4 141.0 5 113.0 6 108.5 7 108.5 8 105.5 9 115.5 10 122.5 11 132.5 12 139.0 13 159.0 14 200.0 15 241.5 16 336.5 17 498.5 18 709.0 19 1060.5 20 1551.5 21 2334.0 22 3603.0 23 5566.5 24 8063.0 25 11070.5 26 15195.0 27 20389.5 28 26637.0 29 33340.5 30 39535.5 31 45953.5 32 52954.5 33 59065.5 34 64585.0 35 69157.5 36 72085.0 37 75340.5 38 77913.5 39 78285.0 40 78257.0 41 77683.0 42 75992.5 43 74205.5 44 72145.0 45 69678.5 46 67416.5 47 65124.5 48 62657.5 49 60895.0 50 59128.5 51 56335.0 52 52739.5 53 49452.0 54 46890.5 55 44349.5 56 40937.0 57 36908.5 58 33529.5 59 30114.0 60 26249.0 61 22331.5 62 18752.0 63 15591.0 64 12752.5 65 10327.5 66 8075.5 67 6055.0 68 4524.0 69 3471.0 70 2539.0 71 1841.0 72 1397.5 73 1028.0 74 772.0 75 603.0 76 453.0 77 312.0 78 209.0 79 150.0 80 94.0 81 52.5 82 35.5 83 22.5 84 14.0 85 13.5 86 10.5 87 6.5 88 11.0 89 10.0 90 6.5 91 4.5 92 3.0 93 3.0 94 2.5 95 2.0 96 1.0 97 2.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33420688007876487 2 0.12433233873935456 3 0.06791914951048693 4 0.04204287596574816 5 0.04558690592590939 6 0.010923380014195539 7 0.008350317166407257 8 0.017283214600238274 9 0.019564987314314677 10-11 0.022720630429526722 12-13 0.017574504733950155 14-15 0.020123293403929113 16-17 0.020802970382590172 18-19 0.020608776960115583 20-21 0.02541506416636162 22-23 0.017623053089568803 24-25 0.02696861154615832 26-27 0.021506921539060548 28-29 0.02106998633849273 30-31 0.021506921539060548 32-33 0.026507402167781174 34-35 0.01633652166567466 36-37 0.010680638236102305 38-39 0.014030474773788939 40-41 0.010292251391153131 42-43 0.008884349078212372 44-45 0.009855316190585307 46-47 0.009442655167826811 48-49 0.005121851517767242 50-51 0.00737935005403432 52-53 0.008180397921741992 54-55 0.0037382233826358064 56-57 0.003228465648640015 58-59 0.0033255623598773083 60-61 0.003932416805110394 62-63 0.003155643115212045 64-65 0.0027915304480721935 66-67 0.0018933858691272268 68-69 0.0032041914708306916 70-71 0.0026701595590255763 72-73 0.001747740802271286 74-75 0.002427417780932342 76-77 0.004078061871966334 78-79 0.003349836537686632 80-81 0.0038838684494917476 82-83 0.0027429820924535467 84-85 0.0033741107154959555 86-87 0.002427417780932342 88-89 0.6002033205133309 90-91 0.6136026666640774 92-93 0.6101314592373442 94-95 0.5961980811747926 96-97 0.5948630013952797 98-99 0.5920714709472076 100 0.5936492925048136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2059802.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.63426922697131 #Duplication Level Percentage of deduplicated Percentage of total 1 82.30929892889668 62.254036750714334 2 12.210422190110728 18.470527186036392 3 3.021336341329994 6.855496987961558 4 1.0719024067167986 3.242902208586275 5 0.48530860982139384 1.835298102669924 6 0.26529626121765676 1.2039293307507097 7 0.15688795937321628 0.8306274310392785 8 0.10756179842975402 0.650828641677859 9 0.06954973017724944 0.4734308715200367 >10 0.293753655723424 3.6219254833034356 >50 0.006945730464316597 0.3510357054339369 >100 0.0017363877388829193 0.20996130030628066 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.825802674237621E-4 0.0 10-11 0.0 0.0 0.0 6.311286230424089E-4 0.0 12-13 0.0 0.0 0.0 6.311286230424089E-4 0.0 14-15 0.0 0.0 0.0 6.796769786610558E-4 0.0 16-17 0.0 0.0 0.0 7.282253342797026E-4 0.0 18-19 0.0 0.0 0.0 7.282253342797026E-4 0.0 20-21 0.0 0.0 0.0 7.52499512089026E-4 0.0 22-23 0.0 0.0 0.0 8.010478677076729E-4 0.0 24-25 0.0 0.0 0.0 8.738704011356432E-4 0.0 26-27 0.0 0.0 0.0 9.466929345636134E-4 0.0 28-29 0.0 0.0 0.0 0.001553547379796699 0.0 30-31 0.0 0.0 0.0 0.004417900361296863 0.0 32-33 0.0 0.0 0.0 0.012161363082471033 0.0 34-35 0.0 0.0 0.0 0.02106998633849273 0.0 36-37 0.0 0.0 0.0 0.037018121159218216 0.0 38-39 0.0 0.0 0.0 0.06655979555316482 0.0 40-41 0.0 0.0 0.0 0.10474307724723056 0.0 42-43 0.0 0.0 0.0 0.14151845662835555 0.0 44-45 0.0 0.0 0.0 0.18341568752724777 0.0 46-47 0.0 0.0 0.0 0.23531387968358125 0.0 48-49 0.0 0.0 0.0 0.2963391626962203 0.0 50-51 0.0 0.0 0.0 0.36311742584966905 0.0 52-53 0.0 0.0 0.0 0.42987141482530844 0.0 54-55 0.0 0.0 0.0 0.5039804796771729 0.0 56-57 0.0 0.0 0.0 0.5883089733867624 0.0 58-59 0.0 0.0 0.0 0.682250041508844 0.0 60-61 0.0 0.0 0.0 0.7850026361757101 0.0 62-63 0.0 0.0 0.0 0.8893816007558009 0.0 64-65 0.0 0.0 0.0 1.0013341088124004 0.0 66-67 0.0 0.0 0.0 1.1296716868902932 0.0 68-69 0.0 0.0 0.0 1.2764333659254627 0.0 70-71 0.0 0.0 0.0 1.4371041488453744 0.0 72-73 0.0 0.0 0.0 1.6079215380895833 0.0 74-75 0.0 0.0 0.0 1.7765542513309531 0.0 76-77 0.0 0.0 0.0 1.9547995389848152 0.0 78-79 0.0 0.0 0.0 2.1641157742346113 0.0 80-81 0.0 0.0 0.0 2.3946233667119463 0.0 82-83 0.0 0.0 0.0 2.6356902265363367 0.0 84-85 0.0 0.0 0.0 2.872120718399147 0.0 86-87 0.0 0.0 0.0 3.113673061779725 0.0 88 0.0 0.0 0.0 3.310852208124859 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1445 0.0 33.922165 1 TCAACGC 2110 0.0 28.50247 4 GTATCAA 2315 0.0 27.892513 1 ATCAACG 2140 0.0 27.890121 3 CAACGCA 2195 0.0 27.183355 5 GTACATG 4955 0.0 27.01424 1 TACATGG 4860 0.0 25.747715 2 ACATGGG 4795 0.0 25.384676 3 AACGCAG 2400 0.0 25.240698 6 GTACGAC 115 1.3785303E-4 24.519629 3 TATCAAC 2790 0.0 21.919594 2 ACGCAGA 2935 0.0 20.479755 7 CGCAGAG 2905 0.0 19.883482 8 CTTAGAC 545 0.0 19.833155 3 GAGTACA 3545 0.0 19.27835 1 GTGTAGG 690 0.0 19.126127 1 CATGGGG 3220 0.0 18.531168 4 GTACTAG 410 1.8189894E-12 18.393068 1 ACACCGT 185 1.6614204E-4 17.768436 6 GTGTAAG 1085 0.0 16.941542 1 >>END_MODULE