FastQCFastQC Report
Wed 25 May 2016
SRR1033182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059802
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.15525764126843258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28880.14020765102665209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.054.2183461
GTATCAA51800.041.1567081
ATCAACG57950.036.019483
TCAACGC57900.035.8881954
CAACGCA59350.034.8529745
AACGCAG61650.033.5404856
TATCAAC63550.033.067392
CGCAGAG69700.029.3956038
ACGCAGA70200.029.3215377
CGAACCG650.006147669628.9304075
GCAGAGT80050.025.1839929
TACATGG48200.024.6763782
GTACATG49300.024.2470551
TATACCG1602.0356201E-623.5059575
ACATGGG48800.023.1206113
GAGTACT55750.022.80085212-13
ATACCGT2807.6397555E-1121.8190126
CAGAGTA80150.021.42952210-11
AGAGTAC73300.020.86775210-11
ATACCGG1605.4201522E-520.5602236
Produced by FastQC (version 0.11.5)