##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033182_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2059802 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79534634882382 34.0 31.0 34.0 31.0 34.0 2 32.87943258623887 34.0 31.0 34.0 31.0 34.0 3 32.97242938884417 34.0 31.0 34.0 31.0 34.0 4 36.38701389745228 37.0 37.0 37.0 35.0 37.0 5 36.32652604473634 37.0 37.0 37.0 35.0 37.0 6 36.414420900649674 37.0 37.0 37.0 35.0 37.0 7 36.39003214872109 37.0 37.0 37.0 35.0 37.0 8 36.38121916572564 37.0 37.0 37.0 35.0 37.0 9 38.20431235623618 39.0 39.0 39.0 37.0 39.0 10-11 38.1897410527808 39.0 39.0 39.0 37.0 39.0 12-13 38.15307466445804 39.0 39.0 39.0 37.0 39.0 14-15 39.70280420156889 41.0 40.0 41.0 37.0 41.0 16-17 39.71994638319606 41.0 40.0 41.0 37.0 41.0 18-19 39.738546957426 41.0 40.0 41.0 37.0 41.0 20-21 39.735199062822545 41.0 40.0 41.0 37.0 41.0 22-23 39.6666987894953 41.0 40.0 41.0 37.0 41.0 24-25 39.62503022135137 41.0 40.0 41.0 37.0 41.0 26-27 39.53453026067554 41.0 40.0 41.0 37.0 41.0 28-29 39.448442374558326 41.0 39.0 41.0 36.5 41.0 30-31 39.36012102134089 41.0 39.0 41.0 36.0 41.0 32-33 39.39446752649041 41.0 39.0 41.0 36.0 41.0 34-35 39.343295132250574 41.0 39.0 41.0 36.0 41.0 36-37 39.263037175417836 41.0 39.0 41.0 35.5 41.0 38-39 39.174242718474886 41.0 39.0 41.0 35.0 41.0 40-41 39.06574321221166 41.0 39.0 41.0 35.0 41.0 42-43 38.962035428647994 41.0 39.0 41.0 35.0 41.0 44-45 38.836321403707736 41.0 39.0 41.0 35.0 41.0 46-47 38.64328367483865 41.0 39.0 41.0 35.0 41.0 48-49 38.47563333757322 41.0 39.0 41.0 35.0 41.0 50-51 38.35697168951191 41.0 39.0 41.0 35.0 41.0 52-53 38.23390257898575 41.0 38.0 41.0 34.5 41.0 54-55 38.11406751716913 40.0 38.0 41.0 34.0 41.0 56-57 37.97451138507488 40.0 38.0 41.0 34.0 41.0 58-59 37.802573742524764 40.0 37.0 41.0 34.0 41.0 60-61 37.62725033765381 40.0 37.0 41.0 34.0 41.0 62-63 37.40852640205223 40.0 36.5 41.0 33.0 41.0 64-65 37.17247652929748 39.0 36.0 41.0 33.0 41.0 66-67 36.91035910247684 39.0 35.5 41.0 33.0 41.0 68-69 36.600290464811664 39.0 35.0 41.0 32.5 41.0 70-71 36.271048382320245 37.5 35.0 40.5 32.0 41.0 72-73 35.91981607940957 37.0 35.0 40.0 32.0 41.0 74-75 35.580312330991035 37.0 35.0 39.0 32.0 41.0 76-77 35.231427341074536 36.0 35.0 39.0 31.0 41.0 78-79 34.87654128892 36.0 35.0 38.5 31.0 40.0 80-81 34.51587409857841 35.0 35.0 37.0 31.0 39.0 82-83 34.17823849088408 35.0 35.0 37.0 31.0 39.0 84-85 33.88812881043906 35.0 34.0 36.5 31.0 38.5 86-87 33.63969765055088 35.0 34.0 36.0 31.0 37.0 88-89 33.404619715875604 35.0 34.0 36.0 30.0 37.0 90-91 33.19345718666163 35.0 34.0 35.5 30.0 36.5 92-93 33.02137462727 35.0 34.0 35.0 30.0 36.0 94-95 32.868475707859304 35.0 34.0 35.0 30.0 36.0 96-97 32.744746825180286 35.0 34.0 35.0 30.0 36.0 98-99 32.637746249396784 35.0 34.0 35.0 30.0 36.0 100 32.54256768368999 35.0 34.0 35.0 30.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02895861850517889 1101 2 0.06872319186214071 1101 3 0.11979449792930552 1101 4 0.10017648644777921 1101 5 0.043774493460773556 1101 6 0.04859060335461152 1101 7 0.06757492796746334 1101 8 0.09485388381078508 1101 9 0.09912165397345518 1101 10-11 0.161708128389364 1101 12-13 0.11546748823216291 1101 14-15 0.1642209976722384 1101 16-17 0.1178306523245709 1101 18-19 0.1100265539190346 1101 20-21 0.1403785949514358 1101 22-23 0.16658972787877246 1101 24-25 0.12622576021454535 1101 26-27 0.15939144183390397 1101 28-29 0.23060336299679562 1101 30-31 0.23303102878846005 1101 32-33 0.2017420903440481 1101 34-35 0.18818119915302134 1101 36-37 0.21940046025262916 1101 38-39 0.13635688116903566 1101 40-41 0.14794092461920627 1101 42-43 0.12275735846655067 1101 44-45 0.1395435280606776 1101 46-47 0.08810414956568025 1101 48-49 0.04195215144429909 1101 50-51 0.11471384525096084 1101 52-53 0.15126229979014738 1101 54-55 0.12710728094901924 1101 56-57 0.1278380488957751 1101 58-59 0.0932748347818233 1101 60-61 0.09577363430584995 1101 62-63 0.09135802731627507 1101 64-65 0.10175476841809683 1101 66-67 0.10154103137974602 1101 68-69 0.18568626815213918 1101 70-71 0.18572943905362393 1101 72-73 0.19824331005257534 1101 74-75 0.09508054543676536 1101 76-77 0.10801507721269132 1101 78-79 0.08107310081307872 1101 80-81 0.05510557153674256 1101 82-83 0.12250486403382155 1101 84-85 0.1758308519414129 1101 86-87 0.018388151005417797 1101 88-89 0.04442393332205796 1101 90-91 0.035615077158837494 1101 92-93 0.04815098389973116 1101 94-95 0.036874137290467957 1101 96-97 0.12088736561598523 1101 98-99 0.16638075767273364 1101 100 0.13273719536655193 1102 1 0.18652983216570362 1102 2 0.1996601057414793 1102 3 0.171309619842404 1102 4 0.10506408152957647 1102 5 0.11029104811326818 1102 6 0.09974509824884592 1102 7 0.10169122081939719 1102 8 0.11616751046494755 1102 9 0.14166831569065863 1102 10-11 0.12731424227911248 1102 12-13 0.19354242375889186 1102 14-15 0.20817312789522902 1102 16-17 0.18088502478470758 1102 18-19 0.195782112296655 1102 20-21 0.20173728777211153 1102 22-23 0.2232216017716553 1102 24-25 0.19805118417516354 1102 26-27 0.21467930848494632 1102 28-29 0.22027217226010976 1102 30-31 0.2650779900784528 1102 32-33 0.19398209698675117 1102 34-35 0.21760038613853538 1102 36-37 0.23928012642434737 1102 38-39 0.29120751808682144 1102 40-41 0.25382118016781163 1102 42-43 0.18624603005662976 1102 44-45 0.21685365752821895 1102 46-47 0.2593449995708852 1102 48-49 0.3215341497250179 1102 50-51 0.40691778919177324 1102 52-53 0.45777003007044215 1102 54-55 0.40062293037070873 1102 56-57 0.39042132687325903 1102 58-59 0.43152222003242 1102 60-61 0.35250018296688523 1102 62-63 0.3451718819062535 1102 64-65 0.2975500039083627 1102 66-67 0.32322774011684174 1102 68-69 0.3894051223513628 1102 70-71 0.3625559375687786 1102 72-73 0.3947634628346961 1102 74-75 0.36075357476885017 1102 76-77 0.3346874748369473 1102 78-79 0.38179411063385515 1102 80-81 0.3931836330403371 1102 82-83 0.33354615623451167 1102 84-85 0.3605939940691627 1102 86-87 0.3992220867405436 1102 88-89 0.38288378731289185 1102 90-91 0.3496186394699734 1102 92-93 0.3748053908758635 1102 94-95 0.3763763905987929 1102 96-97 0.38288818618491405 1102 98-99 0.3850908689054151 1102 100 0.3226036575422313 1103 1 0.060621313727963866 1103 2 0.052707028564022096 1103 3 0.08342069733726021 1103 4 0.0406287525632294 1103 5 -0.030312074446136705 1103 6 0.05934500224707051 1103 7 0.07996495565592454 1103 8 0.05427099068569419 1103 9 0.06995506705465715 1103 10-11 0.07767221632892785 1103 12-13 0.06395136161807358 1103 14-15 0.09611372562354603 1103 16-17 0.12005601661282839 1103 18-19 0.03595733117366251 1103 20-21 0.013658433414150295 1103 22-23 0.056908296691609905 1103 24-25 0.0028181257915420588 1103 26-27 0.06545263875372598 1103 28-29 0.017042158102185567 1103 30-31 0.06115004769827692 1103 32-33 0.08870008395145845 1103 34-35 0.09749790346067755 1103 36-37 0.030233131678642167 1103 38-39 0.05604479585795019 1103 40-41 0.08396917769427859 1103 42-43 -0.023412311504877437 1103 44-45 -0.03300972756633769 1103 46-47 -0.05520954984227444 1103 48-49 -0.07678644461041273 1103 50-51 -0.11983899249721475 1103 52-53 -0.10411789020118078 1103 54-55 -0.08396499294982362 1103 56-57 -0.041088871751910006 1103 58-59 -0.061868862903573074 1103 60-61 -0.03402599119815619 1103 62-63 -0.1412661082168185 1103 64-65 -0.09193127379085553 1103 66-67 -0.05845652036067861 1103 68-69 -0.04679571680422612 1103 70-71 -0.059601123838739056 1103 72-73 0.015613711790443574 1103 74-75 -0.04161328335413117 1103 76-77 0.004948931140113189 1103 78-79 0.020602085973600026 1103 80-81 0.051808196517605154 1103 82-83 0.021367220806645548 1103 84-85 0.0791883967278153 1103 86-87 -0.008960383866728705 1103 88-89 0.048246846798811305 1103 90-91 0.015864321149038574 1103 92-93 -0.059538345516386926 1103 94-95 0.09958227922891894 1103 96-97 0.08735418412259577 1103 98-99 0.1379582183729795 1103 100 0.13529191835435483 1104 1 0.06434205712581331 1104 2 0.08388078401458898 1104 3 0.0876513801743215 1104 4 0.034587190309004257 1104 5 0.025650744398973302 1104 6 0.010206737330094029 1104 7 0.04035386112566641 1104 8 0.060481489932044497 1104 9 0.13568332001464967 1104 10-11 0.1045248763670088 1104 12-13 0.10296898675189681 1104 14-15 0.15882951916752575 1104 16-17 0.0950342581539374 1104 18-19 0.10270133344117482 1104 20-21 0.10194377925633091 1104 22-23 0.06328835862266402 1104 24-25 0.12919888849825867 1104 26-27 0.025150591925644505 1104 28-29 0.07841878185260498 1104 30-31 0.12113994902030356 1104 32-33 0.09756871291514102 1104 34-35 0.18633880172995276 1104 36-37 0.21608384518715695 1104 38-39 0.1703827912265794 1104 40-41 0.23280861184295532 1104 42-43 0.18151620624821163 1104 44-45 0.2229215563735778 1104 46-47 0.23361289049637435 1104 48-49 0.2413262914242864 1104 50-51 0.28292281447787815 1104 52-53 0.2624135017332634 1104 54-55 0.18128867220078604 1104 56-57 0.25041416066709843 1104 58-59 0.21146393233415495 1104 60-61 0.2746422910067068 1104 62-63 0.25977222859223303 1104 64-65 0.2667860939956199 1104 66-67 0.2863010206425187 1104 68-69 0.3553088627022163 1104 70-71 0.370404338457341 1104 72-73 0.3610851680085361 1104 74-75 0.3737138693880624 1104 76-77 0.28782284970872496 1104 78-79 0.2617655863230439 1104 80-81 0.3071596511589618 1104 82-83 0.24946382023014024 1104 84-85 0.18054402223580013 1104 86-87 0.1826042167005184 1104 88-89 0.17378117260568615 1104 90-91 0.24163847491549006 1104 92-93 0.2059678491528345 1104 94-95 0.1134281673963784 1104 96-97 0.16633942340932606 1104 98-99 0.17549032422264332 1104 100 0.18891226938039551 1105 1 0.029738668428379356 1105 2 0.07874383318318223 1105 3 0.05640644109367088 1105 4 0.04142072607911729 1105 5 0.06933612940547107 1105 6 0.03002541518257118 1105 7 0.030360546247038656 1105 8 -0.007176646143129517 1105 9 0.036801665494188285 1105 10-11 0.012466576930066253 1105 12-13 0.030376042379636203 1105 14-15 0.016905569484833904 1105 16-17 0.05385455785452109 1105 18-19 0.055453061215501975 1105 20-21 0.09439165178707043 1105 22-23 0.16289949170365503 1105 24-25 0.09190956397798544 1105 26-27 0.05949104820871298 1105 28-29 0.08787822858819538 1105 30-31 0.040766723872948774 1105 32-33 0.04348196169581087 1105 34-35 0.07406589577360023 1105 36-37 -0.022853043971643672 1105 38-39 -0.016856924514527805 1105 40-41 0.04844255672365705 1105 42-43 0.12279936115471202 1105 44-45 0.21976866246927784 1105 46-47 0.1940829322077633 1105 48-49 0.03778788493124807 1105 50-51 0.03502874540456702 1105 52-53 0.025770268300128407 1105 54-55 0.009127730257766586 1105 56-57 -0.0205094453438619 1105 58-59 0.006647290619000046 1105 60-61 -0.025350117614273415 1105 62-63 -0.07409656177742363 1105 64-65 -0.08065690592905383 1105 66-67 -0.11302670095513179 1105 68-69 -0.12596767770039463 1105 70-71 -0.03221050910305934 1105 72-73 -0.06405147681378054 1105 74-75 -0.022514980676902496 1105 76-77 -0.0202251301605898 1105 78-79 -0.001618191429621163 1105 80-81 0.0018641640866690068 1105 82-83 0.04110965474043127 1105 84-85 0.09187347866952678 1105 86-87 0.07970607535257557 1105 88-89 0.056790724796712766 1105 90-91 0.04009136364117438 1105 92-93 0.08533536370003958 1105 94-95 0.0073762494256470745 1105 96-97 -0.007640860190463172 1105 98-99 0.08934782756520576 1105 100 0.09497077831586154 1106 1 -0.022222475266637787 1106 2 -0.0181729149432428 1106 3 -0.01625566382649879 1106 4 0.04000536276858924 1106 5 -0.009802026345589354 1106 6 0.013539996479408956 1106 7 -0.018963337754264842 1106 8 0.05041540338440598 1106 9 0.06252258436026636 1106 10-11 0.032429309090375114 1106 12-13 0.06242536626363204 1106 14-15 0.0948014639129866 1106 16-17 0.04393174097605623 1106 18-19 0.007839832150665416 1106 20-21 0.02927984826801122 1106 22-23 0.04535305964109426 1106 24-25 0.05431588525527076 1106 26-27 -0.03478844022400551 1106 28-29 -0.06603998581389448 1106 30-31 0.018716585391359786 1106 32-33 0.10633834814091614 1106 34-35 0.12920906129983223 1106 36-37 0.10049230467612347 1106 38-39 0.07614466395076391 1106 40-41 0.14144414518895587 1106 42-43 0.1266025136473914 1106 44-45 0.20860358426790526 1106 46-47 0.21895361484054376 1106 48-49 0.16991561383316878 1106 50-51 0.10741592363851993 1106 52-53 0.061790755429484534 1106 54-55 0.060182634135252044 1106 56-57 0.09841162014978266 1106 58-59 0.05307426194516296 1106 60-61 0.06625647248022659 1106 62-63 0.1305150788221212 1106 64-65 0.05507032606833917 1106 66-67 0.03767385297123127 1106 68-69 0.0681458364736045 1106 70-71 0.032132517941576566 1106 72-73 0.10932543749054702 1106 74-75 0.13997178211227634 1106 76-77 0.08510742930504023 1106 78-79 0.08404692750162468 1106 80-81 0.13438900931312503 1106 82-83 0.08936437940644026 1106 84-85 0.06067948225375375 1106 86-87 0.11913259865006154 1106 88-89 0.08221635203685196 1106 90-91 0.06126071847987902 1106 92-93 0.04473276513373037 1106 94-95 0.0038327499144088506 1106 96-97 0.011461453266683463 1106 98-99 -0.008444612969164211 1106 100 0.07709036124189339 1107 1 -0.014914102893655468 1107 2 -0.012178227903653749 1107 3 0.05246069000651943 1107 4 1.8820823543563847E-4 1107 5 -0.08079737539243581 1107 6 -0.10040225207286113 1107 7 -0.0563568883950083 1107 8 -0.04121391386544104 1107 9 -0.04550197657867017 1107 10-11 -0.02098951238644986 1107 12-13 -0.021005390124265944 1107 14-15 0.038210172172490786 1107 16-17 0.053501267232398675 1107 18-19 0.0562419532031484 1107 20-21 -0.019793200712079795 1107 22-23 0.037454045327656615 1107 24-25 -0.008499213768807579 1107 26-27 0.004143690415858714 1107 28-29 -0.017467902613198305 1107 30-31 0.01757849631053432 1107 32-33 0.042737291558538004 1107 34-35 0.04718917854554405 1107 36-37 0.021539722237434944 1107 38-39 0.06681130056887952 1107 40-41 0.10019358060474559 1107 42-43 0.053877025053161276 1107 44-45 0.06043039565593489 1107 46-47 0.0324267303030652 1107 48-49 -0.043755916004577955 1107 50-51 0.03284573537492719 1107 52-53 0.10606730356398941 1107 54-55 0.023709320540071133 1107 56-57 0.05377261130141875 1107 58-59 0.005890515960636833 1107 60-61 0.03472849398747968 1107 62-63 -0.04308623129495004 1107 64-65 -0.016850424619725857 1107 66-67 -9.704688375435921E-4 1107 68-69 0.0026460867217394934 1107 70-71 0.011047821389745138 1107 72-73 0.04981849674348382 1107 74-75 -0.0018625695854197488 1107 76-77 -0.0502519279563316 1107 78-79 -0.014122645893387187 1107 80-81 -0.04277117672499742 1107 82-83 0.002257369923306385 1107 84-85 -0.020063806354180258 1107 86-87 0.004117053217122191 1107 88-89 0.11856477631872053 1107 90-91 0.08211019117466378 1107 92-93 0.0909610609204492 1107 94-95 0.06857799919065855 1107 96-97 0.04790565732267282 1107 98-99 0.029225478293930962 1107 100 0.05007107476365036 1108 1 0.049709674041210405 1108 2 0.050622419755391945 1108 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0.026067100569093782 2214 7 0.043017418682417485 2214 8 -0.010053514385262474 2214 9 -0.016920966068994403 2214 10-11 -0.049675592926462286 2214 12-13 -0.021453543237520023 2214 14-15 -0.048034751598223124 2214 16-17 -0.08986371198104592 2214 18-19 -0.07373569488717635 2214 20-21 -0.12773513034369444 2214 22-23 -0.15596413785275587 2214 24-25 -0.1068152390890802 2214 26-27 -0.08195491129647792 2214 28-29 -0.08844114721676277 2214 30-31 -0.023750996347501996 2214 32-33 -0.02315044762517715 2214 34-35 -0.08767248668064553 2214 36-37 -0.07707090945821449 2214 38-39 -0.07352858938408957 2214 40-41 -0.0182824223101008 2214 42-43 -0.044076874356107965 2214 44-45 -0.023285801094388603 2214 46-47 -4.0622618968200186E-5 2214 48-49 0.010015099116408521 2214 50-51 0.15763316478935963 2214 52-53 0.06322281345242686 2214 54-55 0.06238985323977175 2214 56-57 0.057457465429799015 2214 58-59 -0.011894638125724555 2214 60-61 -0.03534848898636511 2214 62-63 -0.08748884395312473 2214 64-65 0.0039636986475741764 2214 66-67 -0.0030437734683843587 2214 68-69 0.06605139035004015 2214 70-71 0.11470988655992187 2214 72-73 -0.002952658411409459 2214 74-75 0.05512311573786377 2214 76-77 0.07966582664895583 2214 78-79 -0.09571188807802145 2214 80-81 -0.07038158276090911 2214 82-83 -0.07095922686019662 2214 84-85 -0.12546532460720528 2214 86-87 -0.1203074029473612 2214 88-89 -0.2383041100192287 2214 90-91 -0.147640949834134 2214 92-93 -0.09453900731263332 2214 94-95 -0.1515531242826853 2214 96-97 -0.2744730104285793 2214 98-99 -0.3228732465055373 2214 100 -0.5203233481405434 2215 1 -0.03244754414664186 2215 2 -0.05939077050508956 2215 3 -0.07758616858490086 2215 4 -0.0324577438718876 2215 5 -0.07805000341375035 2215 6 -0.11287050923877473 2215 7 -0.06813356100443002 2215 8 -0.07198827327616897 2215 9 -0.10507256707142432 2215 10-11 -0.13040083766237842 2215 12-13 -0.14164563082815818 2215 14-15 -0.1531198777787992 2215 16-17 -0.13217530323267823 2215 18-19 -0.13552047592545335 2215 20-21 -0.18265162434011728 2215 22-23 -0.14215926364686737 2215 24-25 -0.09994903300850666 2215 26-27 -0.06854103485207474 2215 28-29 -0.12960263575809705 2215 30-31 -0.13470517655279934 2215 32-33 -0.1490404301748285 2215 34-35 -0.08999454219474501 2215 36-37 -0.019207551321798633 2215 38-39 -0.08652508445072016 2215 40-41 -0.08400460506011598 2215 42-43 -0.059947028317516526 2215 44-45 -0.02832655820755292 2215 46-47 -0.08968471878316109 2215 48-49 -0.11311983933173053 2215 50-51 -0.10521773730735617 2215 52-53 -0.1086449682131132 2215 54-55 -0.1483447855499378 2215 56-57 -0.3148546014701381 2215 58-59 -0.26132519793403475 2215 60-61 -0.27548190833275754 2215 62-63 -0.2887506496924104 2215 64-65 -0.23437132422813534 2215 66-67 -0.2653961366972055 2215 68-69 -0.2644158895065516 2215 70-71 -0.19287863604674982 2215 72-73 -0.22443271239749407 2215 74-75 -0.23010042409619302 2215 76-77 -0.29240165297992604 2215 78-79 -0.25525131188956607 2215 80-81 -0.17932243292277406 2215 82-83 -0.06459869437193078 2215 84-85 -0.08349872700863159 2215 86-87 -0.19598074256321496 2215 88-89 -0.22997260991370183 2215 90-91 -0.23622553942397673 2215 92-93 -0.19859509813767318 2215 94-95 -0.13534072650990936 2215 96-97 -0.20770455007237842 2215 98-99 -0.25398510412022546 2215 100 -0.2645083991092321 2216 1 -0.01685593436076971 2216 2 -0.12371021791098968 2216 3 -0.09027714306103007 2216 4 -0.03369587924765227 2216 5 -0.0029388666032303945 2216 6 -0.03099351685898455 2216 7 -0.03284930903001282 2216 8 -0.08594309798010613 2216 9 -0.13441210125174052 2216 10-11 -0.13047853821528577 2216 12-13 -0.08957107471515258 2216 14-15 -0.20560298729098037 2216 16-17 -0.17071262298894396 2216 18-19 -0.10351215710132067 2216 20-21 -0.16348568487094184 2216 22-23 -0.16411429156763546 2216 24-25 -0.27498040512799093 2216 26-27 -0.2813491578541303 2216 28-29 -0.21711726194894254 2216 30-31 -0.21020961160027696 2216 32-33 -0.22514311777997165 2216 34-35 -0.21097902905000865 2216 36-37 -0.2672590605334193 2216 38-39 -0.25893503590386757 2216 40-41 -0.3561470734023473 2216 42-43 -0.3654947226960772 2216 44-45 -0.23139115002389588 2216 46-47 -0.2978527704841909 2216 48-49 -0.2522770121213611 2216 50-51 -0.18799123977862564 2216 52-53 -0.13812503058483117 2216 54-55 -0.14397326070369587 2216 56-57 -0.04403418927078917 2216 58-59 -0.05072507722712771 2216 60-61 -0.07552738887106614 2216 62-63 -0.09646728759087608 2216 64-65 -0.08908637169775346 2216 66-67 -0.09634729015426302 2216 68-69 -0.07144444661422966 2216 70-71 -0.03383074720830592 2216 72-73 0.0301722268556901 2216 74-75 0.09204029980607942 2216 76-77 -0.007697409918137055 2216 78-79 -0.026477393254694448 2216 80-81 -0.02739053863889751 2216 82-83 -0.0563482230951422 2216 84-85 -0.02522345132456394 2216 86-87 -0.06467796282941407 2216 88-89 0.004748354751868078 2216 90-91 -0.03600643498470646 2216 92-93 0.0013266175127739643 2216 94-95 -0.029603096674229334 2216 96-97 -0.051842349153488954 2216 98-99 0.004963803387759924 2216 100 0.07858791371458551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 10.0 4 9.0 5 19.0 6 49.0 7 80.0 8 203.0 9 391.0 10 736.0 11 1292.0 12 1992.0 13 3051.0 14 3653.0 15 4955.0 16 6111.0 17 8527.0 18 9826.0 19 5429.0 20 3544.0 21 3258.0 22 3370.0 23 3629.0 24 4192.0 25 4965.0 26 6372.0 27 8332.0 28 10641.0 29 13828.0 30 17949.0 31 23208.0 32 29805.0 33 39339.0 34 54865.0 35 84409.0 36 152511.0 37 365648.0 38 750535.0 39 430850.0 40 2209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.758722900757796 20.12599948006385 13.447249562063126 23.66802805711523 2 15.392177805396692 20.194146947732282 40.914854984512914 23.498820262358116 3 18.052414746660116 25.385449669434244 30.58007517227384 25.982060411631796 4 11.90829784847939 16.838244896710798 39.88083303273766 31.372624222072155 5 12.005606637824018 37.981522961765684 36.621097354622755 13.391773045787541 6 30.477458744801076 38.9886207454021 17.600195358677855 12.933725151118965 7 26.48550603261901 32.97731389573044 22.87768380055726 17.65949627109329 8 24.630678908026198 36.93778937980661 20.472736823554598 17.958794888612594 9 24.59148906834554 16.242854881886476 21.795923677952885 37.369732371815104 10-11 23.394963417378328 26.906511563438883 29.48995516289407 20.20856985628871 12-13 24.41203086510257 24.162370946333677 29.819638004041167 21.60596018452259 14-15 21.409068841635108 26.288207592970192 27.162879891251578 25.13984367414312 16-17 20.479541237458747 29.94867953327553 27.609377017791033 21.962402211474696 18-19 20.20415093799178 28.387471693240045 30.536661456612645 20.871715912155526 20-21 21.456868184417726 27.724169604651323 30.13483820289523 20.684124008035724 22-23 20.99745751644458 27.573566715554982 30.253588096302693 21.175387671697745 24-25 20.946076829752 27.65012251183488 30.235153784273333 21.16864687413978 26-27 20.697502576752765 27.644272666321317 30.330892437625106 21.32733231930081 28-29 20.68425409603251 27.658494427356565 30.405124258659992 21.252127217950928 30-31 21.03661897682885 28.233220936258085 29.83894663073457 20.891213456178495 32-33 20.595011624166126 27.890229023387942 30.439630211905815 21.075129140540113 34-35 20.71819877516789 27.612674890164772 30.475675024681664 21.19345130998567 36-37 21.187395017969866 28.06484399166505 29.78753263881908 20.960228351546 38-39 21.051418848260337 28.15832225955164 29.81340445157526 20.97685444061276 40-41 20.798774455884345 27.94982145692894 30.027700521618694 21.22370356556802 42-43 20.847658283414525 27.872128211702368 29.911539791312187 21.368673713570924 44-45 21.03086841343022 28.060128082829195 29.71862032596854 21.19038317777204 46-47 21.114004763804218 28.39770651790481 29.36755898369911 21.120729734591865 48-49 21.109549300219705 28.2452204945195 29.20862313826882 21.43660706699198 50-51 21.15428639402028 28.171356228250012 29.168575385597638 21.50578199213207 52-53 21.14452939612826 28.24099035402559 29.07908679434909 21.535393455497065 54-55 21.286317771365844 28.658601418361208 28.853241319977997 21.20183949029495 56-57 21.14982585163744 28.303963902205037 28.89030061104859 21.65590963510893 58-59 20.870851553347766 27.922245951867602 29.440055889545324 21.766846605239305 60-61 21.281428924793904 28.308856281949613 28.973561094802786 21.4361536984537 62-63 21.084569274097507 28.35087657972965 28.981263414745456 21.58329073142739 64-65 21.031416488710537 28.04189674328753 29.198625742542532 21.7280610254594 66-67 20.896528504490387 28.165280966817242 28.99089931276958 21.947291215922792 68-69 21.489918207185 28.25093972528586 28.856369195216473 21.402772872312664 70-71 21.41219496032005 28.06885738788864 28.904062470339863 21.61488518145144 72-73 21.174732830449745 28.180115667271505 29.05469617618322 21.59045532609553 74-75 21.232107082838844 28.230568332622504 28.895466694760163 21.64185788977849 76-77 21.27009333702626 28.30324981606166 28.725913544246062 21.70074330266602 78-79 21.373855684637576 28.34129890671504 28.781171389573217 21.503674019074168 80-81 21.2508086392407 28.117123732423856 28.921769842762906 21.710297785572543 82-83 21.099363430870692 28.27826790277853 29.002273045045325 21.620095621305452 84-85 21.119568539273477 28.122479267946034 28.89881922629623 21.859132966484264 86-87 21.21692456399872 28.336866849629505 28.721564579844927 21.72464400652685 88-89 20.939415536056377 28.158094807170787 28.85619588678912 22.046293769983716 90-91 20.9019847538744 28.048424071828265 28.965939444665068 22.083651729632265 92-93 21.059864006346242 28.27558182776791 28.686567932257567 21.97798623362828 94-95 20.795712403425185 27.971159363861187 28.95394800082726 22.279180231886368 96-97 20.72565712626748 27.9628575950504 28.96928928120276 22.34219599747937 98-99 20.765151213563247 28.0198776387245 28.879669016730737 22.33530213098152 100 20.935895780274027 27.943996558892554 28.71154606122336 22.40856159961006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 30.0 1 26.0 2 88.5 3 162.5 4 116.5 5 81.5 6 170.0 7 413.0 8 750.0 9 928.0 10 774.0 11 611.0 12 655.0 13 782.5 14 951.5 15 1090.0 16 1221.5 17 1421.0 18 1696.5 19 2078.0 20 2608.5 21 3348.0 22 4552.5 23 6413.5 24 8708.0 25 11530.5 26 15455.5 27 20467.5 28 26681.5 29 33202.5 30 39088.5 31 45324.0 32 51846.0 33 58028.5 34 63903.0 35 68407.5 36 71385.5 37 74487.5 38 76867.0 39 77421.5 40 77592.0 41 77451.5 42 75794.0 43 73659.5 44 71748.5 45 69422.5 46 67577.5 47 65238.0 48 62522.5 49 60993.0 50 59082.5 51 56091.5 52 52321.5 53 48892.0 54 46417.0 55 44021.5 56 40817.5 57 37125.5 58 33882.5 59 30416.0 60 26315.0 61 22208.5 62 18657.5 63 15382.0 64 12423.5 65 10110.5 66 7950.0 67 5890.5 68 4392.5 69 3336.5 70 2434.0 71 1701.5 72 1185.5 73 857.0 74 684.5 75 505.0 76 322.0 77 224.5 78 171.5 79 116.0 80 61.0 81 28.5 82 11.5 83 3.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09015429638382719 2 0.0010680638236102305 3 0.0 4 1.4564506685594053E-4 5 0.03951836147357853 6 4.854835561864684E-5 7 0.004029513516347688 8 4.369352005678215E-4 9 1.9419342247458735E-4 10-11 6.068544452330855E-4 12-13 0.0 14-15 9.709671123729368E-5 16-17 0.0 18-19 1.2137088904661711E-4 20-21 0.0 22-23 2.4274177809323422E-4 24-25 4.854835561864684E-5 26-27 0.0018933858691272268 28-29 0.009078542500686959 30-31 0.035585944668468136 32-33 0.0249781289657938 34-35 0.028206594614433814 36-37 0.025293693277315007 38-39 0.02468683883208192 40-41 0.030828205817840747 42-43 0.022235146873340255 44-45 0.02094861544944611 46-47 0.015219909486445783 48-49 0.011020476725432833 50-51 0.008471688055453874 52-53 0.006529753830708001 54-55 0.004903383917483331 56-57 0.0031070947595933976 58-59 0.001213708890466171 60-61 7.52499512089026E-4 62-63 0.0016991924466526395 64-65 0.0034712074267332495 66-67 6.068544452330855E-4 68-69 0.0011894347126568477 70-71 4.1266102275849814E-4 72-73 7.039511564703793E-4 74-75 6.554028008517324E-4 76-77 4.1266102275849814E-4 78-79 2.427417780932342E-5 80-81 7.039511564703793E-4 82-83 4.8548355618646845E-4 84-85 4.1266102275849814E-4 86-87 1.4564506685594053E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2059802.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.90415128659646 #Duplication Level Percentage of deduplicated Percentage of total 1 81.40600126327021 60.16241432997583 2 12.73551347142031 18.824146286086673 3 3.1977940979122774 7.089907763864825 4 1.1544722967303511 3.4128118109497736 5 0.5251045108198132 1.9403701604450845 6 0.28393757341586573 1.2590499229005137 7 0.17388804282424378 0.8995733756669152 8 0.10882128505681961 0.6433875771232827 9 0.08380535125884614 0.5574207022254108 >10 0.3172816705482842 3.8722886222803115 >50 0.009697519084943765 0.47703948677499713 >100 0.003420074036797838 0.454776982915041 >500 6.571090532030175E-5 0.040744429892074975 >1k 1.9713271596090526E-4 0.3660685488992386 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3198 0.15525764126843258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2888 0.14020765102665209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.854835561864684E-5 0.0 5 0.0 0.0 0.0 4.854835561864684E-5 0.0 6 0.0 0.0 0.0 4.854835561864684E-5 0.0 7 0.0 0.0 0.0 4.854835561864684E-5 0.0 8 0.0 0.0 0.0 4.854835561864684E-5 0.0 9 0.0 0.0 0.0 2.427417780932342E-4 0.0 10-11 0.0 0.0 0.0 2.427417780932342E-4 0.0 12-13 0.0 0.0 0.0 2.427417780932342E-4 0.0 14-15 0.0 0.0 0.0 3.398384893305279E-4 0.0 16-17 0.0 0.0 0.0 3.398384893305279E-4 0.0 18-19 0.0 0.0 0.0 3.6411266713985127E-4 0.0 20-21 0.0 0.0 0.0 3.883868449491747E-4 0.0 22-23 0.0 0.0 0.0 4.61209378377145E-4 0.0 24-25 0.0 0.0 0.0 5.82580267423762E-4 0.0 26-27 0.0 0.0 0.0 7.039511564703792E-4 0.0 28-29 0.0 0.0 0.0 0.0013108056017034648 0.0 30-31 0.0 0.0 0.0 0.004175158583203628 0.0 32-33 0.0 0.0 0.0 0.0119186213043778 0.0 34-35 0.0 0.0 0.0 0.02087579291601814 0.0 36-37 0.0 0.0 0.0 0.03694529862579025 0.0 38-39 0.0 0.0 0.0 0.06660834390878347 0.0 40-41 0.0 0.0 0.0 0.1048887223140865 0.0 42-43 0.0 0.0 0.0 0.1419068434733047 0.0 44-45 0.0 0.0 0.0 0.18380407437219692 0.0 46-47 0.0 0.0 0.0 0.2360906533734796 0.0 48-49 0.0 0.0 0.0 0.29745577487544916 0.0 50-51 0.0 0.0 0.0 0.3645738765182285 0.0 52-53 0.0 0.0 0.0 0.43152205891634243 0.0 54-55 0.0 0.0 0.0 0.506310800746868 0.0 56-57 0.0 0.0 0.0 0.5910034071235972 0.0 58-59 0.0 0.0 0.0 0.6855998780465307 0.0 60-61 0.0 0.0 0.0 0.7887651337361552 0.0 62-63 0.0 0.0 0.0 0.8933382917387205 0.0 64-65 0.0 0.0 0.0 1.005873380062744 0.0 66-67 0.0 0.0 0.0 1.1348420867636793 0.0 68-69 0.0 0.0 0.0 1.2824290878443656 0.0 70-71 0.0 0.0 0.0 1.4436096284982731 0.0 72-73 0.0 0.0 0.0 1.6161019360113253 0.0 74-75 0.0 0.0 0.0 1.7854871487647843 0.0 76-77 0.0 0.0 0.0 1.9652859837984429 0.0 78-79 0.0 0.0 0.0 2.1770781851847896 0.0 80-81 0.0 0.0 0.0 2.409891824554011 0.0 82-83 0.0 0.0 0.0 2.653021989492194 0.0 84-85 0.0 0.0 0.0 2.891952721669364 0.0 86-87 0.0 0.0 0.0 3.136223772964586 0.0 88 0.0 0.0 0.0 3.3375538037151142 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2795 0.0 54.218346 1 GTATCAA 5180 0.0 41.156708 1 ATCAACG 5795 0.0 36.01948 3 TCAACGC 5790 0.0 35.888195 4 CAACGCA 5935 0.0 34.852974 5 AACGCAG 6165 0.0 33.540485 6 TATCAAC 6355 0.0 33.06739 2 CGCAGAG 6970 0.0 29.395603 8 ACGCAGA 7020 0.0 29.321537 7 CGAACCG 65 0.0061476696 28.930407 5 GCAGAGT 8005 0.0 25.183992 9 TACATGG 4820 0.0 24.676378 2 GTACATG 4930 0.0 24.247055 1 TATACCG 160 2.0356201E-6 23.505957 5 ACATGGG 4880 0.0 23.120611 3 GAGTACT 5575 0.0 22.800852 12-13 ATACCGT 280 7.6397555E-11 21.819012 6 CAGAGTA 8015 0.0 21.429522 10-11 AGAGTAC 7330 0.0 20.867752 10-11 ATACCGG 160 5.4201522E-5 20.560223 6 >>END_MODULE